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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: FunGuyZ]
#23922974 - 12/13/16 02:55 PM (7 years, 1 month ago) |
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Shake the hell out of it man.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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axis_tech
Rollin' Stoned


Registered: 02/27/05
Posts: 294
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: AlCapone2k]
#23923011 - 12/13/16 03:05 PM (7 years, 1 month ago) |
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something to think of after you are done doing an ms injection shake and mix the jars up really well to distribute the spores. often times you will have enough spots germinating through out it you wont have to shake during colonization.
-------------------- takes a token' and keeps on smoken'
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: axis_tech]
#23923068 - 12/13/16 03:22 PM (7 years, 1 month ago) |
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I never did when I used to use just shoot spores in a grain jar (poor results), I was under the impression you should shake until 30%. I understand there are a shit ton of spores though so it doesnt seem possible for spores to become isolated and not germinate. The spores can germinate individually anyway and the monokaryotic myc would all meet up anyway...
Wow I just contradicted the fuck out of myself
Poor results are pretty much garuanteed.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: Kenetic]
#23923128 - 12/13/16 03:39 PM (7 years, 1 month ago) |
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So I just shook them up and as healthy as the mycelium may have looked on the outside of the jar, it doesn't look so healthy on the inside (after shaking and looking at the network). I had a suspicion that they were stalling and it looks like they have- not enough moisture in the grains. Conditions too dry. They also may have been fighting off contams because now when I take a whiff near the SHIP it does smell pretty awful. Oh well. Time to start over. Thanks again for everyones input.
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: Cultivation General Discussion [Re: FunGuyZ]
#23923141 - 12/13/16 03:43 PM (7 years, 1 month ago) |
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After shaking the grains look like they aren't colonized, is that what you are talking about? If so that's normal. shaking means hitting the jar against a rolled up phone book, side of a shoe ect. But If you have to bang the crap out of it to get it to break it up its bacterial.
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: NDStepp84]
#23923158 - 12/13/16 03:49 PM (7 years, 1 month ago) |
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Is it also bacterial if the rye breaks up easily but the part around the agar wedge is a bit sticky so it doesn't break up immediately?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: NDStepp84]
#23923164 - 12/13/16 03:50 PM (7 years, 1 month ago) |
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Quote:
NDStepp84 said:
After shaking the grains look like they aren't colonized, is that what you are talking about? If so that's normal. shaking means hitting the jar against a rolled up phone book, side of a shoe ect. But If you have to bang the crap out of it to get it to break it up its bacterial.
Should it smell bad though? It smells pretty awful.
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Greg
always learning



Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Cultivation General Discussion [Re: FunGuyZ]
#23923180 - 12/13/16 03:54 PM (7 years, 1 month ago) |
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Most definitely contaminated.
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axis_tech
Rollin' Stoned


Registered: 02/27/05
Posts: 294
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: Kenetic]
#23923207 - 12/13/16 04:10 PM (7 years, 1 month ago) |
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Quote:
kenetic said: I never did when I used to use just shoot spores in a grain jar (poor results), I was under the impression you should shake until 30%. I understand there are a shit ton of spores though so it doesnt seem possible for spores to become isolated and not germinate. The spores can germinate individually anyway and the monokaryotic myc would all meet up anyway...
Wow I just contradicted the fuck out of myself
Poor results are pretty much garuanteed.
i always used to shake maybe 30 minutes afterwards, after i cleaned everything up, and it would be similar in comparison to a jar that wasn't shaken and the spores were constrained to a small area of grain and you had to shake at the 30%.
as long as the spores are viable you wont hurt them you just mix them evenly across the surface area of the grain so you get an even germination through out the jar. it has nothing to do with the benefits of cloning or isolating but if you are that is when you want to wait to shake.
-------------------- takes a token' and keeps on smoken'
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: axis_tech] 2
#23923716 - 12/13/16 06:22 PM (7 years, 1 month ago) |
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I just finished my longest SAB session yet, I'm broken. On the left, the plates I nocced with spores and on top of the SAB the transfers I made. Thought about taking one sample only from each of the plates I nocced with spores... but I got a bit carried away in the end 

I'm so excited, can't wait to see how this project pans out!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: axis_tech]
#23923735 - 12/13/16 06:27 PM (7 years, 1 month ago) |
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Quote:
axis_tech said:
Quote:
kenetic said: I never did when I used to use just shoot spores in a grain jar (poor results), I was under the impression you should shake until 30%. I understand there are a shit ton of spores though so it doesnt seem possible for spores to become isolated and not germinate. The spores can germinate individually anyway and the monokaryotic myc would all meet up anyway...
Wow I just contradicted the fuck out of myself
Poor results are pretty much garuanteed.
i always used to shake maybe 30 minutes afterwards, after i cleaned everything up, and it would be similar in comparison to a jar that wasn't shaken and the spores were constrained to a small area of grain and you had to shake at the 30%.
as long as the spores are viable you wont hurt them you just mix them evenly across the surface area of the grain so you get an even germination through out the jar. it has nothing to do with the benefits of cloning or isolating but if you are that is when you want to wait to shake.
Dont shake till 30 it will tell ya if your inoculation was clean
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Josex]
#23923741 - 12/13/16 06:29 PM (7 years, 1 month ago) |
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Nice box!
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Josex]
#23923799 - 12/13/16 06:48 PM (7 years, 1 month ago) |
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That must be quite the fuckin project
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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axis_tech
Rollin' Stoned


Registered: 02/27/05
Posts: 294
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: cronicr]
#23923859 - 12/13/16 07:03 PM (7 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
axis_tech said:
Quote:
kenetic said: I never did when I used to use just shoot spores in a grain jar (poor results), I was under the impression you should shake until 30%. I understand there are a shit ton of spores though so it doesnt seem possible for spores to become isolated and not germinate. The spores can germinate individually anyway and the monokaryotic myc would all meet up anyway...
Wow I just contradicted the fuck out of myself
Poor results are pretty much garuanteed.
i always used to shake maybe 30 minutes afterwards, after i cleaned everything up, and it would be similar in comparison to a jar that wasn't shaken and the spores were constrained to a small area of grain and you had to shake at the 30%.
as long as the spores are viable you wont hurt them you just mix them evenly across the surface area of the grain so you get an even germination through out the jar. it has nothing to do with the benefits of cloning or isolating but if you are that is when you want to wait to shake.
Dont shake till 30 it will tell ya if your inoculation was clean
I don't see how shaking would effect this. If it's clean you have even distribution and if it's dirty it's fucked shaken or not.
-------------------- takes a token' and keeps on smoken'
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: axis_tech]
#23923873 - 12/13/16 07:08 PM (7 years, 1 month ago) |
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You will spot it sooner..there is no advantage to shaking.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hellboun1
Stranger

Registered: 11/30/16
Posts: 2
Last seen: 6 years, 10 months
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Re: Cultivation General Discussion [Re: cronicr]
#23923889 - 12/13/16 07:13 PM (7 years, 1 month ago) |
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I have a question do u fan and mist wen pinning and also wen da mushrooms r out or u only fan and mist till it pins den u stop I'm a little confused
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Cultivation General Discussion [Re: Hellboun1]
#23923910 - 12/13/16 07:17 PM (7 years, 1 month ago) |
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Mist and fan as needed through out the fruiting process.
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: Cultivation General Discussion [Re: MudaFuka] 1
#23923946 - 12/13/16 07:30 PM (7 years, 1 month ago) |
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U rly don't need 2 fan da chamber take care all dat, U can mist wyl da mashrooms R out but jus az needed not 2 much 2 close to dun may get waterlogged an da blotch but even den all good jus dry dem dry
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: NDStepp84]
#23923968 - 12/13/16 07:34 PM (7 years, 1 month ago) |
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Quote:
NDStepp84 said: but even den all good jus dry dem dry
 Damn
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Cultivation General Discussion [Re: NDStepp84]
#23923973 - 12/13/16 07:34 PM (7 years, 1 month ago) |
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How do you know he doesn't need to fan? Have you seen his set up? He might be using a SGFC.
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