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Offlinebongoman
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Registered: 09/19/18
Posts: 558
Last seen: 7 days, 1 hour
Re: Cultivation General Discussion [Re: Failboat]
    #26367973 - 12/07/19 10:13 PM (4 years, 1 month ago)

All of a sudden my agar to agar transfers in a SAB are seeing massive rates of contamination. I’m talking 90% mold satellites across my no pour jars, suggesting airborne contaminants.

As far as I can tell, here’s what I've been doing differently in these problematic SAB sessions:

  • a new, larger (120 litre SAB)
  • hosing SAB out using garden hose outside prior to session and not sanitising interior with alcohol
  • maybe not leaving for long enough to allow air to settle before session
  • not bringing all donor and receiver plates into the SAB prior to the session, but bringing them in in “shifts” and then wiping down with an iso paper towel and not spraying inside the box again
  • not spraying interior of box during the session
  • not showering or changing into clean clothes prior to session


Otherwise I’m wiping my gloves off with iso, not hovering my hand over exposed agar, no obvious air currents in room, and am adequately flame sterilising my scalpel.

It’s been a real blow to hit this road block - could any of the above factors alone or in combination, explain my sudden and dramatic contamination rates?



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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: bongoman]
    #26367978 - 12/07/19 10:18 PM (4 years, 1 month ago)

I found most satellites come from my scalpel when trying to place me wedge.
Or if your trying to transfer from plates with mold colonies your myc likely has spores on it as well


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 50 minutes
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Re: Cultivation General Discussion [Re: bongoman]
    #26367981 - 12/07/19 10:19 PM (4 years, 1 month ago)

Sounds like the bringing things in in shifts coupled with not waiting long enough. Is your HVAC blowing? I like to turn mine off.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Failboat]
    #26368070 - 12/07/19 11:15 PM (4 years, 1 month ago)

I was having SAB issues like that with most my shit contaminating. Turned out it was the breath out my nose hitting my arms and rollin' on into the SAB.


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26368098 - 12/07/19 11:42 PM (4 years, 1 month ago)

Quote:

GypsyCurse said:
I was having SAB issues like that with most my shit contaminating. Turned out it was the breath out my nose hitting my arms and rollin' on into the SAB.



That elicited a very serene image in my head 😂 a sporey breath cloud rolling into the sab


--------------------
If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: jbgtaa] * 1
    #26368105 - 12/07/19 11:48 PM (4 years, 1 month ago)

Haha, that's exactly what was happening. In fact, i snot-rocketed on a plate to see what i was dealing with:


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Offlinebongoman
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26368145 - 12/08/19 12:31 AM (4 years, 1 month ago)

Quote:

GypsyCurse said:
I was having SAB issues like that with most my shit contaminating. Turned out it was the breath out my nose hitting my arms and rollin' on into the SAB.



Do you wear a mask when doing SAB work? How did you deal with the issue?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: bongoman]
    #26368179 - 12/08/19 01:03 AM (4 years, 1 month ago)

The bottom of my SAB was textured so i cudnt see through it. I was working in front of the arm holes where i cud see. That was the main issue.
I cut a hole in the bottom (upside down ends up being the top) and stretched saran wrap over it. I wore a handkerchief and started looking through the top with my body close to the sab. Arms all up in it. Cut my contam rate to nearly nothing almost immediately. I remember that first run of clean plates, i bout nutted.
I also spray the whole inside with iso (works for me but some will say is not necessary), wear gloves, and constantly wipe my hands and arms with lysol wipes.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Edmunter]
    #26368185 - 12/08/19 01:13 AM (4 years, 1 month ago)

Quote:

Edmunter said:
The first clone isnt great but the other 2 (same clone as each other)  are keepers for now.







These side pin like crazy so if they fruited on top it would be a banger




Definitely a keeper cluster clone, look how quick these bad boys developed.



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OfflineSmoothcat
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Registered: 10/07/15
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Re: Cultivation General Discussion [Re: Failboat]
    #26368222 - 12/08/19 02:04 AM (4 years, 1 month ago)

Quote:

Quirkmeister92 said:
Quote:

Smoothcat said:
The tribe is awesome... meaculpaUIO is definitely Willie myco

:watchingyou:



:canthelpbutlaugh:




        :awedrugs:


--------------------
Back once again with the ill behaviour



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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: Cultivation General Discussion [Re: Smoothcat] * 2
    #26368310 - 12/08/19 05:00 AM (4 years, 1 month ago)

Here we have all we need to know about everything.

the tribe is awesome and Meaculpo is willy myco, and the tribe see's willy from behind. nice.

The tribe is fucking awesome. SunnyDaze summed it up about perfectly. I think jordan peterson has a shpeel on relationships that sums it up rather nicely too. The basic jist is this.

Humans have a deep desire to know and be known but learn from a very early age (and rightly so) that shit gets proper fucked if you go around telling everybody everything. When you check out at the grocery store and the lady says, "how are you today", there is a reason you don't say,

"Well, I'm alright but I ate a salad with spicy ranch and jalapenos on it and it really kicked my gut in the nads. So i woke up this morning just about to sauce my bed and it took all I had to make it to the bathroom. I actually shit Myself a little and proceeded to blow flames for a straight half hour. The only interruptions were mandatory and quite painful baby wipe sessions. 

The whole time, even through the utter anguish of my borderline spontaneous combustion, what was really bothering me was the meaning of my dream. In it i was sitting in a church with an erection. Next to me was my mom (holding a taser), a priest, the first girl i ever kissed (whose name is sara thomas), a goat, a frog and jeffery epstein. The priest was reading from the Torah. Every time the priest finished reading a new "Thou shalt not" he'd lower the torah and look me in the eye. Then he lick his lips in a very disturbing way and it would send a tingling sensation through my body that would cause my muscles to tighten and my eyes to roll back. Upon seeing this happen, my mother would grab the taser and let her fly right into my erect penis. When this happened sarah thomas would start crying and confessing all of her daddy issues to Epstien. Craziest thing is, i kind of liked the penis tazing and found jeffery's jawline rather attractive.

It's actually turning me on just talking about it. What's your relationship with your dad like? How was your childhood? Any ways, Do you know the best way for me to clean ten thousand dollars. I've been making money in a way that isn't considered to be strictly legal and..."

No, to her you say, "I'm alright. How are you". You pay for and quickly consume some pepto and you go on with your day. Every person you ever interact with gets a different level of truth from you. All the way down to your closest friends, who get to hear the details of your sick and twisted dream and bowel movements.

The tribe is just a group of folks that get a little more honest with each other. It's a beautiful thing really.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: verum subsequentis] * 1
    #26368559 - 12/08/19 08:43 AM (4 years, 1 month ago)

:out:


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26368566 - 12/08/19 08:45 AM (4 years, 1 month ago)

:leocheers:


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OfflineSmoothcat
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26368650 - 12/08/19 09:31 AM (4 years, 1 month ago)

Live your dreams brothers and sisters

:raisemyglass:


--------------------
Back once again with the ill behaviour



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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26368680 - 12/08/19 09:44 AM (4 years, 1 month ago)

Let's not mislead: tribe is actually a secret organization of mercenary elves infiltrating the cultivation community in order to poison the planet with a false sense of awakening

:hyperlol:

Tribe til I die...

Alpha omega psi


Edited by Caps McGee (12/08/19 10:15 AM)


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OfflineRandalf the Grey
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Registered: 06/11/19
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26368928 - 12/08/19 12:02 PM (4 years, 1 month ago)

A quick question for users of SFD.
Figured cult discussion thread would be better that a new thread.

When lidding jars using SFDs do you order them:
jar, SFD, lid, ring
-or-
Jar, lid, SFD, ring
?
I have seen pics of both way so more looking for personal experience\anecdotes of which way and why.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26368931 - 12/08/19 12:03 PM (4 years, 1 month ago)

Let's get back to our regular scheduled program


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineFailboat
Fuck Up

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Posts: 8,736
Last seen: 8 hours, 50 minutes
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Re: Cultivation General Discussion [Re: Randalf the Grey]
    #26368937 - 12/08/19 12:06 PM (4 years, 1 month ago)

I use plastic lids modified with a hole and the filer over the hole. I reccomend the same as metal lids blow. Are you not cutting up your SFD? I would silicont metal lids inti 1 piece, drill a hole and affix your sfd piece. One disc can filter like 2 dozen jars if cut right.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26368938 - 12/08/19 12:07 PM (4 years, 1 month ago)

Are you a tribe member?


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OfflineRandalf the Grey
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26368947 - 12/08/19 12:12 PM (4 years, 1 month ago)

Meh, if I'm gonna go through the trouble of cutting and silicone-ing filters, I'll just keep using micropore tape. Got the discs purely for streamlining process. They are fairly inexpensive and I don't have more than 2 dozen jars at once max. I also run bags from time to time so no need to stretch them out at this point.

And no, I am not a tribe member. Do I need to be for this thread? If so, my bad , I did not realize.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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