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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 12 hours
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Re: general cult discussion [Re: Edmunter]
#26312951 - 11/11/19 04:10 AM (4 years, 2 months ago) |
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Hi, i wanted to point out something that im am finding quite disturbing...
For all of you shoeboxers are you aware of the fact that doing bulk at 1:1 to 1:2 ratios will yield aproximately the same (of MAGIC) as the same amount of substrate obtained from a jar of sclerotia?
That would mean that we are not doing a very efficient work here... I mean, sclerotia couldnt be easier and it may or may not take the same time as any other cube out there.
Is there anything i am missing? or are we all doing inefficient cubensis grows? maybe we should all be going higher spawn ratios?
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: general cult discussion [Re: Sankhara] 1
#26313049 - 11/11/19 06:23 AM (4 years, 2 months ago) |
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I have tubs that are stained green in some spots and I still use them just fine
I spray out with a hose and wipe down with a dilute bleach solution before spawning
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meaculpaUIO



Registered: 08/26/19
Posts: 956
Last seen: 4 years, 1 month
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Please help me decide what to do with this jar. Should I just throw it out? Based on what I have read, I either throw this away, or give it FAE in a separate chamber. Its like 10% left of the bottom to colonize.
Its very fluffy at top (wants FAE?) and it has some myc piss/yellow/bacteria/metabolites I dont know what it is.
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 9 hours, 8 minutes
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good morning cult, I tried searching "real petrie" "not no pour" ect in munchs blenderless LI thread but didn't find what I was looking for.
Has anyone got any experience or advice for dropping a whole plate from a pour dish into LI jar vs using a pastyplate?
I have a PE MS culture that I already took transfers from, and wanted to use the rest of the plate for LI.
-------------------- All I need are some tasty waves, a cool buzz, and Iβm fine. Whatever you decide wonβt really impact our survival Close your eyes, and do the best that you can
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 12 hours
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Quote:
meaculpaUIO said:

Please help me decide what to do with this jar. Should I just throw it out? Based on what I have read, I either throw this away, or give it FAE in a separate chamber. Its like 10% left of the bottom to colonize.
Its very fluffy at top (wants FAE?) and it has some myc piss/yellow/bacteria/metabolites I dont know what it is.
You could jar fruit it. It clearly has some sort of contamination and probably wont achieve full colonization. The general consensus is to throw anything contaminated, but if you still want to give it a try i would do so in another chamber
Its metabolites, please, metabolites. You could cause a seizure to many cultivators by saying "myc piss".
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 12 hours
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Re: general cult discussion [Re: Camera93]
#26313101 - 11/11/19 07:07 AM (4 years, 2 months ago) |
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Quote:
Camera93 said: good morning cult, I tried searching "real petrie" "not no pour" ect in munchs blenderless LI thread but didn't find what I was looking for.
Has anyone got any experience or advice for dropping a whole plate from a pour dish into LI jar vs using a pastyplate?
I have a PE MS culture that I already took transfers from, and wanted to use the rest of the plate for LI.
Just slice it in 4 and drop those pieces in, the edges may contain some contaminats though. If you poured really thin it is going to be a struggle probably. I thing pasty plates are better for dropping entire pieces of agar
Next time try searchin for "petri" rather than "petries" or just "plates"
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meaculpaUIO



Registered: 08/26/19
Posts: 956
Last seen: 4 years, 1 month
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Re: general cult discussion [Re: Sankhara]
#26313135 - 11/11/19 07:34 AM (4 years, 2 months ago) |
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Quote:
Sankhara said:
Quote:
meaculpaUIO said:

Please help me decide what to do with this jar. Should I just throw it out? Based on what I have read, I either throw this away, or give it FAE in a separate chamber. Its like 10% left of the bottom to colonize.
Its very fluffy at top (wants FAE?) and it has some myc piss/yellow/bacteria/metabolites I dont know what it is.
You could jar fruit it. It clearly has some sort of contamination and probably wont achieve full colonization. The general consensus is to throw anything contaminated, but if you still want to give it a try i would do so in another chamber
Its metabolites, please, metabolites. You could cause a seizure to many cultivators by saying "myc piss".
Okay. I put the entire jar in a mycobag, moved the bag to a SAB. Opened the Lid in the SAB.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: General Cult Discussion [Re: Edmunter] 1
#26313259 - 11/11/19 09:04 AM (4 years, 2 months ago) |
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Quote:
Edmunter said: Organic rye seems to produce a weaker , duller mycellium. Since switching to Millet I really see the difference. If you G2G from millet to rye it then works much better.
That's pretty much the same result I got. Making agar from your spawn water is also ideal, use coffee filters to make filtered water. Germination, growth, wedges and isolation, will be similar to doing so with the spawn (enzymes, genetics, so on).
Malt extract for example, is the resulting sugars produced from starch + enzymes.
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If you are fairly good with agar, you could also try extracting materials from the end media too (example coir). It's possible to grind up some spawn and coir together, boil it up, then filter it to make agar.
With wood loving mycelium, it was beneficial to have trace wood debris (coir).
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spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
Last seen: 1 month, 28 days
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Re: General Cult Discussion [Re: Ferather]
#26313326 - 11/11/19 09:37 AM (4 years, 2 months ago) |
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Just did a 20m simmer on some wheat berries. Never tried them but found some cheap and clean. Has anyone used anything like this for atl7? I have wbs also but idk if I want to eat birdseed truffles. I'm just experimenting with these grains but I have pans, atl7, gatz cubes, and x7x cubes I could inoculate. Waiting on a PC cycle, what should I do? I'm going to prep some wbs too.
I really like the look of these wheat berries. I've never done rye but these have a great consistency and moisture seems real easy to get right.
Edited by spiritlands (11/11/19 12:18 PM)
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 23 hours, 42 minutes
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Re: General Cult Discussion [Re: Ferather]
#26313327 - 11/11/19 09:38 AM (4 years, 2 months ago) |
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ferather,
This is super interesting to me. I have a few questions...
1) How do you get the softness, hardness of the agar correct when using nutrient water?
2) Is agar the right term at this point?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: General Cult Discussion [Re: tedoro]
#26313472 - 11/11/19 10:42 AM (4 years, 2 months ago) |
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Do you mean grain water after cooking? Unfortunately grain water is not measured (how much you get in the water), and each grain is very different.
When I used flour to boiling hot water, it made a murky solution, but once filtered, it had a mild tint was was mostly transparent. Flour can be measured before you add it to water, for example 8g, however filtering is less measured though.
Most of what you measure as flour will be filtered out as sediment and bran.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
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Re: General Cult Discussion [Re: Ferather]
#26313667 - 11/11/19 12:37 PM (4 years, 2 months ago) |
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I've spent time with my agar mix to get it just soft enough to blend into LI easily, yet hard enough to make ropey growth. Seems challenging to achieve with flour water. But I guess if you document your steps....
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 55 minutes
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Re: General Cult Discussion [Re: Ferather]
#26313673 - 11/11/19 12:43 PM (4 years, 2 months ago) |
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I used grain water and made agar so strong that my oysters haven't reched the edge after like a month. Tedoro, Agar is agar and agar is what makes the broth solidify so using grain water doesn't change how much agar one uses in the solution.
Spiritlands, I have some Tamps forming stones on wheat currently. They're a great choice. Basically avoid oats for stones becausethe hulls often are stuck throughout the sclerotia. If you have choices, then I reccomend medium sized grains like rye or wheat, although I have very impressive Cincts Warrens on Milo/Sorghum.
Edited by Failboat (11/11/19 12:56 PM)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
spiritlands said: Just did a 20m simmer on some wheat berries. Never tried them but found some cheap and clean. Has anyone used anything like this for atl7? I have wbs also but idk if I want to eat birdseed truffles. I'm just experimenting with these grains but I have pans, atl7, gatz cubes, and x7x cubes I could inoculate. Waiting on a PC cycle, what should I do? I'm going to prep some wbs too.
I really like the look of these wheat berries. I've never done rye but these have a great consistency and moisture seems real easy to get right.
Yo spirit I've used wheat berries for cubes it's by far my favorite grain. I've never used for sclerotia producing species though. Another member Boogie also used to use wheat from a feed store. I've only ever used food grade myself. Stopped using it because of price. It's a pretty good grain spawn and I'm sure it will work fine for atl.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: General Cult Discussion [Re: Failboat]
#26313694 - 11/11/19 12:54 PM (4 years, 2 months ago) |
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Quote:
Quirkmeister92 said: I used grain water and made agar so strong that my oysters haven't reched the edge after like a month. Tedoro, Agar is agar and agar is what makes the broth solidify so using grain water doesn't change how much agar one uses in the solution.
Spiritlands, I have some Tamps forming stones on wheat currently. They're a great choice. Basically avoid oats for stones becausethe hulls often are stuck throughout the sclerotia. If you have choices, then I reccomend medium sized grains like rye or wheat, although I have very impressive Cints Warrens on Milo/Sorghum.
Quirk where do you get your wheat from?
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 55 minutes
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Re: General Cult Discussion [Re: gizmo1]
#26313696 - 11/11/19 12:55 PM (4 years, 2 months ago) |
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I got it from the local feed store. Maybe ~$15/50#
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: General Cult Discussion [Re: Failboat]
#26313709 - 11/11/19 01:00 PM (4 years, 2 months ago) |
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Quote:
Quirkmeister92 said: I got it from the local feed store. Maybe ~$15/50#
Is it just labeled as wheat?
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: General Cult Discussion [Re: gizmo1]
#26313720 - 11/11/19 01:07 PM (4 years, 2 months ago) |
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The stuff I buy at the feed place here is called whole wheat on the bag
(this is in Ontario, Canada)
https://www.tscstores.com/25KG-TSC-WHOLE-WHEAT-P29163.aspx

For any other Canadians looking, if you have TSC stores around you, this is the best deal I've found on grains. Under $13 CAD for 25kg . If you have a distance to drive it's worth calling the store to ask if they have stock. If not they'll order it for you.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: General Cult Discussion [Re: Failboat] 1
#26313739 - 11/11/19 01:10 PM (4 years, 2 months ago) |
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You seem to have grasped the idea, it's a bit more advanced, but so far results are better. Spores get to germinate and express genetics more suitable to what you have (in terms of spawn). Everyone will be using different grain-seed-mixes, even the coco coir you use will vary (even from the same vendor), adding the coir however is a further step.
Most phenols, phenolic compounds, are acidic, and may require partial neutralization using calcium (lime, CaCO3), allowig the agar to set. The calcium powder can be added with the flour and coir powder, prior to mixing with water, and filtering.
Starch lumps are soft when hydrated and cooked, no different to 'very cooked grain'.
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http://agargel.com.br/en/agar-agar/#especificacoes
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 55 minutes
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Re: General Cult Discussion [Re: Ferather] 2
#26313741 - 11/11/19 01:11 PM (4 years, 2 months ago) |
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I got winter wheat. Told the lady who runs the place what it was for when she asked if I was trying to grow the grains themselves. She had never heard about growing mushrooms. Blew her mind lol. Gonna take her some of these oysters when I get em fruiting. Just nocced up a dozen jars or so of blue and wild white plus some Shiitake. All came from Caps.
Edited by Failboat (11/11/19 01:14 PM)
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