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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#26701449 - 05/28/20 10:18 AM (3 years, 7 months ago) |
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Okay yeah a quick run through the flame is what I do as well. Didn't know if it was too much/too little or anything. Although I use petris now, I did like being able to turn no pours sideways so nothing was over the top during transfers.
And that'd be interesting Crack. Is that the main reason you wanna try out swabs? Or is it to just cut out flame sterilization altogether?
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Quote:
Roger Clemency said: There’s been some conversing about that ring lately, calling it the Halo. I don’t know if there’s a legit term. The Halo sounds pretty good though.
Some people think it’s bad, others see it all the time and never have problems.
Its typically called the leading edge and with healthy mycelium growth can be present but thin: a thicker opaque leading edge is referred to as a halo and is indicative to bacterial presence... bacteria is not necessarily a loss, and I dare say some of my best flushes were from bacterial spawn stress fruiting in response to the infection... bacteria can be combated by going on the dry side with prep and providing plenty of air exchange: conditions some growers provide anyway, so they're less likely to run into issue... if its not moldy or snotty with bacterial metabolites, I RUN DEMZ
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 53 minutes
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Re: Cultivation General Discussion [Re: Caps McGee]
#26701469 - 05/28/20 10:26 AM (3 years, 7 months ago) |
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Yeah, just to get rid of the contamination vector that the handle poses. I don't get allot but when I do. It's one of those side satellites from the handle. But the blade let's you be so much more intricate.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Crackatoa] 1
#26701569 - 05/28/20 11:10 AM (3 years, 7 months ago) |
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Can you use your inoculation loop to transfer instead? If you are punching with D3monic punch Tek, then you have a inoculation loop that gives you more reach and a thinner metal that is much easier to get red hot.
I think I'm the guy who keeps saying a halo is caused by high nutrient hard agar and isn't necessarily mold infront of mycelium.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: Sockadin] 1
#26701598 - 05/28/20 11:23 AM (3 years, 7 months ago) |
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The handle is why I love the #7 scalpel.
I just spray it with iso and wipe it down before I start.
I guess you could always soak a strip of paper towel with iso and wrap it around the part of the handle that goes over the dish.
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LAGM2020     
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 53 minutes
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Re: Cultivation General Discussion [Re: A.k.a]
#26701620 - 05/28/20 11:32 AM (3 years, 7 months ago) |
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I have thought about wrapping the handle in something iso soaked. I do love the #7 handle though. So light and thin. I sometimes use the demonic way, sometimes just cut a wedge with my blade. Either way I transfer with a blade.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Crackatoa] 1
#26701631 - 05/28/20 11:36 AM (3 years, 7 months ago) |
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Why bother with wrapping or any of that?
Here you can see how I flame my #7. The flamed part is 80mm long

Most petri dishes are only 90mm wide

I have 0 issues reaching into petris all when I flame my #7 like this

In between transfers I lay my blade on an iso soaked paper towel which cools it off enough to be flamed again.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: natedawgnow] 1
#26701637 - 05/28/20 11:39 AM (3 years, 7 months ago) |
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I feel like my exacto knife needs an upgrade. I want a #7!
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Sockadin]
#26701652 - 05/28/20 11:47 AM (3 years, 7 months ago) |
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You can get one plus a bunch of blades for 10 bucks on the 'zon.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: natedawgnow]
#26701660 - 05/28/20 11:50 AM (3 years, 7 months ago) |
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Hate the Amazon. But alas, birthday is coming soon.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Sockadin]
#26701676 - 05/28/20 11:55 AM (3 years, 7 months ago) |
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Alternatively you can order one from paul stamets on fungi dot com and pay 20 bucks
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: natedawgnow]
#26701690 - 05/28/20 12:01 PM (3 years, 7 months ago) |
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I feel like I've been in the dark for so long... had no idea that handle exists. #7 scalpel incoming off the 'Zon right now. So do you flame that portion of your handle "red hot" as well? Or just hot enough that it's likely nothing on there is still alive?
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Gan]
#26701692 - 05/28/20 12:04 PM (3 years, 7 months ago) |
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The latter. It'll get red hot but I dont usually flame it that long cause it's not necessary. Definitely flame the blade and blade mounting section red hot though.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Gan]
#26701710 - 05/28/20 12:13 PM (3 years, 7 months ago) |
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Well Amazon got me again. Ended up getting the scalpel some slides and immersion oil. Fuck 30 bucks go e just for free shipping.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Sockadin]
#26701726 - 05/28/20 12:19 PM (3 years, 7 months ago) |
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Yeah I just bought some blades and a #7 handle haha. I always get back at Amazon by abusing their free trial lol. I've probably made like 30 Amazon accounts 
Also, Sock did you ever find a microscope? Saw you were looking for recommendations a bit ago
Edited by Gan (05/28/20 12:25 PM)
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Gan]
#26701771 - 05/28/20 12:45 PM (3 years, 7 months ago) |
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Yeah I ordered AMS B120 based on a Allan recommendation.
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Caps McGee]
#26701776 - 05/28/20 12:47 PM (3 years, 7 months ago) |
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Quote:
gizmo1 said: Idk I wipe the handle and blade before starting then I pass the end of the handle through the flame before every transfer too. Just real quick on both sides I don't heat it till hot or anything like that. Just a quick flame before heating the blade red hot.
That's how I do it too
Quote:
Caps McGee said: and is indicative to bacterial presence...

Transparent halo can be caused by a lot of things. While it may be because of bacteria, it could also be an early culture, soft agar or many other things.
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 12 hours, 16 seconds
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Re: Cultivation General Discussion [Re: Caps McGee]
#26701791 - 05/28/20 12:52 PM (3 years, 7 months ago) |
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Quote:
Caps McGee said: a thicker opaque leading edge is referred to as a halo and is indicative to bacterial presence...
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Camera93]
#26701805 - 05/28/20 01:00 PM (3 years, 7 months ago) |
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That's fair but I did see a post someone did today of a clear translucent halo which I also thought was a mold culture running ahead of the myc.
I think it's probably both. Most of the time I'm gonna lean to the nutrient density of the agar argument. But sometimes it is mold. I don't know that I have seen bacteria make a halo ahead of Mycelium because bacteria seems slower to me. Like it doesn't reach out for nutes it just pools and copies. But WTF do I know. I'm just a dude with a hobby. Some people on here are actual mycologists.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 53 minutes
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Re: Cultivation General Discussion [Re: Sockadin]
#26701825 - 05/28/20 01:11 PM (3 years, 7 months ago) |
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I bought mine from the zon but they didn't send the right blades. They sent big ass blades, luckily I still had some nice skinny blades, I forget which number
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