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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23951758 - 12/23/16 01:54 PM (7 years, 1 month ago)

Quote:

ComebackKid said:
Quote:

FriedEgg said:
anyone else use "press n seal" wrap instead of standard saran wrap for agar plates?



https://www.amazon.com/Glad-Pressn-Seal-Wrap-Square/dp/B0014D5NVS




What do you do? Take the whole roll out and chop it into little rolls on a mitre saw?



In the past I've used a D saw to cut them into 1 inch rolls, but never got a super good clean cut, so I didn't make a habit of using it over parafilm


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: filthyknees]
    #23951767 - 12/23/16 01:59 PM (7 years, 1 month ago)

I wonder if a chop saw would cut them any nicer. If you went through real slow maybe? Someone should try it out :awesomenod:
I would myself but I'm still working with no pour

What's the difference between parafilm and cling film anyway?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23951797 - 12/23/16 02:13 PM (7 years, 1 month ago)

I just use a really sharp chefs knife and it cuts rolls just fine :shrug:


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InvisibleFriedEggS
I'm a teapot

Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Cultivation General Discussion [Re: ComebackKid]
    #23951839 - 12/23/16 02:35 PM (7 years, 1 month ago)

Quote:

ComebackKid said:

What do you do? Take the whole roll out and chop it into little rolls on a mitre saw?



yes. you chop up the whole roll into 2" or 3" sections. a serrated knife works fine. then you wrap the saran wrap around the edges of your agar plate. it seals it up while still allowing gas exchange. it works the same as parafilm. but parafilm is expensive.

i've been doing fine with saran wrap but it slips off the glass if your hands are too wet with alcohol. i'm just wondering if anyone has tried press n seal before. anyone?


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InvisibleFriedEggS
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Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Cultivation General Discussion [Re: FriedEgg]
    #23951844 - 12/23/16 02:38 PM (7 years, 1 month ago)

there's a huge difference between saran wrap and press n seal. press n seal has adhesive on it. saran wrap doesn't.


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Offlineenlightenment
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Re: Cultivation General Discussion [Re: FriedEgg]
    #23951846 - 12/23/16 02:39 PM (7 years, 1 month ago)

press n seal is not available where I live but from the picture it looks like it works like parafilm.


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InvisibleFriedEggS
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Re: Cultivation General Discussion [Re: enlightenment]
    #23951848 - 12/23/16 02:42 PM (7 years, 1 month ago)

yup that's exactly why i picked it. but i think the underside of parafilm is sterile. i'm not sure if press n seal is.


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OfflineNDStepp84
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Re: Cultivation General Discussion [Re: FriedEgg]
    #23951878 - 12/23/16 02:54 PM (7 years, 1 month ago)

People have used grafting tape (made by parafilm) with good results and is cheaper at around 8$ 90 feet on Amazon  Just throwing it out there incase someone may find it useful https://www.shroomery.org/forums/showflat.php/Number/19052762


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: NDStepp84]
    #23951985 - 12/23/16 03:37 PM (7 years, 1 month ago)

Bought a 20lb bag of penningtons WBS earlier and a bag of poly. Got my SFD lids ready.... most of you guys soak but no simmer yeah? I used to soak and simmer, get a lot of burst and somewhat starchy seed that way it seems. I dunno.


Ready to try rocking it again though let's do it. Got a nice 4th transfer KSSS plate to inoculate with.


--------------------
KSSS And PE WBS.
 


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Invisiblefilthyknees
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23951997 - 12/23/16 03:41 PM (7 years, 1 month ago)

for wbs foomans tek has been a standard for years and years. would never complicate wbs prep beyond foomans tek.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: Kenetic]
    #23952017 - 12/23/16 03:54 PM (7 years, 1 month ago)

Quote:

kenetic said:
no, just parafilm.





What he said!

At the beginning I also used saran wrap or similiar products. But hey, 30m of Parafilm cost around 30 euros in Germany, it makes more than 1000 dishes which is 3 cent per dish.

I haven't calculated it but I think saran wrap is not much cheapier.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: FriedEgg]
    #23952020 - 12/23/16 03:56 PM (7 years, 1 month ago)

Quote:

FriedEgg said:
anyone else use "press n seal" wrap instead of standard saran wrap for agar plates?



https://www.amazon.com/Glad-Pressn-Seal-Wrap-Square/dp/B0014D5NVS



I tried it  but prefer regular gad wrap.
Fuck pera film. It's more expensive and dries out over time.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: MudaFuka]
    #23952025 - 12/23/16 03:59 PM (7 years, 1 month ago)

Do you think that for "long time" storage of agar plates in the refrigerator I should better use saran?

At the moment I use a double wrap of para film and use plates with a lot of agar poured into them)

"Long time" = 1-3 months.



I would also use saran wrap for normal plate usage but I have read that only some brands (glad!) allow GE, others don't. Since we don't have Glad in Germany I wasn't able to find out which wrap here in germany allows GE, so I used Parafilm! :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (12/23/16 04:00 PM)


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #23952029 - 12/23/16 04:01 PM (7 years, 1 month ago)

3 months isn't that long. If you gave pera film use it. I just wouldn't buy it when glad wrap is so cheap and works just as well if not better.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineTiamo
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #23952054 - 12/23/16 04:09 PM (7 years, 1 month ago)

My saran wrap was 1 euro for 50 meters. I cut it into 6 mini rolls. I use about 25 cm for one petri, that is 200 petris per mini roll. Or 1200 petris per roll. That's less than a tenth of a cent per petri. :lol:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Tiamo]
    #23952102 - 12/23/16 04:30 PM (7 years, 1 month ago)

Do you guys get a layer of sediment with PDA from potato flakes in no-pour plates? Are potato flakes supposed to dissolve completely in water? I think the instant mashed potatoes we have in my country are not the same most of you guys use. It's a cosmetic thing and the nutes get absorved in the agar puck, but I don't like it. Same thing with BRF agar.
I'll have to try making PDA the traditional way from potato broth to see if this continues to happen.

I made a bunch of MEA no-pour plates the other day for the first time and the LME gets dissolved completely, making the agar puck very pristine and without sediment, I'm loving it so far.


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: Josex]
    #23952125 - 12/23/16 04:37 PM (7 years, 1 month ago)

yeah Josex, in europe we have a slightly different kind of potato flakes. It won't dissolve completely in Agar Agar solution.

I use LME powder and it's really nice and also very cheap. If you don't want to change the media from time to time to give your myc "new food", stay with LME and you'll do fine :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: Josex]
    #23952128 - 12/23/16 04:39 PM (7 years, 1 month ago)

Yeah there's sediment. Have you ever seen a potato completely dissolve in water.:lmafo:


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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InvisibleJosex
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #23952154 - 12/23/16 04:47 PM (7 years, 1 month ago)

Meh.. don't like it :rofl: Have you tried making it with potato broth? any sediment?


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: Josex]
    #23952162 - 12/23/16 04:48 PM (7 years, 1 month ago)

What's your issue with sediment? It doesn't hurt anything.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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