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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 7 hours, 9 minutes
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Re: Cultivation General Discussion [Re: filthyknees]
#26489550 - 02/17/20 07:21 AM (3 years, 11 months ago) |
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Quote:
c10h12n2o said:
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Camera93 said:

So I bought that little induction heater linked in Pic of the day couple days ago I got a 12V 2A power supply wired and it heats the knife up great..but only the 1st inch or so. If i pass the whole length of the tool in the coil it (power indicator) flashes which I assume means shorting out.
Can this be resolved with a higher amp supply?
I dont know much about electricity
That's really cool, I guess it's a DIY bacticinerator. How long does it take to get red hot? My bacticinerator takes about 5 or 6 seconds

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GypsyCurse said: Well, it says max power is 120 watts which wud be drawing 10amps at 12volts. Your power supply says 2amps. Need more power. Anything allowing a 10amp pull is gonna have thicker gauge wire than that little power supply.
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tramalot said: Amazon shows a 5 amp supply normally sold with it!
I'd go 7.5-10amp
Takes about 5 seconds or so. That's the idea, just as much cheaper alternative.
Someone mentioned writing up something on a DIY but I haven't seen it. I seen the induction heater linked tho and it was pretty cheap, that plus a power supply under $40 for a sterilizer
I'm gonna get a higher rated supply so I can have it do what I want. I'll also likely solder the coil to the device
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
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Re: Cultivation General Discussion [Re: Camera93]
#26489588 - 02/17/20 07:57 AM (3 years, 11 months ago) |
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I had posted about this in the Agar Envy thread, but i'm also having bacterial issues with oats. I want to take responsibility and blame my cultures not being 100% clean, but man it does make me wonder if oats are just bad. I have another 50# to work through so i'm just going to keep trudging forward, cleaning my cultures as carefully as i can.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26489599 - 02/17/20 08:15 AM (3 years, 11 months ago) |
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No matter how clean I get my cultures I keep having issues and I'm starting to think it may be my oats. I'm switching back to WBS.
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: Cultivation General Discussion [Re: Crackatoa]
#26489623 - 02/17/20 08:37 AM (3 years, 11 months ago) |
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wheat is the best of both worlds. Price of Oats, quality of Rye.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26489627 - 02/17/20 08:39 AM (3 years, 11 months ago) |
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Quote:
p9hu7 said: Some oat specific bacteria that survives sterilization?
Or it could be people being noobs with grain prep. That's what I blame my oat failures on because I've certainly done numerous grows with oats that kicked ass.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Nichrome]
#26489631 - 02/17/20 08:41 AM (3 years, 11 months ago) |
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Quote:
Nichrome said: It's all batch dependant. Rye seems more consistent. Hard rye seed is the bestest. Feed grains are usually crap. Seed grains need to retain 99% viability. Seed grains are the bestest.
yes, the seed grains are worth their price IMO
@ricky: I would say wheat is not quite on par with quality as rye but that can be overlooked for the cost
@mushboy: I have a bag I can send u some oats if u want. I've prepped them every way and sterilized the crap out of em. even done multiple cycles. nothing I do can get them to colonize before going to crap.
go buy some grains from the bulk bin at your grocery and if you still cant get clean growth on em then maybe its the grain. when I had issues it wasnt just oats but every grain from a certain supplier. simply switching brands did the trick
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: TedsDead]
#26489632 - 02/17/20 08:41 AM (3 years, 11 months ago) |
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How do you know my noob ass wont botch the prep?
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: mushboy]
#26489636 - 02/17/20 08:43 AM (3 years, 11 months ago) |
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I'll have to do that for you I guess you gotta cover the cost difference to ship tho
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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watsuu
Stranger

Registered: 01/08/20
Posts: 25
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Re: Cultivation General Discussion [Re: TedsDead]
#26489643 - 02/17/20 08:51 AM (3 years, 11 months ago) |
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Oh god i have my first ever pins
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: mushboy]
#26489982 - 02/17/20 01:04 PM (3 years, 11 months ago) |
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Quote:
mushboy said:
Quote:
p9hu7 said: Some oat specific bacteria that survives sterilization?
Or it could be people being noobs with grain prep. That's what I blame my oat failures on because I've certainly done numerous grows with oats that kicked ass.
My attempt at WBS was pretty noobtastic, Worst grain prep failure I've ever had.
I'm getting a bag of wheat and giving that a whirl. Oats have become insufferable, I've grown to hate grain prep because of them.
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natedawgnow said: It's been shown in lots of studies that hull-intact cereal grains have higher bacterial/endo spore counts.
I was unaware of that, thanks.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26489993 - 02/17/20 01:11 PM (3 years, 11 months ago) |
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Wheat is awesome! Best grain I've worked with and at 12 bucks for 50lbs the price is pretty comparable to oats.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: natedawgnow]
#26490075 - 02/17/20 01:57 PM (3 years, 11 months ago) |
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Jst have some wbs and some wheat/rye berries on hand and you'll be fine. You dnt need any of that other shit that is generally inconsistent in quality. Dnt buy organic either. They dump a bunch of shit on it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: natedawgnow]
#26490076 - 02/17/20 01:58 PM (3 years, 11 months ago) |
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All I know is none of my unopened sterilized jars of oats went bad on their own.
They only went to shit after I opened them. Leading me to point the finger at myself.
Got links to those studies Nate? None of the articles I saw were taking about sterilized grain. It was all cereal grains affecting the guts bio blahblah.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: mushboy]
#26490117 - 02/17/20 02:22 PM (3 years, 11 months ago) |
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Obviously the studies aren't about sterilized grains, dude. It's about the bacteria that's endemic to the grains and how hull-intact grains have more spores as compared to hulled grains because, inherently, the majority of contaminant spores are found on the surface of grains. If the hull is still intact, higher amounts of spores can be found between the hull and the grain.
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Edited by natedawgnow (02/17/20 02:23 PM)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: natedawgnow]
#26490142 - 02/17/20 02:33 PM (3 years, 11 months ago) |
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You can read the whole article but here is some relevant info https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.14181
Quote:
The typical structure of a cereal grain constitutes of three edible parts: the bran which consists of the outer coat (pericarp, testa, and aleurone layers), the germ (the embryo) and the starchy endosperm, and an inedible husk that protects the kernel (Figure 4) (Dexter & Wood, 1996; Merali et al., 2013). Microbial colonization is generally restricted to the outer layers of cereal grains, that is, the husk, between the husk and pericarp, and within the pericarp tissue (Briggs, 1998). Several studies showed that after debranning—a controlled process in which the outer layers of the grains are removed, cereals are microbiologically purer (Bainotti & Perez, 2000; Laca et al., 2006). However, there are species able to invade the inner part of the grains and penetrate into the endosperm, causing internal infections (Nierop, 2006).
This ones just an abstract about wheat but go ahead and check it out anyway https://www.researchgate.net/publication/223382603_Distribution_of_microbial_contamination_within_cereal_grains
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: natedawgnow]
#26490320 - 02/17/20 03:40 PM (3 years, 11 months ago) |
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I guess I'm just confused because we sterilize the grain.
So isnt the bacterial/endo load in oats(or intact hull grain) a moot point?
Thx faht
Edited by mushboy (02/17/20 03:42 PM)
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spiritlands



Registered: 06/21/10
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26490385 - 02/17/20 04:17 PM (3 years, 11 months ago) |
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Quote:
GypsyCurse said: So check this out... I got a 1/2lb Ape/Pe mix thats powdered and stored in two pint mason jars awaiting extraction. How long will it keep?
Hey so keep me posted on that. I made some moonshine and I've got an AA+ mono fruiting right now. Had some tubs go bad lately bc I was using up leftover spawn that weren't my first choice jars. Hopefully be running that extraction we talked about next week. I wanna run the extraction/conversion on some pans but I gotta stop eating them first.
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: mushboy]
#26490397 - 02/17/20 04:21 PM (3 years, 11 months ago) |
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What we do is more like 99.9%(effective sterilization) as opposed to 100%(complete sterilization, whether there are 100,000,000,000 or 1,000,000 or 1,000 actually does make a difference because there will be some level of survival, and its also possible the hulls act as insulation making sterilization even less effective
(All numbers fabricated to make a point)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: AyePlus]
#26490406 - 02/17/20 04:25 PM (3 years, 11 months ago) |
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Even knowing that I'll still blame my oat issues on my hand not the grain.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: mushboy]
#26490473 - 02/17/20 05:07 PM (3 years, 11 months ago) |
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Quote:
mushboy said: I guess I'm just confused because we sterilize the grain.
So isnt the bacterial/endo load in oats(or intact hull grain) a moot point?
Thx faht
The point is you start out with a grain with a higher endospore count. An endospore is a small spore inside of bacteria cells which can die off but still leave a living endospore. If we sterilize up to 99.99%, then out of 10,000,000 cells 100,000 of them still survive. Plus the hull could aid in protection.
Just something to think about
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Edited by natedawgnow (02/17/20 05:09 PM)
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