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Offlinespiritlands
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Registered: 06/21/10
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Re: Cultivation General Discussion [Re: TeaforTwo] * 1
    #26423561 - 01/08/20 08:09 PM (4 years, 22 days ago)

My shmuvbox does just fine when I have to clean up a culture. I do try to get the transfers done before contams have sporelated but even when I don't catch it in time I just work on those last. Occasionally I will get a little trich maybe 1/20 plates but that happens sometimes even when I'm not working on dirty plates. I clean it and replace the bag after I work on anything contamed. I can typically be in and out of a pitri a handful of times with no contam. I don't have issues with contams blowing around I just turn the fan down. I save my t1 plates even after I take transfers and I can take more transfers as it grows. I don't recommend it but I get away with it more often than not.

This is one plate I continually took transfers from until the whole thing colonized.


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Germinating old spores progress page
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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: spiritlands]
    #26423571 - 01/08/20 08:18 PM (4 years, 22 days ago)

You know that you can keep them in the fridge to stall growth, right?


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Offlinespiritlands
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #26423582 - 01/08/20 08:28 PM (4 years, 22 days ago)

Ya but I haven't gotten a dedicated fridge yet. I transferred the rhizo sector in the first pic and kept harvesting that sector as it grew. The print was from 2012 and I needed to save all the tissue I could bc it wasn't easy to get growing and it's not a popular variety. It's x7x and a personal favorite, I'm trying to save my collection bc it's getting old and I've had some dead prints. I didn't want to slow the growth down.


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Germinating old spores progress page
how to calibrate your Martha



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Invisiblesh4d0ws
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Registered: 02/26/08
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26423588 - 01/08/20 08:33 PM (4 years, 22 days ago)

Quote:

p9hu7 said:
There are mold spores everywhere, they're inescapable. Opening a plate in your house will not noticeably increase the spore load in your house, flowhood or not.

You are literally covered in them as we speak.




I'm not talking about increasing the spore load in a house though. Strictly I'm talking about opening a contaminated plate in front of a flow, while you are working on multiple cultures and have tools around. The flow can push those spores onto all kinds of shit, your tools, etc. Making it easier to spread to the stuff you are working on.

I always thought that best practice is to work on anything that's moldy, is to do it in an SAB for those reasons.

Are you saying it doesn't mater?


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: sh4d0ws] * 2
    #26423596 - 01/08/20 08:39 PM (4 years, 22 days ago)

Yeah if and when it happens just spray your hood and clean your area up and run the hood for an hour or so


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Invisiblegizmo1
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26423627 - 01/08/20 09:16 PM (4 years, 22 days ago)

Quote:

jbgtaa said:
Hey y’all, does anyone have any ideas for liner material that isn’t trash bag? I know Saran Wrap won’t work because it’s cellulose, but how about like.... wax/parchment paper? I’ve cut my budget down hugely and my break is coming to an end soon, and I’m gonna go back to trying some more traditional and cost effective methods.



Painters tarps from dollar tree will do multiple monotubs and cost a dollar. I used to use them when I used liners. I'll probably go back to using them i have like 10 painter's tarps left and I dont remember for sure but I think one tarp would do 5+ monotubs.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: sh4d0ws] * 2
    #26423644 - 01/08/20 09:28 PM (4 years, 21 days ago)

Quote:

sh4d0ws said:
Are you saying it doesn't mater?




Yes, it doesn't really matter as long as you're not using a massively overtaken mold plate, I wouldn't even bother at that point.
If you're not using a SAB it's just a matter of being smart about it by dealing with it last without opening jars and whatnot after having dealt with it.

Like cron said, just sanitize and run the hood, gtg. Spores are in the environment by the boat load, just open your curtains on a sunny day and watch all of the airborne contaminants swirl around the room.

The spore load thing was mostly for Teafortwo.

@spiritlands just throw it in your actual fridge in ziploc freezer bags, better than having to let it grow out.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: sh4d0ws] * 1
    #26423809 - 01/09/20 12:37 AM (4 years, 21 days ago)

Quote:

sh4d0ws said:
I was working on this culture in front of my flowhood. I hope I didn't spread mold spores everywhere unintentionally

Fuck




I jst go in the lab room with,vacuum a wet bleachy cloth and mop and give it a once over, Vacuum suck the grill with clean small attachment and then wipe with iso.  Turn it on for half a day low and you should be fine


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Edmunter]
    #26423816 - 01/09/20 12:52 AM (4 years, 21 days ago)

Im traveling away for 5 weeks, do you think I should keep all my colonised jars in the fridge to avoid invitro grow or will I be fine?  Im going to fill every jar I own so I have 50 fully colonised jars on my return.


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Offlinejcm4620
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Re: Cultivation General Discussion [Re: Edmunter]
    #26423937 - 01/09/20 03:41 AM (4 years, 21 days ago)

5 weeks ya for sure!! if you dont have someone able enough to step in. then ya id stall growth on all my shit. 5 weeks is enough time u could even come back to whole room full of green and god knows what else. thats just askin for trouble and some invitro fruits would b at the very bottom of that list

just my opinion tho


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: jcm4620]
    #26423950 - 01/09/20 03:59 AM (4 years, 21 days ago)

Quote:

jcm4620 said:
5 weeks ya for sure!! if you dont have someone able enough to step in. then ya id stall growth on all my shit. 5 weeks is enough time u could even come back to whole room full of green and god knows what else. thats just askin for trouble and some invitro fruits would b at the very bottom of that list

just my opinion tho




Nah im at 95% at least success rate with my jars,its more about would they colonised ones be better in the fridge or ok on a shelf for 5-6 weeks. I have someone taking what monos I still have running though.


Edited by Edmunter (01/09/20 06:12 AM)


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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #26424069 - 01/09/20 07:26 AM (4 years, 21 days ago)

Ed, I stored jars for three months once in the fridge, fully colonised mind, came home spawned them straight away and had a couple of massive flushes from them so I’d say you’re all good brother :thumbup:


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Smoothcat] * 1
    #26424119 - 01/09/20 08:13 AM (4 years, 21 days ago)

:whathesaid:    you don't want them sitting on the shelf for 5 weeks.  stall that sheeit.    also IME  putting stuff in the fridge is a good way to get hidden con-tams to reveal themselves.  when you pull em out you'll know what was good and what was bad


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #26424133 - 01/09/20 08:23 AM (4 years, 21 days ago)

Quote:

Edmunter said:
...Vacuum suck the grill




Cover the face of your hood with cardboard, this will prevent dust and what not from settling on your fins while not in use.



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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26424143 - 01/09/20 08:30 AM (4 years, 21 days ago)

Any reason to not PC agar bottles and grain jars in the same run? I know there is a significant time difference so I guess real question is will PCing agar for 90 mins cause harm?
I've already started it so if there is, I'll just make more agar bottles. But seems pointless to PC grain, the let PC cool, the PC agar.


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Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
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OfflineFailboat
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Re: Cultivation General Discussion [Re: Randalf the Grey] * 1
    #26424149 - 01/09/20 08:34 AM (4 years, 21 days ago)

I ran a media bottle with grain once. It discolored the print on the bottle.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #26424150 - 01/09/20 08:37 AM (4 years, 21 days ago)

lysol works well also.    at this commercial orchid farm I visited they sprayed their hoods down with ethanol before every use.  seemed over kill to me but guess it works:shrug:


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Randalf the Grey] * 1
    #26424153 - 01/09/20 08:40 AM (4 years, 21 days ago)

Shouldn't be an issue other than it will darken the agar and possible boil over, it won't ruin it though.

I have a smaller pc for running agar while I do grains. Super convenient but may be prohibitively expensive to buy solely for that purpose.



Quote:

TedsDead said:
lysol works well also.    at this commercial orchid farm I visited they sprayed their hoods down with ethanol before every use.  seemed over kill to me but guess it works:shrug:




I used to spray mine with lysol, haven't done that in ages and it hasn't made a difference.


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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26424160 - 01/09/20 08:45 AM (4 years, 21 days ago)

Meh, if that's the worst that happens, I'm good. Honestly, even if it comes out horribly caramelized and useless it's not that big a deal. It means I lost 15 g of agar between the two bottles. And still have to run two PC cycles. If it works low, I save myself about 5 hours or so

I actually do have a smaller PC but it is currently full of poppy tea because the pot I use for poppy tea was full of chili. Haha. Technically I have 3, but try to only use my instant pot for food. I don't care, but my gf feels weird about feeding her son food that was cooked in my "drug equipment" as she calls it.


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Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (01/09/20 08:48 AM)


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #26424163 - 01/09/20 08:46 AM (4 years, 21 days ago)

I used to spray my hood too but now i just let it run for a solid hour
before starting work. No issues since stopping the lysol spray a couple years ago.

I imagine most if not all the spores, that will detach from the filter face, do
so within that hour. But as a precaution I also put a piece of cardboard in front of
mine when not in use


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