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guyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
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Re: Cultivation General Discussion [Re: filthyknees]
#23604158 - 09/02/16 10:02 AM (7 years, 4 months ago) |
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i don't understand "cut a block" of 10 transfers?
i usually find the best growth, or cleanest areas, and then take a little wedge to a plate. usually one or two wedges per desired area.
are you talking about expanding 1 plate to 10 maybe?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: guyute22]
#23604189 - 09/02/16 10:15 AM (7 years, 4 months ago) |
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Yes. Cutting a rectangle then many squares within it, then transferring the squares.
Not when isolating, sorry if I was unclear but I'm not talking about isolating, just expanding already isolated growths to make masters with.
Like I had ten plates with growth that was ready to go already, expanded for making many (5-20x) plates of each for grain masters from each plate.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
Edited by filthyknees (09/02/16 10:19 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,807
Loc: Canada
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Re: Cultivation General Discussion [Re: filthyknees]
#23604218 - 09/02/16 10:26 AM (7 years, 4 months ago) |
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That sounds like a great way to expand plates.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Pastywhyte]
#23604410 - 09/02/16 11:37 AM (7 years, 4 months ago) |
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you get the same amount of wedges,same size and everything just by fewer cuts like tic-tac or pizza slices etc? but for cleaning on agar and not inocing grains?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: spacechildo]
#23604417 - 09/02/16 11:39 AM (7 years, 4 months ago) |
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Words aren't doing it for me, gonna have to see video of this or something to properly understand.
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Fractaliopsybe
⊰⚜⊱



Registered: 06/21/15
Posts: 1,942
Loc: ☮☣✯
Last seen: 7 years, 11 days
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Re: Cultivation General Discussion [Re: Inocuole]
#23604438 - 09/02/16 11:47 AM (7 years, 4 months ago) |
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Quote:
Inocuole said: Let's play "Who has the most BOM points?" https://www.shroomery.org/forums/banomatic.php Total Points: 9
hahah that's cool! Funny how I have nothing.....^^ ^ ---------------------You have been entered in the ban lottery.--------------------------- You might now be banned for absolutely no reason. Congratulations, what a smart thing to do. You should truly be proud. Current ban odds are 1:7 (14.3%) These people have entered the ban lottery this week: Society, JustAnotherAsshole, 1234go, Chemical Addiction, Kilgore Trout, Revok, Buck513 ^-------------------------Well... that's seems semi-Familiar.... --------------------^ Quote:
Fractaliopsybe said: Shalom Everyone. Does any know of any1 Fruiting P.subs with the help of a fridge?
 Alloy tray with glad wrap atop, Trialling 8-16hrs in 5c* (RH?-TBA) then take out and put in 13c @ 80-94RH tub and play with the "massaging" of temp changes in and close to out the right conditions. Is what I'm hoping will help boost it so I can converge this in to a consistent indoor TEK, Applied with to double layer SGFC tubs or bucket. Which is in proper trials now, So I will keep the TEK that I'm attempting in consolidation and Break it down before releasing and can wait to present successful results* (finger-crossed) I've had some success already.. just not that well.. As few pins pop up but has a 50/50 chance of Growing or aborting. *hints my trials of this as, Lots of people just do YEARLY/ season woodlover grows from what ive seen. but none are indoor.
Wish me luck and Suggestions are appreciated.

Also With the Regeneration of P sub cakes as they can well.. Regenerate... DoctorGhosty (TI) Is trying to help but I seem to be the only one trying to bring p. subs fully indoors...?
Any Links peeps!!!?!?!?!?!? Cheers in advance
-------------------- Psilocybe Cubensis. Panaeolus Cyanescens. Psilocybe Subaeruginosa. Dimethyltryptamine.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: spacechildo]
#23604632 - 09/02/16 01:14 PM (7 years, 4 months ago) |
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Yes fewer cuts and thus much less time with the plate open.
Yes you can imagine tic tac toe but i just use a rectangle. With one scalpel sterilization i can have 10 transfer pieces made.
Best i can do on a picture, i just googled rectangle made of squares.

If i was doing twenty transfers from one plate id cut two rectangles on opposite sides of the plate, cut the rectangles into ten pieces each muchcfaster than twenty individual square cuts.
Since the squares are precut, after the grid is cut the plate iz open for literallyless than one seconds to retrieve each of the next squares to transfer
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,807
Loc: Canada
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Re: Cultivation General Discussion [Re: filthyknees]
#23604648 - 09/02/16 01:19 PM (7 years, 4 months ago) |
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Exactly what I pictured. I like it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Pastywhyte]
#23604652 - 09/02/16 01:20 PM (7 years, 4 months ago) |
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So these are like, little baby squares right? Or do you have huge dishes or something? Biggest I have is the wide mouth half pints I use. Guessing I could cut out like 24 or so little squares pretty easily out of each.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: Inocuole]
#23604663 - 09/02/16 01:26 PM (7 years, 4 months ago) |
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Easily. Yep. I use standard dishes or slightly smaller.
Each square is about two grains of rice.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Cultivation General Discussion [Re: filthyknees]
#23604671 - 09/02/16 01:28 PM (7 years, 4 months ago) |
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People haven't done this before? I haven't read much about agar but my understanding was that this was common practice?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Terpfreak]
#23604703 - 09/02/16 01:38 PM (7 years, 4 months ago) |
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Well I like bigger wedges so... Common practice isn't necessarily a thing based on a myriad of preferences.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Cultivation General Discussion [Re: Inocuole]
#23604716 - 09/02/16 01:41 PM (7 years, 4 months ago) |
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Youre very right about that. Good to hear different ideas and see the results , thanks for the input -whoever the OP of this topic is.
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Boomertown
Psychonaut



Registered: 06/21/15
Posts: 183
Last seen: 5 years, 4 months
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Re: Cultivation General Discussion [Re: Terpfreak]
#23605211 - 09/02/16 03:50 PM (7 years, 4 months ago) |
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Wouldn't those cuts have a higher chance of contaminating due to so much handling, even if its done in a SAB or in front of a flow hood? I mean when I transfer from plate to plate, I do it as quick as possible. I feel like what you're explaining, you would have your master plate exposed a lot longer then just one transfer out of a plate?
Anyways if you did a video of you doing this, that would be fantastic.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Cultivation General Discussion [Re: Boomertown]
#23605221 - 09/02/16 03:54 PM (7 years, 4 months ago) |
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Anything with more handling equals more contam chances. But more plates and jars also means higher chances of success so it's really what you wanna wager would be best for you, as inocuole reminded me earlier.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Terpfreak] 1
#23605233 - 09/02/16 03:57 PM (7 years, 4 months ago) |
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It's going to depend on how refined your technique is. If you rely on exposing your work for very short amounts of time as a way to keep your success in line, then changing that will require you to change some other things as well to accommodate. At one point most facets of your technique will be refined enough that you can determine the vectors that cause you the most issues personally and avoid them completely by using your own compilation of techniques. Most people's strengths are stronger than their weaknesses are weak. Most anyway. But yeah, no matter what you read a lot of it is still going to involve playing it by ear, which thankfully, is one of the most effective life skills to brush up on.
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Boomertown
Psychonaut



Registered: 06/21/15
Posts: 183
Last seen: 5 years, 4 months
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Re: Cultivation General Discussion [Re: Inocuole]
#23605267 - 09/02/16 04:09 PM (7 years, 4 months ago) |
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Well from my own experience, when I take multiple transfers from my master plate in a single go at it. I tend to have a clean first chunk and the other ones tend to get bacterial.
Because I noticed that, when I do multiple transfers now I cover, re- heat scalpel, make next cut, then repeat.
So you wouldn't re heat your cutting device every time you make a small chunk out of your rectangle? What if half way through your rectangle one chuck of it has an edge of bacteria on it which is now on your scalpel, which would in turn cause the rest of your cuts to have that bacterium on it. Or am I just over thinking this?
Edited by Boomertown (09/02/16 04:11 PM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: Boomertown]
#23605424 - 09/02/16 04:57 PM (7 years, 4 months ago) |
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I don't think you're over thinking. I would only use that method when already working with all clean looking growth: expansion & not nessecarily isolation (although isolation will happen).
If I indeed had a clean culture on the plate I do not see how making a larger dimension cutting would introduce bacteria
I'll post an example the next time I'm expanding. To who wondered if it was common practice: that is also my wonder & why I posted
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: filthyknees]
#23605570 - 09/02/16 05:54 PM (7 years, 4 months ago) |
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Are there inoculation devices/knives that have a mechanism sort of like an ice cream scoop? Where you can push a little lever and have something ejected off the tip? If not, is there a practical reason that isn't a thing?
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 1 day
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Re: Cultivation General Discussion [Re: Inocuole]
#23605583 - 09/02/16 05:58 PM (7 years, 4 months ago) |
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Quote:
Inocuole said: Are there inoculation devices/knives that have a mechanism sort of like an ice cream scoop? Where you can push a little lever and have something ejected off the tip? If not, is there a practical reason that isn't a thing?
Believe it or not I've thought of the exact same thing. It would be so handy to have a device like that.
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