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OfflineInthepit
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Re: Cultivation General Discussion [Re: AyePlus]
    #26498316 - 02/22/20 11:14 AM (3 years, 11 months ago)

Inthepit said:
Quick question, should do transfers from a pin?
Or is a pin isolated enough already?
   
:shrug:  :hatsoff:

Thankyou for your reply!
AyePlus said:
Did the pin come from a clean plate or was it from the germ plate?
Yes the pin came from the Germ plate.

I generally do At least 1 xfer from TIssue Culture or Pin Clone plate and make a grain jar to test from that, and put the TC/PC plate in cold storage, if the transfer grows out clean and the clone is a winner I’ll slant from the TC/PC plate and make a bunch of work plates.

if it was a germ plate I might still do 2-3 transfers.

Imo just based on looks that one needs another 1-2 to be clean, or your temps are swinging pretty drastically.

The room isn't temp controlled, yer right. Temp is 79-84F.

I'll do more Ts, thanks  :hatsoff:


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OfflineHuskies
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Re: Cultivation General Discussion [Re: Inthepit]
    #26498343 - 02/22/20 11:35 AM (3 years, 11 months ago)

Hey so I bought a BUNCH of gypsum way back, it was from a place that did gypsum from drywall recycling.

My question is, is that likely to be super contaminated with trich and other molds?

Not sure if I should consider using it. I did in the past, but trich happened to be a huge issue back then. I don't remember if I ever narrowed it down to the gypsum.

If its added to jars, would those molds survive 2 hours at 16psi?


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26498348 - 02/22/20 11:38 AM (3 years, 11 months ago)

Quote:

Crackatoa said:
:whathesaid: I've been having that issue of lids being a mofo, I've started flipping mine, seal up. Works good but lid slids easily. I have been wanting to look for tek on getting that seal from the bottom of lids off.




Well shit! It doesn’t leak when you shake?
I might have to try that.. I use upside down lids on my grain jars.

Definitely worth a try. Thanks crack. :rockon:


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26498352 - 02/22/20 11:43 AM (3 years, 11 months ago)

Good point, I use a stir plate and don't have to worry about shaking. I always hated shaking a LC. Def worth the money.


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OfflineOregonic
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26498412 - 02/22/20 12:39 PM (3 years, 11 months ago)

So I just started back up after a long hiatus and it seems I've got that new grower impatience all over again. A bit more than a week since swiping plates and no signs of germ on almost 20 plates - I wanted to start a handful of different cube varieties since most of my spores are a little old, bit over 3 years. I realize these spores should mostly be viable and I just need to chill but I guess I'm just hyped to get going again.

I did a bit of searching and it seems like <5 year old prints should be okay, but should I have hydrated them first to increase my odds?

Anyway, I had half a dozen of PESH oat spawn jars that have been fully colonized for a, uh, long time (couple years), forgotten, sitting in tub in a closet all dried and shrunken and sad. I loved that clone however so I figured wtf and threw a couple old colonized grains on agar.

Is this a futile attempt? I've heard of reviving old mycelium but I'm guessing after sitting at room temps for so long the shit is dead dead. Wish I'd never taken a break, damn.

Anyway hope everyone is having a good weekend! :peace:


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Inthepit] * 2
    #26498463 - 02/22/20 01:06 PM (3 years, 11 months ago)

Quote:

Inthepit said:
Inthepit said:
Quick question, should do transfers from a pin?
Or is a pin isolated enough already?
   
:shrug:  :hatsoff:

Thankyou for your reply!
AyePlus said:
Did the pin come from a clean plate or was it from the germ plate?
Yes the pin came from the Germ plate.

I generally do At least 1 xfer from TIssue Culture or Pin Clone plate and make a grain jar to test from that, and put the TC/PC plate in cold storage, if the transfer grows out clean and the clone is a winner I’ll slant from the TC/PC plate and make a bunch of work plates.

if it was a germ plate I might still do 2-3 transfers.

Imo just based on looks that one needs another 1-2 to be clean, or your temps are swinging pretty drastically.

The room isn't temp controlled, yer right. Temp is 79-84F.

I'll do more Ts, thanks  :hatsoff:




The temp fluctuations give rise to growth rings sometimes, so since this came from a clean plate and looks very regular aside from those rings you probably don't need to do more transfers. IMHO that is. 

But it wouldn't hurt to put one aside in a place that doesn't have temp swings to see how it grows. :shrug:


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #26498776 - 02/22/20 05:11 PM (3 years, 11 months ago)

:whathesaid:

Looks pretty clean to me if your temps are swinging.

I’d make a work plate off that and put it in cold storage, drop the work plate to grain and run a test fruiting, then make a slant off the plate in cold storage and make Another master plate and more work plates if its worth keeping.


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Offlinebongoman
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Re: Cultivation General Discussion [Re: AyePlus]
    #26498953 - 02/22/20 07:28 PM (3 years, 11 months ago)

All my AA+ jars look like the photo on the right and all my Taman Negara jars like the photo on the left.

Both had a 70% shake 3 days ago. Should the difference be of concern?



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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: bongoman]
    #26498958 - 02/22/20 07:30 PM (3 years, 11 months ago)

They both have some bacteria issues the photo on the left is worse.


Both should still fruit. I probably wouldnt expand either, but the farthest right looks best of those 4


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Offlinebongoman
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Re: Cultivation General Discussion [Re: AyePlus]
    #26498964 - 02/22/20 07:36 PM (3 years, 11 months ago)

Thanks, it seems that I can never quite get clean grain spawn. I never have issues with mold but all my jars seem slightly bacterial although my donor plates always seem pristine.

I guess it might be my grain prep? All jars have full SFD’s on them and are PC’d for 2hrs at 15psi.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: bongoman]
    #26498974 - 02/22/20 07:42 PM (3 years, 11 months ago)

Bacteria in grain usually comes from improper sterilization or the cultivator. Maybe you’re not flaming your scalpel enough or touching the rim of the jar or agar or maybe your inoculant isnt as clean as you think? post a picture of your inoculant next time before droping it.

Are you making sure to vent your pressure cooker before bringing up to pressure?

I’ve dealt with bacterial issues with certain grains as well seems like lots of people have trouble with oats whether its bacterial endospores or just bad prep but I’ve also heard of bad batches of other grain types as well.


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Re: Cultivation General Discussion [Re: AyePlus]
    #26499152 - 02/22/20 09:48 PM (3 years, 11 months ago)

I def agree that the left pic doesnt look so well, but dont believe theres enough info to tell about the AA jars esp. without being able to zoom in.  those couple grains up against the glass are concerning but IME everytime I take a pic of even my cleanest jars they always look shitty and the glare off the glass makes em look wet.    if you spawn em to coir and take notes tho your eye will improve and if they trich out on coir it was most likely due to your spawn.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: TedsDead]
    #26499212 - 02/22/20 10:30 PM (3 years, 11 months ago)

I can zoom on my phone, the right one might be pretty decent but theres a spot about halfway up on the left one towards the right side I dont like


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: AyePlus]
    #26499245 - 02/22/20 11:13 PM (3 years, 11 months ago)

agreed... I just mention it at all cause as a noob just listening to people tell u this is good or this is bad can be extremely confusing. I know it was for me.  if they spawn all of them and keep notes tho they'll soon know for themselves and they get to do science, LOL .  learning that the green DID come from your spawn can be a huge hurdle


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26499376 - 02/23/20 03:14 AM (3 years, 11 months ago)

Quote:

Infinity-25 said:
Quote:

Edmunter said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.





Update:

Shook the
Quote:

Smoothcat said:
Personally I would have let it grown out a bit more before shake but that’s just me you would have probably had a quicker recovery time. Also there’s not much growth after a week so that could insinuate that it’s battling something. What are your ambient temps like and what is it you’re growing there?

These are my pesa 3 days after shake






Its cubes clones, incubating in dark mostly in boiler room 20-25C. Plates looked clean. Thought maybe the grains are on the dry side.




IMHO jars that take that long are always fucked.  Ivenever had a jar take 6 weeks and the outcome was anything other than half a flush b4 fucking up.


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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: Edmunter]
    #26499386 - 02/23/20 03:48 AM (3 years, 11 months ago)

I've decided to try to do some MS>agar>g2g in The near future. I'm thinking wild birdseed :thumbup::cool:might be a good option. Where would one find a big bag of bird seed? Is there any specific kind that is better?


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OfflineFun-G
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26499394 - 02/23/20 04:12 AM (3 years, 11 months ago)

Avoid (if you can) brands with shredded corn and peanuts.... they're usually the dirtiest of them all.

I'm using red ribbons currently, it's free of peanuts but the shredded corn sucks....still works though.

I know you can get some brands of wbs free of both shredded corn and peanuts but some will even include gypsum! That's a score whenever I find that brand.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Fun-G]
    #26499549 - 02/23/20 08:25 AM (3 years, 11 months ago)

40# penningtons at Walmart for $15


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Offlinedmppb
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26499582 - 02/23/20 08:47 AM (3 years, 11 months ago)

morning ;

a question about sfd's , you can just wash them with water , and use ?  no need to foil or nothing , right ? doesn't matter if they are wet ?

:heart:


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InvisibleMadHatter333
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Re: Cultivation General Discussion [Re: dmppb]
    #26499585 - 02/23/20 08:50 AM (3 years, 11 months ago)

You honestly don’t need to wash them if you’re running your jars in the PC. You don’t want them to stay wet for to long so foil is recommended still while you PC.


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