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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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I still make slight tweaks to my notations but for the most part it's variant abbreviation and date. For plates I include T1,T2,T3... E.G. AA+1230 for Albino A+ nocced on December 30th As I transfer them, I keep the original date name and add a T#
When I spawn a tub, I keep the date name and write the date of spawning tub on the lid. E.G. AA+1230 0114 if I spawned on January 14th.
This is why I like using painter tape for labeling. Just peel it off the jar and slap it on the tub.
For G2G, I write the date of noccing the jar and keep track of which jar it came from in notebook.
Check out my Book of Genesis link in my sig and you'll see more complex examples of how I used to track transfers.
Edited by Randalf the Grey (12/30/19 06:40 PM)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: Inocuole]
#26408334 - 12/30/19 07:17 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: It seems like thereβs a lot of bacteria problems with oats in particular.
Whatβs the benefit of using them assuming theyβre more prone to contamination?
Is it mainly for people doing large scale grows where once you master prep itβs significantly cheaper than wbs?
Just price and availability. They work for alot of people. Personally dont like oats. Still use them though lol.
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: gizmo1]
#26408352 - 12/30/19 07:26 PM (4 years, 1 month ago) |
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If theyre all you can get or super cheap use them, but IME they almost always look at least slightly bacterial even when the grain going into it is perfect. I did a run recently where i used the last of my oats mixed with my wheat and the straight wheat bags just look waaay nicer everything else was the same. Theyre also more voluminous by weight. The same sized bag full of wheat or rye has about 10-20% more grain by weight. Thats reason enough to switch if you can get anything else for a comparable price. Hell Iβd pay double just to see nicer looking spawn, even if its just for my own piece of mind and the bacterial looking oats are actually clean IDK fuck oats.
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π΄ π° πΌ π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 7 hours
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Quote:
gizmo1 said: Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon.
I think $78 is about the best price we'll find, especially for Celltreat.
Quote:
Colorblind-Myc said: How are ya'll documenting? Like serial numbers, some combination of abbreviations and dates? I've been refining my method all the time and nothing feels good enough.
I've really wanted to talk about this for a while, but it's been low priority. I think i will start a thread sometime (or if someone else wants to do it!), but i would LOVE to see what other people document. I feel like i'm going overboard, but then sometimes i feel like it's not enough.
Master list of plates, notes about times or technique, running costs, even from whom i received prints/swabs!
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26408366 - 12/30/19 07:37 PM (4 years, 1 month ago) |
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I hardly even label anything after master jars. And I have absolutely no paper (or digital) records of anything. I put stick notes on shelves For bags and jars and use dry erase board for tasks so I dont get sidetracked. Only thing I need to know is the next step, only save clones that are good and typically only run 2-3 varieties at a time and try to pick distinctive ones.
-------------------- Learn about breeding
  C10βs agar guide Good surface conditions = Good pinsets Read more, post less. π
π° πΌ π΄ π
π΄ π° πΌ π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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iEnter
Stranger


Registered: 03/17/16
Posts: 75
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: gizmo1]
#26408370 - 12/30/19 07:39 PM (4 years, 1 month ago) |
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Quote:
gizmo1 said: So it could be bacteria from the culture or that you introduced when inoculating it's not always from grains being too wet.
Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon. They are manufactured by celltreat so I know quality is good. I know I can also get cheaper but I would prefer to go with amazon if possible.
Maybe but I transferred a wedge of agar to each jar, and they are all having the same problems. I'm going to see how these next jars do
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Colorblind-Myc



Registered: 12/16/19
Posts: 144
Loc: Rockies
Last seen: 5 months, 29 days
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26408384 - 12/30/19 07:46 PM (4 years, 1 month ago) |
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Quote:
MLPismyOPSEC said:
Quote:
gizmo1 said: Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon.
I think $78 is about the best price we'll find, especially for Celltreat.
Quote:
Colorblind-Myc said: How are ya'll documenting? Like serial numbers, some combination of abbreviations and dates? I've been refining my method all the time and nothing feels good enough.
I've really wanted to talk about this for a while, but it's been low priority. I think i will start a thread sometime (or if someone else wants to do it!), but i would LOVE to see what other people document. I feel like i'm going overboard, but then sometimes i feel like it's not enough.
Master list of plates, notes about times or technique, running costs, even from whom i received prints/swabs!

Oh man, that pic speaks to the programmer in me haha. If you get a thread going, or maybe a journal? That'd be something I would totally follow.
Edited by Colorblind-Myc (12/30/19 07:48 PM)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: iEnter]
#26408412 - 12/30/19 08:13 PM (4 years, 1 month ago) |
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Quote:
iEnter said:
Quote:
gizmo1 said: So it could be bacteria from the culture or that you introduced when inoculating it's not always from grains being too wet.
Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon. They are manufactured by celltreat so I know quality is good. I know I can also get cheaper but I would prefer to go with amazon if possible.
Maybe but I transferred a wedge of agar to each jar, and they are all having the same problems. I'm going to see how these next jars do
Got a pic of plates used to inoculate?
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: gizmo1]
#26408576 - 12/30/19 10:52 PM (4 years, 30 days ago) |
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Oh totally, char the shit outta it, and my handle. Makes me feel safe and warm.
Wow, y'all keep track of shit. I jst sharpie the variety on the jar lid, plate, bin, or box. I dnt see dating things. Possibly cuz i cud only really concentrate on two varieties at a time. Not like i had a lab full of shit that all looked the same.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Cultivation General Discussion [Re: iEnter] 1
#26408590 - 12/30/19 11:02 PM (4 years, 30 days ago) |
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Quote:
iEnter said:
Quote:
gizmo1 said: So it could be bacteria from the culture or that you introduced when inoculating it's not always from grains being too wet.
Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon. They are manufactured by celltreat so I know quality is good. I know I can also get cheaper but I would prefer to go with amazon if possible.
Maybe but I transferred a wedge of agar to each jar, and they are all having the same problems. I'm going to see how these next jars do
Ditch the oats
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: cronicr] 1
#26408593 - 12/30/19 11:03 PM (4 years, 30 days ago) |
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I just switched to wheat recently and I couldn't be happier.
-------------------- Learn about breeding
  C10βs agar guide Good surface conditions = Good pinsets Read more, post less. π
π° πΌ π΄ π
π΄ π° πΌ π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: AyePlus]
#26408601 - 12/30/19 11:14 PM (4 years, 30 days ago) |
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Fuck oats

And treat yourself to the grip ring celltreats.
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Cultivation General Discussion [Re: Feasoghorm]
#26408613 - 12/30/19 11:24 PM (4 years, 30 days ago) |
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All my cultures are clean but jars look bacterial. I'm switching back to WBS. Oats is cool but wbs was more forgiving.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: Hobbit GDF]
#26408622 - 12/30/19 11:43 PM (4 years, 30 days ago) |
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Lol man oats got a bad rep.
Any reason not to use 35mm petri dishes? They are cheaper. They are smaller. We transfer before the petri is fully colonized anyways.
Only thing I can think of is edges scare me I always feel like edges are where the nasties hide out. Also probably wont be doing three transfers to each dish anymore so that's a downside.
Should I go for the 35x15s?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Cultivation General Discussion [Re: gizmo1]
#26408630 - 12/30/19 11:55 PM (4 years, 30 days ago) |
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Good for starting and great for tiger drops
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: cronicr] 1
#26408640 - 12/31/19 12:09 AM (4 years, 30 days ago) |
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I'm just going to wait and go with some 60mm dishes. This is a pretty good price for a case though last one available. You get 960 petris per case for $89. https://www.amazon.com/Celltreat-229638-Non-Treated-Sterile-Working/dp/B00BKAI2T0/ref=mp_s_a_1_1?keywords=229638&qid=1577775886&sr=8-1 I just think they are a little small for my needs I can be pretty slow getting around to doing transfers and if I ever have a tam outbreak I want a little wiggle room to try n outrun it.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 5 hours
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Re: Cultivation General Discussion [Re: mushboy]
#26408681 - 12/31/19 01:40 AM (4 years, 30 days ago) |
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Quote:
mushboy said: Still dunk? When did we start
dunking grains never made sense to me considering we spawn the grains to water filled substrates
Is was a thing back in the day that everyone used to swear by - Frank and others, I never read the debunking of it and wondered what the great minds thought now.
https://www.shroomery.org/forums/showflat.php/Number/17616982#17616982
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 5 hours
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Re: Cultivation General Discussion [Re: Edmunter] 1
#26408683 - 12/31/19 01:42 AM (4 years, 30 days ago) |
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I think ive found religion

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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Cultivation General Discussion [Re: Edmunter]
#26408686 - 12/31/19 01:54 AM (4 years, 30 days ago) |
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Quote:
Edmunter said:
Quote:
mushboy said: Still dunk? When did we start
dunking grains never made sense to me considering we spawn the grains to water filled substrates
Is was a thing back in the day that everyone used to swear by - Frank and others, I never read the debunking of it and wondered what the great minds thought now.
https://www.shroomery.org/forums/showflat.php/Number/17616982#17616982
There's not much to debunk it was documented enough and in most cases shows it can help speed colonization.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 5 hours
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Re: Cultivation General Discussion [Re: cronicr]
#26408695 - 12/31/19 02:10 AM (4 years, 30 days ago) |
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Quote:
cronicr said:
Quote:
Edmunter said:
Quote:
mushboy said: Still dunk? When did we start
dunking grains never made sense to me considering we spawn the grains to water filled substrates
Is was a thing back in the day that everyone used to swear by - Frank and others, I never read the debunking of it and wondered what the great minds thought now.
https://www.shroomery.org/forums/showflat.php/Number/17616982#17616982
There's not much to debunk it was documented enough and in most cases shows it can help speed colonization.
I suppose we are what the next gen call old school fam
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