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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Edmunter]
    #26208816 - 09/25/19 02:53 PM (4 years, 4 months ago)

I'd recommend cooking them up separately.
You could noc a grain jar with a cake but it'd have to be done correctly. In a SAB or flow you could dig a little out with a sterilized spoon and put it in the grain jar.


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26208831 - 09/25/19 03:02 PM (4 years, 4 months ago)

Ive heard of people using BRF cakes as masters, although its probably easier to just use smaller grain jars. I personally just use 1 quarts jar to 10 quart jars with no problem and fast colonization.

Some people here are saying to do them separately and i can see why, but i personally do not. Lately i havent had grain jars sit un-inoculated for longer than 4 days, but before that they would sit for at least a week. I think a period of wait is good for them, as you will be able to tell if u sterilized properly. Like i said, though, the last 5 cases of jars ive made have been used within a day of sterilization as receiving jars. I dont really think that it truly matters, as long as your filter is solid and it's not in a disgustingly dirty area.

If i were you, I'd make up a whole case of grain jars and inoculate 4 of them, since at least 1 and probably more are likely to fail, and leave the others set to verify that your sterilization is on point. If it is on point, you can use them when your master is done colonizing.

Also if i were you, i wouldnt expand a multispore syringe jar. Just inoculate one jar and fruit it to get clones. By that time you should have some practice with agar right? I personally dont see any reason to expand a jar that has raw spores in it, as it's just another chance to contaminate. Fruit the multispore jar by itself, and by that time youll be proficient enough in agar to take a clone, and then you're really working.

Good luck, and welcome!!!


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26208840 - 09/25/19 03:08 PM (4 years, 4 months ago)

All valid points. I just like to grow more mushrooms so I use up all the jars and make up a whole new batch next time.


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26208906 - 09/25/19 03:34 PM (4 years, 4 months ago)

Quote:

verum subsequentis said:
All valid points. I just like to grow more mushrooms so I use up all the jars and make up a whole new batch next time.




Thats what ive been doing for the past 2 months so i know where youre at and i dont let jars sit around at all anymore simply for the fact that i literally prep 1 case per master quart, so i just prep a day before my g2g.


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OfflineMeysterZ
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26208917 - 09/25/19 03:38 PM (4 years, 4 months ago)

I have only one PF cake at the moment, which is why i want to make some grain spawn to increase my total spawn for bulking later (shoeboxes)

Also one more thing, i am a bit confused about "fruiting conditions" when using a shoebox or a monotub. Should the tubs initially be covered with something or kept in dark until they colonize? also since both the shoebox and monotubs already have some FAE, what is different about fruiting conditions? more FAE by taking the lid off or?

Also what is the purpose of putting a plastic/grocery bag on top of a shoebox instead of a lid?

Sorry for this bunch of questions, i searched around but this is still not clear to me, if someone  can point out or provide some links to  write ups explaining this stuff i would appreciate it a lot :smile:

Quote:

jbgtaa said:


If i were you, I'd make up a whole case of grain jars and inoculate 4 of them, since at least 1 and probably more are likely to fail, and leave the others set to verify that your sterilization is on point. If it is on point, you can use them when your master is done colonizing.






That's a great idea which i thought about also and its probably what i will end up doing.

Quote:

jbgtaa said:

Also if i were you, i wouldnt expand a multispore syringe jar. Just inoculate one jar and fruit it to get clones. By that time you should have some practice with agar right? I personally dont see any reason to expand a jar that has raw spores in it, as it's just another chance to contaminate. Fruit the multispore jar by itself, and by that time youll be proficient enough in agar to take a clone, and then you're really working.






If i am understanding this correctly, the issue with MS syringe is that the results are random, but if i make a clone on agar i can  then isolate & transfer  good strains and then use them later  for better results? or am i completely mixing  this up? :confused:


Also, Thanks everyone for the replies


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: MeysterZ]
    #26208928 - 09/25/19 03:42 PM (4 years, 4 months ago)

Quote:

MeysterZ said:
If i am understanding this correctly, the issue with MS syringe is that the results are random, but if i make a clone on agar i can  then isolate & transfer  good strains and then use them later  for better results?




You got it!


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InvisibleFeasoghorm

Registered: 10/24/18
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Re: Cultivation General Discussion [Re: jbgtaa]
    #26208936 - 09/25/19 03:45 PM (4 years, 4 months ago)

Dang 1 to 10... I do 1qt to 4qts g2g. I need shit to happen in a week.

Still having plate contam madness over here. Went from 4% contamination rate to like 15 or 20%. That's fucked. Mostly differnt contams now. I still get a few little bubbles of bacteria i can jst cut out but i been getting a lot of some other fungus that is ravenous and invisible at first unless you shine a torch strait at the surface to get the light reflecting of it. Looks like clear spider web. Bullshit. Taking over.

Keeping anything in the dark is kinda outdated. You do not need to restrict fae during substrate colonization.
As for shoeboxes, grocery bags jst work well.


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OfflineMeysterZ
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Re: Cultivation General Discussion [Re: badsponge]
    #26208946 - 09/25/19 03:48 PM (4 years, 4 months ago)

Quote:

badsponge said:


I started with Bod's tek, but I thought it over-hydrated my oats. I switched to Eat's method 1: https://www.shroomery.org/forums/showflat.php/Number/22158748.

Between boiling and sterilizing. you should wait no more than 24 hrs (per Bod). After you sterilize, you can store jars for a few weeks.




Thanks for pointing out the Tek, i'm checking it out now.

Btw how can you get your oats over hydrated? can't you just leave them longer on the strainer to dry out before putting them in a jar?


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: MeysterZ]
    #26208957 - 09/25/19 03:53 PM (4 years, 4 months ago)

No, the inside can get over-hydrated, and would take forever to dry out. When I used Bod's tek, I'd let my grains dry overnight, PC them, and inside the jars were really wet, even a few days after PC. I use Eat's method now, they're much drier.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: MeysterZ]
    #26208965 - 09/25/19 03:58 PM (4 years, 4 months ago)

No. If theyre over hydrated it means you burst too many kernals overcooking them and they will likely clump once sterilized.

Not sure about your oats but aside from the drying part mine literally have bods oat tek written on the side as cooking instructions with alittle diagram too


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: TedsDead]
    #26208992 - 09/25/19 04:13 PM (4 years, 4 months ago)

Quote:

TedsDead said:
No. If theyre over hydrated it means you burst too many kernals overcooking them and they will likely clump once sterilized.

Not sure about your oats but aside from the drying part mine literally have bods oat tek written on the side as cooking instructions with alittle diagram too




Oh, ok. I don't know why, but I seem to be having better luck with Eat's tek.  But I'm giving up on oats, anyway. I'm gonna burn through my current stock, then I'm switching to millet.


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26209059 - 09/25/19 04:39 PM (4 years, 4 months ago)

Quote:

GypsyCurse said:
Dang 1 to 10... I do 1qt to 4qts g2g. I need shit to happen in a week.

Still having plate contam madness over here. Went from 4% contamination rate to like 15 or 20%. That's fucked. Mostly differnt contams now. I still get a few little bubbles of bacteria i can jst cut out but i been getting a lot of some other fungus that is ravenous and invisible at first unless you shine a torch strait at the surface to get the light reflecting of it. Looks like clear spider web. Bullshit. Taking over.

Keeping anything in the dark is kinda outdated. You do not need to restrict fae during substrate colonization.
As for shoeboxes, grocery bags jst work well.



Why are you cutting out the contams? You're supposed to transfer the leading edge to
a new plate. Your contam rate is probably high cause you're not cleaning up any cultures,
just letting them stew in the originally contaminated plate


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: natedawgnow]
    #26209090 - 09/25/19 04:56 PM (4 years, 4 months ago)

:horrified:


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InvisibleFeasoghorm

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Re: Cultivation General Discussion [Re: natedawgnow]
    #26209091 - 09/25/19 04:57 PM (4 years, 4 months ago)

If a plate i need that is freshly transfered to and has a bubble of bac away from the myc, imma cut it out. Anymore than that and i take a leading edge transfer to a new plate, that is hopefully clean.

Im also not innoculating grain anymore without at least a T.3 plate that i have watched grow every day of it's god damn life.

Im hoping that putting a bandito bandana around my muzzle will cut down on air gusts that happen when i breathe. I see absolutly no other new way that all this vexation cud climb abord.
I shut down ALL air current in my room before i even wipe the inside of my sab.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26209103 - 09/25/19 05:02 PM (4 years, 4 months ago)

Flame your scalpel longer, and wipe it down with iso when you wipe down your hands, wipe down your plates before you open them., the cultivator is usually the source of bacterial contamination. Most of those are a tad overkill tbh but might help.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: AyePlus]
    #26209113 - 09/25/19 05:06 PM (4 years, 4 months ago)

Be sure and flame the center of the blade out, or only use the tip as well: I noticed a dramatic decrease in satellite bacterial colonies when I started being more mindful about more complete flaming, and using "just the tip"


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Offlinepixelpopper
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Re: Cultivation General Discussion [Re: AyePlus]
    #26209119 - 09/25/19 05:08 PM (4 years, 4 months ago)

Quote:

AyePlus said:
Flame your scalpel longer, and wipe it down with iso when you wipe down your hands




For the sake of clarity, be sure to only wipe your scalpel prior to flaming, not after

Sometimes I'll flame down into the handle of the scalpel as well just to be a little extra careful


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InvisibleFeasoghorm

Registered: 10/24/18
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Re: Cultivation General Discussion [Re: pixelpopper]
    #26209195 - 09/25/19 05:44 PM (4 years, 4 months ago)

I torch the blade till it glows and about an inch of the handle back from the blade. Lotta times the handle is too hot to work with. Gotta order an actual scalpel 2mrrw. This exacto is bullshit. Not bad for lancing roids though. I thnk the screw dealy that holds the blade in is chucking shit on my plates even after i burn it.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26209359 - 09/25/19 06:55 PM (4 years, 4 months ago)

No need to flame that far just what's going in, be careful to only place as much of yourself or your instruments over media as needed to do work


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26209420 - 09/25/19 07:20 PM (4 years, 4 months ago)

Lol. I never knew it cause of my callused hands but my gf was complainit about not being able to make transfers cause the xacto handle would get too hot before she properly flamed it.  A propper scalpel  fixed this


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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