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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Edmunter]
    #26327994 - 11/18/19 01:19 AM (4 years, 2 months ago)

You mean clone from the shitty clone ? I wouldnt fuck with it . You should be working with at least 15 different clones so you would have plenty to fuck with ..

5 tubs 3 clones per tub .. or go ham like me and do 40646790653278 varieties and 24589754214568976432567 clones Haha no really dont do that its pretty overwhelming


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OfflineFerather
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Re: Cultivation General Discussion [Re: cronicr] * 1
    #26328126 - 11/18/19 04:57 AM (4 years, 2 months ago)

Quote:

cronicr said:
I don't speak mycologenese either:lol:




Maybe this will help. Extract from my journal + more.

====


Active mycelium:


Baeocystin:  [C11]--[H15]--[N2]--[O4]--[P]
Norbaeocystin: [C10]--[H13]--[N2]--[O4]--[P]

Psilocybin:  [C12]--[H17]--[N2]--[O4]--[P]
Psilocin:  [C12]--[H16]--[N2]--[O]


From above:

"Psilocybin is a tryptamine compound with a chemical structure containing an indole ring linked to an ethylamine substituent. It is chemically related to the amino acid tryptophan, and is structurally similar to serotonin."
"Psilocybin is a member of the tryptophan-based compounds that originally functioned as antioxidants in earlier life forms before assuming more complex functions in multicellular organisms."

"Psilocin is relatively unstable in solution due to its phenolic hydroxy (-OH) group. In the presence of oxygen it readily forms bluish and dark black degradation products"
"Most species of psilocybin-containing mushrooms bruise blue when handled or damaged due to the oxidization of phenolic compounds"

"Psilocin is broken down by the enzyme monoamine oxidase. Some psilocin is not broken down, and forms a glucuronide"


Notes:

Nor-baeocystin are analogs of psilocybin, meaning single or various elements are added or removed.
It appears they can be phosphorylated or dephosphorylated (cleaved, added, a cycle?).
All four compounds contain carbon, hydrogen, nitrogen and oxygen.

Serotonin:  [C10]--[H12]--[N2]--[O]

====

I also Googled and found that "oxidative oligomerization", is a reaction catalyzed by oxidoreductases.
Other examples of oxidoreductases are laccase and peroxidases (target: phenols).

Lignicolous fungi commonly use laccase and-or peroxidases.

----

White rot fungi - enzymes and decay

https://imgur.com/a/8jngxe8

----

Quote:

Ferather said:
[Advanced], for informative purposes.

Here I germinated Cubensis spores on-in a wooden peg using some modifications and sucrose.
Later the Cubensis produced white-rot on T-Gel plain, (no malt, no sugar, no starch).

       

       

       

       




--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineFerather
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Re: Cultivation General Discussion [Re: Ferather] * 1
    #26328172 - 11/18/19 06:02 AM (4 years, 2 months ago)

More info:

Bacterial Growth and Morphology Found in Tea (Camellia sinensis).
Oxidative Stress and Antioxidant Defense.
Reactive Oxygen Species.

----

https://www.shroomery.org/forums/showflat.php/Number/25150722#25150722

----

Experimental substrate (small test sample), fruited in an high oxygen environment (with decay test).

       

----

The above spawn (T-Gel wedge), mixed, the surface growth bruised the most.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (11/18/19 06:30 AM)


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Offlinemcnugget
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Registered: 06/22/13
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Re: Cultivation General Discussion [Re: Ferather]
    #26328205 - 11/18/19 06:53 AM (4 years, 2 months ago)

This morning I noticed that the beads of moisture on the surface of this tub were gone.  I gave it a very light misting and moved it to a spot with less airflow.

In the last 24 hours or so these small white dots started appearing,  hoping these are knots and not some mold making it's foothold. 





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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Cultivation General Discussion [Re: Ferather] * 1
    #26328220 - 11/18/19 07:11 AM (4 years, 2 months ago)
Log in to view attachment

Injury‐Triggered Blueing Reactions of Psilocybe β€œMagic” Mushrooms

they've found the proteins that turn psilocybin into psilocin, and psilocin into blue stuff. it's a polymerization reaction, meaning psilocin molecules link together and turn blue.
they made no mention of any relationship to active alkaloid concentration.
they concluded the function of the reaction in nature, as a defense mechanism against insect attack.


--------------------
 
the j stands for jesus.
2020 new years grow along


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Offlinefootpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: J. Jack Flash]
    #26328226 - 11/18/19 07:16 AM (4 years, 2 months ago)

So the blue is literally just a visual 'fuck off' response?
Or the combined molecules create some sort of toxin/repellent that just happens to be blue?


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OfflineFerather
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Re: Cultivation General Discussion [Re: J. Jack Flash]
    #26328237 - 11/18/19 07:27 AM (4 years, 2 months ago)

@ J. Jack Flash

You should read all of it, try the 3rd to last page, where it talks about ROS, enzymes (such as laccase and peroxidases) and anti-oxidants.
Not much is mentioned about the pesticide effects, although many materials do have these effects on insects.

Tarragon oyster for example, produces coumarin and linalool, which can also effect insects.
That being said, oyster mycelium trap and kill nematodes for nitrogen.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisibleBph
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26328309 - 11/18/19 08:21 AM (4 years, 2 months ago)

Quote:

GypsyCurse said:
Alright, im done with this shit for a while, for several reasons.
Imma spawn the rest of these 50 jars, harvest the fruits, and fuck off.

Being a minimalist/gypsy i refuse to lug shit around so i guess imma bundle up my vacuum sealer, plates, dehydro, 23qt presto, coir, lighting, etc and sell it in the marketplace.




Goonies Never Say Die! But when it's time to break it's time to break. If you feel the need to shed your skin I get that but think twice about selling your goods. I mean it's not that big a deal you can just buy it again when the bug bites you again. Man I was looking forward to your future ape grows. I hope you still visit us at the shroomery if you do hang up your PC for good.


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Offlineverum subsequentis
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Registered: 03/22/16
Posts: 8,732
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Re: Cultivation General Discussion [Re: mcnugget]
    #26328765 - 11/18/19 12:31 PM (4 years, 2 months ago)

Quote:

mcnugget said:
This morning I noticed that the beads of moisture on the surface of this tub were gone.  I gave it a very light misting and moved it to a spot with less airflow.

In the last 24 hours or so these small white dots started appearing,  hoping these are knots and not some mold making it's foothold. 








Looking good and beginning to knot up. Be calm, my friend. you got this!


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: verum subsequentis] * 1
    #26329019 - 11/18/19 02:52 PM (4 years, 2 months ago)

Yeah dude... best advice I can give you(any freshie) is BREATHE HOLMES!!! Fuckers just grow bro... Happens in nature all the time, you not even watching! Lol, I give you a little shit, there's a BIT more too indoor cult, but yeah... Let it go!


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Bph]
    #26329063 - 11/18/19 03:09 PM (4 years, 2 months ago)

Quote:

Bph said:
Quote:

GypsyCurse said:
Alright, im done with this shit for a while, for several reasons.
Imma spawn the rest of these 50 jars, harvest the fruits, and fuck off.

Being a minimalist/gypsy i refuse to lug shit around so i guess imma bundle up my vacuum sealer, plates, dehydro, 23qt presto, coir, lighting, etc and sell it in the marketplace.




Goonies Never Say Die! But when it's time to break it's time to break. If you feel the need to shed your skin I get that but think twice about selling your goods. I mean it's not that big a deal you can just buy it again when the bug bites you again. Man I was looking forward to your future ape grows. I hope you still visit us at the shroomery if you do hang up your PC for good.




Ok ok god damnit lol i have 1 more Ape tub and 3 PE tubs to spawn still. My supplies went to a friend. Everything is available for me when i circle back to babylon. I really shouldn't have even said anything. Was jst way frustrated and whining like a bitch.
Imma giveaway my prints and swabs pretty soon. When i circle back i wanna jst concentrate on APE and Melmac. Not into this PEUW as it turns out. Only lucy pe pheno we have thus far though.

CAPS!!! Sup fucker!?


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OfflineFailboat
Fuck Up

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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26329070 - 11/18/19 03:10 PM (4 years, 2 months ago)

:hereyougo:


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26329073 - 11/18/19 03:12 PM (4 years, 2 months ago)

What's wrong with PEUW? Ahhh, Same shit different day... ALL (actives)I'm running is PEUW lately... Bioassayed? I need to, but RW is sooo good to me


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26329128 - 11/18/19 03:39 PM (4 years, 2 months ago)

Right? i love my peuw. excited to have the next cycle starting on grains now. This thing's been a solid producer every tub with good potency.


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: LtLurker]
    #26329153 - 11/18/19 03:49 PM (4 years, 2 months ago)

So you've rode the wave? PE potency or nah?


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26329168 - 11/18/19 03:55 PM (4 years, 2 months ago)

yea dude, I think i got a piece before you did actually. It's not really PE potency, but it's a kick above average for regular fruits. and the results are much more weight than i get for other pe-types. Probably mostly my own failing there though.


Edited by LtLurker (11/18/19 03:57 PM)


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: LtLurker]
    #26329179 - 11/18/19 04:02 PM (4 years, 2 months ago)

I've not bioassayed the PEUW yet... LMAO, it's horrible... Made some RustyWhyte peanut butter robots with hash in them 2 months ago that I still need to try...


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Ask Caps! (My Q&A Thread)
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OfflineFailboat
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26329183 - 11/18/19 04:04 PM (4 years, 2 months ago)

Oooh mush/hash edibles be jazzy:crazy2:


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InvisibleJ. Jack Flash
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Registered: 11/20/13
Posts: 1,500
Re: Cultivation General Discussion [Re: Ferather]
    #26329253 - 11/18/19 04:48 PM (4 years, 2 months ago)

Quote:

Ferather said:
@ J. Jack Flash

You should read all of it, try the 3rd to last page, where it talks about ROS, enzymes (such as laccase and peroxidases) and anti-oxidants.
Not much is mentioned about the pesticide effects, although many materials do have these effects on insects.

Tarragon oyster for example, produces coumarin and linalool, which can also effect insects.
That being said, oyster mycelium trap and kill nematodes for nitrogen.




i had read it start to finish.

(throughout the paper, "1" refers to psilocybin, "2" refers to psilocin.)

Even
though 2 is not the biosynthetic precursor of 1, the kinase PsiK
can rephosphorylate 2 to 1.[2] Thus, PsiK adds another layer of
self-protection by its capacity to remove 2. Oligo- and polymeric
2 consists of an extended conjugated system and, in the
reduced state, numerous hydroxy groups that are oxidationprone.
Strikingly, these structure elements are similar to those of
anti-oxidative compounds, such as flavonoids and polyphenolic
tannins.[23] In the basic and oxidative environment of insect guts,
such compounds act as producers of reactive oxygen species
and can thus create intestinal lesions which is presumably their
main mode of toxic action.[24] Therefore, polymeric 2 may serve a
defense function.


--------------------
 
the j stands for jesus.
2020 new years grow along


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: J. Jack Flash]
    #26329305 - 11/18/19 05:10 PM (4 years, 2 months ago)

Y'all overcomplicating shit... Eat some shit, SEE SOME SHIT!!!


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