|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: Cultivation General Discussion [Re: MudaFuka]
#23926150 - 12/14/16 01:54 PM (7 years, 1 month ago) |
|
|
Which means that starting from spores solves any problem of senescence
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Cultivation General Discussion [Re: AlCapone2k]
#23926159 - 12/14/16 01:55 PM (7 years, 1 month ago) |
|
|
Unless you have a killer culture that you worked on for months and don't want to lose.
|
Greg
always learning



Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
Re: Cultivation General Discussion [Re: Tiamo]
#23926163 - 12/14/16 01:58 PM (7 years, 1 month ago) |
|
|
Quote:
Tiamo said: So what exactly is senescence and how do you counter it? Say you clone it, grow out the culture and take spores. If you grow out those spores, have you effectively "reset" the culture?
After a certain number of cell divisions the culture will slow down and in some cases lose the ability to fruit. If you store your cultures properly this shouldn't be too much of a concern. At least it isn't going to be a concern for quite a long time anyway.
Once you start from spores this process is reset.
Edited by Greg (12/14/16 02:03 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Cultivation General Discussion [Re: Greg]
#23926177 - 12/14/16 02:04 PM (7 years, 1 month ago) |
|
|
how many runs would that take? id imagine a shit load.
|
Greg
always learning



Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
Re: Cultivation General Discussion [Re: mushboy]
#23926191 - 12/14/16 02:07 PM (7 years, 1 month ago) |
|
|
Honestly I have no idea, probably a lot.
In addition to senescence from expanding a culture, I suspect that if you start from spores of a single line of genetics enough times, you will get an increase in zygosity and mutations and therefor a degradation of that line over time.
Edited by Greg (12/14/16 02:39 PM)
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Cultivation General Discussion [Re: mushboy]
#23926193 - 12/14/16 02:08 PM (7 years, 1 month ago) |
|
|
It depends on how you use your culture. If you just do a bunch of g2gs it can start slowing down after 3 or 4 runs. If you make transfers on agar and use those to inoculate grain it will last much longer. If you keep going back to your oldest plates and change you media it will last longer still.
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: Cultivation General Discussion [Re: azur] 1
#23926196 - 12/14/16 02:08 PM (7 years, 1 month ago) |
|
|
Quote:
azur said: I just use an assload of strainers. I can't stand messes and that looks like a mess
I use a bunch of 12 mesh bucket screens and a few buckets. Makes collecting a fuckton of grain water just about as easy as pouring into a bucket. No mess whatsoever.
If you don't know, a bucket screen is the top 1/4-1/3 of a bucket where the bottom 2/3 have been cut off and replaced with a screen melted into the plastic where the bottom portion was cut off from. A bucket screen can then just nest on top of an unmodified bucket to collect what makes it through the screen.
They are great for rinsing items like grains(if your grain prep calls for it) or even rocks for your cacti. If u use bucket screens to rinse a bag of rocks for cacti you can easily separate out the small grit and silt into the collection bucket below and pour off the water and let the sandy mix dry out and save that for cacti seedlings. etc. These things have tons of uses. Sifting powders to certain sizes for various applications. Some folks use them in the process of making honey I think too.
They are available in multiple screen sizes. 12, 20, 30, and 60 mesh screen sizes are available. The higher mesh means smaller openings between the screen wires. I actually have all sizes even 100 mesh, but those are all used for other projects.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Cultivation General Discussion [Re: Greg]
#23926211 - 12/14/16 02:13 PM (7 years, 1 month ago) |
|
|
Quote:
MudaFuka said: It depends on how you use your culture. If you just do a bunch of g2gs it can start slowing down after 3 or 4 runs. If you make transfers on agar and use those to inoculate grain it will last much longer. If you keep going back to your oldest plates and change you media it will last longer still.
Quote:
Greg said: Honestly I have no idea, probably a lot.
I addition to senescence from expanding a culture, I suspect that if you start from spores of a single line of genetics enough times, you will get an increase in zygosity and mutations and therefor a degradation of that line over time.
good shit. thank you. answered another question i was pondering as well.(why change media)
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 7 hours, 16 minutes
|
Re: Cultivation General Discussion [Re: mushboy]
#23926295 - 12/14/16 02:36 PM (7 years, 1 month ago) |
|
|
Thanks for the answers guys!
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Cultivation General Discussion [Re: Juiceh]
#23926311 - 12/14/16 02:43 PM (7 years, 1 month ago) |
|
|
Quote:
Juiceh said:
Quote:
azur said: I just use an assload of strainers. I can't stand messes and that looks like a mess
I use a bunch of 12 mesh bucket screens and a few buckets. Makes collecting a fuckton of grain water just about as easy as pouring into a bucket. No mess whatsoever.
If you don't know, a bucket screen is the top 1/4-1/3 of a bucket where the bottom 2/3 have been cut off and replaced with a screen melted into the plastic where the bottom portion was cut off from. A bucket screen can then just nest on top of an unmodified bucket to collect what makes it through the screen.
They are great for rinsing items like grains(if your grain prep calls for it) or even rocks for your cacti. If u use bucket screens to rinse a bag of rocks for cacti you can easily separate out the small grit and silt into the collection bucket below and pour off the water and let the sandy mix dry out and save that for cacti seedlings. etc. These things have tons of uses. Sifting powders to certain sizes for various applications. Some folks use them in the process of making honey I think too.
They are available in multiple screen sizes. 12, 20, 30, and 60 mesh screen sizes are available. The higher mesh means smaller openings between the screen wires. I actually have all sizes even 100 mesh, but those are all used for other projects.
Do you make those buckets or you get them from a store? Any pics?
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: Tiamo]
#23926408 - 12/14/16 03:02 PM (7 years, 1 month ago) |
|
|
Quote:
Tiamo said: So what exactly is senescence and how do you counter it? Say you clone it, grow out the culture and take spores. If you grow out those spores, have you effectively "reset" the culture?
It is basically too much cell division
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
|
Re: Cultivation General Discussion [Re: cronicr]
#23926593 - 12/14/16 03:42 PM (7 years, 1 month ago) |
|
|
For those of you that run hoods, do you like cooling spawn and sub bags in front of the hood or in a tote? I have done both, I have just been concerned with the longevity of my filter lately not wanting to run it if I don't have to, so I'm just curious how many of you guys still cool your spawn in totes even though you run a hood, also I use the tyvek sleeves for sterilizing if that makes a difference to anyone.
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: Cultivation General Discussion [Re: Marty Mycfly]
#23926599 - 12/14/16 03:44 PM (7 years, 1 month ago) |
|
|
Air filter will last for a long long time it taken care of. You can run it 24 hours a day if you want. I prefer to let mine cool down in the pressure cooker before I take them out
|
Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
|
Re: Cultivation General Discussion [Re: azur]
#23926621 - 12/14/16 03:50 PM (7 years, 1 month ago) |
|
|
Thanks Azur, I let them cool in there if I sterilize at night to use the next day, but I have a batch just about to be done right now and was hoping to get them inoculated before bed tonight, I planned on letting them cool for at least a few hours in there before pulling them out. I will just go ahead and cool them in front of the hood, they cool way quicker that way anyways.
|
ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
|
Re: Cultivation General Discussion [Re: Mad Season]
#23926662 - 12/14/16 04:03 PM (7 years, 1 month ago) |
|
|
I haven't run into grain moisture issues yet. Knock on wood. After I boil them I just strain and immediately load jars. You can see some extra moisture where the grains touch the glass, but that goes away soon after inoculation. Maybe only a day after?
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Cultivation General Discussion [Re: Tiamo] 1
#23926788 - 12/14/16 04:38 PM (7 years, 1 month ago) |
|
|
Quote:
Tiamo said: So what exactly is senescence and how do you counter it? Say you clone it, grow out the culture and take spores. If you grow out those spores, have you effectively "reset" the culture?
Everytime a cell divides the telomeres on the ends of the chromosomes get shorter. As they get too short errors start to occur. This leads to the cells being formed incorrectly. Examples of senescence in humans is wrinkles, grey hair, hair loss. It's basically a fancy word for aging.
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
Re: Cultivation General Discussion [Re: Pastywhyte] 1
#23926910 - 12/14/16 05:09 PM (7 years, 1 month ago) |
|
|
Awesome example pasty.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Cultivation General Discussion [Re: van hatton]
#23927115 - 12/14/16 05:57 PM (7 years, 1 month ago) |
|
|
Yeah
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Cultivation General Discussion [Re: Kenetic]
#23928836 - 12/15/16 10:24 AM (7 years, 1 month ago) |
|
|
i did not want to make a thread for this because i feel like im going to when i get the answer
could someone clear up what the best traits are to look for when taking transfers. ive read stros and most others but once i get a grasp of what i am looking for, i read further and realize i am really still confused and have no clue what im doing. Im not taking about cleaning. but best traits ...besides 'look for really rhizo growth'. im looking for further explanation i guess.
currently i have my first cloned tissue grown on a plate. it pinned and i took that pin another plate and its growing nicely. what am i looking for in the next transfer? or should i just put it to grain. its super clean looking. id take a pic but glad rounds are quickly showing a drawback. i cant see my fucking cultures in detail until i open them in the sab. thanks.
|
The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 9 hours
|
Re: Cultivation General Discussion [Re: mushboy]
#23928861 - 12/15/16 10:35 AM (7 years, 1 month ago) |
|
|
I dont think it really matters. If its clean and not super slow growing then you are good. Good cultures can be rhizo sometimes and tomentose other times. I think a good tek is to just get a clean culture, use that culture for a monotub and then clone a fruit that shows ideal characteristics.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

|
|