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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: CactiChris]
#26610882 - 04/18/20 03:45 PM (3 years, 9 months ago) |
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Coo coo . Thanks
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 10 hours, 8 minutes
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If you have room in your fridge to spare I'd put just about every plate that was transferred from back into the fridge. Never hurts to have an extra plate to start new grows with!
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: Tweeq] 2
#26610901 - 04/18/20 03:56 PM (3 years, 9 months ago) |
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Thatβs how I do it.

Kinda a pain in the ass on windy days though.
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LAGM2020     
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: Inthepit]
#26611136 - 04/18/20 05:45 PM (3 years, 9 months ago) |
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Quote:
Inthepit said: Pardon, but what do people use to get culture out of the slant? 
1. Scalpel 2. Loop 3. Tweezer thanks, sorry for the interruption...
Loop
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" β Friedrich Nietzsche
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: Cultivation General Discussion [Re: c10h12n2o]
#26611213 - 04/18/20 06:29 PM (3 years, 9 months ago) |
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C10 ya got so much going on, I don't see how you could tear yourself away!
Ya know, I think my loops are too whimpy, small gauge, The loop bends so easily. I'm not heavy handed... So the wire is .010" by my calipers. Is that the norm? 30 AWG
Edited by Inthepit (04/18/20 06:46 PM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: Inthepit]
#26611276 - 04/18/20 07:03 PM (3 years, 9 months ago) |
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Lol yeah I'm a workaholic , workin w 28 varieties currently, not counting edibles or medicinals
My loop is actually from a lab supply store, pretty heavy duty
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" β Friedrich Nietzsche
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: Inthepit]
#26611282 - 04/18/20 07:05 PM (3 years, 9 months ago) |
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Also for C10: I tried your cheese cloth trick with the agar. Definitely cleans up the LME agar. Plates look crystal clear.
Thanks buddy!
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: A.k.a]
#26611369 - 04/18/20 07:38 PM (3 years, 9 months ago) |
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Quote:
A.k.a said: Thatβs how I do it.

Kinda a pain in the ass on windy days though.
You could probably get away with just closing the door. I think what nate said about the flame not producing co2 is correct.
I was thinking about getting a co2 meter just in case but my plan was just to run it in the garage with my doors closed cause theres way more space, lol. I have room there for a AA 941 and brew kettle
If its windy does it fuck with the flame ?
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
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Re: Cultivation General Discussion [Re: sh4d0ws] 1
#26611408 - 04/18/20 07:55 PM (3 years, 9 months ago) |
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So where I was coming from earlier has to do with running propane heaters, which generally put of Carbon Monoxide (CO) and that you do need to be wary of as it can build up and displace the oxygen in the room. Although if you have a big enough room it's ok.
Propane burners and stoves generally have plenty of oxygen and burn cleaner producing CO2 and H20.
Co2 poisoning is essentially just a lack of oxygen if there is enough Co2 to displace it.
Co on the other hand can actually replace Oxygen in the blood an that becomes a bigger problem.
Opening the garage like that serves multiple purposes, firstly if you have a fuel leak you'd rather have it escape rather than build up (though you would likely smell it first that's what the additives are for) and secondly it prevents Co or Co2 buildup from being a problem.
The fact is that you can get away with keeping the door closed in most cases provided you have a big enough area.
You just want to be careful. I had a bud that kept a propane heater going (again different scenario from the burner) on his boat while he was sleeping, CO settled and he woke so tired and oxygen deprived that he barely made it out, said he felt paralyzed.
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: RyeHumor]
#26611429 - 04/18/20 08:05 PM (3 years, 9 months ago) |
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Well my door just had this little weather stripping panel thing on the bottom that broke off so thereβs always like two inches of space.
Yeah if itβs really windy itβll make the flame strength jump all over. Itβs rarely that windy but when it was last week I just moved the trash cans close to it and it was fine.
How much lme are you guys using that youβre straining it?? Iβve never noticed anything more than an occasional string looking thing in the agar.
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LAGM2020     
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: A.k.a]
#26611475 - 04/18/20 08:36 PM (3 years, 9 months ago) |
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I don't strain my LME but never really noticed any issues that would make me want to do it
been using 7.5 grams LME and 10 grams agar to 500mL
It's always mad clear, unless I cook it too long. I also always bring it to a boil first before putting it in media bottles, stirring it really well and getting all the LME to mix in the solution

If I over cook it (this isn't too bad, but some coagulated chunks/specks)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: A.k.a]
#26611576 - 04/18/20 09:33 PM (3 years, 9 months ago) |
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Quote:
A.k.a said: Well my door just had this little weather stripping panel thing on the bottom that broke off so thereβs always like two inches of space.
Yeah if itβs really windy itβll make the flame strength jump all over. Itβs rarely that windy but when it was last week I just moved the trash cans close to it and it was fine.
How much lme are you guys using that youβre straining it?? Iβve never noticed anything more than an occasional string looking thing in the agar.
8 grams in 500mLs it's not about the amount of one though for me it's a pickiness thing and attention to the finer details. I like to use distilled water and I filter through coffee filters before adding agar powder. I dissolve into a small amount of water then filter it three times before topping off to 500ml. It's not necessary in the least but when I do decide to be extra picky plates come out crystal clear. I see so many plates posted that almost drive me crazy lmao. Sometimes I'll just say fuck it and be lazy. sometimes I'll use regular ole tap water, but for the life of me I will never understand how bad I've seen some plates look lol.
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Edited by gizmo1 (04/18/20 09:37 PM)
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MethodicalMystical
Just a normal living being



Registered: 07/16/17
Posts: 247
Loc: PNW
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Re: Cultivation General Discussion [Re: gizmo1]
#26611726 - 04/18/20 10:50 PM (3 years, 9 months ago) |
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Oh dear, I hope my newbish BRF agar plates don't give you too much of a headache
Did my first agar to agar transfers almost a week ago from 3 of 5 germination plates (no growth from 2, slower growth on 5) and want to get some thoughts on what's growing so far.
  
I know I need to work on cleaner cuts. Otherwise... how's it looking? Anything ready for another transfer? Anything worth saving from the germ plates?
-------------------- I know I'm a fool, but that's why I keep trying to learn.
 
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jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
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Quote:
MethodicalMystical said: Oh dear, I hope my newbish BRF agar plates don't give you too much of a headache
Did my first agar to agar transfers almost a week ago from 3 of 5 germination plates (no growth from 2, slower growth on 5) and want to get some thoughts on what's growing so far.
  
I know I need to work on cleaner cuts. Otherwise... how's it looking? Anything ready for another transfer? Anything worth saving from the germ plates?
They all look fine. A better medium will allow you to determine that yourself though
Or just look for the kind of growth you just posted on BRF agar. all looks good to me.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
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Hard to tell from those photos. Not that I'm all that experienced.
But one of the reasons that clear dishes are preferable is that you have an easier time seeing contamination.
Bacteria and satellite growth become really obvious once they are backlit on a clear plate.
From your pictures it's hard to tell if images are hazy or there is condensation.
If you want advice on them, can you post straight on shots of them, backlit if you think it makes them more visible?
Might help people help you.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
MethodicalMystical said: Oh dear, I hope my newbish BRF agar plates don't give you too much of a headache
Did my first agar to agar transfers almost a week ago from 3 of 5 germination plates (no growth from 2, slower growth on 5) and want to get some thoughts on what's growing so far.
  
I know I need to work on cleaner cuts. Otherwise... how's it looking? Anything ready for another transfer? Anything worth saving from the germ plates?
No never your agar doesn't look half bad besides don't listen to me I'm drinking tonight lmao. but... some of the mucellium on them on the other hand doesn't look too great. Cam 3 I'd transfer from that rhizo colony at about 2:30 maybe 3 o'clock looks like good healthy cube mycelium. The other stuff looks awfully thin and I see what looks like maybe bacteria in a couple spots.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: gizmo1]
#26611763 - 04/18/20 11:20 PM (3 years, 9 months ago) |
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cam 1a has some pretty promising looking growth also.
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MethodicalMystical
Just a normal living being



Registered: 07/16/17
Posts: 247
Loc: PNW
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Re: Cultivation General Discussion [Re: RyeHumor]
#26611809 - 04/18/20 11:57 PM (3 years, 9 months ago) |
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There is condensation on some dishes (mostly the germ plates). The pics were taken yesterday and unfortunately, since it was a humid day today, some are even more obscured. Here's the ones I could snap today to the best of my phone's ability. Still doesn't seem to do the details seen by bare eye justice.
   
Quote:
They all look fine. A better medium will allow you to determine that yourself though 
Will keep in mind when my eye is better-trained and I can buy agar again with successful filtering (if needed). I assumed it was looking pretty good (even considered the 4:30 - 5 o'clock area on 1a for a T2) but I didn't want to try without asking and end up taking a contaminant that someone else could have easily recognized.
Quote:
Cam 3 I'd transfer from that rhizo colony at about 2:30 maybe 3 o'clock looks like good healthy cube mycelium. The other stuff looks awfully thin and I see what looks like maybe bacteria in a couple spots.
Yeah, that one is all over the place. Pretty patchy-looking. Couldn't snap it today because of mad condensation but I could probably manage getting the rhizo patch
-------------------- I know I'm a fool, but that's why I keep trying to learn.
 
I also have some 3-year Cambodian prints to get rid of. DM me if interested
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,367
Loc: The Inexpressible...
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Prepping to make my first "clean" spore syringe. Wish me luck giuze!
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
MethodicalMystical said: There is condensation on some dishes (mostly the germ plates). The pics were taken yesterday and unfortunately, since it was a humid day today, some are even more obscured. Here's the ones I could snap today to the best of my phone's ability. Still doesn't seem to do the details seen by bare eye justice.
   
Quote:
They all look fine. A better medium will allow you to determine that yourself though 
Will keep in mind when my eye is better-trained and I can buy agar again with successful filtering (if needed). I assumed it was looking pretty good (even considered the 4:30 - 5 o'clock area on 1a for a T2) but I didn't want to try without asking and end up taking a contaminant that someone else could have easily recognized.
Quote:
Cam 3 I'd transfer from that rhizo colony at about 2:30 maybe 3 o'clock looks like good healthy cube mycelium. The other stuff looks awfully thin and I see what looks like maybe bacteria in a couple spots.
Yeah, that one is all over the place. Pretty patchy-looking. Couldn't snap it today because of mad condensation but I could probably manage getting the rhizo patch
5 o'clock looks like a good spot on the 1a. 5oclock on the new pic not the old one.
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