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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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I have done MSS to grain a few times. Every time it was simply because I had extra grain and no agar wedge or grain spawn to noc them with and always assumed they were a loss until I saw otherwise. I think it's been a little less than 50% success rate. It did seem to preform better with slightly overcooked\burst grains. I hypothesize this is because the spores can get into the softer part of the grain to germ instead of doing so on the outside of the grain but I have no evidence to support this other than experience.
I do not recommend MSS to grain unless you do so as an extra and do not count on it for success. If you just have some extra jar though and wanna give it a shot with nothing to lose, go for it! Of course the goal is for optimal conditions and success, but no harm in playing around a bit as log as you know what to expect.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Quote:
Randalf the Grey said: I have done MSS to grain a few times. Every time it was simply because I had extra grain and no agar wedge or grain spawn to noc them with and always assumed they were a loss until I saw otherwise. I think it's been a little less than 50% success rate. It did seem to preform better with slightly overcooked\burst grains. I hypothesize this is because the spores can get into the softer part of the grain to germ instead of doing so on the outside of the grain but I have no evidence to support this other than experience.
I do not recommend MSS to grain unless you do so as an extra and do not count on it for success. If you just have some extra jar though and wanna give it a shot with nothing to lose, go for it! Of course the goal is for optimal conditions and success, but no harm in playing around a bit as log as you know what to expect.
Thanks so much! I'm totally experimenting with all of these new Tek's at this point. I've got BRF cakes I just noc'd up yesterday so I know those should produce 🤞🏽. I do have a few jars of grain prepped, so I'll see what happens with some of the syringes and maybe use some for Agar.
IYO, do you think it's best to start Agar from fresh mushrooms or syringes?
-------------------- There is a better way. Finding the way — well that's the journey
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Clones are, in my experience, easier to clean and you will be working with a more isolated set of genetics so you can have a better idea of the traits you're working with - MS is pretty wildcard. Syringes/prints take a few more steps.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
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Re: Cultivation General Discussion [Re: footpath]
#26514528 - 03/03/20 12:30 PM (3 years, 10 months ago) |
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for sure
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inorganik
Stranger

Registered: 06/09/19
Posts: 27
Last seen: 1 year, 1 month
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Hey all, how are my boxes looking? A little dry im thinking, looking for any advice. The tub walls are lacking moisture in some spots does this indicate too dry? Thanks!
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dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 2 months, 17 days
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Re: Cultivation General Discussion [Re: inorganik]
#26514628 - 03/03/20 01:39 PM (3 years, 10 months ago) |
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Quote:
inorganik said:


Hey all, how are my boxes looking? A little dry im thinking, looking for any advice. The tub walls are lacking moisture in some spots does this indicate too dry? Thanks!
that looks a lot like trich bro , sorry
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: inorganik]
#26514643 - 03/03/20 01:47 PM (3 years, 10 months ago) |
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Quote:
inorganik said:


Hey all, how are my boxes looking? A little dry im thinking, looking for any advice. The tub walls are lacking moisture in some spots does this indicate too dry? Thanks!
Not necessarily. I'd pay more attention to the tint of the surface coir to give you indications of drying. And i dnt thnk that's Trich, but we'll knw for sure if them bitches turn green.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Inthepit]
#26514664 - 03/03/20 01:55 PM (3 years, 10 months ago) |
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Quote:
Inthepit said: So Today I Learned; * 1 lb of Briess Pilsner LME on Ebay @ 10g per 500mL would be 45 times or 1 cent per plate. * 45 times per 20 stack @ $15 ea. stack would be $680 * 24 Corning Vista plates are $109 * so for 7.27 batches of agar and 20 plates I would break even for buying glass.
And yes I did notice someone said they're a pain to work with, don't stack well, etc. Ok, but I'm not really ever going to bulk up, 24 plates and mebbe a stack plastics is all I need. SIGMA CLS70165101-24EA CORNING(R) VISTA(TM) PETRI DISH DIAM. 100x15mm 
I've been back working with glass since I've had them ever since I bought a couple dozen or so years back (Kimax). The downside is easier contamination since they aren't as close tolerance as plastic, and they break too damn easily. I've lost 4 or 5 and it's always the tops. One slipped out of my iso'd gloves in the SAB a couple weeks ago and landing on its side, cracked it. So now I don't really have enough complete plates but they're about $5 each so that's a quandry.
OTOH work great when used right, last forever IF YOU DON'T BREAK THEM. And no waste to speak of.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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inorganik
Stranger

Registered: 06/09/19
Posts: 27
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26514666 - 03/03/20 01:56 PM (3 years, 10 months ago) |
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They're in a closet which I didnt bother to clean before hand probably why..
I do think its trich now that I see green in another tub, and the white surrounding the green has same consistency. thinking of building a small clean room.
Is there anything I can do to help these possibly trich infested tubs?
Maybe gypsum in the coir will help
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
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Re: Cultivation General Discussion [Re: inorganik]
#26514670 - 03/03/20 01:58 PM (3 years, 10 months ago) |
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Quote:
Camera93 said: how many times can I PC grain? I have a few bags of oats that didn't seal, or I fucked up, or something happened and I wanna just put them into jars and PC for most of the grain, it'll be the 2nd time through, but 1 bag I already tried to re-PC once..didn't seal so this will be 3 times with them oats
Search function came up with Ham telling someone to start over with after they had used an LC on the grain already and other thread about bags neither shed the light I was hoping for
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26514671 - 03/03/20 01:58 PM (3 years, 10 months ago) |
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Quote:
GypsyCurse said:
Quote:
inorganik said:


Hey all, how are my boxes looking? A little dry im thinking, looking for any advice. The tub walls are lacking moisture in some spots does this indicate too dry? Thanks!
Not necessarily. I'd pay more attention to the tint of the surface coir to give you indications of drying. And i dnt thnk that's Trich, but we'll knw for sure if them bitches turn green.
yeap ; check it constantly and do not let it turn green and dump spores on your shit
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26514672 - 03/03/20 01:58 PM (3 years, 10 months ago) |
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My first thought was trich also, but it could be different looking myc.
Moisture level seems ok, normally you don’t start to get a lot of condensation and water beads until the surface is colonized.
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LAGM2020     
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: A.k.a]
#26514739 - 03/03/20 02:38 PM (3 years, 10 months ago) |
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It's been a few years since I read about the difference between regular dried rye and these seeds that have been I guess 'germinated' from the looks of it. I have come cultures that are crawling solo slowly on these and I was so happy about the grain prep as well, boiled until they were really moist and plump and not one burst grain. Never had that happen before 
A friend agave me sone of his grains to try out I said they look weird to me so let's test them. I'm making new gfai. Tonight with the normal looking Rye
-------------------- Cakes inside Water Tub
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Nigel Stephens

Registered: 02/21/20
Posts: 103
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Quote:
Nigel Stephens said:
Quote:
Nigel Stephens said: There are a couple of bright white, spherical, pea sized mycelia-like growths occurring on my nearly fully colonised oat grain jars. They’ve been there a few (5) days with no change in colour. Having not witnessed trich before; is this it? Have I earned my wings 😂
Quote:
mushboy said: Post pics

No change today, they look bacterial to me too now. Sad
Any opinions on tossing this jar friends? Edit: btw the dark specks are coffee
Edited by Nigel Stephens (03/03/20 03:51 PM)
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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I'd toss it. At most, top fruit it (put some hydrated coir on top - poke holes in a sandwich bag - put sandwich bag on top of jar). But I kind of doubt that would be successful, either.
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Nigel Stephens

Registered: 02/21/20
Posts: 103
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Re: Cultivation General Discussion [Re: footpath]
#26514956 - 03/03/20 04:38 PM (3 years, 10 months ago) |
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Ok thanks for the fun suggestion, will give that a go. For science!
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inorganik
Stranger

Registered: 06/09/19
Posts: 27
Last seen: 1 year, 1 month
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Wondering if my trich issues (which ive had for the length of this hobby) are from fruiting environment or my grain cleanness. One of the bags I am going to spawn now is seen here, moisture beads or possibly metabolite excretions?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 36 minutes
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Re: Cultivation General Discussion [Re: inorganik]
#26515250 - 03/03/20 06:53 PM (3 years, 10 months ago) |
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I’m not the best at spawn yet and haven’t used bags, but it looks bacterial to me. Something about the thick puffiness of it.
I’ve unfortunately been looking at a lot of it in person lately lol.
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LAGM2020     
Edited by A.k.a (03/03/20 06:54 PM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: inorganik]
#26515283 - 03/03/20 07:11 PM (3 years, 10 months ago) |
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Quote:
inorganik said:

Wondering if my trich issues (which ive had for the length of this hobby) are from fruiting environment or my grain cleanness. One of the bags I am going to spawn now is seen here, moisture beads or possibly metabolite excretions?
So the tubs went trich eh? She is a ruthless bitch that sweet lady trich. It turns out to come from spawn most of the time. Until i started venting my PC my grows were hit or miss. Also changed my sab which changed my position which changed airflow which fucked all kinda shit up that took a while to trace. When i see metabolites in my spawn i get really disappointed. It's from bacteria which is a sneeky sneeky motherfucker. Light bacteria wont necessarily stop a grow but it does piss me off to no end.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: Cultivation General Discussion [Re: A.k.a]
#26515286 - 03/03/20 07:12 PM (3 years, 10 months ago) |
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Quick question for my SAB petri people:
Like many, when I pour my plates, I leave them to cool over night in SAB so condensation can dissipate. I'm curious about your spray\mist technique when returning to work on plates. I'm worried that spraying will stir up contams that are on the floor and push them into the lids of the stacked plates. Is this a symptom of overthinking or is there something to be concerned about. I used to used Lysol as my spray because it gave a super fine mist and I also use it around the house and in the air of my still air room, but switched to h2o specifically because I didn't like how forcefully the mist comes out of a Lysol can. Currently my h2o spray bottle is busy so I had to use Lysol again which is what bought me to ask this question.
Thoughts\comments\concerns\questions\input\insight?
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