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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: jbgtaa]
#26496945 - 02/21/20 12:33 PM (3 years, 11 months ago) |
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LadysKnight said: Agreed, first is mold. jbgtaa said: Inthepit said: Am I isolating contam? This GT looks so weird. I'm out to T6 now and no change. 
 First one is def not cube Myc. Second one just looks like MS culture but also could be something else.
Looks I'm gonna have to give up on GT
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Inthepit] 1
#26497149 - 02/21/20 03:10 PM (3 years, 11 months ago) |
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jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
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Re: Cultivation General Discussion [Re: Inthepit] 1
#26497190 - 02/21/20 03:30 PM (3 years, 11 months ago) |
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Quote:
Inthepit said: LadysKnight said: Agreed, first is mold. jbgtaa said: Inthepit said: Am I isolating contam? This GT looks so weird. I'm out to T6 now and no change. 
 First one is def not cube Myc. Second one just looks like MS culture but also could be something else.
Looks I'm gonna have to give up on GT
Incorrect, just start a few different plate. You most likely introduced that contamination yourself. I would be surprised if you were starting with a mold culture in your syringe or whatever.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26497253 - 02/21/20 04:17 PM (3 years, 11 months ago) |
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Quote:
GypsyCurse said:
Quote:
Inthepit said:



Ha ha, Gypsycurse, you crack me up!
Well, from syringe, noob totally fucked it up. Live and learn eh...
Edited by Inthepit (02/22/20 04:14 AM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: jbgtaa]
#26497264 - 02/21/20 04:25 PM (3 years, 11 months ago) |
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 I dnt knw if you jst bellyaching like fuck GT its got bad mojo now or what, and i personally don't like GT either, but that aint the cause of this shit. Them contaminates can be very difficult to distinguish from cube myc and in many instances it goes undetected until it's melded seamlessly with cube myc. Myco learning curve is a sharp bitch. Takes on average a year of consistent trial & error type work to round it. Dnt he hard on yourself or the variety, jst keep going.
Lol yeah i thnk that gag thing that people do is damn funny.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26497417 - 02/21/20 05:55 PM (3 years, 11 months ago) |
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Thank you for the encouraging words. I'll keep at it. I have a very specific goal.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Inthepit] 1
#26497461 - 02/21/20 06:27 PM (3 years, 11 months ago) |
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Specific goal? You mind sharing?
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26497471 - 02/21/20 06:31 PM (3 years, 11 months ago) |
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Oh sure, I've been meditating for twenty years and before that a seeker from forever. My goal is in laymans terms ego death, I have a barrier. It keeps me from... and I feel shrooms can break down that barrier.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 28 minutes
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Re: Cultivation General Discussion [Re: Inthepit] 1
#26497483 - 02/21/20 06:37 PM (3 years, 11 months ago) |
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Yes they will. I would go for PE or pans
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,368
Loc: The Inexpressible...
Last seen: 5 minutes, 9 seconds
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Re: Cultivation General Discussion [Re: Crackatoa]
#26497484 - 02/21/20 06:38 PM (3 years, 11 months ago) |
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Anyone know where the "cleanest" spot of a spore print is?
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Inthepit] 1
#26497514 - 02/21/20 06:59 PM (3 years, 11 months ago) |
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Quote:
Inthepit said: Oh sure, I've been meditating for twenty years and before that a seeker from forever. My goal is in laymans terms ego death, I have a barrier. It keeps me from... and I feel shrooms can break down that barrier.
Interesting. I practice a slower more additive kinda sober ego death. It's difficult but the practice burns karma.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26497984 - 02/22/20 05:33 AM (3 years, 11 months ago) |
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Well practices: Jhana, Vipassana, Zen, Liberation Unleashed, The Fetters, on and on... Funny, in 1965 I put Buddhism as my religion in my Marine Corp file.
Edited by Inthepit (02/22/20 05:34 AM)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Cultivation General Discussion [Re: Inthepit]
#26498124 - 02/22/20 08:20 AM (3 years, 11 months ago) |
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I just did my first blenderless Li yesterday. Now I’m sitting staring at the jars like..
 DID IT WORK?!?!
-------------------- Tek's I use LAGM2020
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jdawg333
Stranger
Registered: 08/22/18
Posts: 580
Last seen: 19 days, 19 hours
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Can PF Tek be accomplished by steaming narrow mouthed quart sized jars of BRF and verm, placed into a larger container when ready?
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 28 minutes
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Re: Cultivation General Discussion [Re: jdawg333]
#26498152 - 02/22/20 08:45 AM (3 years, 11 months ago) |
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Yes, you'll have to dig it out and you could make a shoebox
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Crackatoa]
#26498156 - 02/22/20 08:50 AM (3 years, 11 months ago) |
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Quick question, should do transfers from a pin? Or is a pin isolated enough already? 
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Infinity-25
Stranger



Registered: 04/07/10
Posts: 129
Last seen: 1 year, 3 months
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Quote:
staytrippy420 said: I just did my first blenderless Li yesterday. Now I’m sitting staring at the jars like..
 DID IT WORK?!?!
Haha I can fully imagine. Plan on doing the same tomorrow ^^
Btw can I make blenderless LI from a half colonized agar plate? Just testing this out so not going to make loads of jars yet and my furthest plate atm is only about 50% colonized. If I just cut the myc part out and put it to the water jar should still work right?
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Cultivation General Discussion [Re: Infinity-25]
#26498178 - 02/22/20 09:10 AM (3 years, 11 months ago) |
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Hell yeah man long as it’s clean and you fallow sterile procedure in the sab you should be good.. the hardest part for me was breaking the seal on my jar of sterilized water. Was a bitch to get off in the sab.
-------------------- Tek's I use LAGM2020
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 5 hours, 28 minutes
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I've been having that issue of lids being a mofo, I've started flipping mine, seal up. Works good but lid slids easily. I have been wanting to look for tek on getting that seal from the bottom of lids off.
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: Inthepit]
#26498266 - 02/22/20 10:16 AM (3 years, 11 months ago) |
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Quote:
Inthepit said: Quick question, should do transfers from a pin? Or is a pin isolated enough already? 

Did the pin come from a clean plate or was it from the germ plate?
I generally do At least 1 xfer from TIssue Culture or Pin Clone plate and make a grain jar to test from that, and put the TC/PC plate in cold storage, if the transfer grows out clean and the clone is a winner I’ll slant from the TC/PC plate and make a bunch of work plates.
if it was a germ plate I might still do 2-3 transfers.
Imo just based on looks that one needs another 1-2 to be clean, or your temps are swinging pretty drastically.
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