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InvisibleBoogieman47
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Registered: 03/05/16
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Re: general cult discussion [Re: Edmunter]
    #26301970 - 11/06/19 12:47 AM (4 years, 2 months ago)

I try to get them sooner these dont drop many spores so I can wait longer .. but they usually wait till early morning to finish up Haha and the rest I'm just too lazy to get to em


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InvisiblemushboyMDiscord
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Re: general cult discussion [Re: Boogieman47]
    #26301978 - 11/06/19 12:58 AM (4 years, 2 months ago)

So lazy
:creepylurker:


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OfflineEdmunter
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Re: general cult discussion [Re: mushboy]
    #26302164 - 11/06/19 05:58 AM (4 years, 2 months ago)

Can someone show me a colonised millet jar to compare.........


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OfflineGan
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Re: general cult discussion [Re: LtLurker]
    #26302227 - 11/06/19 06:56 AM (4 years, 2 months ago)

Quote:

LtLurker said:
i don't know how better to describe it and i can't really do the pic edit thing some do. There's like a big water spot taking most of the plate that you can clearly see when held up to light. If you look close at the right angle it probably won't look like normal smooth agar, it'll be "choppy".





Is part of it this darkened, water stained looking section of myc that I have outlined in red here?

Or is it more of a whole plate deal, even the portions outside the myc? Sorry for being such a pain in the ass, but when I look at it in person I just can't see the indicators for contam, even though I know its contaminated lol. I really appreciate the advice thus far though Lurker. Anyone else chime in if you think you can help myesee what I should be seeing :facepalm3:


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OfflineCamera93
We got dicks like Jesus
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Registered: 08/15/18
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Re: general cult discussion [Re: Gan]
    #26302234 - 11/06/19 07:01 AM (4 years, 2 months ago)

from the pic, it looks safer up top

looks like a film all under the culture and a lot of it around the bottom

like Lurker said, how does the myc look viewed from the side/angle? is it laying nice and flat or choppy like little waves like he asked?


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineGan
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Re: general cult discussion [Re: Camera93]
    #26302252 - 11/06/19 07:21 AM (4 years, 2 months ago)

Quote:

Camera93 said:
from the pic, it looks safer up top

looks like a film all under the culture and a lot of it around the bottom

like Lurker said, how does the myc look viewed from the side/angle? is it laying nice and flat or choppy like little waves like he asked?




I wasn't able to get it to an angle where I could see the choppiness of the agar, but that doesn't mean it's not there. I went ahead and did a hot pour on top of some of the plates that looked like that. I'm still not able to clearly see the film, other than the agar looks a little rough/not as transparent in some places. Not sure if that's what you all are talking about. I think its more just having newer eyes to spotting contams, not your all's descriptions. I'll have to keep learning to spot it. Thanks for all the help/advice!


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OfflineCamera93
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Re: general cult discussion [Re: Gan]
    #26302295 - 11/06/19 07:51 AM (4 years, 2 months ago)

Quote:

Gan said:
Quote:

Camera93 said:
from the pic, it looks safer up top

looks like a film all under the culture and a lot of it around the bottom

like Lurker said, how does the myc look viewed from the side/angle? is it laying nice and flat or choppy like little waves like he asked?




I wasn't able to get it to an angle where I could see the choppiness of the agar, but that doesn't mean it's not there. I went ahead and did a hot pour on top of some of the plates that looked like that. I'm still not able to clearly see the film, other than the agar looks a little rough/not as transparent in some places. Not sure if that's what you all are talking about. I think its more just having newer eyes to spotting contams, not your all's descriptions. I'll have to keep learning to spot it. Thanks for all the help/advice!





I have yet to try the hot pour, how did the process go?
just literally pouring liquid agar over a plate?


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineGan
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Re: general cult discussion [Re: Camera93]
    #26302351 - 11/06/19 08:33 AM (4 years, 2 months ago)

Quote:

Camera93 said:
I have yet to try the hot pour, how did the process go?
just literally pouring liquid agar over a plate?




Yeah it was pretty much that. I let it get down to the temperature I normally pour plates at (~120F), but I read that others sometimes like for it to be a bit hotter than pour temperature. I think I may have poured it too thick, because I poured it on top of each culture until it covered the whole culture/plate. Since the culture is slightly raised above the surface of the agar, it required a fairly thick layer to cover the whole culture completely. Not sure if I'm supposed to cover the whole culture, or just parts of it.

The hot pour layer was roughly as thick as I pour a normal plate, but on top of an already poured plate and culture. There was still a decent amount of clearance space between the top of the second layer and the top of the petri. We'll see how it does :shrug:


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Offlinespiritlands
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Re: general cult discussion [Re: Gan]
    #26302372 - 11/06/19 08:47 AM (4 years, 2 months ago)

When I hot pour I cover the whole plate so there's a brand new surface, contam free hopefully. If I don't have clearance or don't want to hit the top I'll pour enough so when I tilt it it at least covers the surface of the old agar. This way is not super thick but still provides a barrier to grow through.

And I like it pretty hot.
:monkeyspank:


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineGan
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Re: general cult discussion [Re: spiritlands]
    #26302392 - 11/06/19 08:56 AM (4 years, 2 months ago)

Quote:

spiritlands said:
When I hot pour I cover the whole plate so there's a brand new surface, contam free hopefully. If I don't have clearance or don't want to hit the top I'll pour enough so when I tilt it it at least covers the surface of the old agar. This way is not super thick but still provides a barrier to grow through.

And I like it pretty hot.
:monkeyspank:




I'll have to try tilting it next time if I have to do it again. Do you find hot pour works decently well on bacterial dishes for you? Also, when you transfer the new myc away from the contam free surface, do you actually cut into the agar like a transfer, or just scrape some off the top?

Also, I'll try it a little hotter next time  :eatingout:


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OfflineEdmunter
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Re: general cult discussion [Re: Gan]
    #26302528 - 11/06/19 10:03 AM (4 years, 2 months ago)

Does anyone use just millet for everything?


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Offlinethe_sonic16
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Registered: 03/10/19
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Re: general cult discussion [Re: Edmunter]
    #26302549 - 11/06/19 10:11 AM (4 years, 2 months ago)

(This is the nerdiest thing I'll say today). Yes, a guy in my mycology club does. It seems to work for him.


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Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: general cult discussion [Re: Boogieman47]
    #26302576 - 11/06/19 10:25 AM (4 years, 2 months ago)

Quote:

Boogieman47 said:


Here you go my friend



Thx Boog! Oh wow yeah see, they look like normal cubes.

Edmunter - I use white millet for everything atm.


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Offlinespiritlands
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Re: general cult discussion [Re: Gan]
    #26302621 - 11/06/19 10:49 AM (4 years, 2 months ago)

Quote:

Gan said:
Quote:

spiritlands said:
When I hot pour I cover the whole plate so there's a brand new surface, contam free hopefully. If I don't have clearance or don't want to hit the top I'll pour enough so when I tilt it it at least covers the surface of the old agar. This way is not super thick but still provides a barrier to grow through.

And I like it pretty hot.
:monkeyspank:




I'll have to try tilting it next time if I have to do it again. Do you find hot pour works decently well on bacterial dishes for you? Also, when you transfer the new myc away from the contam free surface, do you actually cut into the agar like a transfer, or just scrape some off the top?

Also, I'll try it a little hotter next time  :eatingout:



TBH bacterial contams I just transfer away from, they aren't fast movers like something generating spores. But yes it stops them in their tracks. Mold contams are the problem though bc surviving mold Mycelium can burrow through the agar. Bc of this I try to just scrape tissue off the surface. I try to pour smooth so that any contams spores will get covered by agar without becoming air borne. Also why I try to coat as much of the old surface as I can including the walls. This is just how I do it there may be better ways.
:ancientaliens:


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineEdmunter
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Re: general cult discussion [Re: spiritlands]
    #26304931 - 11/07/19 11:59 AM (4 years, 2 months ago)

Quick germination   



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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: general cult discussion [Re: Edmunter]
    #26305655 - 11/07/19 04:57 PM (4 years, 2 months ago)

Opened up the PEuW tub today and caught a whiff of hot fart. Dnt knw what's up with that.
I also label my peuw shit as LPE even though it ain't really lucistic penis envy. Had me thnkin.

Is there a Lucistic Penis Envy?


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InvisibleBoogieman47
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Re: general cult discussion [Re: Feasoghorm]
    #26305656 - 11/07/19 04:57 PM (4 years, 2 months ago)

There will be soon after I get done with this experiment with cron


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: general cult discussion [Re: Boogieman47]
    #26305668 - 11/07/19 05:03 PM (4 years, 2 months ago)

Dont lucistics jst pop up at random like albinos?
Ya cant make one, no?


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InvisibleBoogieman47
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Re: general cult discussion [Re: Feasoghorm]
    #26305671 - 11/07/19 05:04 PM (4 years, 2 months ago)

You can cross it with an already Lucy fruit
. Such as an aa+or lgt


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Offlinejbgtaa
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Re: general cult discussion [Re: Boogieman47]
    #26305716 - 11/07/19 05:25 PM (4 years, 2 months ago)

Spawning some LGT I got from you tomorrow gypsy, super fucking excited considering my main working culture has been standard GT, it will be awesome to give my friends the same species just a different color


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If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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