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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: General Cult Discussion [Re: Failboat]
#26313748 - 11/11/19 01:14 PM (4 years, 2 months ago) |
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tedoro
ToadStool Tender



Registered: 02/06/15
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Re: General Cult Discussion [Re: Failboat]
#26313749 - 11/11/19 01:15 PM (4 years, 2 months ago) |
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Gotcha! Its an agar recipe you speak of. Mine has malt in it (store bought)
So what is being said here, is that a mycelia grown out on a petri with nutrient characteristics similar to the substrate/spawn it will be grown out in is beneficial? And maybe my mycelia is having a hard go if it switching gears from my malt/agar petri to my bags of wheat berries?
Is this accepted knowledge in the forum?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Ferather
Mycological



Registered: 03/19/15
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Loc: United Kingdom
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Re: General Cult Discussion [Re: tedoro]
#26313757 - 11/11/19 01:20 PM (4 years, 2 months ago) |
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There are various species-strains that don't do well on for example malt, but do well on potato dextrose. I have some cyans that stall on malt extract, but do fine on T-Gel + spawn flour.
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verum subsequentis
seeker of truth



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Re: General Cult Discussion [Re: tedoro]
#26313762 - 11/11/19 01:21 PM (4 years, 2 months ago) |
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It's a theory but far from a proven one. I did a bunch of tests with this and never noticed a positive when using similar nutes. I always use malt agar and then introduce that myc to WBS and it does wonderfully if i don't screw the pooch somewhere along the way.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
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Loc: 90+% Rh
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How do you guys prep your wheat?
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Failboat
Fuck Up

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Re: General Cult Discussion [Re: tedoro] 3
#26313779 - 11/11/19 01:26 PM (4 years, 2 months ago) |
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The idea is to select genetics that theive on the media you will be cultivating with. Grains contain starch and so by using agar with starches, such as grain water agar, you will find mycelium that grows well on the grain it is to consume. When you just use Malt you only find myc that likes sugar, which is not what your using for spawn. That doesn't mean that cultures selected on MEA aren't good, or don't like grain, but it's possible that by using GWA you will find cultures that grow much more prolifically on grain.
I prep wheat, rye, and oats by pouring then into a large pot, filling with water then apply heat. Wait until it comes to a rolling boil and then check for doneness. When grains are cooked, but not overcooked remove from heat, strain, and dry until no longer wet, but just saturated.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Quote:
Changa Alchemist said: How do you guys prep your wheat?
https://www.shroomery.org/forums/showflat.php/Number/24175486#24175486
PinPornProducer's wheat prep method 
Works good for me, though you might need to adjust your boiling times slightly but you can check for readiness by checking kernel softness
I let my wheat air dry for a really long time after the cold rinse. I'm talking at least a couple hours in the strainer. If the strainer had more surface area and allowed the wheat to spread out more I might do it for less time but with the strainers I'm using, 2-6 hours of time is really not a big deal. Seems to get it to the perfect moisture level for me. I go by feel of the grain at that point, I like the outside to be dry/nearly dry.
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tedoro
ToadStool Tender



Registered: 02/06/15
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I'd follow teks for rye. My experience is that they are quite similar. My personal belief is that a hard boil for at least 10 minutes is critical for the berries to get broken down enough to colonize well. There is a trap here tho, its very easy to over saturate the berries on the way to that 10 minute boil.... so lots of boiling water to begin with and a powerful stove.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Failboat
Fuck Up

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Re: General Cult Discussion [Re: tedoro]
#26313854 - 11/11/19 01:50 PM (4 years, 2 months ago) |
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Cooking most grain is all the same. Just pay attention.
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spiritlands



Registered: 06/21/10
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Re: General Cult Discussion [Re: Failboat]
#26313890 - 11/11/19 02:04 PM (4 years, 2 months ago) |
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We have a local owned place that has a good bulk food section. It's called WinCo, idk if they're any other states. I ended up doing 2x atl7 and a pan cyan with the wheat. Did my wbs with x7x and GATZ.
Ever since I got my birdseed figured out I keep making bigger and bigger bags of it. Think I'm going about it wrong. Think I should be starting smaller jars and expanding them instead of expecting huge bags to colonize. Ive been getting away with it but I just saw a tiny bit of trich on a sunflower seed in a bag that just hit 100%. Picked up some quart jars today, been using half gallons and filter bags.
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StingingRoger
Stranger
Registered: 11/13/17
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how long does everyone wait for the air in the sab room to settle after turning off computer fans and closing the door to start working ?
thanks
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Edmunter
Mr



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Re: General Cult Discussion [Re: Failboat]
#26313905 - 11/11/19 02:10 PM (4 years, 2 months ago) |
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Quote:
Quirkmeister92 said: The idea is to select genetics that theive on the media you will be cultivating with. Grains contain starch and so by using agar with starches, such as grain water agar, you will find mycelium that grows well on the grain it is to consume. When you just use Malt you only find myc that likes sugar, which is not what your using for spawn. That doesn't mean that cultures selected on MEA aren't good, or don't like grain, but it's possible that by using GWA you will find cultures that grow much more prolifically on grain.
I prep wheat, rye, and oats by pouring then into a large pot, filling with water then apply heat. Wait until it comes to a rolling boil and then check for doneness. When grains are cooked, but not overcooked remove from heat, strain, and dry until no longer wet, but just saturated.
Nice, I might give that theory a side by side.
Do you not soak Rye over night?
I have a culture atm that does this on grains. Its is quite vigorous in the tub, smell great when spawning and im a bit confused why it is doing this. Ive never had it like this before. Anyone had dealings with this before?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: General Cult Discussion [Re: tedoro] 1
#26313906 - 11/11/19 02:11 PM (4 years, 2 months ago) |
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@verum subsequentis
I'm not here to change anything, I did say it's a bit more advanced, and needs steps. Quirkmeister92 pretty much explained it (here). You can domesticate the mycelium from spores (genetics), and select growth (which is less of a gamble in the end).
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[Advanced], for informative purposes.
Here I germinated Cubensis spores on-in a wooden peg using some modifications and sucrose. Later the Cubensis produced white-rot on T-Gel plain, (no malt, no sugar, no starch).



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Failboat
Fuck Up

Registered: 02/01/18
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I like to use pints for masters. Because the sides are smooth the grains tumble right out with minimal tilt. Millet is good for size, but a bit tricky for hydration. Milo is easy to prep and small enough to make for a good master. 1 pint to 4qt/1bag is pretty convenient for quick colonization.
I don't soak any grains overnight.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: General Cult Discussion [Re: Ferather] 1
#26313987 - 11/11/19 02:44 PM (4 years, 2 months ago) |
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Quote:
Ferather said: There are various species-strains that don't do well on for example malt, but do well on potato dextrose. I have some cyans that stall on malt extract, but do fine on T-Gel + spawn flour.

trying this. all of my cyans(pnw cy's) have been stalling on agar lately but leap right off onto grain.
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: General Cult Discussion [Re: TedsDead]
#26313999 - 11/11/19 02:52 PM (4 years, 2 months ago) |
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I trade you data, for data.
"Psilocybe cyanescens grows today primarily on wood chips, especially in and along the perimeter of mulched plant beds in urban areas, but can also grow on other lignin-rich substrates. P. cyanescens does not grow on substrate that is not lignin-rich."
Source (wikipedia), seems to want cellulose + phenolic compounds (wood, wood like materials).
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https://imgur.com/a/8jngxe8
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: General Cult Discussion [Re: TedsDead] 2
#26314000 - 11/11/19 02:53 PM (4 years, 2 months ago) |
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cyans definitely dont like mea IME. but if you make it with woodchip tea it works . pda works too
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: General Cult Discussion [Re: AyePlus]
#26314015 - 11/11/19 03:02 PM (4 years, 2 months ago) |
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: General Cult Discussion [Re: Ferather]
#26314040 - 11/11/19 03:17 PM (4 years, 2 months ago) |
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yes, youre right! a few yrs ago I used woodchip tea for my agar when I did them but not this time just been busy and already had the plates poured. always good info Ferather;) I've been staring at these things for a month going "WTF?" but now Im like "ah...." gonna transfer to new media and see how it goes
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: General Cult Discussion [Re: TedsDead]
#26314083 - 11/11/19 03:35 PM (4 years, 2 months ago) |
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I actually forgot that experiment until you brought it up ferather, it was like 3-4 years ago
also might explain why I had a bitch of a time getting any of my woodlover prints to germinate on MEA this spring, even though my cyan print was only a few weeks old, no contams no nothing, from a dark ass wild print too. shoulda cloned the fruit instead but I spaced it.
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