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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: poisoned] * 1
    #26650082 - 05/05/20 08:31 AM (3 years, 8 months ago)

Yeah I have grain soaking for 7 or 8 quarts right now so I’m gonna throw a couple moldy ones in with them next cycle and see how they turn out.

lol prob add another hour too just because they’re gross and I hate them.


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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: A.k.a]
    #26650108 - 05/05/20 08:46 AM (3 years, 8 months ago)



Heres my blobbing substrates. Someone said that over nuting my spawn could be the culprit. I soaked these in coffee and a teaspoon of gypsum for 12 hours. Then didnt even drain it and simmerd it on the stove and i think it might have been a little much.

I know its not just isolated to the tub because it did it on a grain a cake I was also fruiting. I threw it away. Any helpful recommendations would be appreciated. Also might it just be my culture im working with? How often does it happen that you isolate a culture that only produces blobs? If its not my culture ill just make new grain with lower ammounts of coffee and gypsum


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26650123 - 05/05/20 09:00 AM (3 years, 8 months ago)

Who knows about the ks/ksss varieties??

I’ve been searching threads all morning but it’s hard to find much and what I did find people didn’t seem to be sure what they were actually growing.

So-
Ks is more normal cube looking
Ksss is mostly short wide round ones right??

Then what’s ufo normally look like? That’s the one I can’t find anything at all on. I know the ufo I’ve got going came from what was basically caps growing on the sub, but idk if that’s typical. The plate pins look like ksss, short and round.

Then there’s potency, the stuff I just read had equal amounts of people saying it’s got mutant potency like PE, and other people saying it’s average at best.


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Offlinekaphaow
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Re: Cultivation General Discussion [Re: A.k.a]
    #26650476 - 05/05/20 11:38 AM (3 years, 8 months ago)

Question was more if it's mycelium that is indeed growing or if it's a contamination? I have 4 cups and they all look the same. Planning on transferring today.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: kaphaow]
    #26650528 - 05/05/20 11:55 AM (3 years, 8 months ago)

Looks like myc but it could be mold.  Sometimes myc from spores looks all curly and random like that though.


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Invisiblesh4d0ws
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Re: Cultivation General Discussion [Re: A.k.a]
    #26650537 - 05/05/20 11:57 AM (3 years, 8 months ago)

Quote:

A.k.a said:
Who knows about the ks/ksss varieties??

I’ve been searching threads all morning but it’s hard to find much and what I did find people didn’t seem to be sure what they were actually growing.

So-
Ks is more normal cube looking
Ksss is mostly short wide round ones right??

Then what’s ufo normally look like? That’s the one I can’t find anything at all on. I know the ufo I’ve got going came from what was basically caps growing on the sub, but idk if that’s typical. The plate pins look like ksss, short and round.

Then there’s potency, the stuff I just read had equal amounts of people saying it’s got mutant potency like PE, and other people saying it’s average at best.





I thought it was all KSS and that there is just different phenos, the UFO pheno and some more normal looking fruits sometimes

I might be wrong though and also there might still be a KS variety cause koh samui super is the full length? so is there just a koh samui? May have spelled that wrong lol


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #26650570 - 05/05/20 12:08 PM (3 years, 8 months ago)

I believe there to be KS, KSSS and the KSSS UFO pheno. I can't spell it for nothing. I've got prints labeled as all 3 anyway. Only fruited the UFOs though.


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Edited by Crackatoa (05/05/20 12:10 PM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26650609 - 05/05/20 12:21 PM (3 years, 8 months ago)

Whatd your ufo turn out like?

I just spawned my first one yesterday and I think I’ve got either ks or ksss on the way.


Yeah it’s ks super strain is what ksss stands for.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: A.k.a]
    #26650679 - 05/05/20 12:47 PM (3 years, 8 months ago)


Short and fat. Didn't ever try them either. Still got em though.


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InvisibleHobbit GDF
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26651175 - 05/05/20 04:29 PM (3 years, 8 months ago)

Here is today's pictures.
This is mazapetek.  Cool little mutant


And

Tailfeathers3 & white tailfeathers1
+ a 3d poster from dead lot



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InvisibleRyeHumor
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Re: Cultivation General Discussion [Re: gizmo1]
    #26651212 - 05/05/20 04:47 PM (3 years, 8 months ago)

Quote:

gizmo1 said:
Quote:

TedsDead said:
bleach has to sit on the surface for 10 minutes to kill everything tho so consider that.  maybe a stronger peroxide solution but that stuff burns



Same thing with ISO it doesnt work instantly. The wiping is what does the work in our case and iso works good because it evaporates fast. Bleach water will work just as good but wont evap as fast. The wiping motion will take off any loose dust or settled spores that could fall into a opened jar or dish.
You could also just skip using a sanitizer and wiping all together especially if you are using right away and not letting stuff sit around where dust can settle on it.




So then soapy water could work just as well if you let it evaporate spray and wipe it down?


Edited by RyeHumor (05/05/20 05:58 PM)


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: RyeHumor]
    #26651282 - 05/05/20 05:15 PM (3 years, 8 months ago)

70% alcohol is recommended to kill bacteria and to use as a disinfectant. This is because the added water content slows down the evaporation rate which means the isopropyl alcohol has longer contact with the surface, increasing the effectiveness of killing the germs.

https://www.sydneysolvents.com.au/will-isopropyl-alcohol-kill-germs


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26651342 - 05/05/20 05:59 PM (3 years, 8 months ago)

That’s cool I didn’t know that.

I had only seen it was that the water content made it able to penetrate cell walls easier.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: A.k.a]
    #26651377 - 05/05/20 06:13 PM (3 years, 8 months ago)

Yeah, I've read that as well.


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InvisibleRyeHumor
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Re: Cultivation General Discussion [Re: A.k.a]
    #26651394 - 05/05/20 06:21 PM (3 years, 8 months ago)

Interesting I did find a paper on the subject of IPA alternatives here's an excerpt from the conclusion.

"The best alternative from an overall health and environmental standpoint is hydrogen
peroxide. This chemical is used in either a 3% or 6% concentration in water today and, in
contrast to IPA, it is capable of controlling spores in addition to bacteria. IRTA and the group
decided to select 3% hydrogen peroxide as a candidate for testing and wanted to also test 1%
hydrogen peroxide to determine if it had disinfecting capability. The aim was to use as dilute a
concentration as possible."

Source:
K. Wolf, “Alternatives to Isopropyl Alcohol for Biocide Control in ...,” Jun-2013. [Online]. Available: http://www.irta.us/reports/BAAQMDIPAAltintreptdraft.pdf. [Accessed: 06-May-2020].

"Hydrogen peroxide is active against a wide range of microorganisms, including bacteria, yeasts, fungi, viruses, and spores 78, 654. A 0.5% accelerated hydrogen peroxide demonstrated bactericidal and virucidal activity in 1 minute and mycobactericidal and fungicidal activity in 5 minutes."

"A 3% concentration for 150 minutes killed 106 spores in six of seven exposure trials "

"A 3.0% hydrogen peroxide solution was ineffective against VRE after 3 and 10 minutes exposure times and caused only a 2-log10 reduction in the number of Acanthamoeba cysts in approximately 2 hours 662"

"The time required for inactivating three serotypes of rhinovirus using a 3% hydrogen peroxide solution was 6–8 minutes."

Source:
“Chemical Disinfectants,” Centers for Disease Control and Prevention, 18-Sep-2016. [Online]. Available: https://www.cdc.gov/infectioncontrol/guidelines/disinfection/disinfection-methods/chemical.html. [Accessed: 06-May-2020].


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: RyeHumor]
    #26651446 - 05/05/20 06:41 PM (3 years, 8 months ago)

Quote:

RyeHumor said:
Quote:

gizmo1 said:
Quote:

TedsDead said:
bleach has to sit on the surface for 10 minutes to kill everything tho so consider that.  maybe a stronger peroxide solution but that stuff burns



Same thing with ISO it doesnt work instantly. The wiping is what does the work in our case and iso works good because it evaporates fast. Bleach water will work just as good but wont evap as fast. The wiping motion will take off any loose dust or settled spores that could fall into a opened jar or dish.
You could also just skip using a sanitizer and wiping all together especially if you are using right away and not letting stuff sit around where dust can settle on it.




So then soapy water could work just as well if you let it evaporate spray and wipe it down?



Alot of people don't even wipe jars and plates anymore.
You could probably even use straight water, but yes soapy water will work you don't want alot of soap in it maybe a couple two or three drops. Yes I said couple two or three just the way I talk lmao.
Soap is a pretty damn good antibacterial on it's own without any additives. I read a a post from someone I think it was Bod talking about how soap itself is more antibacterial than whatever they add to it to make it antibacterial and that soap rips apart cell walls killing bacteria. Or some shit like that :shrug:


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: RyeHumor]
    #26651447 - 05/05/20 06:42 PM (3 years, 8 months ago)

Bacteria under the scope. Bleach, soap, 3% H202, salt, 70% iso.



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OfflineCactiChris
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26651477 - 05/05/20 07:00 PM (3 years, 8 months ago)

So just random question for everyone, how long do you use your pressure canner seal and blow out plug before you replace it?


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26651480 - 05/05/20 07:02 PM (3 years, 8 months ago)

Quote:

p9hu7 said:
Bacteria under the scope. Bleach, soap, 3% H202, salt, 70% iso.





Scary shit. lmao.
Notice how nost of those are sped up though. Sanitizers take more time to work than how long we use them for we just do a quick wipe and thats enough to remove them altleast the loose stuff from the surface and thats all thats needed. Even then sometimes thats not even needed thats why I say it doesn't really matter what we yse we arent soaking stuff in iso long enough to kill mold and bacteria anyways we are just taking away the loose shit so it cant fall into our jars and plates. Wiping is the real star here not what we wipe with.


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: CactiChris] * 1
    #26651486 - 05/05/20 07:05 PM (3 years, 8 months ago)

Quote:

CactiChris said:
So just random question for everyone, how long do you use your pressure canner seal and blow out plug before you replace it?



I've got a presto that has a 8 year old seal and plug. The seal leaks a shitload at first but will stop once pressure starts building its definitely ready to be replaced. If I soak it in hotwater ahead of time it doesn't leak at all. I just kinda avoid using that pressure cooker most of the time lmao.


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