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Invisibletramalot
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Re: Cultivation General Discussion [Re: tedoro]
    #26484720 - 02/14/20 05:42 AM (3 years, 11 months ago)

Quote:

tedoro said:
Quote:

gizmo1 said:
Quote:

tedoro said:
I've tried everything. I'm currently using unmodified 28q under bed sterilites, which are quite breezy. I add gypsum to my grains, stopped adding to my coir. I'll start again. How much do you add to, say a brick?



I always go for a handful I dont measure at all. Im pretty sure gypsum doesn't do much for PH though. It will change it a little but its not going to fix your mold problem. What finally helped me beat my 4 year battle with mold was delving deeper into agar and really learning to examine and get an eye for it. Tightening up my sterile tek, and last but certainly not least I've been posting everything to shroomery and having the good folks here help me to judge my plates and spawn while at the same time training my eye to recognize the good from the bad and the ugly. Anything sort of questionable I straight toss into the compost. I get plenty of garden mushrooms though so its not a complete waste.




I'm all in on questioning my agar work. I was running my mycelia too long, so I started fresh from spores. My plates are needing a few more transfers to look good. I got impatient and put some to grain... they are currently growing out. What I found frustrating about this stage of my knowledge is that 1) my plates always looked fine 2)spawn grew out fine and smelled fine 3)tubs grew fine, until full consolidation.... then green. I seem to only struggle with invisible or extremely hard to perceive problems. If I would have known that from the beginning, I would have played with agar for a few months and posted my plates for critique. I believe thats where my success lies. The cleanliness in my grow area is a side interest. (and I find it controversial and that interests me)




I'm finding if I let it consolidate 5 extra day's contamination drops off.


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Invisiblefilthyknees
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Re: Cultivation General Discussion [Re: tramalot]
    #26484724 - 02/14/20 05:46 AM (3 years, 11 months ago)

For condensation, I pour 40 plates or so at a time, once cooled I'll stack all the ones with condensation (top 3-4) in their own stack to be re-sleeved, the other 30 or so are ready to use immedietly, and by the time I'm done with them the condensated ones would have gone clear.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26484730 - 02/14/20 05:50 AM (3 years, 11 months ago)

Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




Welcome back :awesomenod:

I don’t use rye I use millet and I don’t soak I just cook with the right amount of water to hydrate my grain, however those grains do look dry and if I remember rightly the soak is 12-24 hours, also 3 weeks is a long time, I’d suggest doing some more grain prep as those probably ain’t gonna finish imo

I also went away for a while and I needed to do a lot of refresher reading before I got going again. Good luck :thumbup:


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Offlinetedoro
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Re: Cultivation General Discussion [Re: tramalot]
    #26484740 - 02/14/20 05:58 AM (3 years, 11 months ago)

Quote:

tramalot said:
Quote:

tedoro said:
Quote:

gizmo1 said:
Quote:

tedoro said:
I've tried everything. I'm currently using unmodified 28q under bed sterilites, which are quite breezy. I add gypsum to my grains, stopped adding to my coir. I'll start again. How much do you add to, say a brick?



I always go for a handful I dont measure at all. Im pretty sure gypsum doesn't do much for PH though. It will change it a little but its not going to fix your mold problem. What finally helped me beat my 4 year battle with mold was delving deeper into agar and really learning to examine and get an eye for it. Tightening up my sterile tek, and last but certainly not least I've been posting everything to shroomery and having the good folks here help me to judge my plates and spawn while at the same time training my eye to recognize the good from the bad and the ugly. Anything sort of questionable I straight toss into the compost. I get plenty of garden mushrooms though so its not a complete waste.




I'm all in on questioning my agar work. I was running my mycelia too long, so I started fresh from spores. My plates are needing a few more transfers to look good. I got impatient and put some to grain... they are currently growing out. What I found frustrating about this stage of my knowledge is that 1) my plates always looked fine 2)spawn grew out fine and smelled fine 3)tubs grew fine, until full consolidation.... then green. I seem to only struggle with invisible or extremely hard to perceive problems. If I would have known that from the beginning, I would have played with agar for a few months and posted my plates for critique. I believe thats where my success lies. The cleanliness in my grow area is a side interest. (and I find it controversial and that interests me)




I'm finding if I let it consolidate 5 extra day's contamination drops off.




OOOOoooo thats interesting. I've been sloppy in the past about how many days of consolidation I do, then I recently read that over 2 days can be problematic.... making the mycelia change gears. I still don't document my consolidation well... giving me little feedback from what helps. I will from here on out. (and I'll try 5 days)


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/14/20 05:59 AM)


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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26484742 - 02/14/20 05:59 AM (3 years, 11 months ago)

Quote:

Smoothcat said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




Welcome back :awesomenod:

I don’t use rye I use millet and I don’t soak I just cook with the right amount of water to hydrate my grain, however those grains do look dry and if I remember rightly the soak is 12-24 hours, also 3 weeks is a long time, I’d suggest doing some more grain prep as those probably ain’t gonna finish imo

I also went away for a while and I needed to do a lot of refresher reading before I got going again. Good luck :thumbup:




Thanks!

Yes Im definetely making some more jars. Thinking of letting it soak longer and a shorter time to evaporate after cooking. For this batch I let it sit in coriander and shook the grains up after cooking for 10 mins until all the steam was gone (30-45 mins) and they felt dry to the touch. Maybe overdone this step a bit as I thought better not make it too wet... ^^


Edited by Infinity-25 (02/14/20 06:01 AM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26484771 - 02/14/20 06:19 AM (3 years, 11 months ago)

Quote:

Infinity-25 said:
Quote:

Smoothcat said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




Welcome back :awesomenod:

I don’t use rye I use millet and I don’t soak I just cook with the right amount of water to hydrate my grain, however those grains do look dry and if I remember rightly the soak is 12-24 hours, also 3 weeks is a long time, I’d suggest doing some more grain prep as those probably ain’t gonna finish imo

I also went away for a while and I needed to do a lot of refresher reading before I got going again. Good luck :thumbup:




Thanks!

Yes Im definetely making some more jars. Thinking of letting it soak longer and a shorter time to evaporate after cooking. For this batch I let it sit in coriander and shook the grains up after cooking for 10 mins until all the steam was gone (30-45 mins) and they felt dry to the touch. Maybe overdone this step a bit as I thought better not make it too wet... ^^




I'd cook them a bit longer too.... get more water in there. a few more minutes can add a lot of water to the inside of the grains.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineCamera93
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Re: Cultivation General Discussion [Re: tedoro]
    #26484823 - 02/14/20 06:57 AM (3 years, 11 months ago)

I have 2 LC syringes from a vendor. Lions mane and Reishi, I haven't used either of them yet and I made up 7-8 150ml LC broths last night.
My question is, how risky is it to noc up a couple of my broths from the vendor syringe?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: Camera93]
    #26484851 - 02/14/20 07:18 AM (3 years, 11 months ago)

I’d be interested in that too as I literally received my reishi lc this morning and was wandering my best way forward


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26484859 - 02/14/20 07:25 AM (3 years, 11 months ago)

Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26484864 - 02/14/20 07:28 AM (3 years, 11 months ago)

Quote:

Smoothcat said:
I’d be interested in that too as I literally received my reishi lc this morning and was wandering my best way forward




I grew them in a bag invitro and they worked really well.



The hole was too big but next one will be the nuts......


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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: Edmunter]
    #26484921 - 02/14/20 08:07 AM (3 years, 11 months ago)

Quote:

Edmunter said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.




Thanks for your input. I just gave them a good shaking. Lets see what happens..


In addition, I knocked up some smaller jars 1 week later (from the same agar plate I used for the first ones, not so smart probably but myc looked good still on agar), so 2 weeks ago today. Soaked these for 10 hours prior to cooking:






Both pics are from the same plate. The 2nd one looks contaminated to me as is has a distinct fuzzier patch next to the myc. What do you guys think? First one looks healthy with 2 weeks of growth?

I made an own thread of it earlier this week with some more info if someone is interested: https://www.shroomery.org/forums/showflat.php/Number/26478087


Edited by Infinity-25 (02/14/20 08:09 AM)


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26484952 - 02/14/20 08:31 AM (3 years, 11 months ago)

Quote:

Infinity-25 said:
Quote:

Edmunter said:
Quote:

Infinity-25 said:
Cool thread!

Im just restarting growing again. Knocked up some rye jars with clone myc I grew on agar. Getting slow growth, in particular in middle jar. Time is now 3 weeks after inocculation. no shake yet.



Thinking this could be due to grains too dry (RR method, soaked 6hrs) or possible contam in the middle jar (different clone than other 2 jars). Curious about opinions of better trained eyes :smile:




I dont think that -not perfect on the dry side grains slows culture down that much and IMHO I think those grains look ok.  What might not be ok is that they have a contamination of some kind.  Im getting full colonisation on any grains 7-21 days max.  If its any longer and its standard cubes im worrying.  Shake them and see what happens.  The fussy bits around the edges look like they might go moldy.  Im interested to see the outcome, please post.




Thanks for your input. I just gave them a good shaking. Lets see what happens..


In addition, I knocked up some smaller jars 1 week later (from the same agar plate I used for the first ones, not so smart probably but myc looked good still on agar), so 2 weeks ago today. Soaked these for 10 hours prior to cooking:






Both pics are from the same plate. The 2nd one looks contaminated to me as is has a distinct fuzzier patch next to the myc. What do you guys think? First one looks healthy with 2 weeks of growth?

I made an own thread of it earlier this week with some more info if someone is interested: https://www.shroomery.org/forums/showflat.php/Number/26478087




Agreed but im a bit wary of those grains...... lets see


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OfflineCamera93
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Re: Cultivation General Discussion [Re: Edmunter]
    #26484963 - 02/14/20 08:40 AM (3 years, 11 months ago)

@Edmunter

Nice Reishi!

But what were your thoughts on expanding the culture via:
Vendor LC syringe  ->  LC broth ?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineStrohngBahd
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Re: Cultivation General Discussion [Re: Camera93] * 1
    #26484970 - 02/14/20 08:45 AM (3 years, 11 months ago)

Test the syringe on agar first, if its clean you're good to expand it


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OfflineInfinity-25
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Re: Cultivation General Discussion [Re: tedoro]
    #26485069 - 02/14/20 09:44 AM (3 years, 11 months ago)

Quote:



I'd cook them a bit longer too.... get more water in there. a few more minutes can add a lot of water to the inside of the grains.




Hmm I understood from RR'r notes that 10min was optimal cooking time. I had a few burst kernels, not many though. Ill experiment with 2-3 minutes more :thumbup:


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Offlinefootpath
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Re: Cultivation General Discussion [Re: Infinity-25]
    #26485098 - 02/14/20 09:58 AM (3 years, 11 months ago)

I'm pretty sure he said that it's basically a sliding scale - if you soak for 6 hours, you need to boil longer. If you soak for 24 hours, you need to boil shorter.

Personally, I've been just cooking mine at just below boiling temperatures for about 40 minutes. No soak. A little more burst grains, but nothing I'd consider excessive or problematic.


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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: footpath]
    #26485104 - 02/14/20 10:02 AM (3 years, 11 months ago)

I don’t soak or boil, just pc everything together to get the right moisture content


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Invisibletramalot
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26485112 - 02/14/20 10:09 AM (3 years, 11 months ago)

Would that process of doing everything at once have a "Tek" name?

Thanks


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26485116 - 02/14/20 10:10 AM (3 years, 11 months ago)

That’s how I use to roll.. it’s so fast.

Quote:

tramalot said:
Would that process of doing everything at once have a "Tek" name?

Thanks




I’ve got one in my sig

Faht


Edited by fahtster (02/14/20 10:12 AM)


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Invisibletramalot
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Re: Cultivation General Discussion [Re: fahtster]
    #26485125 - 02/14/20 10:17 AM (3 years, 11 months ago)

I haven't looked yet but thanks for the quick reply!


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