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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: flannel]
    #26495892 - 02/20/20 07:10 PM (3 years, 11 months ago)

Well not every single one.

Although that would be awesome.


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: A.k.a]
    #26496033 - 02/20/20 08:34 PM (3 years, 11 months ago)

I’ve always wondered why they don’t ALL turn into mushrooms?
Seems like there’s always more knots then actually fruits.


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26496039 - 02/20/20 08:39 PM (3 years, 11 months ago)

That's what they do though.  Some are only called to be knots.


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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: Edmunter]
    #26496440 - 02/21/20 05:02 AM (3 years, 11 months ago)

Quote:

Edmunter said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.





Update:

Shook the jars 1 week ago now. Mixed up dates in my previous post, so now they are 3 weeks after inoculation. Shaken at 2 week mark. How do these look? Looks slow to me compared to what i've been seeing here.











Currently experimenting with more grain jars with different soak/cook times and also some no soak/straight to PC. Also here growth seems slow.

Myc originated from different clones of the same growkit. Agar looks clean.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: fahtster]
    #26496459 - 02/21/20 05:22 AM (3 years, 11 months ago)

Am I isolating contam? This GT looks so weird.
I'm out to T6 now and no change.
       
:shrug:


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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26496479 - 02/21/20 06:01 AM (3 years, 11 months ago)

Personally I would have let it grown out a bit more before shake but that’s just me you would have probably had a quicker recovery time. Also there’s not much growth after a week so that could insinuate that it’s battling something. What are your ambient temps like and what is it you’re growing there?

These are my pesa 3 days after shake



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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26496484 - 02/21/20 06:09 AM (3 years, 11 months ago)

Quote:

Edmunter said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.





Update:

Shook the
Quote:

Smoothcat said:
Personally I would have let it grown out a bit more before shake but that’s just me you would have probably had a quicker recovery time. Also there’s not much growth after a week so that could insinuate that it’s battling something. What are your ambient temps like and what is it you’re growing there?

These are my pesa 3 days after shake






Its cubes clones, incubating in dark mostly in boiler room 20-25C. Plates looked clean. Thought maybe the grains are on the dry side.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26496489 - 02/21/20 06:16 AM (3 years, 11 months ago)

Yeah three weeks the jar should be done. Although maybe like you said it’s dry grains, I had a dry batch recently and it definitely slows things down.

Inthepit- my gt plates for lagm looked a lot like that at first but definitely cleaned up by t4. Although I did put it on plates with a recipe I get good rhizo from.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: A.k.a]
    #26496524 - 02/21/20 07:07 AM (3 years, 11 months ago)

Dry grains will definitely get you. I've got 3 Master jars going with dry oats, the worst grain to be dry IMO. I've made jars since that have passed them and will be ready soon.
I've been getting pretty good rhizo off of 1% agar and 1% nutes here lately. Thinking about upping the agar to 1.5 - 2.0 for a harder plate.


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OfflineCamera93
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26496538 - 02/21/20 07:27 AM (3 years, 11 months ago)

how do you best utilize duel compartment petri dishes?


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Invisiblerickyswamps
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Re: Cultivation General Discussion [Re: Camera93]
    #26496543 - 02/21/20 07:31 AM (3 years, 11 months ago)

Quote:

Camera93 said:
how do you best utilize duel compartment petri dishes?



For first and second transfers from spores.  When you know that you'll be transferring again being putting on grains/LC.  Sometimes you pick bad stuff from the germ plate on accident, so its good to have many T1's to look at.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: A.k.a]
    #26496545 - 02/21/20 07:33 AM (3 years, 11 months ago)

Quote:

A.k.a said:
Inthepit- my gt plates for lagm looked a lot like that at first but definitely cleaned up by t4. Although I did put it on plates with a recipe I get good rhizo from.




Thankyou and that recipie would be...drum roll...

My Rx is: 4g agar, 7g potato, 50% Oat broth, food color, for 500mL
:hatsoff:
Oh, and I microwaved agar from the fridge and the potato looked crappy. Not expecting mush out of this batch. However I used c10's method of pouring a little agar and swirling the plate to cover. Kinda cool...:cool:


Edited by Inthepit (02/21/20 07:38 AM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26496592 - 02/21/20 08:16 AM (3 years, 11 months ago)

lol I use like 6glme and 7-8g agar to 450ml.

Then I pour plates on the thin side. I’ve been doing all kinds of recipes to see what happens and  that’s been my go to, although I did just have a super fluffy aa+ culture go nice flat rhizo on t4 with thicker plates mixed like 5g lme to 10 agar.


Aa+ and gt were by far the fluffiest for me starting from spores. Just a big thick mess almost up to the lid until t3.


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OfflineInthepit
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Re: Cultivation General Discussion [Re: A.k.a]
    #26496614 - 02/21/20 08:33 AM (3 years, 11 months ago)

Inthepit said:
A.k.a said:
Inthepit- my gt plates for lagm looked a lot like that at first but definitely cleaned up by t4. Although I did put it on plates with a recipe I get good rhizo from.

Thankyou and that recipie would be...drum roll...

My Rx is: 4g agar, 7g potato, 50% Oat broth, food color, for 500mL
:hatsoff:
Oh, and I microwaved agar from the fridge and the potato looked crappy. Not expecting mush out of this batch. However I used c10's method of pouring a little agar and swirling the plate to cover. Kinda cool...:cool:
A.k.a said:
lol I use like 6glme and 7-8g agar to 450ml.

Then I pour plates on the thin side. I’ve been doing all kinds of recipes to see what happens and  that’s been my go to, although I did just have a super fluffy aa+ culture go nice flat rhizo on t4 with thicker plates mixed like 5g lme to 10 agar.


Aa+ and gt were by far the fluffiest for me starting from spores. Just a big thick mess almost up to the lid until t3.

I gotta get some lme, can't find a single brewing supply company on this pathetic island.
Gonna go begging at a brewery next.


Edited by Inthepit (02/21/20 09:13 AM)


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Invisiblerickyswamps
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Re: Cultivation General Discussion [Re: Inthepit]
    #26496617 - 02/21/20 08:36 AM (3 years, 11 months ago)

Who can recommend a stir plate for LC's?  And the type of stir bars that work the best.


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26496748 - 02/21/20 09:59 AM (3 years, 11 months ago)

Quote:

Beezorp said:
First time grow, spawn run started 10 days ago and fruiting conditions for the last 2-3. Using 100% coir with 1:1.25 ratio uncle ben spawn to coir. I think I see some hyphal knots but I'm still a noob. Also what are those larger clumps of mycelium with a slight yellow tinge? You can see what I mean near the top middle, slightly to the right. Thanks for any advice/observations!



Quote:

Inthepit said:
Am I isolating contam? This GT looks so weird.
I'm out to T6 now and no change.
       
:shrug:



First one is def not cube Myc. Second one just looks like MS culture but also could be something else.


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InvisibleLadysKnight
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Re: Cultivation General Discussion [Re: jbgtaa] * 1
    #26496760 - 02/21/20 10:06 AM (3 years, 11 months ago)

Agreed, first is mold.


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OfflineCamera93
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Re: Cultivation General Discussion [Re: Camera93]
    #26496842 - 02/21/20 11:08 AM (3 years, 11 months ago)

Quote:

Camera93 said:


So I bought that little induction heater linked in Pic of the day couple days ago
I got a 12V 2A power supply wired and it heats the knife up great..but only the 1st inch or so. If i pass the whole length of the tool in the coil it (power indicator) flashes which I assume means shorting out.

Can this be resolved with a higher amp supply?

I dont know much about electricity





Bought this power supply should be in tmr.

I haven't soldered the coil the board, hoping the short usage will keep it from melting the connectors

$40 (+ $12 I wasted on the 1st power supply), for an induction sterilizer

excited to get it all buttoned up and hope its functional


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinetedoro
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Re: Cultivation General Discussion [Re: A.k.a]
    #26496903 - 02/21/20 12:00 PM (3 years, 11 months ago)

Quote:

A.k.a said:


Aa+ and gt were by far the fluffiest for me starting from spores. Just a big thick mess almost up to the lid until t3.





This is so nice to hear. My GT is crazy fluffy at first.


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Edited by tedoro (02/21/20 12:02 PM)


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OfflineMushkingMulah360
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Re: Cultivation General Discussion [Re: Inthepit] * 1
    #26496912 - 02/21/20 12:05 PM (3 years, 11 months ago)

Personally I would trash them. Any plates I ever had turn out that way was early on and I didn’t even bother with them because I had many better plates. So it’s hard for me to 100 percent say it’s not cube myc but it don’t look right to say the least! Just my 2 cents. Shit if ya got the time and energy  grow it out throw it to grain and see what happens for experiment purposes. I’m sure it could help people down the line to see where it goes. If it makes you feel any better they were both plates from GT that did this, so maybe something to do with GT genetics


Edited by MushkingMulah360 (02/21/20 12:07 PM)


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