|
inorganik
Stranger

Registered: 06/09/19
Posts: 27
Last seen: 1 year, 1 month
|
Re: Cultivation General Discussion [Re: A.k.a] 1
#26515290 - 03/03/20 07:13 PM (3 years, 10 months ago) |
|
|
Yeah I used search and saw someone say trich infections before first flush usually means bad spawn so.. I guess ill go thru the effort of spawning these guys anyways I have 2..
Been using a homemade 2' x 3' FP flowhood im thinking I didnt run it for long enough. Using it in a bedroom, the cleanest one in the house
Update: in regard to poster above, ditch lysol as it can kill mycelium and is overkill, bad for ur health. The mist I believe helps particles in the air of SAB have something to attach to
Edited by inorganik (03/03/20 07:17 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
|
Quote:
Randalf the Grey said: I'm curious about your spray\mist technique when returning to work on plates. I'm worried that spraying will stir up contams that are on the floor and push them into the lids of the stacked plates.
Have you tried working dry?
|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
|
Re: Cultivation General Discussion [Re: mushboy]
#26515304 - 03/03/20 07:19 PM (3 years, 10 months ago) |
|
|
Quote:
mushboy said:
Quote:
Randalf the Grey said: I'm curious about your spray\mist technique when returning to work on plates. I'm worried that spraying will stir up contams that are on the floor and push them into the lids of the stacked plates.
Have you tried working dry?
LMAO goddamnit mushboy, I haven't seen you in days and I knew you were gonna show up just to say work dry. Lol! You are a man of knowledgeable means, what's your thoughts on contams being pushed into plates before I start working dry?
Edited: I remember reading your dry SAB write up a while back. I remember that wonky ass arm hole. Your disclaimer made me decide to focus of my SAB technique before working dry, though I sometimes do nowadays, but honestly that is out of sheer laziness when spraying would mean going back down stairs.
Edited by Randalf the Grey (03/03/20 07:27 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
|
It's in the thread
All I got is loose theories tho. Like, if a sab is already loaded.. and has been for hours.. then you spray inside... then wait 20min for the air too settle??
I'd rather skip the part where I spray water.
This petri sat OPEN for 30min plus in my dry sab.
|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
|
Re: Cultivation General Discussion [Re: mushboy]
#26515348 - 03/03/20 07:42 PM (3 years, 10 months ago) |
|
|
Quote:
mushboy said: It's in the thread
All I got is loose theories tho. Like, if a sab is already loaded.. and has been for hours.. then you spray inside... then wait 20min for the air too settle??
I'd rather skip the part where I spray water.
This petri sat OPEN for 30min plus in my dry sab.

Ok, ok....feels...
So here's what I'm thinking....let's combine both ideas and make them both slightly better\worse. I had the same thoughts about the sitting for hours(or days) then spraying and waiting 20mins. Seems like it was ok before spraying. I've left plates unwrapped for weeks in my SAB with no contams. But, my table is unlevel as fuck, and racks slightly warpped so a damp towel puts my mind at ease a bit for any tilting or wobble caused by lifting\placing jars and tools and such. Soooo..... Line the bottom with paper towels over hanging the edge of lid so the tub sits on the towels in the groove and latch lid. When ready for use, instead of spraying, pour some water into groove of lid and BOOM. Dry, still air with a damp cloth floor and no need to wait or disturb contents. Eh? How much do you hate it?
|
AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
|
|
Quote:
Camera93 said:
Quote:
Camera93 said: how many times can I PC grain? I have a few bags of oats that didn't seal, or I fucked up, or something happened and I wanna just put them into jars and PC for most of the grain, it'll be the 2nd time through, but 1 bag I already tried to re-PC once..didn't seal so this will be 3 times with them oats
Search function came up with Ham telling someone to start over with after they had used an LC on the grain already and other thread about bags neither shed the light I was hoping for 
I will PC it again but if it fails the second time I usually toss it. Or at the least mix it into fresh prepped grain at a low rate just to use it but it turns pretty dark after a couple runs and the myc doesnt run as fast on it.
|
PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
|
Re: Cultivation General Discussion [Re: dmppb]
#26515433 - 03/03/20 08:24 PM (3 years, 10 months ago) |
|
|
Quote:
inorganik said:

That is trich in its pristine white stage. Dump those.
Quote:
inorganik said:

Wondering if my trich issues (which ive had for the length of this hobby) are from fruiting environment or my grain cleanness. One of the bags I am going to spawn now is seen here, moisture beads or possibly metabolite excretions?
Looks OK to me but pics are never the real thing.
There's a pattern to trich infestations I've seen more than once. You start growing after a long break, never any trich. Some shows up. More shows up. Once you spot it it might be too late to keep it away, but what seems to work is a thorough cleaning of all the work areas and attention to keeping them that way. It can't take many spores to spread it, but I suspect it's making spores even before it turns green...
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
Edited by PrimalSoup (03/03/20 08:34 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
|
ehhh. sounds too hard
|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
|
Re: Cultivation General Discussion [Re: mushboy]
#26515465 - 03/03/20 08:42 PM (3 years, 10 months ago) |
|
|
Right right.
Edit: I wish I had this thought before my first dumbass reply. Instead of water, use a bleach solution or perhaps a quaternary sanitizer. I usually leave the same paper towels in the bottom for several sessions. If using the above stated method of Wet\Dry SAB Tek, this should reduce the frequency of towel changes. It is not to sanitized your SAB work, just to inhibit growth on the towel between session. For the record, I have never seen any signs of growth on my paper towels when used for multiple sessions. I just change them periodically for peace of mind and to clean out loose bits. I'm going to give this a shot. I may do a side by side or something, but that honestly unlikely.
Edited by Randalf the Grey (03/03/20 08:52 PM)
|
Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 15 minutes
|
|
Quote:
Randalf the Grey said: Right right.
I maybe missing it here, but do you put the sheath for the petris back on? I'm talking about, ya pour and cover. Then it seems like what you're saying you come back and are worried about disturbing the air and getting contam.
|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
|
Re: Cultivation General Discussion [Re: Inthepit]
#26515744 - 03/04/20 02:03 AM (3 years, 10 months ago) |
|
|
Nein, I think. If you mean the plastic packaging the plates come in then no. My SAB is wide, but not very tall. Trying to slide it back over stacks inside doesn't go well. So my concern then is that with the plates unwrapped, spraying into the SAB would create enough current to lift contams from the floor and push them between the plate lid and plate base on the bottom layer or two of plates.
Not that I have noticed an issues with the bottom plates, just a thought that occurred. Kinda along mushboy's thinking, my SAB is in a still air room that no one enters and nothing moves. So if the air is already still, why move it by spraying.
Tried my above idea for this session and can say that 1\2pt of water pour into the lid groove is enough to wet the entire floor of paper towel and keep it wet through a long session.
|
RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
|
|
BRF jars are colonizing all at seemingly very different rates, noc’d with a spore syringe 2/16 and some are 85% lookin and others are still moving but at about 20%. Figuring fruiting the first half after they’ve spent a week at 100%. And leave the slowpokes for another sgfc, sound about right?
Anyhow me being impatient and wanting to start agar/improve sterile tek, I decided hey flow hood would be nice. Go to work and whadya know in the back where things sometimes go to get discarded I see vertical laminar flow hood! ‘‘Twas serendipitous, until someone came out and told me it wasn’t being discarded/ no you can’t just stick that in your truck and leave 😬 but if it’s still there this weekend....
|
footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
|
|
Quote:
Randalf the Grey said: Nein, I think. If you mean the plastic packaging the plates come in then no. My SAB is wide, but not very tall. Trying to slide it back over stacks inside doesn't go well. So my concern then is that with the plates unwrapped, spraying into the SAB would create enough current to lift contams from the floor and push them between the plate lid and plate base on the bottom layer or two of plates.
Not that I have noticed an issues with the bottom plates, just a thought that occurred. Kinda along mushboy's thinking, my SAB is in a still air room that no one enters and nothing moves. So if the air is already still, why move it by spraying.
Tried my above idea for this session and can say that 1\2pt of water pour into the lid groove is enough to wet the entire floor of paper towel and keep it wet through a long session.
Why not just wipe it down instead of spraying?
My normal SAB session starts with removing the arm hole covers, saturating a paper towel in ISO, and wiping down the floor really good. Otherwise dry. I only ever clean the whole thing if I move it. I've accidentally skimmed a swab across the floor and still managed to not get any contams... obviously that's pretty lucky even in a soaking wet SAB, but still.
|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
|
Re: Cultivation General Discussion [Re: footpath]
#26515942 - 03/04/20 06:30 AM (3 years, 10 months ago) |
|
|
Quote:
footpath said:
Quote:
Randalf the Grey said: Nein, I think. If you mean the plastic packaging the plates come in then no. My SAB is wide, but not very tall. Trying to slide it back over stacks inside doesn't go well. So my concern then is that with the plates unwrapped, spraying into the SAB would create enough current to lift contams from the floor and push them between the plate lid and plate base on the bottom layer or two of plates.
Not that I have noticed an issues with the bottom plates, just a thought that occurred. Kinda along mushboy's thinking, my SAB is in a still air room that no one enters and nothing moves. So if the air is already still, why move it by spraying.
Tried my above idea for this session and can say that 1\2pt of water pour into the lid groove is enough to wet the entire floor of paper towel and keep it wet through a long session.
Why not just wipe it down instead of spraying?
My normal SAB session starts with removing the arm hole covers, saturating a paper towel in ISO, and wiping down the floor really good. Otherwise dry. I only ever clean the whole thing if I move it. I've accidentally skimmed a swab across the floor and still managed to not get any contams... obviously that's pretty lucky even in a soaking wet SAB, but still.
This ended up being (and still is) and all night lab session. Helping GF get ready for work then heading back in. Of course I am bias because it was my idea, even though I'm likely not the first, I really like this wet\dry idea. I just set everything back up a few day ago so paper towels are only about 4 or 5 days old, but started with them already in and plates in SAB. I poured water into lid groove and slowly but surely the whole floor was saturated. I have been working in SAB off and on for the like 6 hours or so and they have not dried out. Of course the amount of water used will change working time so I can play with that if it stay wet too long. But yeah, I dig it. I have a lot of agar work coming up so I'll have plenty of chances. Time will tell.


|
Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 15 minutes
|
Re: Cultivation General Discussion [Re: footpath]
#26516133 - 03/04/20 09:00 AM (3 years, 10 months ago) |
|
|
Quote:
footpath said: I've accidentally skimmed a swab across the floor and still managed to not get any contams... obviously that's pretty lucky even in a soaking wet SAB, but still.
Whoa! Back the truck up mister...LOL Does that mean you're not using a raised grate over a damp towel?
Seems like I read marked reductions in contam using towel amd grate.
|
footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
|
Re: Cultivation General Discussion [Re: Inthepit]
#26516161 - 03/04/20 09:22 AM (3 years, 10 months ago) |
|
|
I'll often use blank petri dishes or mason jar lid rings as raised work surfaces, but also just work on the floor. I figure if it's just the bottom of a petri dish touching the surface, what difference does it make? My jar lids don't leave my hands with transfers, any tools I set down get torched again, I don't use partial contents of grains for G2G or agar bottles... any compromise will have to come from above. My contam rates are negligible and almost certainly from moments of sloppy handwork. I do like the grate... I dunno why I've been so lazy about getting one...
|
Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 4 days, 4 hours
|
Re: Cultivation General Discussion [Re: footpath]
#26516274 - 03/04/20 10:47 AM (3 years, 10 months ago) |
|
|
I accidentally knocked my stack of empty petris over last time I poured (had the flu, delirious), set them out in light and room temp for 2 weeks, not a single contam.

edit: I did spray though XD
Edited by Thufir_Hawat (03/04/20 11:14 AM)
|
dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 2 months, 17 days
|
Re: Cultivation General Discussion [Re: Thufir_Hawat]
#26516331 - 03/04/20 11:29 AM (3 years, 10 months ago) |
|
|
so my friend brought these back from europe ; what would be the best way to reproduce them ?
i'm doing plates and getting some tissue from the inside and hopefully get clean cultures for grain going , and then just leave them in the jar and wait for stones , sounds good ?
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
|
MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 8 hours
|
Re: Cultivation General Discussion [Re: dmppb]
#26516363 - 03/04/20 11:49 AM (3 years, 10 months ago) |
|
|
Quote:
clockworkshroom said: If you only had the option to fruit in a garage that gets down to 35f/2c at night would you give up?
Being that it gets that cold, you would need to either use a mylar-insulated grow tent, or build a mini-shack inside the garage and insulate it. Both cases, you'll need a space heater to get temps up, but if you're holding above 60*F, you're in great shape. I use a tent and my space heater on a timer, because my basement is 62-64*. The space heater running 15 min once every 3 hours on a low setting (scale is 0-5, i have it at 1.5) bumps the temps to 71-72*. Perfecto!
Quote:
Randalf the Grey said: LMAO goddamnit mushboy, I haven't seen you in days and I knew you were gonna show up just to say work dry. Lol! You are a man of knowledgeable means, what's your thoughts on contams being pushed into plates before I start working dry?
Quote:
Randalf the Grey said: So my concern then is that with the plates unwrapped, spraying into the SAB would create enough current to lift contams from the floor and push them between the plate lid and plate base on the bottom layer or two of plates.
Obscure pathways means contams cannot enter. https://www.shroomery.org/forums/showflat.php/Number/26368517#26368517 The lid of the petri creates enough of an obscure pathway to prevent contams, and then wrapped in the SAB, means great protection. You've even seen this for yourself, you stated you've left petris unwrapped in your SAB and they didn't contam.
I once left 100 plates unwrapped, in the back of my SAB, with the HVAC blowing directly down over the SAB, for three days and not a single contam. Beyond that, i operate my SAB the same way Mushboy does; dry, no towels, just a tub upside down on a table. My contam rate on agar is just under 1%, and almost all of that is operator error because the contams are satellites towards the center, not edge contams. Spraying inside shouldn't create enough of a current to contam your plates, and the reason why i say that is this, i scoot stacks of clean plates across my table all of the time (in a controlled, reasonably slow manner) and never had any edge contams.
Quote:
Inthepit said: Seems like I read marked reductions in contam using towel amd grate.
It certainly doesn't hurt, but it's not necessary. I work directly on my table and don't have any issues. I liked using a grate in the past, but now i've come to discovery it's unnecessary.
|
sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
|
Re: Cultivation General Discussion [Re: Thufir_Hawat]
#26516372 - 03/04/20 11:59 AM (3 years, 10 months ago) |
|
|
Quote:
Thufir_Hawat said: I accidentally knocked my stack of empty petris over last time I poured (had the flu, delirious), set them out in light and room temp for 2 weeks, not a single contam.

edit: I did spray though XD
I poured 60 plates two nights ago and one of the racks wasn't balanced properly in front of my hood. It was like 3AM when this pile of plates came crashing down into the floor. Probably woke up my fam...
Thank fuck I had wrapped a bunch already and they were solidified. Still lost 10+ fuckin plates lol damnit.
|
|