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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: Jooby]
    #26452267 - 01/25/20 05:18 PM (4 years, 5 days ago)

So I'm looking to make a mass of spore prints, but none of the teks I found really utilized a flow hood during the process. I think foil comes off the roll sterile enough for my taste, so I'm considering modifying this tek to suit my needs: https://www.shroomery.org/forums/showflat.php/Number/24732724/fpart/all/vc/1

I don't have any experience making prints really, so a sanity check would be nice :blazed:

And some feedback would be great before I waste my time and spores. So I'm planning on just turning on my hood (after the usual prep routine), laying down my baking tray and a sheet of foil over it (after setting aside the first dirty foot or so - otherwise not really sterilizing anything in the oven or anything like that), place my sanitized cookie rack on top, place caps on cookie rack, place baking tray with all this on it into an appropriately sized sterilite, and close the lid and place in a draft free room.

Once prints dry / after 24 hours, get in front of hood and pull out baking rack with everything on it. Remove baking rack and discard another foot of foil. Grab new piece of foil, and (this is where it gets a little expirimental) carefully place it over your prints (it'd probably be good to measure my original piece and copy that measurement here for exactness, and on a similar note, since your prints are now covered and not visible, it'd probably be a good idea to have consistent spacing for where you place them (say every 4 inches, on center)). Draw some lines that correspond to where you should cut, and cut through both layers of foil throughtout your grid of prints. Now fold and put into ziploc, etc etc..

Also, ofc you could try to squeeze a second print out of the caps by transferring the cookie rack to a new foil covered baking tray within a sterilite (all in front of the hood of course).

Good idea? Or is this too lazy / non-sterile? The only thing I'd be doing differently, really (I think at least), is using a new piece of foil to just place right over everything, rather than folding it all beforehand.


Edited by junk_f00d (01/25/20 05:35 PM)


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452315 - 01/25/20 05:47 PM (4 years, 5 days ago)

Just cut them out and fold the foil onto itself.  Leave enough room for this when you go to print.  You don't need a flow hood just a cover for the caps while they drop spores, ideally a SAB but any sort of container can be used.  You want the spores to drop straight down with as little of the caps touching the foil as possible so you don't want any air flow, just still air under and around the caps.


:cookiemonster:


--------------------

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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #26452394 - 01/25/20 06:40 PM (4 years, 5 days ago)

That sounds good too. But FWIW, I wasn't talking about leaving these in front of a hood the whole time, just during placement and moving (moving into a container like you mention).

My biggest concern is rather than cutting and folding a bunch of pieces, would it be a bad idea necassarily to just place a new, fresh piece of foil over all my prints (and then cutting and folding). It would be more efficient (and perhaps more sterile? since your hands don't glide directly over the print at all while cutting..) this way I imagine (in terms of time and space), just placing a new sheet over the foil, cutting your rectangels, then just fold the edges inward at the edges, but I'm not sure if putting foil straight off the roll onto new prints is gonna give me a bad time.


Edited by junk_f00d (01/25/20 06:42 PM)


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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452396 - 01/25/20 06:41 PM (4 years, 5 days ago)

That would be my thought\fear. That a FH would blow away most of your spores as these are released.

I think that is why there are no FH teks. You need still air for them to fall straight down and create the print.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (01/25/20 06:43 PM)


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: Randalf the Grey]
    #26452403 - 01/25/20 06:44 PM (4 years, 5 days ago)

You guys have not read what I've written, lol. I am NOT talking about letting the spores drop while I run my hood, waste electricity and clog up my work space. Just during the transfers (cap to rack over foil, cookie rack to container). If you have the patience and kindness, please reread what I said about the double foil layer towards the end. I just want to know, essentially, if rather than precutting or folding, if it's acceptable to just place a NEW sheet of foil over the prints, then cut and fold everything.

That way I get way more prints per sterilite container and on my hood table at once, and save some time cutting and folding a buncha little guys.

I assume this is fine, but like I said haven't printed much, maybe non-oven-sterilized foil really sucks that much, for example (doubt it though).


Edited by junk_f00d (01/25/20 06:49 PM)


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OfflineSamskara92
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452410 - 01/25/20 06:48 PM (4 years, 5 days ago)

I've recieved a few prints that were wrapped in a secondary sheet foil. :shrug:

Cleaned up just fine.


--------------------
:jellyfish: We're all just Motion in the Dust:jellyfish:


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: Samskara92]
    #26452414 - 01/25/20 06:53 PM (4 years, 5 days ago)

I've gotten them like that before too, not sure if that implies they were made this way though but :shrug: I'm running my first test upstairs right now.. Standard method on one batch and the double foil layer on another. Probably be fine but I'm thinking about making some syrings soon (first timer), so cleanliness is super important

thanks!


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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452417 - 01/25/20 06:54 PM (4 years, 5 days ago)

Sorry. I did indeed misread your first post and your second post was made while I was commenting.
If you are taking uncovered printing caps from FH to container, there is risk. In the process of opening and closing container you are also creating air currents no matter how much you sanitized it and no matter how clean your caps ad foil and rack is, you could be contaming it all by bringing in "dirty" air . If you leave caps to print in a sealed container, you will need to remover caps before prints will dry; they release a lot of moisture.
Covering caps with a clean foil tent with help if you just have to transport them and put them in a container.
It sounds as though you are using s FH to start, then moving to what is essentially and SAB, but without some of the properties that make SABs great, like not opening a lid.
I always get 2 prints per cap. There is a two step printing Tek here somewhere. Print for a few hours then move to clean foil and let go over night.
First print will (likely) contain most of the contams that were present of caps, and second print is cleaner; better suited for syringes.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (01/25/20 06:58 PM)


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OfflineSamskara92
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452431 - 01/25/20 07:01 PM (4 years, 5 days ago)

Quote:

junk_f00d said:
I've gotten them like that before too, not sure if that implies they were made this way though but :shrug: I'm running my first test upstairs right now.. Standard method on one batch and the double foil layer on another. Probably be fine but I'm thinking about making some syrings soon (first timer), so cleanliness is super important

thanks!




Well, if you end up with several, feel free to send one my way and I'll test the cleanliness of her!

:bender:


--------------------
:jellyfish: We're all just Motion in the Dust:jellyfish:


LAGM 2020 Grow Log - KSSS
CBK's Proper Surface Moisture/ FAE Tek


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Offlinefootpath
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Re: Cultivation General Discussion [Re: Samskara92]
    #26452449 - 01/25/20 07:12 PM (4 years, 5 days ago)

Regardless, they're growing in unsterile conditions.
While it's great to keep shit out of your prints, going balls out on making pristine prints seems like more of a headache than it's worth, especially when you're expecting to clean it up anyway.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: footpath]
    #26452453 - 01/25/20 07:16 PM (4 years, 5 days ago)

not if you wanna make a syringe


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
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https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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Offlinejdawg333
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Re: Cultivation General Discussion [Re: TedsDead]
    #26452456 - 01/25/20 07:18 PM (4 years, 5 days ago)

I'm considering just not cutting any corners with Bod's Easy AF tek for a first grow. That is, use oats/grain, use a PC, use agar, and a tub. Think it would be better to just do PF tek instead, even with qt jars?


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: jdawg333]
    #26452463 - 01/25/20 07:24 PM (4 years, 5 days ago)

Pf tek is the worst.

A quart of grain to coir in a shoebox is way easier and yields more.

Just put a top layer of coir when you spawn the tub and it’s so much more forgiving of conditions while you learn what you’re doing.


--------------------
LAGM2020


Edited by A.k.a (01/25/20 07:26 PM)


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26452465 - 01/25/20 07:25 PM (4 years, 5 days ago)

What's that about the syringe?

Quote:


If you leave caps to print in a sealed container, you will need to remover caps before prints will dry; they release a lot of moisture.





Well, if the container has a sealed lid, it's essentially the same function for this purpose as a proper SAB right? And yeah, I like the sound of double printing anyway; more prints + lower contams on the second one :^)


Quote:


Well, if you end up with several, feel free to send one my way and I'll test the cleanliness of her!




ahah, we'll see! I got to make sure they're reasonably clean first, as a noob and all.


Quote:


Regardless, they're growing in unsterile conditions.
While it's great to keep shit out of your prints, going balls out on making pristine prints seems like more of a headache than it's worth, especially when you're expecting to clean it up anyway.





My thinking is about the same. If there's free wins, like just doing shit in front of my flowhood instead of in open air, I'll take those, but I'm not going to "sterilize my scissors" or anything. And baking foil for an hour seemed waaaaay over the top, imo.


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: jdawg333]
    #26452470 - 01/25/20 07:26 PM (4 years, 5 days ago)

PF tek is outdate imo.. I skipped it and went straight for bulk sub shoeboxes with MS grain spawn.
With that being said, PF tek will teach you patients and basic mushroom cultivation skills that will be useful for your futures grows...

Its up to you, just gotta start somewhere man! :shrug:


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: A.k.a]
    #26452472 - 01/25/20 07:28 PM (4 years, 5 days ago)

Quote:

A.k.a said:
Pf tek is the worst.

A quart of grain to coir in a shoebox is way easier and yields more.

Just put a top layer of coir when you spawn the tub and it’s so much more forgiving of conditions while you learn what you’re doing.





PF tek is the shit!!!  whats not to love?  syringe to cake, no PC!?.  it has so much potential in breeding as well.  in the beggining I did 1 pf grow before moving on to trays/tubs of manure/straw.  i would have been at a loss of info and experience if not for having done the pf 1st which is much less labor/cost intensive:shrug:  now yrs later I still refer to it all the time whenever the situation calls for it.  great for testing on a small scale!!!


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: TedsDead]
    #26452477 - 01/25/20 07:32 PM (4 years, 5 days ago)

Honestly I’ve been thinking about doing some 😂

Cronicr’s new pf tek thread with the boxes is dope.


--------------------
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LAGM2020


Edited by staytrippy420 (01/25/20 07:38 PM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: TedsDead]
    #26452481 - 01/25/20 07:35 PM (4 years, 5 days ago)

Lol no pc is the only reason I’d do it again.

It just takes so long with so much maintenance for a couple grams. It’ll definitely help you learn what conditions they like but that’s also a problem when you don’t know what you’re doing.

It’s just so much easier to mix up a shoebox and maybe mist it every couple days and end up with a half ounce even if you manage to mess things up.

By breeding you mean knocking it up with two syringes? I’ve never heard of a breeding application for them.


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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: A.k.a]
    #26452487 - 01/25/20 07:40 PM (4 years, 5 days ago)

I’ll do some... with the PC tho :lol:


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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: staytrippy420]
    #26452518 - 01/25/20 07:58 PM (4 years, 5 days ago)

@junk_f00d-The difference between a box with a lid and a SAB is that you are not removing a lid each time to enter the SAB. You can slide your arms\items into an SAB without creating much air current. Removing a lid, reaching in and leaving\removing items, replacing lid creating currents and draws outside air into box where it then falls onto your prints.
I am not trying to tell you this is impossible to get a useable print from, just pointing out critical control points.

The point of moving cap to second sheet a few hours into print is to limit potential contams to the first print. (Thus making second print better suited for syringes or times with cleaniness of print matters more, and first prints for agar and non-time sensitive works) If doing this in open air, or in a container with open air being drawn in, you are losing that benefit.
As far as the prints drying, I'm just saying that drying is a step. Make the print, remove caps, allow to dry, then cover print.
Here is the printing Tek I am referring to.
Bod's Proper Spore Printing Tek

I don't know about others, but I have not baked or "sterilized" my foil. That is just an extra step. And wiping with iso is counterproductive. As you said, just don't use first portion and use the inside side of the foil.
Not trying to stop you. I am trying to help you improve this idea.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (01/25/20 08:02 PM)


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