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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: Samskara92]
#26359094 - 12/03/19 01:19 PM (4 years, 1 month ago) |
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Does anyone keep cloning clones till they get that bomb of a culture? I have some cluster culture that are close to what I want but think a few more transfers might get me there.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
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Re: Cultivation General Discussion [Re: Edmunter]
#26359120 - 12/03/19 01:38 PM (4 years, 1 month ago) |
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I thought the idea was to fruit ASAP and then take clones from some shrooms. Then you have a strain. How it turns out is the next step.
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cronicr



Registered: 08/07/11
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Re: Cultivation General Discussion [Re: Edmunter]
#26359125 - 12/03/19 01:40 PM (4 years, 1 month ago) |
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Quote:
Edmunter said: Does anyone keep cloning clones till they get that bomb of a culture? I have some cluster culture that are close to what I want but think a few more transfers might get me there.
Huh? Like clone a clone then clone? Why not just keep your original plate it's the same genetics
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



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Re: Cultivation General Discussion [Re: Inthepit]
#26359130 - 12/03/19 01:41 PM (4 years, 1 month ago) |
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Quote:
Inthepit said: I thought the idea was to fruit ASAP and then take clones from some shrooms. Then you have a strain. How it turns out is the next step.
Yep, but im talking 3 steps down the line when youve tested 3 clones and you like the mushrooms but not the quantity. Are the genetics for a mushroom bomb still in there?
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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: cronicr]
#26359135 - 12/03/19 01:43 PM (4 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
Edmunter said: Does anyone keep cloning clones till they get that bomb of a culture? I have some cluster culture that are close to what I want but think a few more transfers might get me there.
Huh? Like clone a clone then clone? Why not just keep your original plate it's the same genetics
For example, im pulling maybe a oz per shoe box first flush but I know it could be better. Can you still find better genetics in there?
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cronicr



Registered: 08/07/11
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Re: Cultivation General Discussion [Re: Edmunter]
#26359147 - 12/03/19 01:47 PM (4 years, 1 month ago) |
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I would go back to spores and try again, some clones aren't keepers
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: cronicr]
#26359154 - 12/03/19 01:49 PM (4 years, 1 month ago) |
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Quote:
cronicr said: I would go back to spores and try again, some clones aren't keepers
How many clones do you take from a ms grow to find the bomb? What do you look for?
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Inthepit
Aum Mani Padme Hum



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Re: Cultivation General Discussion [Re: Inthepit]
#26359164 - 12/03/19 01:53 PM (4 years, 1 month ago) |
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Ok, but there was a thread cautioning against going past G3. I think that's the term, meaning generation 1,2, and 3. After that yer going downhill. I would rather keep getting a biopsy from a MS fruiting. And hoping for the winner, winner, chicken dinner!
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LtLurker
Lost Sailor



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Re: Cultivation General Discussion [Re: Inthepit] 1
#26359169 - 12/03/19 01:57 PM (4 years, 1 month ago) |
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Quote:
Inthepit said: I thought the idea was to fruit ASAP and then take clones from some shrooms. Then you have a strain. How it turns out is the next step.
It's just a clone, not a strain. Strain is a single genetic code(2 spores mated). A clone still has a ton of strains in it and will sector on a plate.
That's not a generation. A generation runs from spore to fruit, next gen starting at spores. There's lots of posts about senesence, but many will say those concerns are over hyped and a g2g series is more likely to contam out before actually dying of old age. Slanting makes doing a huge number of plate transfers kinda moot when you can preserve an early sample.
After transferring from different sectors of a clone and narrowing those down more separately, I've notice some small differences in the typical result, but they were all still in the same ballpark. I wouldn't expect a killer producer hiding in a clone showing you so-so results.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Cultivation General Discussion [Re: meaculpaUIO]
#26359178 - 12/03/19 02:02 PM (4 years, 1 month ago) |
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Quote:
GypsyCurse said: Yeah i hear a lot of dudes dnt do shit and are fine. Jst not the case with some of us. Gotta pull out all the stops. Psh...fuck pulling out.
Point was, (IMHO) all those extra measures are crutches for (un)sterile technique... 
Quote:
meaculpaUIO said:
Quote:
feldman114 said: Oh but it’s ok to bitch and complain about another user in this thread?
At least his “discussion” was centered on “cultivation”, you know, in “general”...
its okay, the no flaming in cultivation doesnt apply when I'm the target
Omfgregg... need some cheese for our whine boys?(or girls, I'll not assume) the name of this site is "shroomery.org" NOT "dramamama.comma" (that's pretty good)
Anyone else ever noticed heavier pinset/stronger first flushes with bacterial spawn? I think I had bacteria riding a culture and have escaped from it: It's sped up a little, but dropped in yield dramatically: was averaging 6.5oz per gallon, now only giving 3.75-4.25/gal
Wtf Ober?
When picking clones, I suggest taking 3-5 from an ms tub to save time, ran side by side for easy comparison in speed and performance... I wouldn't mess with fruiting a clone culture more than once if it's no good: can't pull gold from a terd: if the genetics just aren't there, you'll NEVER find them there and are wasting time and resources on hope... back to spore if none are good candidates... right now, id focus on reliably producing clean ms spawn... once you have semi predictable results with ms, focus on isolation of characteristics
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Inthepit
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Re: Cultivation General Discussion [Re: LtLurker]
#26359187 - 12/03/19 02:07 PM (4 years, 1 month ago) |
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Thankyou for the clarification! Sorry but I have to ask. I thought a biopsy from a stalk would yield a single strain on the plate. That one shroom represents two spores.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Cultivation General Discussion [Re: Inthepit]
#26359208 - 12/03/19 02:17 PM (4 years, 1 month ago) |
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No... a clone will contain hundreds likely thousands of individual strains performing as one monoculture to produce a single fruit body: it's how ms works, and where genetic variation is carried between spore production from generation to generation... it truly is a LOT of work to get a single strain... I've got clone cultures on their 23rd transfers and STILL unpacking sectors
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LtLurker
Lost Sailor



Registered: 01/03/18
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Re: Cultivation General Discussion [Re: Inthepit]
#26359212 - 12/03/19 02:19 PM (4 years, 1 month ago) |
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no. one mushroom has many many strains working together to build the fruit. it's just more limited than all the strains present in a tub, plus you can see what they grew.
mushrooms do these cool things called clamp connections/anastosmosis where the strains will connect and share info, nutes, and work together like a single organism. They also sector, which is kinda inverse where they branch out as they overtake more area. Mushrooms are funky man.
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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: Caps McGee]
#26359229 - 12/03/19 02:26 PM (4 years, 1 month ago) |
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Quote:
Caps McGee said:
Quote:
GypsyCurse said: Yeah i hear a lot of dudes dnt do shit and are fine. Jst not the case with some of us. Gotta pull out all the stops. Psh...fuck pulling out.
Point was, (IMHO) all those extra measures are crutches for (un)sterile technique... 
Quote:
meaculpaUIO said:
Quote:
feldman114 said: Oh but it’s ok to bitch and complain about another user in this thread?
At least his “discussion” was centered on “cultivation”, you know, in “general”...
its okay, the no flaming in cultivation doesnt apply when I'm the target
Omfgregg... need some cheese for our whine boys?(or girls, I'll not assume) the name of this site is "shroomery.org" NOT "dramamama.comma" (that's pretty good)
Anyone else ever noticed heavier pinset/stronger first flushes with bacterial spawn? I think I had bacteria riding a culture and have escaped from it: It's sped up a little, but dropped in yield dramatically: was averaging 6.5oz per gallon, now only giving 3.75-4.25/gal
Wtf Ober?
When picking clones, I suggest taking 3-5 from an ms tub to save time, ran side by side for easy comparison in speed and performance... I wouldn't mess with fruiting a clone culture more than once if it's no good: can't pull gold from a terd: if the genetics just aren't there, you'll NEVER find them there and are wasting time and resources on hope... back to spore if none are good candidates... right now, id focus on reliably producing clean ms spawn... once you have semi predictable results with ms, focus on isolation of characteristics

I was just curious. I have a clone that is a cluster with 80% canopy so ok for now and quick but you know we always want the massive fruiting 2 weeks turn around clone. Working with 4/5 different clones at in the tubs but nothing has gone bang. Im hoping the RW MS grow will grab me a boomerclot clone to end all clones.........
im using shoeboxes and not sure as to how many flushes I should aim for. I need the space but dont want to just chuck stuff out.
What would we do without mushrooms? As I grow they become more important.
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Caps McGee
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Registered: 10/28/17
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Re: Cultivation General Discussion [Re: Edmunter]
#26359233 - 12/03/19 02:28 PM (4 years, 1 month ago) |
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My largest fruit have been PE6... Fucking stout too... overall yield still comparable
Amen to funky fungi! Thank Gregg too!
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Edmunter
Mr



Registered: 05/01/13
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Re: Cultivation General Discussion [Re: Caps McGee]
#26359244 - 12/03/19 02:31 PM (4 years, 1 month ago) |
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Quote:
Caps McGee said: My largest fruit have been PE6... Fucking stout too... overall yield still comparable
Amen to funky fungi! Thank Gregg too!
and how many flushes do you aim for?
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Inthepit
Aum Mani Padme Hum



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Re: Cultivation General Discussion [Re: LtLurker]
#26359247 - 12/03/19 02:31 PM (4 years, 1 month ago) |
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"Mushrooms are funky man. " Ya it's really captivating. I just had a major failure, Mushboy called it a biohazard! Oh Mommy! Anyway I'm already Jonesing to do some plate work. This time I'll do a few iterations before going to Oats.
Edited by Inthepit (12/03/19 02:35 PM)
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Edmunter]
#26359256 - 12/03/19 02:35 PM (4 years, 1 month ago) |
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Caps - No, you are wrong. It is all part of the sterile technique.
Edmunter - It feels like if you keep transferring your actually continually narrowing down genetics every time you transfer. I thnk Cron hit it well. Prolly not a good use of time searching for a bomb clone culture in a clone that hasent shown it yet.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: Inthepit]
#26359274 - 12/03/19 02:42 PM (4 years, 1 month ago) |
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Anymore, only 2... I find even these are hit and miss for predictable results... maybe with better maintenance during first flush, but ain't got time for that... I'm playing with less and less spawn and sub to find the sweet spot and minimize resource input for optimal utilization of surface area for 'one and done' SOS's in constant rotation... been recycling the substrates for gourmets (MUAHAHAHAHA) so not really concerned as far as being "wasteful"... blues and shiitake both LOVE the mixture of once fruited cube sub and once fruited sawdust blocks: colonizing and fruiting in some cases, in HALF the time...
YOUR technique, but absolutely NOT necessary to maintain sterility...
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Caps McGee]
#26359317 - 12/03/19 02:59 PM (4 years, 1 month ago) |
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Poke a buncha holes in your FH and tell me it's not necessary to patch them up. If 10% of your shit comes out sterile then it's possible and technically not necessary to patch all the little holes. One cud say that you must have crappy sterile technique cuz i have a 3% contam rate with my FH full of holes sort of thing.
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