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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
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Re: Cultivation General Discussion [Re: A.k.a]
#26407889 - 12/30/19 02:32 PM (4 years, 1 month ago) |
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I just do a quick dip and swish in a baby food jar of iso. If, when I flame the blade, it pops more than a few times then I'll give it a wiperoo and re-dip\flame. Point of interest on the topic though, I learned important lesson about placement of my jar of ISO. Once after removing blade from flame but while still on fire, a larger-than-average piece of agar melted and dripped off the blade while still burning. Missed my iso jar by a couple of inches. if it had gone in, there would have been a whoosh and I would have thrown a little over it quickly to extinguish it so it would not have been catastrophic. But definitely not something I want to deal with. Fire is a gift from the gods when you want it, but an absolute nightmare when you don't. Because of this I set up my work area with torch between iso and SAB. I know this is common sense, just thought I would share.
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A.k.a
Stranger



Registered: 10/27/19
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Yeah that could’ve been bad. I’m kind of paranoid about fumes and fire, I won’t even torch the scalpel until it’s basically dry when I dip it before I start.
We used to do all kinds of crazy stuff as kids and I witnessed a few accidents from vapors expanding and igniting from pretty unbelievable distances. I don’t think iso would do much, but I know some fumes just sort of whoosh and others will explode.
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LAGM2020     
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: A.k.a]
#26407938 - 12/30/19 03:00 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: What does a blade look like after a thousand transfers?? I’m imagining basically a charred lump on a scalpel handle.
Also do you guys wipe the blade between transfers? I just started doing that to get the agar off before torching it after a couple chunks of agar going off like tiny popcorn.
Took this pic 6 months ago. Blade on the left was used for 2 years before replacing it with the blade on the right. The blade on the right is still on my handle and I use it A LOT. If you've seen any of my pics you'd know I grow a lot of both edibles and actives.

If it bends and starts to fall off the handle I just bend it back. After a few times the blade basically gets tempered and stays in the shape needed to stay on the handle
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
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Re: Cultivation General Discussion [Re: A.k.a]
#26407945 - 12/30/19 03:02 PM (4 years, 1 month ago) |
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Yeah always kept them a good distance apart. Just didn't occur to me that agar may drip off the blade. Just had to flip positioning of them. Used to keep torch farthest away from s a b to keep my hair from going in the flame when I lean over the box. And for sure.... I grew up surrounded by hazmat and chemistry books and a shed full of those material in my back yard. Used to ride with my dad to "help" fight fires. In situations like this, not just fire but where I know what I did wrong II I do it wrong, my first thought is not "oh crap this could go bad", it's "oh crap I knew better". it's like people who go on a hike without taking any gear water or flashlight, and then end up getting stuck out there. I can only feel so sorry for them. Last thing I want is to be a statistics that makes my hobbies look bad.
Haha nate, yeah that looks about right. I should add to the blade convo that up until now I had been using Xacto blades. I think they may be a bit thicker and are held more tightly so never had an issue with them warping and\or falling off. I already had Xacto blades from other hobbies so just used what I had. However, now I'm think I may just start using scalpels for those hobbies as well.
Edited by Randalf the Grey (12/30/19 03:06 PM)
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
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Re: Cultivation General Discussion [Re: A.k.a]
#26407949 - 12/30/19 03:04 PM (4 years, 1 month ago) |
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I'm not getting why one would dip in iso b4 flaming. Red hot blade will be sterile, dipped or not.. Cleaning off pieces of agar b4 flaming I understand
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feldman114
Stragler


Registered: 09/06/19
Posts: 3,365
Loc: Bravos
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Re: Cultivation General Discussion [Re: Tweeq]
#26407952 - 12/30/19 03:05 PM (4 years, 1 month ago) |
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Quote:
Tweeq said: I'm not getting why one would dip in iso b4 flaming. Red hot blade will be sterile, dipped or not.. Cleaning off pieces of agar b4 flaming I understand
Yeah, there’s no like logical reason for the iso, is there?
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A.k.a
Stranger


Registered: 10/27/19
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Re: Cultivation General Discussion [Re: feldman114]
#26407959 - 12/30/19 03:08 PM (4 years, 1 month ago) |
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I meant the whole handle I don’t dip the blade.
That picture of the used blade is pretty crazy, next time I buy blades I’m gonna look for those kind with the open bottom, mine are connected at the bottom and makes it harder to get on and off.
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LAGM2020     
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
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Re: Cultivation General Discussion [Re: feldman114]
#26407965 - 12/30/19 03:12 PM (4 years, 1 month ago) |
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Quote:
feldman114 said:
Quote:
Tweeq said: I'm not getting why one would dip in iso b4 flaming. Red hot blade will be sterile, dipped or not.. Cleaning off pieces of agar b4 flaming I understand
Yeah, there’s no like logical reason for the iso, is there?
To remove small bits of agar\myc. I realize the flame with sterile it, the swish is to remove physical pieces that I just don't want in my plates and can be done more quickly than wiping blade each time. The more fluid I can be, the better. I use 90% iso for this application because it is more about a solvent than an antiseptic. (Again, other hobbies mean I always have 90% iso on hand as a solvent) I also keep scalpel\forcepts in iso while not being used. Not saying you should do it, just telling you what I do.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
Randalf the Grey said: That's actually the reason why I don't use no pours. I can't bring myself to throw out plates, on the off chance that I'll lose everything and this one ify looking plate might be my one chance to bring it back. With no pours, they took up so much damn room after only two sessions of transfers, I was running out of places to put them. at least with Petri dishes they stacked neatly and don't take up much room.
Dont be a plate packrat bro toss them old t1s. Once you get more confident in your abilities you wont need to keep them around. I get that nonpours can be annoying and bulky but I am doing what I can to reduce my waste while also using real petris. I became pretty proficient in no pours. They aren't the most pleasant to use but I will continue to use them as well as petris.
I think about it and a petri is around 15-20 cents a piece. Plus the cost of agar I wanna say around $3 for 50 plates I might be way off though. So another let's say 5-6 cents of agar so 25 cents per poured plate. It takes about 5-8 transfers until I see nice uniform growth like I Like. Then it goes to grain and I clone then do one extra transfer before going back to grain. So I'm using about 10 petris per variety if I only take one clone. That's about 2.50 in materials. Working with 10 different types of mushrooms at a time this can get expensive. With no pours I'm cutting out the cost of the petris and pc runs for the same amount of time. So let's say 6 cents per transfer as opposed to .25 cents. Or something like that so in cutting my costs down in less than half by doing a couple transfers in no pours. I'll start and finish on petris. So to me it makes sense financially and in order to cut back on waste. Idk but in my mind it's all justified. I sleep comfortably at night lmao. Also dont make fun of my math skill it was quick math plus I've never been good at math.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: gizmo1]
#26407980 - 12/30/19 03:19 PM (4 years, 1 month ago) |
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Yup the dip is to rinse off agar. I dont dip anymore because I end up with bent tips. What I do instead is keep a paper towel infront of sab I wipe both sides of the blade before flaming. Just a quick swipe across the paper towel and I'm good to go.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
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Re: Cultivation General Discussion [Re: gizmo1]
#26407994 - 12/30/19 03:26 PM (4 years, 1 month ago) |
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I can dig that Gizmo, for sure. I'm sure I'll give no pours another shot at some point, I just love my petris. They look so much cleaner. and honestly, the waste aspect of it is part of the reason I hang on to him as long as I do. Just feel bad throwing things away. But I do have tons of incredibly tiny metric screws and wires and terminals for various electronics that I have started reusing plates as holders for. You can fit a surprising amount of .4mm nozzles in a dish. so while it's still not a perfect solution,I try to minimize the amount of waste I'm creating.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
Randalf the Grey said: I can dig that Gizmo, for sure. I'm sure I'll give no pours another shot at some point, I just love my petris. They look so much cleaner. and honestly, the waste aspect of it is part of the reason I hang on to him as long as I do. Just feel bad throwing things away. But I do have tons of incredibly tiny metric screws and wires and terminals for various electronics that I have started reusing plates as holders for. You can fit a surprising amount of .4mm nozzles in a dish. so while it's still not a perfect solution,I try to minimize the amount of waste I'm creating.
I hear ya man I started saving all paper and cardboard scraps to make "fire bricks" and composting all my food scraps to reduce my waste some wish I never started though because now I feel guilty about waste. I bought reusable glass water bottles and stopped buying as many single use plastics as possible. I hate it lol. Also I love the way a nice clear petri looks with a nice uniform circle of mycelium in the middle makes me feel all sciencey lol.
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iEnter
Stranger


Registered: 03/17/16
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Quote:
Randalf the Grey said:
Quote:
iEnter said: Good looking my dudes!
Question though: To me without you pointing out that corner I would have thought thats myc. What are you seeing that made you decide it isn't? Is it the fact that it's "thicker" that's the only difference I see. I've never set foot in the contam forum, I might have to spend some time there.
That's it. Just the appearance of it. Thinker and fluffier than cube myc. I'm still pretty new myself and still remember my first grows. I remember having the same thought as you and discovering I was wrong. That's why it jumped out at me so much. After you see it enough times, you just kinda learn to recognize it.

It looks like we have another one! This is on an APE jar. I legit thought these were dry enough but apparently not. This next set of jars I'm doing I left the oats to strain in the fridge 2 days, so if that dosen't do the trick I'm not sure what will
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gizmo1



Registered: 06/15/11
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Loc: FREEDOM
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Re: Cultivation General Discussion [Re: iEnter]
#26408162 - 12/30/19 05:11 PM (4 years, 1 month ago) |
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So it could be bacteria from the culture or that you introduced when inoculating it's not always from grains being too wet.
Also does anyone know a good source for a case of 500 petris I found one for 78 on Amazon. They are manufactured by celltreat so I know quality is good. I know I can also get cheaper but I would prefer to go with amazon if possible.
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Randalf the Grey
Woodland Creature


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Re: Cultivation General Discussion [Re: gizmo1]
#26408173 - 12/30/19 05:16 PM (4 years, 1 month ago) |
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Those are the ones I go went with. But only because of recommendations on here. I haven't used any others.
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A.k.a
Stranger


Registered: 10/27/19
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It seems like there’s a lot of bacteria problems with oats in particular.
What’s the benefit of using them assuming they’re more prone to contamination?
Is it mainly for people doing large scale grows where once you master prep it’s significantly cheaper than wbs?
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LAGM2020     
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Lol did ya claw your way outta Shawshank with that blade? It's less than 10bux for 100 and a new handle. Really tho, i prolly go thru so many cuz i torch that shit till it's glowing like the sun.
Fuck oats!
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Randalf the Grey
Woodland Creature


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Re: Cultivation General Discussion [Re: A.k.a]
#26408190 - 12/30/19 05:31 PM (4 years, 1 month ago) |
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Having not used it, I do know from others here that it is significantly less expensive. Something like $5 for 50lb bag.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
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Re: Cultivation General Discussion [Re: Feasoghorm] 1
#26408208 - 12/30/19 05:43 PM (4 years, 1 month ago) |
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Quote:
GypsyCurse said: Lol did ya claw your way outta Shawshank with that blade? It's less than 10bux for 100 and a new handle. Really tho, i prolly go thru so many cuz i torch that shit till it's glowing like the sun.
Fuck oats!
You ruin the temper that way and the blade will die. Cutting agar and myc? Fuck, one blade should last forever if you just sterilize it in the flame.
I've used a single piece scalpel that I got forever ago from FP and once in a while I just sharpen it up if needed. But it cuts and spears and is easy to flame the surface of with a methanol fueled alcohol burner, which is all I use. WTF are contams going to do in a 3500 degree flame anyway except die immediately? 
OTOH if it makes you feel good torch it to hell.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Colorblind-Myc



Registered: 12/16/19
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Re: Cultivation General Discussion [Re: PrimalSoup]
#26408256 - 12/30/19 06:25 PM (4 years, 1 month ago) |
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How are ya'll documenting? Like serial numbers, some combination of abbreviations and dates? I've been refining my method all the time and nothing feels good enough.
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