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Invisiblesh4d0ws
LSx
I'm a teapot User Gallery

Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: LogicaL Chaos] * 1
    #26660011 - 05/09/20 01:08 PM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
Nice! How much did your flowhood cost?





About $650 all said and done. I bought basically everything new. In the future I would probably call around to hvac places and find a blower for free/cheap rather than go the route I did. I used an inline fan, which is similar to this build from fungifun.org



http://www.fungifun.org/English/Flowhood

(thanks Anno!!)

I got the specs for the fan and made sure it worked with the filter I purchased and everything first. But it cost me around $200 for it, and it's pretty easy to find used blowers for a lot less.

Also I think filters are generally more expensive in Canada. I ended up forking out $350 on Ebay for the filter I got. a 24" x 24" so I could do bags.

I had looked at a 12" by 24" for a bit but I think it was boogieman who suggested to grab the 24" ...thanks boogie :super:


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InvisibleRyeHumor
MasterProcrastinator
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Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: Cultivation General Discussion [Re: sh4d0ws]
    #26660047 - 05/09/20 01:25 PM (3 years, 8 months ago)

So I know bigger is better when it comes to flow hoods :naughty:

But I'm already pushing it with the amount of space that my cult stuff is taking up in my place (according to the boss)

Before anyone shuts it down completely...

Is there any merit to making a mini flow hood just for doing agar?

Something small enough or easy enough to disassemble and store?

I'm thinking I might be able to design one that the blower can fit inside of to be stored in the closet when not in use.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #26660190 - 05/09/20 02:42 PM (3 years, 8 months ago)

I've found a Canadian manufacturer that builds reasonably priced high efficiency HEPAs (99.9995% @ .1 microns)my 24x48 cost $536.75 tax included. Not too bad.


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Offlinepoisoned
untitled
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Registered: 04/17/13
Posts: 1,738
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Re: Cultivation General Discussion [Re: RyeHumor]
    #26660195 - 05/09/20 02:43 PM (3 years, 8 months ago)

I feel like flowhood is way more useful in all other transfers. I don't care nearly as much about plates going to shit vs jars/bags going to shit.


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How I do glass dishes


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OfflineYeetusdeetus
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Registered: 11/23/19
Posts: 1,242
Last seen: 6 hours, 35 minutes
Re: Cultivation General Discussion [Re: poisoned]
    #26660459 - 05/09/20 05:11 PM (3 years, 8 months ago)

Re-melted agar to pour some plates, forgot about it for a little too long and some of the plates came out chunky:mad2:. Had good technique pouring but agar chunks are touching the lids on some of the plates. Think they’re good or should I just toss those?


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #26660464 - 05/09/20 05:13 PM (3 years, 8 months ago)

I use chunky plates for spores or early transfers usually.


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LAGM2020


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: A.k.a]
    #26660515 - 05/09/20 05:37 PM (3 years, 8 months ago)

I’ve got some atl spores I wanna try out but I’ve only got one print and I don’t wanna fudge it up trying to save a couple petris lol. I’ll try em for this set of transfers and hope for the best.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #26660517 - 05/09/20 05:40 PM (3 years, 8 months ago)

I just got atl recently myself.

The print was super faint so I was worried too lol.

They ended up going off on two plates in under two days.


This was at like 30 hours. Blew my mind.


I think spores must like heat cuz I swiped atl7, tamp, golden halo, Ecuador and b+ and all of them germinated by two and a half days. When I started using prints for lagm in January it took way longer.


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LAGM2020


Edited by A.k.a (05/09/20 05:41 PM)


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: A.k.a]
    #26660538 - 05/09/20 05:54 PM (3 years, 8 months ago)

Do you know when the prints were made? I’ve heard fresher spores tend to germ a lot sooner. I’m so bad at seeing good growth from spores in my sab lol.Thinking about just using a loop for the first couple transfers so I don’t have to fumble around taking the tiniest transfers with a scalpel:tongue:. Haven’t actually tried using a loop for transfers yet though so idek if it’d grow right without the wedge.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #26660546 - 05/09/20 05:58 PM (3 years, 8 months ago)

Idk, but I’ve used spores less than 12 hours old a couple times and they grew pretty much overnight.

I use a loop. I make softer plates then torch the loop, dip it in the plate to cool it and make it sticky and then rub it on the print.

I’ll use a scalpel on really heavy prints where you can just pick up flakes of spores but for normal ones it’s too likely to rip the foil.


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LAGM2020


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: A.k.a]
    #26660647 - 05/09/20 06:54 PM (3 years, 8 months ago)

Sorry I’m a little sleep deprived, I meant a loop to transfer myc away from contams lol. I don’t have much experience with spores but I’ve only used a loop for em and it usually results in at least some cleanable myc. I just feel like it’d be quicker to scoop a little bit of myc with a loop and plop it on a new plate to get away from the bad stuff than having to make multiple fine cuts and potentially have the wedge slip off the blade back onto the plate and possibly get contaminated. Plus there’s probably fewer genetics when using a loop to transfer.


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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26660654 - 05/09/20 06:56 PM (3 years, 8 months ago)

Quote:

Sockadin said:
I would definitely clone the little guy. We are all more interested in the gnome shroom you got there I'm guessing.

Man AA  toad stools would be a fun gnentic to play with.

I'm guessing the little brown pin is just the genetic randomness of AA , I mean they use to have brown caps before it got bread out of them right?





thats what i was thinking, i cloned both :smile:

Quote:

p9hu7 said:
My hood is my most prized possession.




me too, im in love with mine. i might marry it


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #26660660 - 05/09/20 07:01 PM (3 years, 8 months ago)

C10 is that animation in your signature with the plate a method to dilute spores?


The way my streak plates have gone I can’t imagine getting growth past the third section. I’m gonna try it though just to see what happens.

Plus it’d be cool to narrow genetics down right off the bat and save some time.


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LAGM2020


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: A.k.a] * 1
    #26660791 - 05/09/20 08:13 PM (3 years, 8 months ago)

Yep! basically serial dilution on the surface. I detail it in my agar guide in my sig

I love this method. It gives you cleaner growth and more unique colonies to choose from right off the bat. Ive gotten an isolate in 4 transfers this way before

Sometimes you only get growth in zone 1, other times every zone, depending on spore viability and # of spores

Here are some examples of what it looks like



--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Cultivation General Discussion [Re: c10h12n2o] * 2
    #26661049 - 05/09/20 10:19 PM (3 years, 8 months ago)

I have used this quarantine to inoculate 69 jars of WBS.


G2G is fucking amazing, y'all.


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:nyan: <-- Clicky Clicky


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InvisibleHobbit GDF
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Re: Cultivation General Discussion [Re: ShroomerInTheRye] * 2
    #26661307 - 05/10/20 12:36 AM (3 years, 8 months ago)

Quote:

ShroomerInTheRye said:
I have used this quarantine to inoculate 69 jars of WBS.


G2G is fucking amazing, y'all.



That's how I been spending my time at home too
G2G is my preferred method also. I love using bags now and using my qts for masters.
Plus I love using 1/2 gal jars and big pickle jars.


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OfflineLogicaL ChaosM
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Re: Cultivation General Discussion [Re: ShroomerInTheRye] * 1
    #26661357 - 05/10/20 12:58 AM (3 years, 8 months ago)

Do u say 69 grain jars were handled?!

:wooyeah: :douchewink:


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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InvisibleShroomerInTheRye
Clit Commander
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Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Cultivation General Discussion [Re: LogicaL Chaos] * 1
    #26661799 - 05/10/20 07:33 AM (3 years, 8 months ago)

Hell yeah brother!  69!

:crondance: :hotdamn:

I would have kept going but I ran out of jars.  :sad:


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:nyan: <-- Clicky Clicky


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OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 11 days, 11 hours
Re: Cultivation General Discussion [Re: ShroomerInTheRye] * 1
    #26661902 - 05/10/20 08:58 AM (3 years, 8 months ago)

Quote:

ShroomerInTheRye said:
I would have kept going but I ran out of jars.  :sad:




I know that feel brah, i just used up all my jars too! Btw keep an eye on those shelves, they look like they're bowing a little to me.


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Offlinekaphaow
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Last seen: 3 years, 5 months
Re: Cultivation General Discussion [Re: MLPismyOPSEC] * 1
    #26662019 - 05/10/20 10:01 AM (3 years, 8 months ago)

My first attempt with B+ MS syringe to agar. Plates were started on 4/27 so 13 days ago. My goal is to make some LC for inocculating BRF cakes. Can I take one of these plates and make the LC or should I transfer? I'm not sure which part to transfer, need some advice.



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