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OfflineA.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
Re: Cultivation General Discussion [Re: Edmunter]
    #23903151 - 12/07/16 12:15 PM (7 years, 1 month ago)

The agar I am working with today.
I know the one is bacterial, other than that any favorites from experienced cultivators?
Thanks
Dick


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"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Cultivation General Discussion [Re: A.RichardTrickle]
    #23903232 - 12/07/16 12:44 PM (7 years, 1 month ago)

Damn that blows!
I have all my plates on a shelf over my monotub. One day I was checking the monotub and when I stood up I smacked my head on the shelf. It lifted up and my stacks of plated all came crashing down. :facepalm3: Luckily it was well after most wedges had already latched on to the agar.


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleComebackKid
Multispore Enthusiast
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Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Cultivation General Discussion [Re: A.RichardTrickle]
    #23903241 - 12/07/16 12:47 PM (7 years, 1 month ago)

The growth in pic 1 is pretty sexy. Feels like I haven't had any rhyzo growth in ages after my last batch of agar.
I definitely haven't had any proper rhyzo growth like that yet either. Hope my new PDA recipe puts out


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
Re: Cultivation General Discussion [Re: ComebackKid]
    #23903245 - 12/07/16 12:47 PM (7 years, 1 month ago)

Damn.....that really sucks.

I knocked over a freshly poured sleeve of dishes a few weeks ago.  Used them anyway lol, although I did lose a couple


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleMoabfighter
Tam Fighter
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Registered: 12/13/15
Posts: 2,710
Re: Cultivation General Discussion [Re: Kenetic]
    #23903247 - 12/07/16 12:48 PM (7 years, 1 month ago)

Wanting to do some SAB and agar work but it's hard with little man running around like a maniac. My wife and I work opposite shifts, I usually have a small few hour window to work on Saturday lol.


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KSSS And PE WBS.
 


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: Moabfighter] * 1
    #23903259 - 12/07/16 12:53 PM (7 years, 1 month ago)

never too early to start learning.


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Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: Cultivation General Discussion [Re: ComebackKid]
    #23903260 - 12/07/16 12:53 PM (7 years, 1 month ago)

How long can you keep a Liquid Innoculant for on the shelf and expect the same results?

I mean, its essentially the same as an LC without any added sugar, right? It has some nutrients from the agar.

Sterilized water, and a clean agar culture, with proper sterile technique should leave you with a clean culture suspended in water. But I feel like I've read experienced cultivators recommend using all of the Li at once, as it doesn't store well. But why? LC can be stored and used for a great amount of time if kept clean.

Can I keep left over Li around for a couple weeks and use it as innoculant without worrying or what's up?


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:vaped: Highwalker :bongload:


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903280 - 12/07/16 12:59 PM (7 years, 1 month ago)

I use LI for long term storage. I've got LIs in my fridge from more than a year ago. IME they store better than slants. On the shelf I usually use them within a few weeks.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903282 - 12/07/16 12:59 PM (7 years, 1 month ago)

I just dont see any point in storing LI. A couple weeks is more than enough time to make new plates for fresh LI.

Also per the tek, u should only be making enough for the # of jars u have. If u have leftovers, thats just extra water u shouldnt have added because it essentially weakened what u did pour.

I know it takes practice to get the pour amount down, but if u have a little extra just pour it all in your last jar. It will explode with growth and reach 100% in like 4-5 days.


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: Munchauzen]
    #23903305 - 12/07/16 01:03 PM (7 years, 1 month ago)

I make my LI in 1/2 pint blender jars. Each jar makes 3 60cc syringes. Usually I only need 1 per batch of inoculations. That leaves me with 2 syringes I can use for the next couple batches without having to sterilise more water.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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InvisibleJosex
#cheat_code
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Registered: 11/13/15
Posts: 8,995
Loc: Flag
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903318 - 12/07/16 01:06 PM (7 years, 1 month ago)

But why would you store a LI for two weeks? You can keep the agar culture in the fridge to stall its growth until you can use it to inoculate and make a fresh LI.

I don't see any problems in what you suggest taking that your agar culture is really clean and doesn't even have a trace of bacteria, but if the culture happens to hide the tiniest amount of bacteria you'd be aiding its fast reproduction by changing to an aqueous media and keeping it stored for 2 weeks.


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Cultivation General Discussion [Re: Josex]
    #23903325 - 12/07/16 01:09 PM (7 years, 1 month ago)

Why bother making LI twice when you can make a few weeks worth all at once.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
Posts: 4,389
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Last seen: 5 years, 3 months
Re: Cultivation General Discussion [Re: MudaFuka]
    #23903333 - 12/07/16 01:11 PM (7 years, 1 month ago)

Gotta say im a big fan of the blenderless tek.  Its my preferred method.  Honestly I think it works better if left to sit for a while. The agar gets soggy and the myc really starts to grow.  Ive got a couple jars of li left from a couple weeks ago and they look fantastic


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: Cultivation General Discussion [Re: Munchauzen]
    #23903334 - 12/07/16 01:13 PM (7 years, 1 month ago)

Awesome, thank you both! Good shit.

And yeah I know munch, I used the whole jar of Li on 10 jars of grain last time so I made up Li for that but then decided to only do 5 jars of each culture so I ended up with leftovers. I was actually worried I had made the agar too stiff because it didn't break up in my Li very well and when I saw my leftovers I was worried the little splash in the jars wasn't gonna be enough. But 6 days later and my KSSS is almost at 100. APE & Cambo are dragging behind but getting there.

Ah okay maybe that's what I ought to do as well. I've just been using the quart jars thus far and blenderless. For ease. But I should really just rig up a blender set up.. And ahh yes syringes would probably store much safer, and definitely take up less room. Thanks again.

Josex, yeah I've got masters in the fridge, and tons of plates running I could use for new Li. Just was curious if the Li I already have is still useable if I sterilize some new grains. Muda has got me thinking long term now though.


--------------------
:vaped: Highwalker :bongload:


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Cultivation General Discussion [Re: Kenetic]
    #23903341 - 12/07/16 01:14 PM (7 years, 1 month ago)

Anybody got a link to the blender less tek? I'm very happy using a blender but I'm curious.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineTiamo
Trust in LITFA
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Registered: 04/07/16
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Re: Cultivation General Discussion [Re: MudaFuka] * 1
    #23903345 - 12/07/16 01:15 PM (7 years, 1 month ago)



--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
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Last seen: 6 years, 22 days
Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903347 - 12/07/16 01:16 PM (7 years, 1 month ago)

In Munch's signature (;


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:vaped: Highwalker :bongload:


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903351 - 12/07/16 01:18 PM (7 years, 1 month ago)

Cool.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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Offlinelukehighwalker710
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Last seen: 6 years, 22 days
Re: Cultivation General Discussion [Re: Kenetic]
    #23903352 - 12/07/16 01:18 PM (7 years, 1 month ago)

Quote:

kenetic said:
Gotta say im a big fan of the blenderless tek.  Its my preferred method.  Honestly I think it works better if left to sit for a while. The agar gets soggy and the myc really starts to grow.  Ive got a couple jars of li left from a couple weeks ago and they look fantastic



Does the myc form a layer on top of the water till swirled around? Then looks like


--------------------
:vaped: Highwalker :bongload:


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Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23903361 - 12/07/16 01:22 PM (7 years, 1 month ago)

Also interested in trying out the pp5 blender bottle with blender ball for Li..


--------------------
:vaped: Highwalker :bongload:


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