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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26206339 - 09/24/19 11:34 AM (4 years, 4 months ago) |
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I seemed to have prepped this grain dry.. Hopefully these dont stall on me, but they probably will. I have plenty of patience, not trying to rush anything just trying to make sure i get an idea of my fuckups, for the longest time i just threw things together and waited. I need to document dates more so im not guessing anymore
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26206351 - 09/24/19 11:38 AM (4 years, 4 months ago) |
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Thank you! I have streaked about 3 plates. Petris are getting out of economical reach, will try to see if i can find any pp5 to make pasty plastes and streak them!
If not i will have to go the grain route to see if i get any lucky and then transfer to some petris to save money.
Saw your link verum! It was quite an interesting post by the way!
-------------------- How would you rate the quality of my answer?
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
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Re: Cultivation General Discussion [Re: natedawgnow]
#26206361 - 09/24/19 11:46 AM (4 years, 4 months ago) |
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Quote:
natedawgnow said:
Quote:
Crackatoa said: Do PE6 look like this, I thought PE6 was a normal looking cube. But these look like my APE pins.

That's called hitting the pe6 genetic lottery 
Maybe 10% of pe6 grows from spore have pe characteristics from what Ive seen. I've grown it a bunch but never got any pe looking fruits sadly
Not saying they won't end up pe-like. But with the runs i do/did from this same lineage tend to get nice large thick fruits, but no notable PE looking fruits. Potency is solid and reliable, but again not that 2x PE potency.
Not the best pic, but it's a 30-something quart tub with surface area bit larger than a shoebox.

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Cthulhu23
Elder God Extraordinaire



Registered: 07/04/18
Posts: 620
Last seen: 2 years, 6 months
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Quote:
verum subsequentis said: putting a grain to agar is a good way to go but i'd more highly recommend streaking four or five plates. I'd skip the sterile glass step though. Just shake the hell out of the syringe to distribute the spores, flame the needle and loop, put a drip on the loop and streak the plate.
I took some pictures of myself doing this with an LC. Maybe they'll help. It's a ways down in the op.
I’ve had a similar “hard to inoculate” syringe of CRS that’s been resisting my agar on multiple plates. Gonna pour some with different nutes (brf) tonight and see how that works - but I hadn’t even thought to try directly to grain (shows how conditioned I am!).
Thanks for the tip!
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: natedawgnow]
#26206458 - 09/24/19 12:30 PM (4 years, 4 months ago) |
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Quote:
natedawgnow said:
Quote:
Crackatoa said: Do PE6 look like this, I thought PE6 was a normal looking cube. But these look like my APE pins.

That's called hitting the pe6 genetic lottery 
Maybe 10% of pe6 grows from spore have pe characteristics from what Ive seen. I've grown it a bunch but never got any pe looking fruits sadly
How strong are they in comparison? to both
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
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Re: Cultivation General Discussion [Re: Edmunter]
#26208326 - 09/25/19 11:08 AM (4 years, 4 months ago) |
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So any input on if I am just wasting my time. I see people burying bad spawn and old cakes outside and getting some mushrooms to pop up. I had few quarts I never spawned that looked pretty bacterial, so I took them to that hill, kinda "dug" away a hole and dumped the grain, then just covered with mulch, leaves, hay and other random debris over there.
I have been trying to keep it damp a few times a day.
No expectations, as this was just the alternative to going in the dumpster
but seeing as I didn't even really use soil or anything, you think this is just wasted effort, minimal as it was?
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: Camera93]
#26208341 - 09/25/19 11:15 AM (4 years, 4 months ago) |
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Quote:
Camera93 said:

So any input on if I am just wasting my time. I see people burying bad spawn and old cakes outside and getting some mushrooms to pop up. I had few quarts I never spawned that looked pretty bacterial, so I took them to that hill, kinda "dug" away a hole and dumped the grain, then just covered with mulch, leaves, hay and other random debris over there.
I have been trying to keep it damp a few times a day.
No expectations, as this was just the alternative to going in the dumpster
but seeing as I didn't even really use soil or anything, you think this is just wasted effort, minimal as it was?
Unless this is early morning or late evening sun that isnt shinning on that all day its the wrong position.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: Camera93]
#26208346 - 09/25/19 11:17 AM (4 years, 4 months ago) |
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Its all about climate. Around here it's either too dry or too cold, theres never a time when it's humid enough and warm enough.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 9 hours, 11 minutes
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26208366 - 09/25/19 11:30 AM (4 years, 4 months ago) |
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Ahh, well damn hopefully something eats the grain at least then
Pic was taken at like 1230pm here in northern ohio
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: Camera93]
#26208379 - 09/25/19 11:43 AM (4 years, 4 months ago) |
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Midday sun and mushrooms never mix
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: Camera93]
#26208380 - 09/25/19 11:43 AM (4 years, 4 months ago) |
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Yea, its pretty damn dry over here in the west. I doubt you could manage an outdoor grow, but with enough messing around you could figure something out, itll just take a lot of work
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: Camera93]
#26208440 - 09/25/19 12:13 PM (4 years, 4 months ago) |
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Quote:
Camera93 said:

So any input on if I am just wasting my time. I see people burying bad spawn and old cakes outside and getting some mushrooms to pop up. I had few quarts I never spawned that looked pretty bacterial, so I took them to that hill, kinda "dug" away a hole and dumped the grain, then just covered with mulch, leaves, hay and other random debris over there.
I have been trying to keep it damp a few times a day.
No expectations, as this was just the alternative to going in the dumpster
but seeing as I didn't even really use soil or anything, you think this is just wasted effort, minimal as it was?
What I do is throw my spent spawn in my vegetable garden the veggies provide plenty of shade and they get water atleast once a day. I'd suggest either 1. Picking another spot with more shade 2. Transplanting some nice plants to the area to provide shade. Or 3. Make something to provide shade and still allow good air flow. I'd suggest if you have a 5 gallon bucket laying around cut the bottom out and just slap that around your spawn pile. You could alternatively make the bucket into a sgfc design with 1/4" holes in a grid pattern. Both would be easier than building a small canopy/humidity tent. Where I am the climate is horrible for cubes but I still manage to get outdoor crop all summer long. Just make your own conditions.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: gizmo1]
#26208461 - 09/25/19 12:25 PM (4 years, 4 months ago) |
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Quote:
gizmo1 said:
Quote:
Camera93 said:

So any input on if I am just wasting my time. I see people burying bad spawn and old cakes outside and getting some mushrooms to pop up. I had few quarts I never spawned that looked pretty bacterial, so I took them to that hill, kinda "dug" away a hole and dumped the grain, then just covered with mulch, leaves, hay and other random debris over there.
I have been trying to keep it damp a few times a day.
No expectations, as this was just the alternative to going in the dumpster
but seeing as I didn't even really use soil or anything, you think this is just wasted effort, minimal as it was?
What I do is throw my spent spawn in my vegetable garden the veggies provide plenty of shade and they get water atleast once a day. I'd suggest either 1. Picking another spot with more shade 2. Transplanting some nice plants to the area to provide shade. Or 3. Make something to provide shade and still allow good air flow. I'd suggest if you have a 5 gallon bucket laying around cut the bottom out and just slap that around your spawn pile. You could alternatively make the bucket into a sgfc design with 1/4" holes in a grid pattern. Both would be easier than building a small canopy/humidity tent. Where I am the climate is horrible for cubes but I still manage to get outdoor crop all summer long. Just make your own conditions.
Do you throw contaminated spawn in too?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: gizmo1]
#26208464 - 09/25/19 12:26 PM (4 years, 4 months ago) |
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Shade cloth is an excellent option that I highly recommend for outdoor patches that lack vegetation and shade. It can be ordered to any size from Amazon. 50% shade cloth should suffice, I've gotten away with using 40% but probably would stick to 50+ depending on your geography and species.
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26208523 - 09/25/19 12:51 PM (4 years, 4 months ago) |
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Love the feedback, thank you all!
I have another question bugging me If you have green pop up on a petri dish, do you try and transfer away from it? Or is the fact that it sporulated inside the plate kinda mean the whole culture is now fucked? I have a pastyplate that got a green spot on one end, looks like clean...er myc on the other and I want to try and clean it up..but worry ill just take the mold with me to the next plate
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: Camera93]
#26208541 - 09/25/19 12:55 PM (4 years, 4 months ago) |
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Try transferring away the clean myc. If it doesn't work, you lose a couple plates, no big deal.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: badsponge]
#26208685 - 09/25/19 01:49 PM (4 years, 4 months ago) |
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Transfer away and then the very first growth you get from the next plate transfer away again.
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MeysterZ
Work in progress


Registered: 09/11/19
Posts: 11
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: badsponge]
#26208767 - 09/25/19 02:26 PM (4 years, 4 months ago) |
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Hello Everyone, I am newbie here and i just recently started my first grow, i've been reading a ton lately and i'm very thankful for all the write ups and comments you guys are putting out, you really are helping us new guys a lot.
However some things are still not clear to me so i wanted to ask some questions here instead of making a new thread.
1) I wanted to make some grain spawn so i decided to use Bod's oat spawn Tek, but i"m not sure should i cook & PC all the grain for the master jar and receiving jars at the same time, because i"m not sure how long can those jars stay w/out being inoculated, as it would surely take some time for the nocc'd jar to colonize and be ready for g2g. What do you usually do in this situation, do you prep it all together or do it separately for the main jar and receiving jars?
2) Is it possible to use a PF Jar to inoculate a grain jar? I wanted to use it instead of a spore syringe as i suspect the syringe might be contaminated, and i'm still waiting for my petri dishes to arrive so i can't use agar atm. The main thing i'm concerned about is what will happen once i remove the dry verm layer at the top, because some contaminants that the layer stopped(which are maybe sitting now at the top) now might get in touch with the cake inside.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: MeysterZ]
#26208796 - 09/25/19 02:43 PM (4 years, 4 months ago) |
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Quote:
MeysterZ said: Hello Everyone, I am newbie here and i just recently started my first grow, i've been reading a ton lately and i'm very thankful for all the write ups and comments you guys are putting out, you really are helping us new guys a lot.
However some things are still not clear to me so i wanted to ask some questions here instead of making a new thread.
1) I wanted to make some grain spawn so i decided to use Bod's oat spawn Tek, but i"m not sure should i cook & PC all the grain for the master jar and receiving jars at the same time, because i"m not sure how long can those jars stay w/out being inoculated, as it would surely take some time for the nocc'd jar to colonize and be ready for g2g. What do you usually do in this situation, do you prep it all together or do it separately for the main jar and receiving jars?
2) Is it possible to use a PF Jar to inoculate a grain jar? I wanted to use it instead of a spore syringe as i suspect the syringe might be contaminated, and i'm still waiting for my petri dishes to arrive so i can't use agar atm. The main thing i'm concerned about is what will happen once i remove the dry verm layer at the top, because some contaminants that the layer stopped(which are maybe sitting now at the top) now might get in touch with the cake inside.
How many cakes do you have? If you have enough you can spawn that to bulk.
When I make masters I make 2-4 (Maybe 2 plates/cultures split in 2) at a time and would never leave jars waiting on the side for that to colonise as I could grow something else in that time.
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: MeysterZ]
#26208815 - 09/25/19 02:53 PM (4 years, 4 months ago) |
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Quote:
MeysterZ said: I wanted to make some grain spawn so i decided to use Bod's oat spawn Tek, but i"m not sure should i cook & PC all the grain for the master jar and receiving jars at the same time, because i"m not sure how long can those jars stay w/out being inoculated, as it would surely take some time for the nocc'd jar to colonize and be ready for g2g. What do you usually do in this situation, do you prep it all together or do it separately for the main jar and receiving jars?
I started with Bod's tek, but I thought it over-hydrated my oats. I switched to Eat's method 1: https://www.shroomery.org/forums/showflat.php/Number/22158748.
Between boiling and sterilizing. you should wait no more than 24 hrs (per Bod). After you sterilize, you can store jars for a few weeks.
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