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OfflineSavoryGravy
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Re: Cultivation General Discussion [Re: feldman114]
    #26466746 - 02/03/20 12:25 PM (3 years, 11 months ago)

Quote:

A.k.a said:
How much impact cam leaving a moldy tub in your room for a few days actually have??

I’ve had 5 of my last 6 shoeboxes get some kind of green mold either right at the start of or right after the second flush. I don’t think it’s trich it’s not very aggressive but similar look.

So of the five two were ms to grain and two were questionable plates, so that’s not surprising, but the last one was a clean plate and as far as I could tell clean spawn. Looked really healthy and came back after the shake immediately so that’s whats got me worried.

I’ve got like 30 qts that’ll be done over the next week or so which is a huge amount for me and also will be my first 5qt tubs so I don’t want to risk losing them to something as simple as wiping down the room.

When it comes up on here though it seems like people are pretty polarized and either don’t think it makes much difference unless you’re open fruiting trich or they’re super OCD about spores.

Either way I’ll be doing some level of cleaning but I’m curious about this now.





Pretty sure that's what's happening to me. Got mold in jars, shook them out over the trashcan (and all over the house), apparently didn't clean the jars enough, next 20 went bad.:frown:


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Offlinetedoro
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Re: Cultivation General Discussion [Re: SavoryGravy]
    #26466753 - 02/03/20 12:28 PM (3 years, 11 months ago)

I've been poorly growing cubes in my space for 10 years, meaning every tub trichs out, then I toss it. I clean, but not that much (leafblower the place, let it settle the mop the floor twice). I'm now thinking that coir might have a limit to its resistance, and people vary with what they consider clean.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: Cultivation General Discussion [Re: A.k.a]
    #26466757 - 02/03/20 12:30 PM (3 years, 11 months ago)

Quote:

A.k.a said:
Yeah for sure. I’m getting better at recognizing what’s going on with spawn now that I’m using agar.

Right now my main thing is how it comes back after shaking. I have two jars I was super careful and clean with, payed attention to how the growth off the wedges looked and how it did after a shake and now I compare everything to those. I definitely have a couple right now that are bacterial but the kind that makes the myc too thick, and a month ago I would’ve thought it was good to go.




Eats has been helping me with making bomb LI for my pre-sealed bags. But my plates haven't been cleaned all the way. I think I see spots of thicker growth like you say.

https://www.shroomery.org/forums/showflat.php/Number/26466701#26466701


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/03/20 12:30 PM)


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: tedoro]
    #26466763 - 02/03/20 12:34 PM (3 years, 11 months ago)

Quote:

tedoro said:
I've been poorly growing cubes in my space for 10 years, meaning every tub trichs out, then I toss it. I clean, but not that much (leafblower the place, let it settle the mop the floor twice). I'm now thinking that coir might have a limit to its resistance, and people vary with what they consider clean.



10 years with 0 success.
Please lend some of your calm to the “Getting Started” forum. People loose their mind if they don’t see pins 3 days after spawning.

But srsly, this is easy enough to figure out. Put a piece of tammed sub in a bag for 2-3 days, hydrate some coir in a bucket, open bag over bucket, wait.
I’m guessing the coir will stay clean for at least a week or 2 though. Longer than it would take to colonize anyway.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: feldman114]
    #26466772 - 02/03/20 12:41 PM (3 years, 11 months ago)

Quote:

feldman114 said:
Quote:

tedoro said:
I've been poorly growing cubes in my space for 10 years, meaning every tub trichs out, then I toss it. I clean, but not that much (leafblower the place, let it settle the mop the floor twice). I'm now thinking that coir might have a limit to its resistance, and people vary with what they consider clean.



10 years with 0 success.
Please lend some of your calm to the “Getting Started” forum. People loose their mind if they don’t see pins 3 days after spawning.

But srsly, this is easy enough to figure out. Put a piece of tammed sub in a bag for 2-3 days, hydrate some coir in a bucket, open bag over bucket, wait.
I’m guessing the coir will stay clean for at least a week or 2 though. Longer than it would take to colonize anyway.




Dude, I've been so unlucky, I just love this hobby. And I will prevail.

My only hesitation with that test is that these colonizing monos are sweaty and 2,3,4 degrees warmer than room temp... making a slightly more crappy environ than a bucket with spores on it sitting mellow at room temp. maybe put a 5 watt nightlight under the bucket.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/03/20 12:44 PM)


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Offlinewatsuu
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Registered: 01/08/20
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Re: Cultivation General Discussion [Re: tedoro]
    #26466829 - 02/03/20 01:35 PM (3 years, 11 months ago)



What do you guys think?
Made this shoebox 5 days ago (I think, i really should label things :shrug:) out of 2 uncle bens bags and eyeballed 1:1 ratio coir
At least no contams yet. Last two seemed fine for the first week too but then the green came along on the top, this time the myc seems to be taking over nicely so hopefully nothing bad grows on it?


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: watsuu]
    #26466835 - 02/03/20 01:42 PM (3 years, 11 months ago)

Quote:

watsuu said:


What do you guys think?
Made this shoebox 5 days ago (I think, i really should label things :shrug:) out of 2 uncle bens bags and eyeballed 1:1 ratio coir
At least no contams yet. Last two seemed fine for the first week too but then the green came along on the top, this time the myc seems to be taking over nicely so hopefully nothing bad grows on it?




Looks healthy and ready for FAE.
Next time, add a 1/2in top layer of coir. And even it out better maybe.


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Offlinewatsuu
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Re: Cultivation General Discussion [Re: feldman114]
    #26466843 - 02/03/20 01:47 PM (3 years, 11 months ago)

Quote:

feldman114 said:
Quote:

watsuu said:


What do you guys think?
Made this shoebox 5 days ago (I think, i really should label things :shrug:) out of 2 uncle bens bags and eyeballed 1:1 ratio coir
At least no contams yet. Last two seemed fine for the first week too but then the green came along on the top, this time the myc seems to be taking over nicely so hopefully nothing bad grows on it?




Looks healthy and ready for FAE.
Next time, add a 1/2in top layer of coir. And even it out better maybe.



Last two had a layer of coir on top and then the round white spots appeared and couple of days later thick white growth and later the green came.

Im using this kind of shoebox https://plast-team.com/product/basic-box-shoebox-small/ with one of the sides not clicked in completelly so theres a little of airgap, should i just rest the lid on top?


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: watsuu]
    #26466856 - 02/03/20 01:54 PM (3 years, 11 months ago)

Quote:

watsuu said:
Quote:

feldman114 said:
Quote:

watsuu said:


What do you guys think?
Made this shoebox 5 days ago (I think, i really should label things :shrug:) out of 2 uncle bens bags and eyeballed 1:1 ratio coir
At least no contams yet. Last two seemed fine for the first week too but then the green came along on the top, this time the myc seems to be taking over nicely so hopefully nothing bad grows on it?




Looks healthy and ready for FAE.
Next time, add a 1/2in top layer of coir. And even it out better maybe.



Last two had a layer of coir on top and then the round white spots appeared and couple of days later thick white growth and later the green came.

Im using this kind of shoebox https://plast-team.com/product/basic-box-shoebox-small/ with one of the sides not clicked in completelly so theres a little of airgap, should i just rest the lid on top?




When in doubt, stick to the tek.

The green didn’t come from coir - it’s resistant. It came from your rice, which I’m guessing you knocked up with a dirty MS syringe.

A top layer mostly just helps with the pinset, since cubes don’t like to pin on grains.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: feldman114]
    #26466860 - 02/03/20 01:56 PM (3 years, 11 months ago)

Yeah top layers are the shit.

Definitely gotta level that out more next time, or even add a top layer now and level it.


--------------------
LAGM2020


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Offlinewatsuu
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Registered: 01/08/20
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Re: Cultivation General Discussion [Re: A.k.a]
    #26466878 - 02/03/20 02:03 PM (3 years, 11 months ago)

Well, if my syringes are contaminated I hope i can atleast get something out of this shoebox.
I do have some mycelium currently growing on agar so at least I got that going on.

Also, im bit worried about messing with this thing too much so i think im going to just see where it goes.
This third box i have already is heaps better than the two i had, i mixed the spawn and coir much better and this is actually pretty level compared to the other two :shrug:


Edited by watsuu (02/03/20 02:11 PM)


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: watsuu]
    #26466896 - 02/03/20 02:12 PM (3 years, 11 months ago)

Quote:

watsuu said:
Well, if my syringes are contaminated I hope i can atleast get something out of this shoebox.
I do have some mycelium currently growing on agar so at least I got that going on.




Syringes are never clean, that’s why we “clean” them up on agar.
They’re good for cakes and such, but not for stuff you’re planning to spawn to bulk.

This is coming from an ex-agar denier lol...thought I had clean spawn for months, didn’t get why people don’t use MS->grain, but it only looked clean. Agar only looks complicated btw - it’s the easiest step of a grow.


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OfflineeLeSDenes
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Re: Cultivation General Discussion [Re: watsuu]
    #26466902 - 02/03/20 02:17 PM (3 years, 11 months ago)

It depends whether you have your inoculation area at the same room or not. If you have a dedicated fruiting room, it does not really matter if you get mold cause it does not spread to healthy tubs and you can install a carbon filter to reduce the spore count if you wish.
  On the other hand if you fruit where you have your SAB or flowhood, you are adding a contamination factor for your future grows


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Offlinewatsuu
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Re: Cultivation General Discussion [Re: feldman114]
    #26466903 - 02/03/20 02:18 PM (3 years, 11 months ago)

Quote:

feldman114 said:
Quote:

watsuu said:
Well, if my syringes are contaminated I hope i can atleast get something out of this shoebox.
I do have some mycelium currently growing on agar so at least I got that going on.




Syringes are never clean, that’s why we “clean” them up on agar.
They’re good for cakes and such, but not for stuff you’re planning to spawn to bulk.

This is coming from an ex-agar denier lol...thought I had clean spawn for months, didn’t get why people don’t use MS->grain, but it only looked clean. Agar only looks complicated btw - it’s the easiest step of a grow.



Only thing about agar is it pretty much needs a pressure cooker. Current situation is that i dont have one and dont really have room for one either. Which is why im running uncle bens pre cooked rice and the agar i got was pre poured petri dishes i got from a supplier.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: watsuu]
    #26466920 - 02/03/20 02:28 PM (3 years, 11 months ago)

You can steam agar. Liquids sterilize incredibly easily.


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: watsuu]
    #26466924 - 02/03/20 02:33 PM (3 years, 11 months ago)

Quote:

watsuu said:
Quote:

feldman114 said:
Quote:

watsuu said:
Well, if my syringes are contaminated I hope i can atleast get something out of this shoebox.
I do have some mycelium currently growing on agar so at least I got that going on.




Syringes are never clean, that’s why we “clean” them up on agar.
They’re good for cakes and such, but not for stuff you’re planning to spawn to bulk.

This is coming from an ex-agar denier lol...thought I had clean spawn for months, didn’t get why people don’t use MS->grain, but it only looked clean. Agar only looks complicated btw - it’s the easiest step of a grow.



Only thing about agar is it pretty much needs a pressure cooker. Current situation is that i dont have one and dont really have room for one either. Which is why im running uncle bens pre cooked rice and the agar i got was pre poured petri dishes i got from a supplier.




You are mistaken, my friend. Do you have a pot with a tight-fitting lid? A rice cooker? An instapot?

The smaller the container, the easier it is to sterilize. I have a pc, but I only run it for 15 min when sterilizing agar no-pour plates, compared to 2h for grains.


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Offlinewatsuu
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Re: Cultivation General Discussion [Re: mushboy]
    #26466926 - 02/03/20 02:34 PM (3 years, 11 months ago)

Quote:

mushboy said:
You can steam agar. Liquids sterilize incredibly easily.



Oh, is that right? I was under the impression that it needed a pc.
Interesting interesting. Something like make the agar mix in a bottle and steam for couple of hours in a pot?

Time to go look at agar teks i guess :rockon:
Thanks for the info


Edited by watsuu (02/03/20 02:35 PM)


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: watsuu]
    #26466939 - 02/03/20 02:39 PM (3 years, 11 months ago)

Mix the agar and steam for 30min? I dunno it's been a long time since I steamed agar no pours. Maybe 45 min?


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Offlinewatsuu
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Re: Cultivation General Discussion [Re: mushboy]
    #26466943 - 02/03/20 02:41 PM (3 years, 11 months ago)

Quote:

mushboy said:
Mix the agar and steam for 30min? I dunno it's been a long time since I steamed agar no pours. Maybe 45 min?



Nice, thanks for that! Even if its like 1 hour it wouldnt be bad.
Time to go looking for some agar mix and other stuff i guess.


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: watsuu]
    #26466945 - 02/03/20 02:43 PM (3 years, 11 months ago)

Quote:

watsuu said:
Quote:

mushboy said:
Mix the agar and steam for 30min? I dunno it's been a long time since I steamed agar no pours. Maybe 45 min?



Nice, thanks for that! Even if its like 1 hour it wouldnt be bad.
Time to go looking for some agar mix and other stuff i guess.



:poast::thatsinteresting:


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