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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Gan]
    #26699943 - 05/27/20 04:58 PM (3 years, 7 months ago)

That seems fine to me.

If you ever catch a spore plate when it’s just germinated the growth is clear like that until it thickens up.


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OfflineGan
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Re: Cultivation General Discussion [Re: A.k.a]
    #26699963 - 05/27/20 05:11 PM (3 years, 7 months ago)

Maybe I'm just over thinking it. I'm thinking of transfers from 2 to 8 o'clock on plate 1 and between 4 to 7 o'clock on plate 2. If you have any suggestions for better places for transfer, I'm all ears. This is some of the first agar work I've done in months.

Also, I've always been under the assumption to take a transfer as soon as mold shows up anywhere on a plate. My thinking is that as soon as the mold sporulates, I'd assume everything inside the plate will be covered with spores. However, I've never really tested that theory. Is that actually true? Ideally, I'd like to let these plates grow out another 24 hours, but due to the mold sites on both plates I feel like I should transfer them tonight.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Gan]
    #26699976 - 05/27/20 05:16 PM (3 years, 7 months ago)

The very first pic I would grab from around 9 myself.

I’ve had mixed results transferring from plates with sporulating mold.  Sometimes it seems to be clean and sometimes I’ll get some mold popping up on the new plate a few days later.

I generally try to do it ASAP too though. Just cuz some appeared to be clean on the next plate doesn’t mean there aren’t mold spores hiding in there somewhere.


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: A.k.a]
    #26700184 - 05/27/20 07:09 PM (3 years, 7 months ago)

I have a bacterial jar that I wanted to case and top fruit. For coir how deep should I make the casing? The grains are a little higher on one side if that makes any difference


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InvisibleCulliganman360
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #26700468 - 05/27/20 09:23 PM (3 years, 7 months ago)

Grain strainer for bulk..



Too bad it takes forever to dry em .
Goin on 2 hr now


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Invisiblefilthyknees
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Re: Cultivation General Discussion [Re: Culliganman360]
    #26700479 - 05/27/20 09:29 PM (3 years, 7 months ago)

Put a box fan underneath pointing up.


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InvisibleHobbit GDF
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Re: Cultivation General Discussion [Re: filthyknees]
    #26700502 - 05/27/20 09:40 PM (3 years, 7 months ago)

Some times I put fan right on top of them and blow through them. Mix it ever 30 min until dry. Dont take to long that way.


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InvisibleCulliganman360
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Re: Cultivation General Discussion [Re: Hobbit GDF]
    #26700511 - 05/27/20 09:51 PM (3 years, 7 months ago)

Thanks y’all 🤟


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: TedsDead]
    #26700527 - 05/27/20 10:02 PM (3 years, 7 months ago)

Quote:

TedsDead said:
Quote:

gizmo1 said:

This tubs look a little off to anyone else
Its got 3 pins and no visable knots. Probably  my fault for running untested clones lmao.
Anything I should do? Increase FAE? I took the filters out of top two holes.





those also look a bit overhydrated to me.  look out for trich



Cool.
It does look wet. I use 1 quart water to one brick and I'm still getting water gushing out when I squeeze lmao I had to use a bunch of verm to dry it out.
I'm just tired of mold it's been a never ending battle since I moved into this house I finally thought I beat it and boom summer hits fruit flys are back and contamination goes back up. It don't matter I won't let it beat me.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Culliganman360]
    #26700536 - 05/27/20 10:06 PM (3 years, 7 months ago)

Quote:

Culliganman360 said:
Grain strainer for bulk..



Too bad it takes forever to dry em .
Goin on 2 hr now




if you have the space try using a 12'x6'  canvas drop cloth and use a rake to spread it out and help evaporation.  I can dry 50lb's of grain in a couple hours with no fans just a couple passes with the rake


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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InvisibleCulliganman360
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Re: Cultivation General Discussion [Re: TedsDead]
    #26700559 - 05/27/20 10:18 PM (3 years, 7 months ago)

I thought some cloth might make em stay wet bro . But does anyone reuse poly stuffed lids? Or reuse ez felt lids? there like 3-4 months old tops . Only been thru a handful of Pc runs


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OfflineGan
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Re: Cultivation General Discussion [Re: Culliganman360]
    #26701114 - 05/28/20 07:22 AM (3 years, 7 months ago)

I refuse reuse my poly lids fairly often. No real problems. But I make sure to always check if they need replaced or just replace them once they start looking rough. I switched to sfd's so I didn't have to remake poly lids though

Edit: reuse, not refuse


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Edited by Gan (05/28/20 09:42 AM)


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Gan] * 1
    #26701272 - 05/28/20 08:57 AM (3 years, 7 months ago)

What's people's ideas on the flameless transfer? Transferring from plate to plate with a sterile swab instead of a blade.


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OfflineGastronomicus
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26701282 - 05/28/20 09:06 AM (3 years, 7 months ago)

Day 5 and no growth yet on my agar plates. No mold either so that's good. These spores are slooooow though. Or maybe I'm just impatient


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Gastronomicus]
    #26701303 - 05/28/20 09:17 AM (3 years, 7 months ago)

That would save some time but it seems like you’d burn through swabs.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: A.k.a]
    #26701358 - 05/28/20 09:43 AM (3 years, 7 months ago)

A swab seems kinda sloppy.
I mean I'm sold on taking the smallest bit, even a single strand of mycilium.


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OfflineGan
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Re: Cultivation General Discussion [Re: A.k.a]
    #26701407 - 05/28/20 09:58 AM (3 years, 7 months ago)

What's your all's thought on the sterility of the scalpel handle? When placing a transfer on a blank plate, roughly 1/4" or so of the handle comes over the dish to place it in the middle of the plate. At the start of a session I'll flame the first inch or so of the handle where it meets the blade until it's pretty damn hot. Then flame it 1quickly while flaming the blade red hot. Do y'all think it's an issue to have a little of the handle over the plate like that? It's never caused me issues that I can tell.


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: Gan] * 2
    #26701419 - 05/28/20 10:04 AM (3 years, 7 months ago)

Idk I wipe the handle and blade before starting then I pass the end of the handle through the flame before every transfer too. Just real quick on both sides I don't heat it till hot or anything like that. Just a quick flame before heating the blade red hot.


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InvisibleJust_A_Noob
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Re: Cultivation General Discussion [Re: Gan] * 1
    #26701420 - 05/28/20 10:04 AM (3 years, 7 months ago)

I’ll just wipe it with some iso before I start.  If you flame until the handles hot that’s too much.  ime/o


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Gan]
    #26701423 - 05/28/20 10:05 AM (3 years, 7 months ago)

That's my thoughts, I flame up my handle. You wouldn't have that with a swab. But you would run through quite a bit, especially when you got several things going at once.


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