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OfflineCamera93
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Re: Cultivation General Discussion [Re: Edmunter]
    #26212835 - 09/27/19 07:43 AM (4 years, 4 months ago)

Quote:

Edmunter said:
Why do culture sometimes start a little bit away from the transfer.?






It just thickens up, it leaped right from your transfer piece thou, little microscopic threads


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OfflineTweeq
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Re: Cultivation General Discussion [Re: Edmunter]
    #26212912 - 09/27/19 08:34 AM (4 years, 4 months ago)

Quote:

Edmunter said:
Quote:

Tweeq said:
@Edmunter They are 6500K spiral lamps connected to a cheap timer that switches on/off. 12hrs light, 12 hrs darkness




Is it mains or battery?




No batteries. Plugged in the wall socket. Made a little hole in the back plate for the cord


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: stareatclouds]
    #26213047 - 09/27/19 09:21 AM (4 years, 4 months ago)

Quote:

stareatclouds said:
Quote:

verum subsequentis said:
i don't understand why anyone would go through the work of mixing grains. I use WBS.




What work?

Oats have been my primary grain since I started and prefer them. My oats are much cheaper than my WBS, easier to prep, and stretch farther. I mix WBS into grain masters for the smaller inoculation points. I don't like straight WBS/millet as they clump too easily with the pressure I run them at. If I have more WBS leftover, I'll mix them for regular jars because it's all grain.





Yeah, wbs is soo expensive where I live. Like 4x the price of other grains. I prefer oats but if Im feeling energetic I may mix in milo. It helps speed up the colonization when g2g or spawning to buulk.  No more work cause instead of boiling 3 pots of oats i boil 2 pots oats and 1 milo. They all get mixed anyway in the end.  Fill up my coffin sized cooler so I can scoop from it easily.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: TedsDead]
    #26213072 - 09/27/19 09:30 AM (4 years, 4 months ago)

Ed ... Looks like something holding on to me... Culture even looks a bit moldy maybe? There's bacteria at the VERY least... IT'S NOT HEALTHY, let's put it that way...


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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26213097 - 09/27/19 09:43 AM (4 years, 4 months ago)

Quote:

AyePlus said:
Just picked up a 50lb bag of white millet to try out. Can get it locally for 20$ out the door, about 5 more than oats but if I can do away with 3 hour prep time its worth it.



I couldn't be happier with white millet honestly. 12 hour soak of room-temp water (not cold, not hot), 6 hour dry, then load and PC. I put my millet in a bucket with water when I get home night one. The next morning I drain it into a strainer, go to work. I get home and load up the jars and PC for 1h45 min @15psi. It seems like as many steps as oats, but it's such easier steps it's not even funny.

NOTE: The jars come out of the PC looking a little wet in places (usually the bottom 1/2" of the jar). After a bit of dry time they shake up no problem, nothing sticks together any more. Even the grains that burst this way hold their shape and have a "dry" look to them after a day or so.

Running these side-by-side with oats nets me dryer grains when colonized, with less bacterial issues so far. Hard to complain about the $5 extra dollars. And jesus is g2g fast as hell. No joke, g2g->shake at 30% colonized->done is so fast, it's kinda shocking. I wasn't ready for how fast it happens and some of my jars consolidated for way longer than I wanted as a result.

Let me know what you think Aye!

EDIT: side bonus, millet smells kinda delicious when doing g2g. It's like... idk mushroom candy smelling?


Edited by ShaperDreaming (09/27/19 09:45 AM)


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26213135 - 09/27/19 10:07 AM (4 years, 4 months ago)

@star I suppose i was thinking of doing two different preps and mixing. I suppose if the prep is the same and you're just dumping them in together it's not so bad.


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InvisiblePsicomb
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26213145 - 09/27/19 10:11 AM (4 years, 4 months ago)

Quote:

Caps McGee said:
Ed ... Looks like something holding on to me... Culture even looks a bit moldy maybe? There's bacteria at the VERY least... IT'S NOT HEALTHY, let's put it that way...




Agreed, it looks like trouble.  Pretty sure mycelium and something sketchy are all mixed together in there.


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26213149 - 09/27/19 10:12 AM (4 years, 4 months ago)

Yep. I rinse my grain in buckets with paint strainer bags and dump boiling water in and let sit for a few hours. One of the buckets will have WBS. :smile:


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: stareatclouds]
    #26213158 - 09/27/19 10:15 AM (4 years, 4 months ago)

different soak times?


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26213223 - 09/27/19 10:43 AM (4 years, 4 months ago)

Quote:

ShaperDreaming said:
I couldn't be happier with white millet honestly. 12 hour soak of room-temp water (not cold, not hot), 6 hour dry, then load and PC. I put my millet in a bucket with water when I get home night one. The next morning I drain it into a strainer, go to work. I get home and load up the jars and PC for 1h45 min @15psi. It seems like as many steps as oats, but it's such easier steps it's not even funny.




Sonofabitch! I wish I knew about this when I started! This is one more step I can do in my lab, instead of messing up our kitchen with shroom stuff. And doesn't use any gas.


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26213225 - 09/27/19 10:44 AM (4 years, 4 months ago)

The oats get a second dose of boiling water usually. The WBS only needs 45m or so with one, but I'll leave it until I'm ready to deal with it. Nothing tricky.


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Re: Cultivation General Discussion [Re: stareatclouds]
    #26213254 - 09/27/19 10:59 AM (4 years, 4 months ago)

With any grain, soak or boil. I just pop a few in my mouth every so often until theyre how I like and record times.  I used to soak but have been boiling for times sake. The 'brew bags' you can get at a homebrew store are nice for straining and come in 5 and 8 gallon sizes. Homebrew equiptment in general is the shit for mushcult imo


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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OfflineSankhara
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Re: Cultivation General Discussion [Re: TedsDead]
    #26213300 - 09/27/19 11:18 AM (4 years, 4 months ago)

So, i know the norm is to transfer as fast as possible away from contamination.

So my question is, what is the deal with the contamination that is embeded between the mycelium structure? Should be apply the same norm? Or is there a much larger amount of room to transfer in these cases?

I have some petris that i inoculated with a very light print, and the mycelium came out veery transparent, but still with no signs of contamination. After the first trasnfer the muchelium got much more clearer.

I clearly understand there was contamination, but given that the mycelium "has it under control" i feel there should be much more room to allow the mycelium to grow in between transfers. This would allow to pic the mycelium farther away from the center and so much more clean?

It that correct?


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: Sankhara]
    #26213306 - 09/27/19 11:22 AM (4 years, 4 months ago)

You might get lucky sandwhiching the culture in antibiotic agar: but if it's meshed :shrug:

Not always the case to grab immediately:  if I've a satellite bacterial colony near center, and the culture is obviously growing faster, I'll mark the back of the plate under the colony, and allow the mycelium to grow further out from it before transfering... DON'T WAIT WITH OTHER MOLDS...once it sporelates, hang it up and toss it out

Short answer, you CAN get lucky and outrun shit: it's up to you to use your judgement which approach to take in a given situation


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: TedsDead]
    #26213308 - 09/27/19 11:23 AM (4 years, 4 months ago)

Quote:

TedsDead said:
With any grain, soak or boil. I just pop a few in my mouth every so often until theyre how I like and record times.  I used to soak but have been boiling for times sake. The 'brew bags' you can get at a homebrew store are nice for straining and come in 5 and 8 gallon sizes. Homebrew equiptment in general is the shit for mushcult imo




I was about to buy homebrew bags for this, but researched more and went with mesh paint strainer bags. They're way, way cheaper and do the job just as well, if not better. Elastic band at the opening to fit on the bucket securely, durable enough to lift a full bag of wet grain, handles boiling water, etc.


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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: Sankhara]
    #26213323 - 09/27/19 11:31 AM (4 years, 4 months ago)

Quote:

TedsDead said:
Homebrew equiptment in general is the shit for mushcult imo



What other stuff do you use from homebrewing? I've never had an excuse to go into a home brew store and want more! Spill the beans!


Quote:

Sankhara said:
So my question is, what is the deal with the contamination that is embeded between the mycelium structure? Should be apply the same norm? Or is there a much larger amount of room to transfer in these cases?

I have some petris that i inoculated with a very light print, and the mycelium came out veery transparent, but still with no signs of contamination. After the first trasnfer the muchelium got much more clearer.

I clearly understand there was contamination, but given that the mycelium "has it under control" i feel there should be much more room to allow the mycelium to grow in between transfers. This would allow to pic the mycelium farther away from the center and so much more clean?

It that correct?



So... without pictures it's hard to say, however!

This is why we transfer "as little as possible from the leading edge". When I get myc that looks weird in whatever way, I do my damnded to cut tiny tiny slices from the almost transparent leading edge of the myc. Usually the mushrooms are slightly ahead of the contamination, and you can get lucky and unweave them so to speak.

I think I'm kinda confused on your question, because the contamination is also mycelium, just not the myc you're looking for. When it gets enmeshed you can't really tell if you're transferring mushrooms or say trich.

The other problem with what you're talking about is, just because the mushroom has "attacked and covered" say a green spot, doesn't mean that the spores from that green spot aren't floating all around that petri, or enbedded into it, or germinating onto the myc itself (trich loves a good meal of mycelium) :shrug:

Hope this helps answer your question!


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OfflineSankhara
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26213366 - 09/27/19 11:46 AM (4 years, 4 months ago)

Okey? So here is an example of what i mean when i say contaminations embeded in the mycelial structure (mushroom mycelium) there are no clear signs of contamination, not macroscopically at least, i assume because mycelium simply took over.
Still the mycelium was barely able to be seen, and could clearly be confused with a contamination, my guess is that weak growth was because of some contamination. But after the first dΓ±transfer it took a much much healthier look and white color, starting to show more structure as well.

In this case i would not see any reason to hurry transfering for example



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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: Sankhara]
    #26213380 - 09/27/19 11:53 AM (4 years, 4 months ago)

Assuming that I'm seeing condensation on that pic from 20/9, yes, it looks like you have successfully transferred away from your contamination. It is kinda hard to see overall, but yeah, looks pretty good.

I do recommend smaller transfers in the future. Especially when transferring away from contamination, the less you take from the first plate, the better off you are.

Are you asking if it is good to go to grains or something? Personally, if I know I'm transfering from contamination I do at least two transfers to make sure I'm in the clear. A week more and an extra petri dish is much cheaper and quicker than seeing green pop up in a tub.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26213387 - 09/27/19 11:56 AM (4 years, 4 months ago)

Looks awfully whispy, and almost clear near center... I'd keep working it personally... I wouldn't run that culture as is


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Re: Cultivation General Discussion [Re: Sankhara]
    #26213393 - 09/27/19 11:57 AM (4 years, 4 months ago)

Use some food coloring in your PDA. Shit's bone white. Cant tell what im looking at.


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