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OfflineTweeq
Tweeq of Nature
Male

Registered: 06/07/18
Posts: 2,043
Loc: Netherlands Flag
Last seen: 8 hours, 5 minutes
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Re: Cultivation General Discussion [Re: Edmunter]
    #26210999 - 09/26/19 12:59 PM (4 years, 4 months ago)

Quote:

Edmunter said:
So after a conversation that went like this with the wife.......
"Your mushroom shit is taking over the house sort it out'

Me

"princess wants, princess gets'

I have this question

So light is beneficial to all stages of growth but has anyone actually done a back to back light verses dark colonising experiment.  I mean for agar, jars ect.....

Im thinking of building a colonising cupboard and it might have to be in the dark.  If being in the dark makes it colonise slower thats ok but if it cause more problem a light in the chamber/cupboard might have to be introduced.

Help me stay married please!!!!




Something like this?


Ikea cupboard with doors, discretely fitted with heat mats and 6500K spiral lights 12 on / 12 off. Nobody notices and you can put some flowers on top for the wife :smile:


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: Tweeq]
    #26211022 - 09/26/19 01:05 PM (4 years, 4 months ago)

You can absolutely clone a fruit that has sporulated, how else would you confirm that RW was throwing red/brown spores before cloning? Should rince the fruit well to avoid cross contamination with MS... I personally wait until the fruit has grown before picking clones, because you've no idea how quickly the fruit itself will develop, or if it may abort or not... could end up the smallest fruit in the tub!

HEAT MATS!?
:horrified:


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26211043 - 09/26/19 01:15 PM (4 years, 4 months ago)

Quote:

jbgtaa said:
Im not changing anything about any of my op so theres no need to worry about that

My qualm with yalls statements iss...
"you shouldnt see scenescense until 20 transfers" even if im culturing a second generation? say, i fruited MS, cloned, fruited, and cloned again. Does that qualify twenty transfers from MS?

Also, i havent started any grows from spores in a couple of months. Its all been from the same two golden teacher cultures.

Im not saying either of you are wrong at all, just wondering if what you said still applies with those^ conditions.




Instead of viewing it as something quantifiable like transfers, view it as overall stress on the culture. Having children is stressful, but I'd have less gray hairs in my later years if I raised a Cosby kid instead of my punk nephew.

Let's say you swipe spores, notice growth, and transfer to a new plate when the original growth is dime-sized: T1 to T2. When T2 is dime-sized, you take another transfer to be sure for T3.

Now let's say you let the T2 plate envelope the whole dish, sit at room temperature for months, and then take another transfer. Both are T1 to T3, but one has racked up more "stress" (cell divisions) than the other. One has also sat around, had time to acquire nasties (often hidden when a plate is 100%), began drying out, etc.

Every culture is different and there's no fast rule or hard number like "after x transfers, senescence hits" because not all cultures are the same and neither are transfers. In reality, you're dealing with a living organism and each will have different levels of "stress" it can take.

In general, people say don't clone a clone. The reason being, the culture has already been put through plates, colonizing a substrate, and then fruiting. But you can absolutely clone a clone from a tub and still get solid results. And you can find older clone plates pinning and take pin clones with great results, too. Both of those are cloning a clone, but each scenario is a different level of stress on the fruits in question. Just like cloning a pin from a cluster is different than cloning from a mature fruit dropping spores.

Similarly, G2G via pint-to-pint requires less cell divisions than quart-to-quart or bag-to-bag, but you'd likely still mark either these the same with an expansion number.

G2G transfers are much, much more stressful than agar transfers. Agar transfer is a small wedge from 2D surface to another 2D surface. Boom, done.

G2G is:

1. Transfer from agar to grain (usually I do LI, which is agar dropped into jar and blended with blade, so even more stress).

2. Colonizing a new, larger 3D grain substrate with your master jar (stress).

3. Breaking up the mycelium for transfer (stress).

4. Colonizing additional jars (stress).

5. Breaking up the mycelium again for spawning (stress).

6. Colonizing another even larger 3D substrate (stress).

7. Pumping out fruits (stress, baby).

Obviously the G2G scenario is more labor intensive on the myc, but it's important to remember that these "stresses" are normal and myc is very resilient and does whatever it can to keep doing its thing. I only use the term stress to help illustrate the bigger picture of myc health. Not all transfers are equal. Not all grain expansions are equal. Not all examples of clone-to-clone are equal. And these are just the extremely obvious examples of "stress."

If you're paranoid about senescence, slant your proven cultures immediately (baby food jars work great if you can't afford tubes, even though they're cheap AF on Amazon) and fridge them. When you need the young genetics, pull out the slant, take a transfer to a new plate (or a few transfers). When that/those grow out enough to confirm cleanliness, transfer to new plates (however many you want) after one goes to a fresh slant(s) and back in the fridge.


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Offlinepixelpopper
Crap Artist

Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 3 months, 12 days
Re: Cultivation General Discussion [Re: Tweeq]
    #26211049 - 09/26/19 01:18 PM (4 years, 4 months ago)

Quote:

Tweeq said:
Something like this?


Ikea cupboard with doors, discretely fitted with heat mats and 6500K spiral lights 12 on / 12 off. Nobody notices and you can put some flowers on top for the wife :smile:




Nice.. I do something similar with an old stereo cabinet for turntable/records. Has shelves and glass door on front, great for storing colonizing jars while allowing them to receive ambient room light. Decided I am going to start keeping an eye out for more of these cabinets at thrift stores.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
Trusted Cultivator
Re: Cultivation General Discussion [Re: stareatclouds]
    #26211052 - 09/26/19 01:19 PM (4 years, 4 months ago)

Quote:

stareatclouds said:
Quote:

jbgtaa said:
Im not changing anything about any of my op so theres no need to worry about that

My qualm with yalls statements iss...
"you shouldnt see scenescense until 20 transfers" even if im culturing a second generation? say, i fruited MS, cloned, fruited, and cloned again. Does that qualify twenty transfers from MS?

Also, i havent started any grows from spores in a couple of months. Its all been from the same two golden teacher cultures.

Im not saying either of you are wrong at all, just wondering if what you said still applies with those^ conditions.




Instead of viewing it as something quantifiable like transfers, view it as overall stress on the culture. Having children is stressful, but I'd have less gray hairs in my later years if I raised a Cosby kid instead of my punk nephew.

Let's say you swipe spores, notice growth, and transfer to a new plate when the original growth is dime-sized: T1 to T2. When T2 is dime-sized, you take another transfer to be sure for T3.

Now let's say you let the T2 plate envelope the whole dish, sit at room temperature for months, and then take another transfer. Both are T1 to T3, but one has racked up more "stress" (cell divisions) than the other. One has also sat around, had time to acquire nasties (often hidden when a plate is 100%), began drying out, etc.

Every culture is different and there's no fast rule or hard number like "after x transfers, senescence hits" because not all cultures are the same and neither are transfers. In reality, you're dealing with a living organism and each will have different levels of "stress" it can take.

In general, people say don't clone a clone. The reason being, the culture has already been put through plates, colonizing a substrate, and then fruiting. But you can absolutely clone a clone from a tub and still get solid results. And you can find older clone plates pinning and take pin clones with great results, too. Both of those are cloning a clone, but each scenario is a different level of stress on the fruits in question. Just like cloning a pin from a cluster is different than cloning from a mature fruit dropping spores.

Similarly, G2G via pint-to-pint requires less cell divisions than quart-to-quart or bag-to-bag, but you'd likely still mark either these the same with an expansion number.

G2G transfers are much, much more stressful than agar transfers. Agar transfer is a small wedge from 2D surface to another 2D surface. Boom, done.

G2G is:

1. Transfer from agar to grain (usually I do LI, which is agar dropped into jar and blended with blade, so even more stress).

2. Colonizing a new, larger 3D grain substrate with your master jar (stress).

3. Breaking up the mycelium for transfer (stress).

4. Colonizing additional jars (stress).

5. Breaking up the mycelium again for spawning (stress).

6. Colonizing another even larger 3D substrate (stress).

7. Pumping out fruits (stress, baby).

Obviously the G2G scenario is more labor intensive on the myc, but it's important to remember that these "stresses" are normal and myc is very resilient and does whatever it can to keep doing its thing. I only use the term stress to help illustrate the bigger picture of myc health. Not all transfers are equal. Not all grain expansions are equal. Not all examples of clone-to-clone are equal. And these are just the extremely obvious examples of "stress."

If you're paranoid about senescence, slant your proven cultures immediately (baby food jars work great if you can't afford tubes, even though they're cheap AF on Amazon) and fridge them. When you need the young genetics, pull out the slant, take a transfer to a new plate (or a few transfers). When that/those grow out enough to confirm cleanliness, transfer to new plates (however many you want) after one goes to a fresh slant(s) and back in the fridge.



Should screenshot this and ask u to sign my book:lol:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: cronicr] * 1
    #26211165 - 09/26/19 02:09 PM (4 years, 4 months ago)

I thought you already released your book?



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OfflinecronicrFacebook
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Posts: 61,436
Loc: Van Isle Flag
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Re: Cultivation General Discussion [Re: stareatclouds] * 1
    #26211178 - 09/26/19 02:11 PM (4 years, 4 months ago)

:lol: nice to see ya stare


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: stareatclouds]
    #26211189 - 09/26/19 02:14 PM (4 years, 4 months ago)

Mmm...gotta get those vitamins


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InvisibleJust_A_Noob
Breathing
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: Cultivation General Discussion [Re: cronicr]
    #26211190 - 09/26/19 02:14 PM (4 years, 4 months ago)

Send a copy to Trypt.  I’ll pay for shipping


--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links


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InvisibleTedsDead
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Registered: 01/03/17
Posts: 4,998
Trusted Cultivator
Re: Cultivation General Discussion [Re: cronicr]
    #26211199 - 09/26/19 02:18 PM (4 years, 4 months ago)

:takingnotes: not ass eating notes


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house Flag
Last seen: 3 hours, 4 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: TedsDead]
    #26211242 - 09/26/19 02:38 PM (4 years, 4 months ago)

Ass eating for dummies was way better


--------------------

Crack's Tek's and Shit



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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Crackatoa]
    #26211253 - 09/26/19 02:42 PM (4 years, 4 months ago)

Keep in mind, not all asses you eat are created equal.


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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Cultivation General Discussion [Re: stareatclouds]
    #26211293 - 09/26/19 03:02 PM (4 years, 4 months ago)

jbgtaa/badsponge: I should state that oats are fine as a grain that I've had great success with at times. The reasons I'm moving away from oats has to do with what's been going on with my oats in my location. I have two feed stores that are within an hour of me. The next closest is 1h45m away. I have to make due with what I have available, you know?

I also tried to make Rye work, which ... I don't even understand how my results were so far off from what other people's were using their exact same teks. I tried 4 teks, all bad results, wasted over 20# of the stuff. Millet was great the first time, better the second :shrug: I also have some milo to test, and may mix those two together.

Tweeq/Edmunster: Glad you two met. I was absolutely going to recommend your space as a footprint. My first year of growing was all done in about a 3'x4' space in a closet. Run lights into it, I promise you'll be in good shape :heart:


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,399
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26211301 - 09/26/19 03:06 PM (4 years, 4 months ago)

I'm thinking about mixing oats and WBS(mostly Millet) and seeing how that works. Next time I need to make grain I'm gonna try that shit out.


--------------------

Crack's Tek's and Shit



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OfflineEdmunter
Mr
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Registered: 05/01/13
Posts: 5,699
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Re: Cultivation General Discussion [Re: Tweeq]
    #26211323 - 09/26/19 03:13 PM (4 years, 4 months ago)

Quote:

Tweeq said:
Quote:

Edmunter said:
So after a conversation that went like this with the wife.......
"Your mushroom shit is taking over the house sort it out'

Me

"princess wants, princess gets'

I have this question

So light is beneficial to all stages of growth but has anyone actually done a back to back light verses dark colonising experiment.  I mean for agar, jars ect.....

Im thinking of building a colonising cupboard and it might have to be in the dark.  If being in the dark makes it colonise slower thats ok but if it cause more problem a light in the chamber/cupboard might have to be introduced.

Help me stay married please!!!!




Something like this?


Ikea cupboard with doors, discretely fitted with heat mats and 6500K spiral lights 12 on / 12 off. Nobody notices and you can put some flowers on top for the wife :smile:




Nice

Whats the lighting set up?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Crackatoa]
    #26211332 - 09/26/19 03:15 PM (4 years, 4 months ago)

Nice Sharper, getting us back on point here. Was about to bust out with my ass eating wisdom.

Anyway, Von SAB:


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: Feasoghorm]
    #26211350 - 09/26/19 03:22 PM (4 years, 4 months ago)

id want bigger holes:naughty:


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: stareatclouds]
    #26211371 - 09/26/19 03:29 PM (4 years, 4 months ago)

Quote:

Crackatoa said:
I'm thinking about mixing oats and WBS(mostly Millet) and seeing how that works. Next time I need to make grain I'm gonna try that shit out.




This is what I do for most of my jars, but especially grain masters. I sift my WBS through a bucket I drilled a bunch of holes into and wind up separating the sunflowers and cracked corn. Then I mix the hydrated oats and WBS at roughly 1.6:1 ratio in favor of the WBS.


Quote:

stareatclouds said:
Do you have any metrics on how much weight they put on with your soak method? I just dump boiling water in a Home Depot bucket, toss the WBS + gypsum in and leave it for a few hours. I usually notice a 40% weight increase when all is said and done (fan dried, ready to be loaded into jars/bags). For comparison, my oats see an average 85% increase, but I run those buckets with a second batch of boiling water after 1-3 hours in the first.

It's probably also worth noting that I strain the corn/sunflower seeds from my WBS so I only wind up with milo and millet.



Look at me go!






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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: mushboy]
    #26211386 - 09/26/19 03:34 PM (4 years, 4 months ago)

Quote:

mushboy said:
id want bigger holes:naughty:



:eatingout::eatingout::eatingout:

Oh wait, are you being sarcastic?


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: Feasoghorm]
    #26211410 - 09/26/19 03:39 PM (4 years, 4 months ago)

Quote:

GypsyCurse said:





they are slightly small and kinda far apart:shrug:

whatever works for ya. my first sab look like sloth from goonies


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Shop: Bridgetown Botanicals CBD Concentrates   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Kratom Capsules for Sale   North Spore Bulk Substrate


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