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Randalf the Grey
Woodland Creature



Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: Cultivation General Discussion [Re: Jooby]
#26397939 - 12/23/19 05:07 PM (4 years, 1 month ago) |
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Quote:
Jooby said: So...I just throw it all out and start over?
I would not do that just yet. They do look bacterial, but this is why I love agar. Cleaning up some MSS on agar myself right now. You just need more time, more plates, and more agar.
Cut 3 or 4 small wedges out of the leading edge of growth in each plate and put on new plates. Then (or if you want to wait, do this first) put those first plates in ziplock bags and put in the fridge for a few days. This will help the myco outrun the contam. Then repeat transfers at leading edge. Rinse and repeat until you have clean myco.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cultivation General Discussion [Re: Jooby]
#26397942 - 12/23/19 05:09 PM (4 years, 1 month ago) |
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Quote:
Jooby said:
Quote:
Edmunter said:
Quote:
Jooby said: Could I get some opinions/advice please. I have 2 sets of 7 MS>G jars. First jars were done stupidly on 12/7 without holes in the lids for GE. They are officially stalled now. Should I bother pc'ing some lids to swap in my SAB? Here is 1 jar
 These jars, noc'd on 12/14, I thought looked good but not sure now.

Some advise on transferring these plates would be great also. 3 of the 4 look good to me but idk.

Please & Thank you!!!!
Sorry to say but all those plates need work. Take a fresh spore print inoculation to be safe so you ca start again as id say they all have contamination
The greasy stuff is a bacteria you can get a flush from but it will fuk you during or st8 after it fruits
The jars are fucked.... Dont give up buddy
Spore prints sound great but if I had them I wouldnt be messing with syringes in the first place. This is all new to me. First attempt at it. So...I just throw it all out and start over?
Where are u from man? Im Europe based and can help if u are this way? That syringe is contaminated 100% Tell your vendor
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: Edmunter]
#26397947 - 12/23/19 05:10 PM (4 years, 1 month ago) |
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I dont really think of it as poor practice or recommend against trying them out. I just know they held alot of allure for me when I first started and the potential for rapid expansion of mycelium in a liquid form is super cool, I just realized I dont like it as much as other ways of expansion because of the drawbacks that come with it.
Buuut all that aside, IIIIF (big if) I was trying to fill a warehouse with grain spawn I’d consider it. Would probably make full quarts with the josex poke method and Make a couple extranof each variety just in case, then Test the LC using a sterilized swab or a flamed loop to streak it on agar about 3-5 days before cooking grain and pour 10-20cc’s per bag or use a veterinary style auto injection gun(like verums). In that situation I can’t think of a better way than LC. But as long as I’m only cooking a few bags at a time I can easily have enough jars ready to g2 them
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Jooby
Stranger



Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Edmunter]
#26397952 - 12/23/19 05:12 PM (4 years, 1 month ago) |
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That sucks so bad. I got burnt for my first order, now this is my 2nd order. Aaaaahhhhhh.
I'm in USA...thank you so much though, I really appreciate the offer.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cultivation General Discussion [Re: Yesum]
#26397953 - 12/23/19 05:12 PM (4 years, 1 month ago) |
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Quote:
Yesum said:
or  
Id go 3 plates from this with the aim to make 3 masters

There is plenty of good genetics in that trash bag lol
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Randalf the Grey
Woodland Creature



Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: Cultivation General Discussion [Re: Jooby]
#26397962 - 12/23/19 05:16 PM (4 years, 1 month ago) |
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Quote:
Jooby said: That sucks so bad. I got burnt for my first order, now this is my 2nd order. Aaaaahhhhhh.
I'm in USA...thank you so much though, I really appreciate the offer.
If you check out the sponsor forum, one of our (reliable IMO) sponsors is having a holiday sale right now. I just ordered some this morning.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cultivation General Discussion [Re: AyePlus]
#26397963 - 12/23/19 05:16 PM (4 years, 1 month ago) |
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Quote:
AyePlus said: I dont really think of it as poor practice or recommend against trying them out. I just know they held alot of allure for me when I first started and the potential for rapid expansion of mycelium in a liquid form is super cool, I just realized I dont like it as much as other ways of expansion because of the drawbacks that come with it.
Buuut all that aside, IIIIF (big if) I was trying to fill a warehouse with grain spawn I’d consider it. Would probably make full quarts with the josex poke method and Make a couple extranof each variety just in case, then Test the LC using a sterilized swab or a flamed loop to streak it on agar about 3-5 days before cooking grain and pour 10-20cc’s per bag or use a veterinary style auto injection gun(like verums). In that situation I can’t think of a better way than LC. But as long as I’m only cooking a few bags at a time I can easily have enough jars ready to g2 them
No way, make master jars and go from there. LC is a longwinded crap shot, for fun, noting comercial unless you are using fast growing myc like oysters
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Jooby
Stranger



Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Edmunter]
#26397978 - 12/23/19 05:25 PM (4 years, 1 month ago) |
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I tried to quote each of you who responded but I'm retarded rn. Thank you guys!
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cultivation General Discussion [Re: Jooby]
#26397995 - 12/23/19 05:34 PM (4 years, 1 month ago) |
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Quote:
Jooby said: I tried to quote each of you who responded but I'm retarded rn. Thank you guys!
Just press quote and then write your stuff.
A dirty syringe is so hard to work with as a beginer. Try inoculating a plate and then the first growth you see transfer st8 away. If u can use agar all you want is 1 mushroom you can clone, then from that you can take 5 plates and get decent mycellium. I know it sound hard but clones material grows super fast and is easy to police...whoop whoop...thats the sound........
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Edmunter]
#26398012 - 12/23/19 05:45 PM (4 years, 1 month ago) |
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ya, thats the easiest way.
also @ crack, can u just go hunting instead? totally not cult. lol!
also @ ayeplus: yeah, if you make 20 master jars ad g2(x10) to 200 bgs why need lc? and thats leaving out g2 jar which would give u 2,000. just sayin'. I like it mainly to give others ease of use upon receiving in trade and to know I can make it. dont use it on the reg tho
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
Edited by TedsDead (12/23/19 05:51 PM)
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station
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Re: Cultivation General Discussion [Re: TedsDead] 1
#26398018 - 12/23/19 05:49 PM (4 years, 1 month ago) |
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Check her out.
APE

There was 3 others that weigh 14dry (total)
Edited by Hobbit GDF (12/23/19 05:50 PM)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Cultivation General Discussion [Re: Hobbit GDF]
#26398035 - 12/23/19 05:57 PM (4 years, 1 month ago) |
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How long you guys let the air settle before sab work?
I just moved my station to a new room, it makes me kind of nervous. its my weed smoking shed/building in the back yard. I go in and out of the room like 10-20 times a day
-------------------- Tek's I use LAGM2020
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Jooby
Stranger



Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Edmunter]
#26398043 - 12/23/19 06:02 PM (4 years, 1 month ago) |
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Quote:
Jooby said: Could I get some opinions/advice please. I have 2 sets of 7 MS>G jars. First jars were done stupidly on 12/7 without holes in the lids for GE. They are officially stalled now. Should I bother pc'ing some lids to swap in my SAB? Here is 1 jar
 These jars, noc'd on 12/14, I thought looked good but not sure now.

Some advise on transferring these plates would be great also. 3 of the 4 look good to me but idk.

Please & Thank you!!!!
Sorry to say but all those plates need work. Take a fresh spore print inoculation to be safe so you ca start again as id say they all Quote:
Edmunter said:
Quote:
Jooby said: I tried to quote each of you who responded but I'm retarded rn. Thank you guys!
Just press quote and then write your stuff.
A dirty syringe is so hard to work with as a beginer. Try inoculating a plate and then the first growth you see transfer st8 away. If u can use agar all you want is 1 mushroom you can clone, then from that you can take 5 plates and get decent mycellium. I know it sound hard but clones material grows super fast and is easy to police...whoop whoop...thats the sound........
My intentions in the first place were to do multiple transfers from the first plates, to clean them up. I really dont care much about the jars, as that was an impulsive afterthought. I intend to cut the leading edges from the 3 plates I think look ok. The fridge advice will be used too! Th
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TedsDead



Registered: 01/03/17
Posts: 4,998
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well, weed smoke is no good for the sab. may drape down contams. can u grip and rip elsewhere? Ive had good luck in sheds just mist that shit hrd with h2o and go once the droplets settle
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Cultivation General Discussion [Re: TedsDead]
#26398057 - 12/23/19 06:09 PM (4 years, 1 month ago) |
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I usually dont smoke in the room, sometimes the occasional dab but most of the time I smoke outside or with the door open.
Im more concerned about the outside air, I have to walk outside to get to the room.
-------------------- Tek's I use LAGM2020
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Quote:
staytrippy420 said: How long you guys let the air settle before sab work?
I just moved my station to a new room, it makes me kind of nervous. its my weed smoking shed/building in the back yard. I go in and out of the room like 10-20 times a day 
a million Ping pong balls made out of feather bouncing around your front room . If you sprayed them all with water how many ping pong balls would be left bouncing around your air flow. Then if you walked from another room full of pingpong balls that wasnt sprayed with water how many ping pong balls would u bring in with you?
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: TedsDead]
#26398069 - 12/23/19 06:16 PM (4 years, 1 month ago) |
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I mean yeah that works for the first 200 bags but if you’re trying to do that weekly thats a lotta work compared to making enough LC to noc the same, 7 quart jars of LC could do 700 bags and thats only one pc run and sterile sesh. With grain you max out around 70 from same or larger amount of effort.
I gues smy point is at a certain scale it makes perfect sense. But for home cult its overkill.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Cultivation General Discussion [Re: Edmunter]
#26398074 - 12/23/19 06:17 PM (4 years, 1 month ago) |
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Quote:
Edmunter said:
Quote:
staytrippy420 said: How long you guys let the air settle before sab work?
I just moved my station to a new room, it makes me kind of nervous. its my weed smoking shed/building in the back yard. I go in and out of the room like 10-20 times a day 
a million Ping pong balls made out of feather bouncing around your front room . If you sprayed them all with water how many ping pong balls would be left bouncing around your air flow. Then if you walked from another room full of pingpong balls that wasnt sprayed with water how many ping pong balls would u bring in with you?
I was never good at math.
-------------------- Tek's I use LAGM2020
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
Randalf the Grey said:
Quote:
gizmo1 said:
Quote:
Randalf the Grey said: Excellent. Now I just need to find some RTV silicone in the next couple hours and I'm good to go. I had to lay in the floor earlier to prep my grain jars, but I'm not going to let this nerve pain stop me from getting shit done on the time off that I have. can't reach high enough to brush my hair or stand up straight, so definitely about to get some weird looks at the store though.
That sucks man I've got a leg injury and am stuck in bed for the next couple days that's why I've been posting alot so you guys are all stuck with me for a little longer then I'll go back to being the recluse I've always been. Aka back to busy life lol. I figure after Christmas I should be able to walk again.
Samesies man. Haha, I almost feel bad cause I know I have been blowing up these threads but yeah, I can't move or go anywhere so Ive just been sitting here, reading and planning.
Same man definitely not having a good time but I glad the shroomery is here for me in my time of need lol.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Cultivation General Discussion [Re: AyePlus]
#26398095 - 12/23/19 06:31 PM (4 years, 1 month ago) |
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Quote:
AyePlus said: I mean yeah that works for the first 200 bags but if you’re trying to do that weekly thats a lotta work compared to making enough LC to noc the same, 7 quart jars of LC could do 700 bags and thats only one pc run and sterile sesh. With grain you max out around 70 from same or larger amount of effort.
200 fuked bags u mean? id rather grow 1000 agar cultures if i was pro level. Maybe im wrong but id get a rotation going. say u use 200 cultures, a month to colonise 1 bag. 4 months in your 600 bags good and fruiting to rotation and can upscale at anytime?
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