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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26411275 - 01/01/20 05:27 PM (4 years, 29 days ago) |
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Quote:
GypsyCurse said: 2020...Gonna be a good year, fam!

A GREAT year!
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Cultivation General Discussion [Re: gizmo1]
#26411279 - 01/01/20 05:29 PM (4 years, 29 days ago) |
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I don't understand why: I pour 60 plates from 3 different media bottles and have no issues... like: what's the purpose? So you can sip a spot of tea whilst the agar takes a bath?
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
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Re: Cultivation General Discussion [Re: Drboomer]
#26411282 - 01/01/20 05:31 PM (4 years, 29 days ago) |
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Quote:
Drboomer said: Has anyone ever used one of those cheap sous vide sticks to hold temperatures on a media bottle for pouring agar?
I used to have a spare one laying around. Not sure where it is now though. Good idea for sure. Cheap one should be fine. A fluctuation of even 10°F should be fine and even the cheap ones are more accurate than that.
Quote:
Caps McGee said: I don't understand why: I pour 60 plates from 3 different media bottles and have no issues... like: what's the purpose? So you can sip a spot of tea whilst the agar takes a bath?
I used to just heat water to 120, then add hot media bottles and wait for water to return to 120. This was when I first started agar and had read it was a good idea. At the time, it was. I was too slow and unsure of my movements to pour all the agar either before it cooled too much or without starting to burn while it was too hot. More recently, I am better and more fluid at my lab work, but I have also started PCing then reheating agar in microwave so getting right temp is WAY easier, so no need for hot holding.
Edited by Randalf the Grey (01/01/20 05:37 PM)
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A.k.a
Stranger


Registered: 10/27/19
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Is sous vide a thermometer?
You could prob get one of those sticker ones they use in fish tanks and stuff and slap it on the bottle. Somebody’s gotta make one that goes up to 200f
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LAGM2020     
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
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Re: Cultivation General Discussion [Re: A.k.a]
#26411309 - 01/01/20 05:40 PM (4 years, 29 days ago) |
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Quote:
A.k.a said: Is sous vide a thermometer?
You could prob get one of those sticker ones they use in fish tanks and stuff and slap it on the bottle. Somebody’s gotta make one that goes up to 200f
Sous Vide is an temp controlled immersion circulator. You set a temp and it heats and holds the water within 0.1° while circulating it to keep temp even. French for "under pressure\vacuum" it is a way of cooking food that has been cryovacced, or vacuum sealed, at very controled temps and times. The perfect mid rare steak or poached eggs or salmon fillets.
Edited by Randalf the Grey (01/01/20 05:42 PM)
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Drboomer
The lord magnificent



Registered: 09/22/19
Posts: 957
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Re: Cultivation General Discussion [Re: Caps McGee]
#26411402 - 01/01/20 06:51 PM (4 years, 29 days ago) |
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Quote:
Caps McGee said: I don't understand why: I pour 60 plates from 3 different media bottles and have no issues... like: what's the purpose? So you can sip a spot of tea whilst the agar takes a bath?
I was thinking more for pre sanitized bottles. I never get to cook it and pour on the same day. Just pop it in a bath set at the right temp while dinners going and keep it at temp till you get set up to pour even if it takes a couple hours before you are ready.
Quote:
gizmo1 said: That will work fine for a water bath for agar. I dont have a sous vide yet but I'm definitely going to buy one eventually. They are pretty cool I want to cook a steak with one.
Right! I had a sous vide steak and it was super tender and juicy. Salmon fillets with seasoned butter were great aswell. I want one to cook with so maybe it would make a great water bath. They have come down to below 50 bucks now.
Edited by Drboomer (01/01/20 06:57 PM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Quote:
Randalf the Grey said:
Quote:
A.k.a said: Is sous vide a thermometer?
You could prob get one of those sticker ones they use in fish tanks and stuff and slap it on the bottle. Somebody’s gotta make one that goes up to 200f
Sous Vide is an temp controlled immersion circulator. You set a temp and it heats and holds the water within 0.1° while circulating it to keep temp even.
Ahha...i was like wtf you guys, i jst tape/saran wrap a candy therm on the side of the bottle after it comes outta the PC. Wait till it gets to 117F.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26411419 - 01/01/20 07:14 PM (4 years, 29 days ago) |
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Quote:
GypsyCurse said:
Quote:
Randalf the Grey said:
Quote:
A.k.a said: Is sous vide a thermometer?
You could prob get one of those sticker ones they use in fish tanks and stuff and slap it on the bottle. Somebody’s gotta make one that goes up to 200f
Sous Vide is an temp controlled immersion circulator. You set a temp and it heats and holds the water within 0.1° while circulating it to keep temp even.
Ahha...i was like wtf you guys, i jst tape/saran wrap a candy therm on the side of the bottle after it comes outta the PC. Wait till it gets to 117F.
I never do enough plates and have gotten smooth enough to not need to hot hold them. However, the allure for me is that you could potentially hold at low enough temp that it is still fluid enough to pour while absolutely minimizing condensation. All plates would be poured at same temp instead of a range of temps as it cools and reduce the amount of time I have to let them sit for condensation to dissipate. Not a huge deal, but would be more consistent and visually pleasing (for me) instead of gradient of condensation across my pours.
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iEnter
Stranger


Registered: 03/17/16
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Quote:
shroomgirl645 said: got a few jars that are scheduled for harvest today, let's hope that at least one isn't contaminated
Congrats
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A.k.a
Stranger


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Re: Cultivation General Discussion [Re: iEnter]
#26411491 - 01/01/20 08:07 PM (4 years, 29 days ago) |
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I hate condensation so much.
I have a thermal gun thing to check the agar and I would start to pour around 125 but still get crazy condensation and never had a problem with the agar getting thick while pouring.
So this last time when it said 120 I moved the thermometer from a foot away to an inch away and it hit 150ish.
Hopefully that discovery will end my problems.
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LAGM2020     
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Randalf the Grey
Woodland Creature


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Re: Cultivation General Discussion [Re: A.k.a]
#26411496 - 01/01/20 08:12 PM (4 years, 29 days ago) |
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I feel like I've gotten better results temping the jar by hand than I ever did with a water bath or thermometer. I would love to try it held perfectly at 110 and go from there. The last plates I pour always clear up quickly and beautifully so I know I'm close to the sweet spot, I just want it to be constant.purely for personal preference and sanity though. Totally not needed.
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spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
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Ok so a while back I had some Redboy that grew a bunch of mutants but didn't seem bacterial. I also had some yeast issues in other jars but I got rid of it. I took a clone from a good cluster and it's colonizing a pint jar but I see a tiny bit of yeast. I kind of suspect that the mutation was it fighting yeast the whole time.
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Yesum
Furry as Fuc



Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Quote:
Randalf the Grey said: I feel like I've gotten better results temping the jar by hand than I ever did with a water bath or thermometer. I would love to try it held perfectly at 110 and go from there. The last plates I pour always clear up quickly and beautifully so I know I'm close to the sweet spot, I just want it to be constant.purely for personal preference and sanity though. Totally not needed.
That's what I'm saying. Seems like a awful lot of bolagna for nothing. . To each there own. It's pretty simple. If u can pick up the bottle and hold it in your hand for 30 seconds and u don't have to set it down because of the heat pour that shit. If your holding it but u have to set it down after a few seconds because it's starting to burn too much. Guess what. It's a little to hot 🤷 Again, to each there own.
I'm trying to clean up these cultures with black tea agar. What do ya think. I don't think it's out run the bacteria yet
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Bph
Stranger



Registered: 10/11/18
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Re: Cultivation General Discussion [Re: Yesum]
#26412057 - 01/02/20 08:04 AM (4 years, 28 days ago) |
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If you pour your plates before you need them the condensation will clear up in a few days IME. So you just make a cup of tea and add agar or is there some nutes in there as well?
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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: Bph]
#26412070 - 01/02/20 08:19 AM (4 years, 28 days ago) |
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Quote:
Bph said: If you pour your plates before you need them the condensation will clear up in a few days IME. So you just make a cup of tea and add agar or is there some nutes in there as well?
There is a thread in in my signature of Franks stuff where he tests lots of different agar plates made from different recipes. Its a very interesting read.
https://www.shroomery.org/forums/showflat.php/Number/18676888#18676888
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
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Re: Cultivation General Discussion [Re: Edmunter] 1
#26412472 - 01/02/20 12:46 PM (4 years, 28 days ago) |
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BOD suggests use a cup with warm water and a flat bottom, put it on top of the dish for a few seconds-minutes (wont hurt if the water is warm-hot) and boom your dishes are crystal clear again.
haven't tried it myself but worth a shot
https://www.shroomery.org/forums/showflat.php/Number/21922023
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feldman114
Stragler


Registered: 09/06/19
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Re: Cultivation General Discussion [Re: tealseal]
#26412483 - 01/02/20 12:52 PM (4 years, 28 days ago) |
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For pasty plates, covering the GE hole with a paper towel, then foil completely eliminated condensation.
Just a little piece, like so.

I also found that I can reuse the foil “cups”, only replacing the paper towel piece for posterity.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
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Re: Cultivation General Discussion [Re: feldman114]
#26412533 - 01/02/20 01:30 PM (4 years, 28 days ago) |
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I had less than enthusiastic results with that. But that could be due to not having a cup with a flat bottom that would cover the whole plate.
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A.k.a
Stranger


Registered: 10/27/19
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It works great but they cloud up again. Gotta get that water out somehow.
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LAGM2020     
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: A.k.a]
#26412590 - 01/02/20 01:57 PM (4 years, 28 days ago) |
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Quote:
Randalf the Grey said: I had less than enthusiastic results with that. But that could be due to not having a cup with a flat bottom that would cover the whole plate.
It doesnt have to have a flat bottom I use small jars or media bottle with hot water or agar in them. They have the curved bottoms on them. They work for the tops but I always have condensation droplets on the sides of petris. Well in the winter anyways. During summer I dont remember condensation being a problem.
Also as far as the condensation coming back I think this means you aren't leaving it long enough I found that if I wait till the condensation is gone then take of the glass the condensation will come right back. Instead I leave the stacks with hot water on top until the hot water has came down to room temp for a little bit then I can take off the glass and wrap bag or use petris. I'm just getting back into using petris after a long break from them so I'm relearning most of this but I'm picking it back up super quick.
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