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OfflineSloppyJoseph
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Re: Cultivation General Discussion [Re: gizmo1] * 1
    #26636571 - 04/29/20 11:05 AM (3 years, 8 months ago)

If you are taking prints, then yes great opportunity, but if you are not and you dont want a bunch of loose caps.. why not just harvest the big ones? I usually harvest my tubs in 3 turns per flush because that's how I usually experience the maturities in each flush. So the big ones get picked in the morning, the next wave gets picked that night, then the last round gets picked the following morning. That's the usual timeframe for me


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OfflineThrill
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Re: Cultivation General Discussion [Re: SloppyJoseph]
    #26636702 - 04/29/20 11:59 AM (3 years, 8 months ago)

Gonna be PCing some agar in a lil bit. Wanna throw some old LC syringe tips in to sterilize too. Theyre already pre sterilized in the plastic hub, but theyre like 7 years old and been knocked around. You think half an hour is long enough to sterilize the hub? Theyre pretty heavy duty needles with a pretty solid plastic cover and cap.


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Offlinepoisoned
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Re: Cultivation General Discussion [Re: Thrill]
    #26636754 - 04/29/20 12:26 PM (3 years, 8 months ago)

20mins at 15 psi should do it.


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Thrill]
    #26636799 - 04/29/20 12:46 PM (3 years, 8 months ago)

Quote:

Thrill said:
Gonna be PCing some agar in a lil bit. Wanna throw some old LC syringe tips in to sterilize too. Theyre already pre sterilized in the plastic hub, but theyre like 7 years old and been knocked around. You think half an hour is long enough to sterilize the hub? Theyre pretty heavy duty needles with a pretty solid plastic cover and cap.



The hub or other parts that don't go in direct contact with the substrate don't need to be sterilized only sanitized so wiping with ISO is fine, and the needle will get sterilized in the flame. Altho you could also just boil the parts for a few minutes in sterile water that would work as well. (you'd still have to flame sterilize the needle tho) but I also doubt that you will make it more sterile than what it already is even tho its 7 years old. That's just my 2c


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OfflineThrill
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Re: Cultivation General Discussion [Re: Thrill]
    #26636830 - 04/29/20 01:08 PM (3 years, 8 months ago)

These are for aspirating LCs. They are meant to be installed in a jar lid as an access point into an LC and have an insert that slips inside the needle and the top of the hub to keep contams out.


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: Thrill]
    #26636907 - 04/29/20 01:52 PM (3 years, 8 months ago)

it's probably still sterile. I just used some very old expired syringe and needles the other day for lcs and all test plates came out clean I was concerned at first but it all worked out great. For stuff like that as well as swabs I go about 15 minutes at 15 psi.


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OfflineThrill
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Re: Cultivation General Discussion [Re: gizmo1]
    #26636936 - 04/29/20 02:05 PM (3 years, 8 months ago)

I just skipped them. I never realized but looking at them closer they have "dispose after use" printed on the side, so they might end up melting into a big disappointing blob of shit in a PC. I know a bunch still have the seal intact so they might still be sterile, and Ive got a box of smaller 1 1/2" hubs still sealed thatll work out for other shit.


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InvisibleRyeHumor
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Re: Cultivation General Discussion [Re: Thrill]
    #26636943 - 04/29/20 02:12 PM (3 years, 8 months ago)

If you've got a couple plates to spare you could grab a couple of needles randomly and place down some water from a sterile syringe.

See if it grows anything.

But at a certain point you could probably just order a bunch more if you're worried right?


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OfflinePsyche delics
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Re: Cultivation General Discussion [Re: Thrill]
    #26636960 - 04/29/20 02:21 PM (3 years, 8 months ago)

.


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Edited by Psyche delics (04/29/20 03:01 PM)


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OfflinePsyche delics
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Re: Cultivation General Discussion [Re: Psyche delics]
    #26636973 - 04/29/20 02:31 PM (3 years, 8 months ago)

.


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Edited by Psyche delics (04/29/20 03:01 PM)


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InvisibleAlkeides
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Re: Cultivation General Discussion [Re: Psyche delics]
    #26637397 - 04/29/20 06:31 PM (3 years, 8 months ago)

Hey why does nobody use peroxidated agar anymore?

It appears this forum has been scrubbed of most teks. Here's one I found, but it even is ripped from another forum and the poster has been scrubbed. It also isn't very scientific about concentration but from what I can glean from responses to scrubbed teks, preferred concetration is 1:20 of 3%USP H2O2 to agar recipe.

I'm working on a project trying to get some very very dirty cultures cleaned up and was considering peroxidated agar. Am I missing something? Should I not be considering peroxide?


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Invisiblesh4d0ws
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Re: Cultivation General Discussion [Re: Alkeides]
    #26637487 - 04/29/20 07:17 PM (3 years, 8 months ago)

What purpose is the peroxide for?

I have never heard of peroxidated agar? I haave heard of people using agar with antibiotics added but even then, it seems rare and only experienced people who use it. And plenty of people with shit results :shrug:

I don't use peroxide for anything cult related fwiw


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: sh4d0ws] * 1
    #26637531 - 04/29/20 07:40 PM (3 years, 8 months ago)

Peroxidated agar us outdated bc there are better options available... the peroxide WILL get rid of bacteria and competitor molds, but will also cause your culture a great deal of distress as IT is a mold as well... gentamicin sulfate is a powdered antibiotic you can buy for fish tanks and works great... that said, 3% solution at 0.1% or 6% solution at 0.05% will work, and any culture that makes it will be the stronger genetics in the plate


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InvisibleAlkeides
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26637555 - 04/29/20 07:55 PM (3 years, 8 months ago)

Cool thank you for the recommendation I'll look more into gentamicin


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Invisiblesh4d0ws
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Re: Cultivation General Discussion [Re: Alkeides] * 1
    #26637563 - 04/29/20 07:58 PM (3 years, 8 months ago)

Also I forgot to mention, Some people have had success using black tea agar as well, apparently it has some natural antibiotics / antibacteria components

Could be worth at a try. I think a couple people posted their resuts on the forums, you might be able to find the posts in the search


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #26637633 - 04/29/20 08:40 PM (3 years, 8 months ago)

I don't think peroxide works well it supposedly breaks down when introduced to light and the heat I'm guessing isn't great for it either.
What contaminates are you trying to deal with? If it's bacteria I'd suggest a hot pour. If you are dealing with mold just transfer away from it then a hot pour will work to clean up any bacteria. If you are dealing with a culture that you believe has mold meshed into it the best step to take would be to start again from spore and transfer before it has a chance to get that bad.
If you do end up going with antibiotics or peroxide make sure you finish off on regular agar before going to grain to make sure the bacteria isn't still present and being covered up.


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Edited by gizmo1 (04/29/20 08:42 PM)


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InvisibleAlkeides
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Re: Cultivation General Discussion [Re: gizmo1] * 1
    #26637729 - 04/29/20 09:39 PM (3 years, 8 months ago)

You can check out my project here, I'm attempting to restart a culture from old caps that had been frozen.

I anticipated contams but man I may have bit off more than I can chew. I'm due to post an update soon but I have a busy few days ahead of me so it may be a little while before I can show you folks just how bad these plates are.

Upon further research, peroxidated agar doesn't kill already germinated fungal contams as they can produce the peroxidase to break it down the same way cube mycelium can. It would only help me clear out bacterial and new ungerminated contams, but about 85% of what I'm seeing is definitely germinated fungal probably either penicillium or dactylium.

I had not come across the hot pour technique, I may have to try that...


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: Alkeides] * 1
    #26637880 - 04/29/20 11:18 PM (3 years, 8 months ago)

Use the streaking technique in my sig and you probably wont need antibiotics at all


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Offlinepoisoned
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Re: Cultivation General Discussion [Re: c10h12n2o] * 1
    #26637978 - 04/30/20 12:31 AM (3 years, 8 months ago)

I'd highly recommend you to get some proper plates for such a cleanup. IMO, it's critical that you can see any small amounts of growth of your culture as well as contaminants, so you can transfer at the right time and place.


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: poisoned]
    #26638016 - 04/30/20 12:59 AM (3 years, 8 months ago)

Quote:

poisoned said:
I'd highly recommend you to get some proper plates for such a cleanup. IMO, it's critical that you can see any small amounts of growth of your culture as well as contaminants, so you can transfer at the right time and place.



Good visibility will make a big difference in this case. I like to always keep a stack on hand even when using primarily no pours which I haven't done in awhile. I need to though I feel like I'm getting soft being spoiled by all this visibility lmao.


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