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OfflineInthepit
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26496945 - 02/21/20 12:33 PM (3 years, 11 months ago)

LadysKnight said:
Agreed, first is mold.
jbgtaa said:
Inthepit said:
Am I isolating contam? This GT looks so weird.
I'm out to T6 now and no change.
       
:shrug:
First one is def not cube Myc. Second one just looks like MS culture but also could be something else.

Looks I'm gonna have to give up on GT


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26497149 - 02/21/20 03:10 PM (3 years, 11 months ago)

Quote:

Inthepit said:
   
:shrug:





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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26497190 - 02/21/20 03:30 PM (3 years, 11 months ago)

Quote:

Inthepit said:
LadysKnight said:
Agreed, first is mold.
jbgtaa said:
Inthepit said:
Am I isolating contam? This GT looks so weird.
I'm out to T6 now and no change.
       
:shrug:
First one is def not cube Myc. Second one just looks like MS culture but also could be something else.

Looks I'm gonna have to give up on GT



Incorrect, just start a few different plate. You most likely introduced that contamination yourself. I would be surprised if you were starting with a mold culture in your syringe or whatever.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26497253 - 02/21/20 04:17 PM (3 years, 11 months ago)

Quote:

GypsyCurse said:
Quote:

Inthepit said:
   
:shrug:







Ha ha, Gypsycurse, you crack me up!

Well, from syringe, noob totally fucked it up.
Live and learn eh...


Edited by Inthepit (02/22/20 04:14 AM)


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: jbgtaa]
    #26497264 - 02/21/20 04:25 PM (3 years, 11 months ago)

:whathesaid:
I dnt knw if you jst bellyaching like fuck GT its got bad mojo now or what, and i personally don't like GT either, but that aint the cause of this shit.
Them contaminates can be very difficult to distinguish from cube myc and in many instances it goes undetected until it's melded seamlessly with cube myc.
Myco learning curve is a sharp bitch. Takes on average a year of consistent trial & error type work to round it. Dnt he hard on yourself or the variety, jst keep going.

Lol yeah i thnk that gag thing that people do is damn funny.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26497417 - 02/21/20 05:55 PM (3 years, 11 months ago)

Thank you for the encouraging words.
I'll keep at it. I have a very specific goal.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26497461 - 02/21/20 06:27 PM (3 years, 11 months ago)

Specific goal? You mind sharing?


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26497471 - 02/21/20 06:31 PM (3 years, 11 months ago)

Oh sure, I've been meditating for twenty years and before that a seeker from forever.
My goal is in laymans terms ego death, I have a barrier. It keeps me from... and I feel shrooms can break down that barrier.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26497483 - 02/21/20 06:37 PM (3 years, 11 months ago)

Yes they will. I would go for PE or pans


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Crack's Tek's and Shit



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OnlineLogicaL ChaosM
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26497484 - 02/21/20 06:38 PM (3 years, 11 months ago)

Anyone know where the "cleanest" spot of a spore print is?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26497514 - 02/21/20 06:59 PM (3 years, 11 months ago)

Quote:

Inthepit said:
Oh sure, I've been meditating for twenty years and before that a seeker from forever.
My goal is in laymans terms ego death, I have a barrier. It keeps me from... and I feel shrooms can break down that barrier.



Interesting. I practice a slower more additive kinda sober ego death. It's difficult but the practice burns karma.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26497984 - 02/22/20 05:33 AM (3 years, 11 months ago)

Well practices: Jhana, Vipassana, Zen, Liberation Unleashed, The Fetters, on and on...
Funny, in 1965 I put Buddhism as my religion in my Marine Corp file.


Edited by Inthepit (02/22/20 05:34 AM)


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: Inthepit]
    #26498124 - 02/22/20 08:20 AM (3 years, 11 months ago)

I just did my first blenderless Li yesterday.
Now I’m sitting staring at the jars like..

:awesketch:
DID IT WORK?!?!


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LAGM2020


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Offlinejdawg333
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26498137 - 02/22/20 08:28 AM (3 years, 11 months ago)

Can PF Tek be accomplished by steaming narrow mouthed quart sized jars of BRF and verm, placed into a larger container when ready?


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: jdawg333]
    #26498152 - 02/22/20 08:45 AM (3 years, 11 months ago)

Yes, you'll have to dig it out and you could make a shoebox


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26498156 - 02/22/20 08:50 AM (3 years, 11 months ago)

Quick question, should do transfers from a pin?
Or is a pin isolated enough already?
   
:shrug:  :hatsoff:


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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26498157 - 02/22/20 08:50 AM (3 years, 11 months ago)

Quote:

staytrippy420 said:
I just did my first blenderless Li yesterday.
Now I’m sitting staring at the jars like..

:awesketch:
DID IT WORK?!?!





Haha I can fully imagine. Plan on doing the same tomorrow ^^

Btw can I make blenderless LI from a half colonized agar plate? Just testing this out so not going to make loads of jars yet and my furthest plate atm is only about 50% colonized. If I just cut the myc part out and put it to the water jar should still work right?


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26498178 - 02/22/20 09:10 AM (3 years, 11 months ago)

Hell yeah man long as it’s clean and you fallow sterile procedure in the sab you should be good.. the hardest part for me was breaking the seal on my jar of sterilized water. Was a bitch to get off in the sab.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26498258 - 02/22/20 10:11 AM (3 years, 11 months ago)

:whathesaid: I've been having that issue of lids being a mofo, I've started flipping mine, seal up. Works good but lid slids easily. I have been wanting to look for tek on getting that seal from the bottom of lids off.


--------------------

Crack's Tek's and Shit



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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: Inthepit]
    #26498266 - 02/22/20 10:16 AM (3 years, 11 months ago)

Quote:

Inthepit said:
Quick question, should do transfers from a pin?
Or is a pin isolated enough already?
   
:shrug:  :hatsoff:





Did the pin come from a clean plate or was it from the germ plate?

I generally do At least 1 xfer from TIssue Culture or Pin Clone plate and make a grain jar to test from that, and put the TC/PC plate in cold storage, if the transfer grows out clean and the clone is a winner I’ll slant from the TC/PC plate and make a bunch of work plates.

if it was a germ plate I might still do 2-3 transfers.

Imo just based on looks that one needs another 1-2 to be clean, or your temps are swinging pretty drastically.


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