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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Sockadin]
    #26566702 - 03/30/20 07:48 AM (3 years, 9 months ago)

Wait so the atl going around came back IDd as tamps?

Or did I misunderstand that?


So they’re like different varieties kinda?

I always wondered about truffles cuz you see soooo many kinds for sale but like nobody grows/breeds them.


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Invisibleforagedfungus
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Re: Cultivation General Discussion [Re: Camera93]
    #26566722 - 03/30/20 08:12 AM (3 years, 9 months ago)

Quote:

Camera93 said:
Anyone have any hotplate recommendations?

I am looking at these 2 based of my search results here

Option 1



Option 2






Can't say about the one with the solid element. But the csr-3t with the coil element can support a fully loaded AA941.


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OfflineSockadin
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Re: Cultivation General Discussion [Re: foragedfungus]
    #26566737 - 03/30/20 08:24 AM (3 years, 9 months ago)

Yes I use option 2 with a loaded PC I do about 8 jars at a time and I finally had the little foot under the coil bend because I was being. A tad drunk one night and tried slamming the lid off the PC. You know when it cools but the kids still kinda stick and your trying to take it off.
Option 2 has worked well for me for years.


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: Sockadin]
    #26566897 - 03/30/20 09:55 AM (3 years, 9 months ago)

Yea option one says it can support up to 12 quart pot I'd go with option 2


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Edited by gizmo1 (03/30/20 09:56 AM)


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Invisiblesh4d0ws
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Re: Cultivation General Discussion [Re: gizmo1]
    #26566955 - 03/30/20 10:34 AM (3 years, 9 months ago)

so I bought the AA 941 on sale here in Canada

Are these the only parts I need to change the jiggle weight out for stopcock??

Quote:

All American Sterilizer #65 Control Valve



+
Quote:

All American Sterilizer 1010 Over Pressure Plug




from here - http://www.allamericancanner.com/All-American-Sterlizer-Parts.htm


Anywhere I can find them cheaper or those prices decent??

Thanks :super:


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #26566996 - 03/30/20 10:58 AM (3 years, 9 months ago)

Do you have a link for the 941?


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: A.k.a]
    #26567030 - 03/30/20 11:16 AM (3 years, 9 months ago)

Quote:

A.k.a said:
Wait so the atl going around came back IDd as tamps?

Or did I misunderstand that?


So they’re like different varieties kinda?

I always wondered about truffles cuz you see soooo many kinds for sale but like nobody grows/breeds them.



From my understanding ATL has always been a misnomer. There was some confusion with the galindoi taxon because it consistently returns Tampanensis results when sequenced.

I think it all was a misnomer. It’s tampanenis and mexicana. I don’t see enough of a difference between galindoi fruits or stones from regular Tampanensis to say it’s a varietal phenotype situation. That said I’ve never sequenced any of them. Apparently Galindo has been coming back as Tamps 🤷🏻‍♂️ Wouldn’t be a huge surprise.

Also if you’re talking about gourmet truffles for cooking, a lot of people have truffle farms. If your talking about the names of stones in smart shops, a lot of those are either completely misinformed or just made up. That’s what I’ve heard and been led to believe anyways

Also check This out. Says that Tamps are just mexicana found in Tampa Florida. Which sounds like a regional miscommunication on the part of fungal taxonomists. Totally possible.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Camera93]
    #26567310 - 03/30/20 01:18 PM (3 years, 9 months ago)

Quote:

Camera93 said:
Anyone have any hotplate recommendations?

I am looking at these 2 based of my search results here

Option 1



Option 2





the first one is nice and cheaper.  using one right now


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26567344 - 03/30/20 01:35 PM (3 years, 9 months ago)

Quote:

jbgtaa said:
Quote:

A.k.a said:
Wait so the atl going around came back IDd as tamps?

Or did I misunderstand that?


So they’re like different varieties kinda?

I always wondered about truffles cuz you see soooo many kinds for sale but like nobody grows/breeds them.



From my understanding ATL has always been a misnomer. There was some confusion with the galindoi taxon because it consistently returns Tampanensis results when sequenced.

I think it all was a misnomer. It’s tampanenis and mexicana. I don’t see enough of a difference between galindoi fruits or stones from regular Tampanensis to say it’s a varietal phenotype situation. That said I’ve never sequenced any of them. Apparently Galindo has been coming back as Tamps 🤷🏻‍♂️ Wouldn’t be a huge surprise.

Also if you’re talking about gourmet truffles for cooking, a lot of people have truffle farms. If your talking about the names of stones in smart shops, a lot of those are either completely misinformed or just made up. That’s what I’ve heard and been led to believe anyways

Also check This out. Says that Tamps are just mexicana found in Tampa Florida. Which sounds like a regional miscommunication on the part of fungal taxonomists. Totally possible.



yes Alt was what was described as Atlantis but was just sequenced in recent months and came back as tamps. in the past couple years DNA has shown all the truffle producers to come back as either tamps or Mex


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: cronicr]
    #26567351 - 03/30/20 01:37 PM (3 years, 9 months ago)

Oh wow I never knew that.

I figured the smart shops were just throwing random names on them to sell more.

I bet they’re identical for the most part, at least cubes a lot of people put time into breeding crosses or new varieties.


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: A.k.a]
    #26567360 - 03/30/20 01:42 PM (3 years, 9 months ago)

you'll see slight pheno differences in some, first pic in my sig is jalisco but look how thick they are, that culture was consistent for it compared to chicon or the a strain


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It doesn't matter what i think of you...all that matters is clean spawn

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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: cronicr]
    #26567462 - 03/30/20 02:25 PM (3 years, 9 months ago)

Damn I need some actual mexicana to play with

Sounds like everything I've fruited is actually tamp varieties .

Mexicana fruits are so gorgeous, explains why mine never look that way haha


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #26567518 - 03/30/20 02:54 PM (3 years, 9 months ago)

Should i take transfers from the very edge of the mycelium or a little farther inwards towards the wedge. I read that the outer mycelium edges isn't desirable. .??

Also what would i need to produce monokaryotic mycelium?


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26567603 - 03/30/20 03:37 PM (3 years, 9 months ago)

Outer edge is usually where people go for. You can even take a piece right next to the edge that looks empty and it’ll grow. The outside is the youngest myc looking for food so it should be fastest.


I gotta look into more stone producers, waiting on my first prints now.

I figure the ones in your signature were some sort of other exotic, definitely thick compare to the other pics I’ve seen.

What kind of myc is the pic next to it??


--------------------
LAGM2020


Edited by A.k.a (03/30/20 03:40 PM)


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: A.k.a]
    #26567639 - 03/30/20 03:50 PM (3 years, 9 months ago)

Quote:

Ovoidhunter said:
Should i take transfers from the very edge of the mycelium or a little farther inwards towards the wedge. I read that the outer mycelium edges isn't desirable. .??

Also what would i need to produce monokaryotic mycelium?




Outer edge is ideal but before it hits the edge of the plate ideally

Serial dilution, streaking technique (example in my sig), microscope, optionally a micro manipulator (or tiny gauge needle) and patience


Quote:

A.k.a said:


I gotta look into more stone producers, waiting on my first prints now.





I can print these tamps for you if you like. At least a few caps look printable.  Tiny ass caps tho


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #26567703 - 03/30/20 04:25 PM (3 years, 9 months ago)

From all my reading, do you really want monokayriotic cultures? I would assume you can’t test it until you’ve isolated all the way down and then you just have an untested isolate.

My theory is that multiple strains work together to create the kinds of cultures we desire as hobbyists


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Offlinepoisoned
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26567734 - 03/30/20 04:41 PM (3 years, 9 months ago)

Monokaryons aren't even that. They are cultures before they have sex and I'm not sure if they even fruit. However, you can use two monokaryons of different varieties to make a cross.


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: jbgtaa] * 2
    #26567735 - 03/30/20 04:42 PM (3 years, 9 months ago)

Monokaryotic is useful for making crosses

Monokaryotic wont fruit by itself though

Monokaryotic is basically like a sperm or egg, only half the required genetic info

Dikaryotic is like an embryo or person or plant: all the genetic material needed to develop. The result of 2 monokaryotic cultures (like sperm + egg)

As far as I understand:
Multiple strains = MS culture, which is a colony of lots of different genetic individuals, which is why you see sectoring and uneven fruit phenotypes , and sectoring on agar

Isolate = a single strain (whether a fruitable dikaryotic or a cross able monokaryotic). These isolates are much more predictable than ms in terms of potency and phenotype and growth characteristics

Ofc people can still do amazing things with ms cultures, it's just less predictable. Commercial cultures are typically proven isolates and thus predictable

Also you dont isolate a monokaryotic culture down in the the same way you would dikaryotic myc. With monokaryotic you have to START as an isolate, because if it touches another compatible monokaryotic (or even dikaryotic) myc it will become dikaryotic, which is irreversible

Single spores germinate as monokaryotic,  which is why to get monokaryotic isolates you have to do things like serial dilution,  and you need a microscope to verify its lack of clamp connections. Also A monokaryotic culture is typically going to be an isolate, unless it's a mat of incompatible monokaryons

Cron or bod or anyone else, please correct me if I'm misunderstanding any of this.


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (03/30/20 04:43 PM)


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #26567829 - 03/30/20 05:44 PM (3 years, 9 months ago)

Thanks guys, leveled me up on my knowledge ya did :thumbup:


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26567843 - 03/30/20 05:55 PM (3 years, 9 months ago)

Does anybody have any experience with storing plates in the fridge?

Wrapped tight in a ziplock bag inside a container?


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