|
Thrill
Regnarts




Registered: 02/05/11
Posts: 1,928
Loc: Beyond the Grey Sky
Last seen: 3 years, 4 months
|
Re: Cultivation General Discussion [Re: Inthepit]
#26610322 - 04/18/20 12:03 PM (3 years, 9 months ago) |
|
|
Thats a culture slant. Ive never seen any company ship LC in a culture tube before, usually LCs are shipped in syringes like spore solution. Its just some agar poured in there with the wood stick and cooled at an angle to up the surface area, then its inoculated with a little piece of a agar culture.
Use it just like you would a culture on an agar plate. Cut a little piece of it out and drop it on a plate. Master slants can be stored in the fridge for years and still be viable. Easiest way to store cultures long term.
|
poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
|
Re: Cultivation General Discussion [Re: Thrill]
#26610372 - 04/18/20 12:16 PM (3 years, 9 months ago) |
|
|
The issue is that it's hard to access the insides with the scalpel. And all that myc is really thick. I'm always having issues picking up myc from tubes.
|
RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
|
Re: Cultivation General Discussion [Re: Thrill]
#26610379 - 04/18/20 12:17 PM (3 years, 9 months ago) |
|
|
So I guess that once you have a slant or plate fully colonized it's not as likely to get contaminated right?
So you still want to be careful with it, especially you want your transfer to be clean, but its not that likely to loose your master.
Or is it?
|
poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
|
Re: Cultivation General Discussion [Re: RyeHumor]
#26610398 - 04/18/20 12:22 PM (3 years, 9 months ago) |
|
|
I've managed to contaminate my tubes.
Generally, the idea is to create a new tube after you get some nice growth on your petri dish. Slants are for long term storage. For mid term culture work, keep some pertis in the fridge.
|
RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
|
Re: Cultivation General Discussion [Re: poisoned]
#26610423 - 04/18/20 12:26 PM (3 years, 9 months ago) |
|
|
Ok makes sense.
I just brought my old minifridge back from storage expressly for culture storage.
Thing is it must have been a bit damp when I put it away so it smells a bit musty.
Definitely going to bleach bomb it, however there are plenty of porous surfaces (the insulation under the plastic lining) and nooks and crannies.
What do y'all think is the best way to prep it? Or would it be best not to use for fear of contamination?
I expect it should be ok as long as I make sure that the plates/tubes are properly wrapped with cling/parafilm and that I sanitize the surfaces before doing any work with em.
|
Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
|
Re: Cultivation General Discussion [Re: Thrill]
#26610443 - 04/18/20 12:32 PM (3 years, 9 months ago) |
|
|
Quote:
Thrill said: Thats a culture slant. Ive never seen any company ship LC in a culture tube before, usually LCs are shipped in syringes like spore solution. Its just some agar poured in there with the wood stick and cooled at an angle to up the surface area, then its inoculated with a little piece of a agar culture.
Use it just like you would a culture on an agar plate. Cut a little piece of it out and drop it on a plate. Master slants can be stored in the fridge for years and still be viable. Easiest way to store cultures long term.
Awsome replies guys and drum roll from the vendor: Hi,
I used one of our LC labels by mistake instead of the culture label, sorry for the confusion.
The tubes size is Borosilicate Glass Test Tubes w/ Screw Cap 20 x 125 mm - 3/4 x 5" - US Made
ta da, happy trails mush heads!
|
poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
|
Re: Cultivation General Discussion [Re: RyeHumor]
#26610444 - 04/18/20 12:32 PM (3 years, 9 months ago) |
|
|
Just fucked up 80+ glass dishes because I was impatient. Moved them while they were still cooling down, agar went all over the edges.
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Cultivation General Discussion [Re: poisoned]
#26610449 - 04/18/20 12:36 PM (3 years, 9 months ago) |
|
|
Quote:
poisoned said: I've managed to contaminate my tubes.
Generally, the idea is to create a new tube after you get some nice growth on your petri dish. Slants are for long term storage. For mid term culture work, keep some pertis in the fridge.
If you can avoid contaminating the original slant it's best to keep it as the master you want the culture to remain as young as possible so you would want to make a slave plate from the master then use the slave plate to make innoculant plates for as long as possible. Once the slave plate runs out you would go back to the master and make another slave. so you would then have a damn near lifetime supply of a young culture from a single master. If you are contaminating you're masters you need to look into either getting bigger tubes, your sterile Tek, or a different tool to pull from the slant or maybe even a combination of the 3.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Cultivation General Discussion [Re: poisoned]
#26610454 - 04/18/20 12:38 PM (3 years, 9 months ago) |
|
|
Quote:
poisoned said: Just fucked up 80+ glass dishes because I was impatient. Moved them while they were still cooling down, agar went all over the edges.
That sucks man I know I've knocked over my fair share of fresh poured stacks it always sucks when it happens.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
|
Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
|
Re: Cultivation General Discussion [Re: gizmo1]
#26610478 - 04/18/20 12:49 PM (3 years, 9 months ago) |
|
|
Quote:
gizmo1 said:
Quote:
poisoned said: I've managed to contaminate my tubes.
Generally, the idea is to create a new tube after you get some nice growth on your petri dish. Slants are for long term storage. For mid term culture work, keep some pertis in the fridge.
If you can avoid contaminating the original slant it's best to keep it as the master you want the culture to remain as young as possible so you would want to make a slave plate from the master then use the slave plate to make innoculant plates for as long as possible. Once the slave plate runs out you would go back to the master and make another slave. so you would then have a damn near lifetime supply of a young culture from a single master. If you are contaminating you're masters you need to look into either getting bigger tubes, your sterile Tek, or a different tool to pull from the slant or maybe even a combination of the 3.
So the slave plate also goes back the fridge?
Quote:
gizmo1 said:
Quote:
poisoned said: Just fucked up 80+ glass dishes because I was impatient. Moved them while they were still cooling down, agar went all over the edges.
That sucks man I know I've knocked over my fair share of fresh poured stacks it always sucks when it happens.
I burnt my thumb taking plates out the oven too soon, couldn't wait doncha know.
That sucks spilling all that!
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Cultivation General Discussion [Re: Inthepit]
#26610483 - 04/18/20 12:51 PM (3 years, 9 months ago) |
|
|
Quote:
Inthepit said:
Quote:
gizmo1 said:
Quote:
poisoned said: I've managed to contaminate my tubes.
Generally, the idea is to create a new tube after you get some nice growth on your petri dish. Slants are for long term storage. For mid term culture work, keep some pertis in the fridge.
If you can avoid contaminating the original slant it's best to keep it as the master you want the culture to remain as young as possible so you would want to make a slave plate from the master then use the slave plate to make innoculant plates for as long as possible. Once the slave plate runs out you would go back to the master and make another slave. so you would then have a damn near lifetime supply of a young culture from a single master. If you are contaminating you're masters you need to look into either getting bigger tubes, your sterile Tek, or a different tool to pull from the slant or maybe even a combination of the 3.
So the slave plate also goes back the fridge?
Quote:
gizmo1 said:
Quote:
poisoned said: Just fucked up 80+ glass dishes because I was impatient. Moved them while they were still cooling down, agar went all over the edges.
That sucks man I know I've knocked over my fair share of fresh poured stacks it always sucks when it happens.
I burnt my thumb taking plates out the oven too soon, couldn't wait doncha know.
That sucks spilling all that!

Yes once colonized the slave plate goes into the fridge.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
|
Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
|
Re: Cultivation General Discussion [Re: gizmo1] 1
#26610627 - 04/18/20 01:54 PM (3 years, 9 months ago) |
|
|
Dang, the mycilium sticks to the blade. I had to drag it thru the agar and mess it up. The myc itself was nice and fluffy, right at the entrance. It was like scooping ice cream out.
|
Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
|
Re: Cultivation General Discussion [Re: Inthepit]
#26610699 - 04/18/20 02:27 PM (3 years, 9 months ago) |
|
|
Help me out ASAP need to know if I can safely run my 22qt mirro on a propane burner in garage.
|
RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
|
|
as long as you have ventilation and nothing else flammable around it should be ok. Add a fan if you can.
Garage door wide open would probably be best, or at least cracked a foot or two.
Lastly it would be good to have one of those Carbon Monoxide detectors. If you have good ventilation it should be ok but those sensors are pretty cheap insurance.
Also its good practice to hook up your system and spray some soapy water over the connections/lines to make sure you don't have any leaks.
I hardly ever worry about it, but it's one of those things were it could go very wrong...
so check and do all of the above if you are unsure.
Just to be clear you need the garage door to be open to the outside not your house. Ideally you'd have a cross draft going ie open two doors or at least a door and a window.
Edited by RyeHumor (04/18/20 02:41 PM)
|
Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
|
Re: Cultivation General Discussion [Re: RyeHumor]
#26610737 - 04/18/20 02:41 PM (3 years, 9 months ago) |
|
|
Bet, thanks for the info bro 🤟
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
|
How is using a propane burner in a garage any more dangerous than using a propane stove in your kitchen? It isn't. I wouldn't bother with the CM meter.
Any appliance made for flame propane combustion will produce an ideal burn that won't 0roduce carbon monoxide. Propane heaters on the other hand do not produce clean burns so shouldn't be used without ventilation.
--------------------
|
RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
|
Re: Cultivation General Discussion [Re: natedawgnow] 1
#26610809 - 04/18/20 03:16 PM (3 years, 9 months ago) |
|
|
Yeah that's why I said I don't often bother with any of the above.
BUT, if you're asking if it's safe those are all things you COULD do to ensure that things are as safe as they can be.
I think it's worth letting people know if they are specifically asking about safety 
Definitely check the line for leaks if it's not something you've used before, or if it's been a while.
|
CactiChris
Persistently Trying.



Registered: 02/18/18
Posts: 297
Loc: Canada
Last seen: 2 months, 25 days
|
Re: Cultivation General Discussion [Re: natedawgnow]
#26610820 - 04/18/20 03:20 PM (3 years, 9 months ago) |
|
|
Have a window open/ cracked you should be fine. The biggest thing I would worry about is the BTU rating of the stove unit. They tend to be above the limit for pressure canners. To many BTU*s can cause a soft spot in the pot well it is under pressure , Causing a blowout. I believe my presto is 12,000 BTU limit. Most are around that
-------------------- Trade List
|
Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
|
Re: Cultivation General Discussion [Re: CactiChris]
#26610849 - 04/18/20 03:30 PM (3 years, 9 months ago) |
|
|
Yeah I’m shur mine is at the 12,000 btu limit also but do you think it would actually cause a blowout if I ran it on low ? I’m using a granite ware 16 qt and a mirro 22 qt
Edited by Culliganman360 (04/18/20 03:32 PM)
|
CactiChris
Persistently Trying.



Registered: 02/18/18
Posts: 297
Loc: Canada
Last seen: 2 months, 25 days
|
|
Low should be good. It will say somewhere on your burner what it runs at. Or just Google the make and model.
-------------------- Trade List
|
|