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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: feldman114] * 1
    #26446371 - 01/22/20 01:10 PM (4 years, 8 days ago)

The blob itself is not a genetic mutation it's a genetic trait, otherwise we would clone blobs and grow blobs. Blobs are just unorganized tertiary mycelium likely due to nutrition/ conditions which is why you don't keep seeing them through following flushes.
Contam resistance comes more with genus and species than strains, cubes in general will fruit in the face of contams and contams are often fairly slow to take a sub over where as something like semilanceata can have one green dot and it just shuts down or ganoderma will just eat most contams and shit it out for breakfast.
Pinning time can be a genetic trait , if it is consistently slow or fast that's what you got but just because it took long to pin the first time doesn't mean it will after it's cloned and vice versa but as seen in PE some do indeed take there time


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinefootpath
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Registered: 07/16/19
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Re: Cultivation General Discussion [Re: cronicr]
    #26446386 - 01/22/20 01:21 PM (4 years, 8 days ago)

Shouldn't it still be largely possible to breed in contam/disease resistance? At least as far as the potential of the species can go?
I'd assume many of these indoor axenically-grown cultures are at least varied, if not very much lacking, in the contam/disease resistance of their wild origin... no?


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: footpath] * 1
    #26446395 - 01/22/20 01:27 PM (4 years, 8 days ago)

The problem with that is the billions of contams and their ability to mutate quickly, as soon as you find a way so do they, prevention is and always will be key. Why focus on trying to get by with shitty technique when it's easy to just figure out where you went wring to start


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinefeldman114
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Registered: 09/06/19
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Re: Cultivation General Discussion [Re: cronicr]
    #26446398 - 01/22/20 01:28 PM (4 years, 8 days ago)

Quote:

cronicr said:
The blob itself is not a genetic mutation it's a genetic trait, otherwise we would clone blobs and grow blobs. Blobs are just unorganized tertiary mycelium likely due to nutrition/ conditions which is why you don't keep seeing them through following flushes.
Contam resistance comes more with genus and species than strains, cubes in general will fruit in the face of contams and contams are often fairly slow to take a sub over where as something like semilanceata can have one green dot and it just shuts down or ganoderma will just eat most contams and shit it out for breakfast.
Pinning time can be a genetic trait , if it is consistently slow or fast that's what you got but just because it took long to pin the first time doesn't mean it will after it's cloned and vice versa but as seen in PE some do indeed take there time




I really appreciate you taking the time to chew this through for me....gulp lol
@jbgtaa same to you, good sir.

Guess it’s back to the drawing board. This hobby man...just when you think you know a thing or 2, turns out you don’t know shit:unwanted:


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Offlinefootpath
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Re: Cultivation General Discussion [Re: cronicr]
    #26446404 - 01/22/20 01:31 PM (4 years, 8 days ago)

Understood.
It'd just be interesting strive to get it all as a possibly futile breeding project.
A fast-growing, clustering, potent, disease-resistant UFO on a medium-high stalk...

A man can dream.


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Offlinespiritlands
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Re: Cultivation General Discussion [Re: feldman114]
    #26446418 - 01/22/20 01:38 PM (4 years, 8 days ago)

Quote:

feldman114 said:

Guess it’s back to the drawing board. This hobby man...just when you think you know a thing or 2, turns out you don’t know shit:unwanted:




Ya but that's the best part, there's always something new to learn, new challenges to overcome and everything just gets easier.
:tryingnottodie:


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OfflinePrimalSoup
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Re: Cultivation General Discussion [Re: Failboat] * 1
    #26446444 - 01/22/20 01:56 PM (4 years, 8 days ago)

Cloning rules.

Quote:

Quirkmeister92 said:
You can take your clone samples, grow em out then eat a real dose to decide which to keep if you're really about selecting favorites.




Yeah what I'm doing but with small doses due to time.  These are the best so far of four I've compared:



They also have the cool pheno and make nice clusters which makes harvest easier. :solidnod:

There's a few left to try in this trial.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: PrimalSoup]
    #26446454 - 01/22/20 02:07 PM (4 years, 8 days ago)

Is there any benefit to pouring a few mls of sterile water into spawn jars that are too dry??

They’re growing but slowly so I’m thinking it might help to add some in the SAB before the 30% shake.

If it was one or two I’d just leave it alone but its like 90% of the ones I made over three days of prepping and pcing and they’re already knocked up.


I don’t know if the water needs to be soaked/cooked in or if just adding more will help.


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LAGM2020


Edited by A.k.a (01/22/20 02:09 PM)


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: A.k.a]
    #26446467 - 01/22/20 02:14 PM (4 years, 8 days ago)

You sure that’s the only explanation for thin-looking myc? I swear I remember someone saying it can be a sign of oversaturated grains as well.

On the up side, if you use too-dry grain jars as masters for g2g, they take off really fast in receiving jars. So I’d imagine they’ll colonize coir pretty fast as well.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: feldman114]
    #26446525 - 01/22/20 02:56 PM (4 years, 8 days ago)

Def not over saturated, usually I just soaked them them scooped a strainer full from the bucket and let it drip for 15-30 mins and they worked good but it was always wet and I’m trying to learn how to pick out bacteria wet from just water.

So this time after doing my normal routine I dumped them onto a table covered in paper towels then pressed more paper towels on top until they stopped leaving wet marks.

I’ve read other people do this but it looks like they normally heat and simmer the seeds too so that’s gotta be the difference.


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LAGM2020


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Offlinefeldman114
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Re: Cultivation General Discussion [Re: A.k.a]
    #26446546 - 01/22/20 03:09 PM (4 years, 8 days ago)

I soak my WBS, rinse with hot water and dry on an old towel, as you described.
Mine came out dry when I did a 12-hour soak. 24-36hrs does the job perfectly though.

What if you prepare some half-full jars of grain on the wet side, then transfer the partially colonized grain to them? It’s a risk but seems less sketchy than injecting water into the dry jars.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: feldman114]
    #26446677 - 01/22/20 04:14 PM (4 years, 8 days ago)

That’s another thing I was considering, I wanted 4 quarts of one kind but two look questionable so I was thinking about dumping the good ones into some partially filled jars prepped better.

The rest I was planning on shoeboxes with 1qt but I could always double up.
Guess it’s a good time to try out a few new things.


--------------------
LAGM2020


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: dmppb]
    #26446791 - 01/22/20 05:00 PM (4 years, 8 days ago)

Quote:

dmppb said:
isn't working with clones supposed to be a lot better ? isolates too but that takes a lot more work , right ?



I thnk yeah if your good at it and proceed in the appropriate fashion. It's really jst my personal preference, dude.
Our mush bros/sisters come up with all kinda amazing clone batches.
Hunting for an isolate (you may mean monoculture) is futile imo. Seems like a group of strains that simply make for a pretty plate.

Im on walkabout atm, but when i return to Babylon i plan to run new multispore cultures for every batch. Least amount of transfers possible.
I hear a lot of shit about syringe to grain, but one in 5 say it's not only simpler but they boast something like a 90% success rate. We shall see.


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26446937 - 01/22/20 06:31 PM (4 years, 8 days ago)

Syringe to grain can be great as long as you’re sure that you syringe is contaminate free. I’ve had amazing success with syringe to grain when I first started, and sometimes the diverse genetics make for a better flush. I do MS all the time, the only difference is I only do 1qt in a super small Tupperware and find clones to work from


--------------------
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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Feasoghorm] * 1
    #26446947 - 01/22/20 06:37 PM (4 years, 8 days ago)

Quote:

GypsyCurse said:
Quote:

dmppb said:
isn't working with clones supposed to be a lot better ? isolates too but that takes a lot more work , right ?



I thnk yeah if your good at it and proceed in the appropriate fashion. It's really jst my personal preference, dude.
Our mush bros/sisters come up with all kinda amazing clone batches.
Hunting for an isolate (you may mean monoculture) is futile imo. Seems like a group of strains that simply make for a pretty plate.

Im on walkabout atm, but when i return to Babylon i plan to run new multispore cultures for every batch. Least amount of transfers possible.
I hear a lot of shit about syringe to grain, but one in 5 say it's not only simpler but they boast something like a 90% success rate. We shall see.



People like to say it but you only ever see multiple tubs posted by those doing agar work, the only syringe posts we tend to see are " what's wrong with my jar" lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: cronicr]
    #26447000 - 01/22/20 07:05 PM (4 years, 8 days ago)

Well i forgot what the actual issue was. My first grows were cakes and that's syringe to grain. I thnk 2 out of 30 were contaminated so that's pretty damn good.


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26447013 - 01/22/20 07:13 PM (4 years, 8 days ago)

I have had a lot of success and failure with both ways, it really just depends on your goals, if you're going to make a hobby out of it you can't rely on a syringe


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: cronicr]
    #26447094 - 01/22/20 07:52 PM (4 years, 8 days ago)

Word
:jennajameson:


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26448120 - 01/23/20 12:28 PM (4 years, 7 days ago)

Figured i may get a faster response here. How much stronger is ape than PE?


--------------------
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OfflineTstone
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26448184 - 01/23/20 01:13 PM (4 years, 7 days ago)

Same, and I find APE  significant lower yeilds. Personally, I would work with PE, over APE any day of the week. APE really not a production culture, unless you spend the time, resources to find the perfect  culture. I scraped APE all together. As most vendors mention in their description" novalty".  For cube variety strength and yield, PE. However, potency isn't guaranteed in any myco work. Don't expect the the stars from a single plate or jar.

And if you've never cultivated before, especially if doing cakes, don't do any PE


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Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"


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