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OfflineSankhara
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Re: Cultivation General Discussion [Re: chipsandwich]
    #26232962 - 10/06/19 05:53 AM (4 years, 3 months ago)

Quote:

chipsandwich said:

Do you think this single layer of tyvek will be sufficient?




AFAIK tyvek is the worst you could have chosen to use. I use micropore, and that is still not acceptable for many trusted cultivators.

Have zero experience with it though, just my 2 cents


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Sankhara]
    #26232970 - 10/06/19 06:11 AM (4 years, 3 months ago)

Quote:

Sankhara said:
Quote:

chipsandwich said:

Do you think this single layer of tyvek will be sufficient?




AFAIK tyvek is the worst you could have chosen to use. I use micropore, and that is still not acceptable for many trusted cultivators.

Have zero experience with it though, just my 2 cents




3 laters of micropore tape is better


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InvisibleJHOVA
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Re: Cultivation General Discussion [Re: Edmunter]
    #26232983 - 10/06/19 06:34 AM (4 years, 3 months ago)

No way. One layer of tyveek secured by mp is better than 3 micropore.


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InvisibleYesum
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Re: Cultivation General Discussion [Re: JHOVA]
    #26233050 - 10/06/19 07:58 AM (4 years, 3 months ago)

Quote:

JHOVA said:
No way. One layer of tyveek secured by mp is better than 3 micropore.




I'd say there about equal.  I use both approaches. Both lid setups seem to work fine


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InvisibleJust_A_Noob
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Re: Cultivation General Discussion [Re: JHOVA]
    #26233071 - 10/06/19 08:12 AM (4 years, 3 months ago)

Not with the duct tape lol. 

Quote:

JHOVA said:
No way. One layer of tyveek secured by mp is better than 3 micropore.




:whathesaid:


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Just_A_Noob] * 1
    #26233112 - 10/06/19 08:38 AM (4 years, 3 months ago)

mp can work, people have used it in single and multiple layers and got away with it. But tyvek should be better since it's a finer filter(smaller openings) Multiple layers of a larger pore filter shouldn't be better than a single layer of a finer pore filter.

Maybe to phrase it a little clearer(but making up microns) 3 layers of a 10micron filter will still just be 3x 10micron filters, they won't/shouldn't filter as well as a single layer of 5micron filter.

More importantly though, is that even tyvek? It looks like some sort of thick weaved material. If i'm seeing the pic right, it looks like you can see right through it, that doesn't look right to me.


Edited by LtLurker (10/06/19 08:43 AM)


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Invisiblesh4d0ws
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Re: Cultivation General Discussion [Re: LtLurker]
    #26233243 - 10/06/19 10:04 AM (4 years, 3 months ago)

Yeah what kind of tyvek is that (where did you get it?)? The green stuff is tyvek??


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OfflineWhiteboywasted
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #26233246 - 10/06/19 10:07 AM (4 years, 3 months ago)

Quick question if i have 2 tiny mold spots on a brf jar that is almost fully colonized can i birth it and cut away the mold?


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OfflineSankhara
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Re: Cultivation General Discussion [Re: Whiteboywasted]
    #26233478 - 10/06/19 12:29 PM (4 years, 3 months ago)

So... i was thinking about how pan cyans love dung. And what consitutes dung, and couldnt help but wonder if they would fruit on fermented substrate without dung. For example fermenting rye with any ferment for a few days, adjusting ph and then pc the grains.

Poop its just decomposed material, maybe they benefit from predigested food sources that dont necessarily have to br dung.

Just an idea... dont even have experience with them, but it seems very plausible


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Offlinepixelpopper
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Re: Cultivation General Discussion [Re: Sankhara]
    #26233484 - 10/06/19 12:34 PM (4 years, 3 months ago)

Regarding watching used listings for equipment, you can also setup alerts for ebay

If you do a search, you can narrow it down to price ranges and any other modifiers you want. You can also specify in the search to exclude certain terms by adding "--dontincludethisterm" in the search. Then you click on "save this search" and it will email you whenever new listings pop up matching your criteria.

If you wait long enough, you can find all kinds of stuff for super cheap... sometimes people list things with crappy titles so nobody sees the listing, so you may want to be creative in how you're searching.

Although its better to find used stuff locally because then you don't have to pay for shipping


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: ChardRich]
    #26233504 - 10/06/19 12:48 PM (4 years, 3 months ago)

Quote:

ChardRich said:
It wasn't super grown out yet, so that's probably a contributing factor.




You also said in another thread that you didn't see growth in 24 hrs and decided to shake the jar, right? This, in my opinion, was a bad idea. What was your logic in doing that?

I haven't figured out exactly what i think about this yet but have done several tests regarding violently shaking freshly pour LC jars and bags vs gently rolling vs no shaking or rolling and have observed some interesting things. It seems that the Myc that is growing all by itself is far more fragile and sensitive than in a colonized grain jar.

My theory, as it stands, is that Myc is rather shy and fragile when not protected by something. For example, we all know that the myc disappears when a grain jar is shaken but readily shows back up. I believe that this is because the Myc on the inside of the grain is not damaged and therefore can bounce back rapidly.

My test with shaking LC jars and bags have quite consistently shown that an aggressive shake greatly slows down the bounce back and that a gentle roll typically doesn't speed things up. I just pour in the LC and let it sit.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26233532 - 10/06/19 12:59 PM (4 years, 3 months ago)

Quote:

verum subsequentis said:
Quote:

ChardRich said:
It wasn't super grown out yet, so that's probably a contributing factor.




You also said in another thread that you didn't see growth in 24 hrs and decided to shake the jar, right? This, in my opinion, was a bad idea. What was your logic in doing that?

I haven't figured out exactly what i think about this yet but have done several tests regarding violently shaking freshly pour LC jars and bags vs gently rolling vs no shaking or rolling and have observed some interesting things. It seems that the Myc that is growing all by itself is far more fragile and sensitive than in a colonized grain jar.

My theory, as it stands, is that Myc is rather shy and fragile when not protected by something. For example, we all know that the myc disappears when a grain jar is shaken but readily shows back up. I believe that this is because the Myc on the inside of the grain is not damaged and therefore can bounce back rapidly.

My test with shaking LC jars and bags have quite consistently shown that an aggressive shake greatly slows down the bounce back and that a gentle roll typically doesn't speed things up. I just pour in the LC and let it sit.




How long would you expect to see growth after inoculating with 3cc’s of LI made in a blender and shaken at inoculation?  Been about 3 days not seeing anything yet. Been a couple years since I did any culture work with liquids and I’m feeling rusty AF

Also: when testing LC/LI on agar what is your preffered method? And how long do you wait before you consider a test successful

I used a flamed inoculation loop to try to transfer some myc from the LC to the agar and it kinda worked but I feel like there has to be an easier way because the myc stuck to the loop and I had to smash it through the agar to dislodge a little bit, looks like it worked on 3/4 of the tests i did 2 days ago but the recovery from LI/LC always seems a little off to me even when its clean.

When I’m was using syringes for LC I would shake the shit out of it then fill a syringe and put a drop on each plate before cappingn and wrapping, that was legit but I’ve been playing with using this SS inoculation gun instead of individual syringes and that makes my old method obsolete. Any pointers?


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OfflineChardRich
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26233580 - 10/06/19 01:18 PM (4 years, 3 months ago)

Quote:

verum subsequentis said:
Quote:

ChardRich said:
It wasn't super grown out yet, so that's probably a contributing factor.




You also said in another thread that you didn't see growth in 24 hrs and decided to shake the jar, right? This, in my opinion, was a bad idea. What was your logic in doing that?

I haven't figured out exactly what i think about this yet but have done several tests regarding violently shaking freshly pour LC jars and bags vs gently rolling vs no shaking or rolling and have observed some interesting things. It seems that the Myc that is growing all by itself is far more fragile and sensitive than in a colonized grain jar.

My theory, as it stands, is that Myc is rather shy and fragile when not protected by something. For example, we all know that the myc disappears when a grain jar is shaken but readily shows back up. I believe that this is because the Myc on the inside of the grain is not damaged and therefore can bounce back rapidly.

My test with shaking LC jars and bags have quite consistently shown that an aggressive shake greatly slows down the bounce back and that a gentle roll typically doesn't speed things up. I just pour in the LC and let it sit.




Agreed that it was probably not a great idea. I guess after two days no growth I figured something was wrong and it was already fucked?

Another LC I made with more nutes (my scale fucked up on the one that's not germinating) is already showing growth after like 36 hours.


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: ChardRich]
    #26233588 - 10/06/19 01:24 PM (4 years, 3 months ago)

AYE, my preferred method is a flame sterilized loop, dipped in the LC and streaked (struck?) across a plate. I can generally tell if it's clean in two or three days but waiting longer makes it more obvious.

Yes, LC recovery on agar can often look quite different than a clean culture from an A2A transfer. I think this is because it is often myc growing on myc growing on myc. This results in rather fluffy growth and can be a little tricky to identify as clean at first. After a dozen or so goes at it you can learn to tell if it's clean even though it looks a little odd.

Also, a good thing to do is to streak the plate and watch the areas of the streak that have no obvious myc growth. Bacteria will generally be very obvious along this line.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: ChardRich]
    #26233589 - 10/06/19 01:25 PM (4 years, 3 months ago)

Imma say your lc was contaminated.

Slow growth in the broth + poor recovery on grains?

:justno:
Nutes shouldn't matter. I've used a 'pinch' to 6g per 500ml(like agar)

Still grew shrooms


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OfflineChardRich
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Re: Cultivation General Discussion [Re: mushboy]
    #26233600 - 10/06/19 01:31 PM (4 years, 3 months ago)

I agree with you. Especially since my other LC is already showing growth in jars and I made it a week later :lol:

It's cool, the culture I used wasn't even that great. I have lots of nice plates I will go ahead and make a new one with  :runaway:


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: ChardRich]
    #26233613 - 10/06/19 01:44 PM (4 years, 3 months ago)

Im gunna make a LC this week for shiitake and oysters.  Best to test first b4 spoiling loads of jars/bags


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Edmunter]
    #26233624 - 10/06/19 01:52 PM (4 years, 3 months ago)

I think so.


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OfflineChardRich
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Re: Cultivation General Discussion [Re: Edmunter]
    #26233626 - 10/06/19 01:53 PM (4 years, 3 months ago)

I just prep a bunch of jars and test on one, doesn't really matter tho :shrug:


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: ChardRich]
    #26233680 - 10/06/19 02:21 PM (4 years, 3 months ago)

Thanks V,

Thats pretty much exactly what I did, I dont use a stir plate so lc was a little chunky making it a bit more awkward, should have spread it a little more but seeing a little myc recovery and no bacteria after 2 days probably means I’m good to cook some grain today eh?

Any thoughts on typical recovery for a blender LI? I havent looked at every jar but only seeing growth in 1/5 I looked at (noc’d 10/3), probably just being impatient though.


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Learn about breeding

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Read more, post less.
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