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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: dmppb]
#26502401 - 02/24/20 10:25 PM (3 years, 10 months ago) |
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Jump your cycle up to 2 hours, vent for 10. My cycle sits at around 18 psi.
Set the 10 min timer when the steam is heavy and stable, not when it's all sputtery. Should help a ton.
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: dmppb]
#26502404 - 02/24/20 10:26 PM (3 years, 10 months ago) |
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Clean spawn.
You can also tape/glue a couple quarters to your weight to get the PC to maintain 17-18psi. I'll usually run that for 90m.
Have you kept an uninoculated jar around as a control? My guess is that it's coming from your culture. Mold/bacteria can really hide itself.
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jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
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Re: Cultivation General Discussion [Re: footpath]
#26502412 - 02/24/20 10:39 PM (3 years, 10 months ago) |
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Quote:
footpath said: Clean spawn.
You can also tape/glue a couple quarters to your weight to get the PC to maintain 17-18psi. I'll usually run that for 90m.
Have you kept an uninoculated jar around as a control? My guess is that it's coming from your culture. Mold/bacteria can really hide itself.
This. If I were you I would sterilize 10 jars of grain, after venting for 10 min, for two hours. Once sterilized, do what you want with them but leave 2 on your shelf uninoculated and see if they contam. If they do, it’s your filter’s fault. If they don’t, that means your agar cultures aren’t clean enough. Good luck!
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: jbgtaa]
#26502429 - 02/24/20 11:01 PM (3 years, 10 months ago) |
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Quote:
jbgtaa said:
Quote:
footpath said: Clean spawn.
You can also tape/glue a couple quarters to your weight to get the PC to maintain 17-18psi. I'll usually run that for 90m.
Have you kept an uninoculated jar around as a control? My guess is that it's coming from your culture. Mold/bacteria can really hide itself.
This. If I were you I would sterilize 10 jars of grain, after venting for 10 min, for two hours. Once sterilized, do what you want with them but leave 2 on your shelf uninoculated and see if they contam. If they do, it’s your filter’s fault. If they don’t, that means your agar cultures aren’t clean enough. Good luck!
If they do that means it's either filter, lid, OR sterilization cycle(as he suspects) If the don't that means it's either culture OR sterile Tek. I'm probably missing some other unforseen circumstances. It's not as clear cut as you made it seem but good advice none the less. If you are having a contam outbreak use controls to troubleshoot.
And for any one who is going to suggest it's not sterilization because molds die at low temps remember he said he is having a bacteria problem. Bacteria allows other contams to get a foothold.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26502467 - 02/25/20 12:19 AM (3 years, 10 months ago) |
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Quote:
p9hu7 said: Jump your cycle up to 2 hours, vent for 10. My cycle sits at around 18 psi.
Set the 10 min timer when the steam is heavy and stable, not when it's all sputtery. Should help a ton.
AMEN!
and 2hr @ 20PSI @sea level every time for any grain situation at my house;)
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: TedsDead]
#26502495 - 02/25/20 01:14 AM (3 years, 10 months ago) |
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I recently cut my time back from 2 hours to 1.5. Idk how its working out for me so far but I used to get away with 60min on wbs. Idk. If it turns out shit imma be pissed I used a entire plate on the jars from a culture that for some reason wasn't backed up. Used most of the plate then accidently dropped the remainder before I got a chance to transfer to another plate. So if I show up pissed one day you guys know why.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: gizmo1]
#26502496 - 02/25/20 01:16 AM (3 years, 10 months ago) |
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wbs is mostly milo which has a small volume. the smaller the grain the more u can get away with it. thats why u can steam BRF in 90 min
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 10 hours, 32 minutes
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Re: Cultivation General Discussion [Re: TedsDead]
#26502947 - 02/25/20 11:02 AM (3 years, 10 months ago) |
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Some questions on cloning:
- When you clone, grow, print, grow, clone, grow, print ect. Are you stabilizing genetics from spore? So you lose the diversity of multispore genetics?
- Is testing clones in shoeboxes a good method to determine which to keep to scale up to monos? I seen Stare recommends testing in whatever FC you plan to fruit in in permanently
- What does your clone program look like?
I have a lot of varieties and my OCD says I want to have a saved culture from all of them that I can grow out at anytime with predictable results [*1] MS -> 3-4 clones taken [*2] each clone grown out in a shoebox [*3] Best performer is kept and scaled to run in monos
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Nigel Stephens

Registered: 02/21/20
Posts: 103
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Re: Cultivation General Discussion [Re: Camera93]
#26503108 - 02/25/20 12:38 PM (3 years, 10 months ago) |
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Do I have anything to worry about here, are they safe to eat? I think it’s pseudomonas or verticillium, stems are hollow too
This is 2nd flush after dunking substrate / floating harvest tek. Have since removed lid for FAE and drying but should I just harvest and toss? Obvs then bleach my whole grow area 
Thank-you in advance for your time and advice


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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 4 hours, 25 minutes
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Nothing wrong with them, eat em.
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dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 2 months, 17 days
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Gracias a bunch fam ,
how much water you put in there so it can lasts 2 hours , same 3 quarts ? i just bought new gaskets for my prestos so no loose ends on the process
i will increase the cycle to 2 hours , and glue some coins to the steam lock to keep it at least at 17 psi ; fell kinda dumb neglecting pc cycle time ; now that i see it , i 'll leave a couple of test jars and confirm
also , my sfd sometimes are soaking wet after the cycle , could this affect ?
@TedsDead isnt' 20 at the warning sign? ha i freak when it goes to 19
about the hidden contams, this scares me the most , i'm thinking about buying a microscope and start really looking , my culures are looking like this :

but then the master jars look like this , ( kinda ok but not so much )
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: TedsDead]
#26503227 - 02/25/20 01:43 PM (3 years, 10 months ago) |
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Quote:
TedsDead said: wbs is mostly milo which has a small volume. the smaller the grain the more u can get away with it. thats why u can steam BRF in 90 min
Yea but this wasn't specific to wbs I was using mostly wbs but also rye and wheat. Plus my wbs isn't all Milo and millet. I mean that's a big part of it but I use the one with cracked corn sunflower seeds and a bunch of other grains. The that was mixed 50/50 with whatever other grain I had on hand at the time. Idk exactly what to attribute my success at the time to, but I doubt it was small grains. I only say this because there were also bigger grains in there. I even remember running popcorn wbs mix for 60 minutes. I wouldnt do it now, but I am trying to cut back my time a little now that I'm not using oats much. 1.5 hours should be fine at least for something with no hull right? Wbs doesn't really have any grains with hulls. The barley that. Using now does but I'm seeing how that goes now. I'll let you guys know if they all fked or if it's fine. I feel like if we aren't using oats no need to go 2 hours though. Maybe we all tend to overcook the shit outta the grains when it's not necessary.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Camera93]
#26503229 - 02/25/20 01:44 PM (3 years, 10 months ago) |
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Quote:
Camera93 said: Some questions on cloning:
When you clone, grow, print, grow, clone, grow, print ect. Are you stabilizing genetics from spore? So you lose the diversity of multispore genetics?
Pretty obviously. That's how varieties get stabilized isn't it?
Quote:
Camera93 said:Is testing clones in shoeboxes a good method to determine which to keep to scale up to monos? I seen Stare recommends testing in whatever FC you plan to fruit in in permanently
Dunno, but I've never done much with clones before but have been recently. It helps that I'm getting better success at cloning, including edibles. 
Using little 1 qt cased grows in a FC gives me the information I'm looking for (pheno, yield, and potency in the opposite order). Unless I was trying to really wring the max out of a culture I don't see how they won't translate to bulk...
I could be wrong of course but my goals are simple - mad crops and easy harvests.
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 3 hours
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Re: Cultivation General Discussion [Re: dmppb]
#26503232 - 02/25/20 01:45 PM (3 years, 10 months ago) |
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Quote:
Air trapped in a pressure canner lowers the temperature and this results in underprocessing. To be safe, all pressure canners must be vented for 10 minutes before they are pressurized. https://www.ag.ndsu.edu/publications/food-nutrition/questions-and-answers-about-using-a-pressure-canner
Quote:
In the case of the venting step, it is needed to ensure that your canner is voided of air entirely. When the water starts to boil, it turns to a gas and this gas mixes with air. If you do it in a sealed container with a small exit (like a canner), the steam can push out the air, but it needs some time to do so. This is why recipes are designed with a vent time at the beginning. Air pockets would interfere with the heating process. Only after you have achieved the canner-full-of-steam-without-air condition, can you start building the pressure. https://cooking.stackexchange.com/questions/103376/why-let-a-pressure-canner-vent-before-putting-the-weight-on
Well I couldn't find the really cool scientific explanation but anyway air doesn't get as hot as steam.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: dmppb]
#26503235 - 02/25/20 01:47 PM (3 years, 10 months ago) |
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Quote:
dmppb said: Gracias a bunch fam ,
how much water you put in there so it can lasts 2 hours , same 3 quarts ? i just bought new gaskets for my prestos so no loose ends on the process
i will increase the cycle to 2 hours , and glue some coins to the steam lock to keep it at least at 17 psi ; fell kinda dumb neglecting pc cycle time ; now that i see it , i 'll leave a couple of test jars and confirm
also , my sfd sometimes are soaking wet after the cycle , could this affect ?
@TedsDead isnt' 20 at the warning sign? ha i freak when it goes to 19
about the hidden contams, this scares me the most , i'm thinking about buying a microscope and start really looking , my culures are looking like this :

but then the master jars look like this , ( kinda ok but not so much )

Yes 3 quarts will be plenty of water. I only use like 2 quarts jars full the maybe a little extra sometimes and a shot of vinegar.
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dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 2 months, 17 days
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Re: Cultivation General Discussion [Re: gizmo1]
#26503266 - 02/25/20 02:07 PM (3 years, 10 months ago) |
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Quote:
gizmo1 said:
Quote:
dmppb said: Gracias a bunch fam ,
how much water you put in there so it can lasts 2 hours , same 3 quarts ? i just bought new gaskets for my prestos so no loose ends on the process
i will increase the cycle to 2 hours , and glue some coins to the steam lock to keep it at least at 17 psi ; fell kinda dumb neglecting pc cycle time ; now that i see it , i 'll leave a couple of test jars and confirm
also , my sfd sometimes are soaking wet after the cycle , could this affect ?
@TedsDead isnt' 20 at the warning sign? ha i freak when it goes to 19
about the hidden contams, this scares me the most , i'm thinking about buying a microscope and start really looking , my culures are looking like this :

but then the master jars look like this , ( kinda ok but not so much )

Yes 3 quarts will be plenty of water. I only use like 2 quarts jars full the maybe a little extra sometimes and a shot of vinegar.
noted ;
i don't use a regular stove , but a bigass potent burner , i can get 5 psi's at the end of the 10 minutes of venting if used at medium , sometimes i have ran out of water mid cycle while using 3 quarts ,
my question is : can venting at high flame make too much water escape so it can run out during the cycle ? or is it my shitty gaskets ? (which i just bought new and will replace old ones in the next cycle)
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
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shroomgirl645
Microdoser extraordinaire 🍄😊🍄



Registered: 12/03/19
Posts: 151
Loc: The Shroom Shack
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: dmppb]
#26503605 - 02/25/20 05:13 PM (3 years, 10 months ago) |
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finally got a couple of pins of the top of my tub. I was worried it was just gonna be sidepins lol
anyways, would it be possible to dump out a fully colonized shoebox to fruit/harvest it?
EDIT: to avoid posting twice:
can I harvest small sidepins without harming anything?
-------------------- New to Shroom Cultivation | Current Status: Tubs 3/5/2020 1&2 (Fruiting)
Edited by shroomgirl645 (02/25/20 05:48 PM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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You can do whatever you like madam, the world is your oyster. I seen sh4d0ws suspend a sub from fires in a monotub. You can let it crap out sub level dongers at will, then when your ready to harvest jst remove the fruit off the top, turn the box over and tap the sub out to harvest the rest.
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shroomgirl645
Microdoser extraordinaire 🍄😊🍄



Registered: 12/03/19
Posts: 151
Loc: The Shroom Shack
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26503783 - 02/25/20 06:29 PM (3 years, 10 months ago) |
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thank you, I have a few side pins that would fit in capsules if I harvest now, so I figured I'd do that since I'm so impatient
-------------------- New to Shroom Cultivation | Current Status: Tubs 3/5/2020 1&2 (Fruiting)
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26503965 - 02/25/20 08:07 PM (3 years, 10 months ago) |
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Quote:
GypsyCurse said: I seen sh4d0ws suspend a sub from fires in a monotub.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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