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OfflineEdmunter
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Re: Is it possible to breed genetics? [Re: verum subsequentis]
    #26195746 - 09/18/19 12:55 PM (4 years, 4 months ago)

What would you say your transfer to fully grown plate time scale is?  Mine for some reason have really slowed.  I might try grain water for a bit.


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OfflineSankhara
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Re: Cultivation General Discussion [Re: Inocuole]
    #26195747 - 09/18/19 12:55 PM (4 years, 4 months ago)

Well i have a question... A very noob question but, what the hell, i didnt find almost anything about it.

How does mycelium look to you when its germinating on agar?

I streaked some plates last week and didnt see any growth until two days ago. When only while looking from the sides on a very well lighted room i could simply see very transparent mycelium like structures growing, now they are beginning to show some hairy growth on the top.

Does this happen to you? Or does mycelium always puf up from the very first signs of  growth and then grow out?

Hope i am explaining myself well... Cant take a picture because its kind of transparent you know... :wink:
I know time will tell, but i am just curious!


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OfflineCamera93
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Re: Cultivation General Discussion [Re: Sankhara]
    #26195750 - 09/18/19 12:57 PM (4 years, 4 months ago)

Quote:

Sankhara said:
Well i have a question... A very noob question but, what the hell, i didnt find almost anything about it.

How does mycelium look to you when its germinating on agar?

I streaked some plates last week and didnt see any growth until two days ago. When only while looking from the sides on a very well lighted room i could simply see very transparent mycelium like structures growing, now they are beginning to show some hairy growth on the top.

Does this happen to you? Or does mycelium always puf up from the very first signs of  growth and then grow out?

Hope i am explaining myself well... Cant take a picture because its kind of transparent you know... :wink:
I know time will tell, but i am just curious!






Germ Plates


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineSankhara
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Re: Cultivation General Discussion [Re: Camera93]
    #26195756 - 09/18/19 01:02 PM (4 years, 4 months ago)

I believe im on the right path then. Im using rice agar, so that may affect the way it can be seen. Still i find it weird how growth is developing... Not how i remembered from my last experiences, but still i have now doubt is mycelium

Thank you for the examples!! Your plates look incredibly transparent also, thats incredible, you poured your agar really thin right?


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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Sankhara]
    #26195758 - 09/18/19 01:03 PM (4 years, 4 months ago)

Quote:

Sankhara said:
Well i have a question... A very noob question but, what the hell, i didnt find almost anything about it.

How does mycelium look to you when its germinating on agar?

I streaked some plates last week and didnt see any growth until two days ago. When only while looking from the sides on a very well lighted room i could simply see very transparent mycelium like structures growing, now they are beginning to show some hairy growth on the top.

Does this happen to you? Or does mycelium always puf up from the very first signs of  growth and then grow out?

Hope i am explaining myself well... Cant take a picture because its kind of transparent you know... :wink:
I know time will tell, but i am just curious!



My germ plates always look weird as hell. The thing about germ plates is, theyre usually contaminated a small degree, and its hard to determine what is what is the very first moments of mycelial life. Sometimes i have a streak plate that looks like shit, and sometimes i have each inoc point halo out. Germ plates are the most unreliable of cultures we as mycologists have, that's why we take multipe transfers away from it to make sure we have a clean culture.

Try using clear MEA/PDA for your germ plates, low nute so that is both transparent, for differentiating between mycelium and bacteria, and supposedly low nutes bring out rhizo growth, so that way you can find a good sector.


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If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Sankhara]
    #26195761 - 09/18/19 01:05 PM (4 years, 4 months ago)

Not sure about the time scale. I don't keep that close of track on my plates. I usually have a whole pile of plates that I'm working on and just check on them from time to time. It's roughly a week to a week and a half but it entirely depends on the culture. I don't trip on letting them grow a certain amount though. I just use them up whenever their are enough that need worked on. Sometimes this involves plates at every stage of growth.

Sankhara, Without pictures i can't tell you exactly what I think but it is true that freshly germed spores can produce translucent myc. If you really want to nerd out you can start studying Monokaryotic and dikaryotic mycelium, anastomosis and all of the more advanced aspects of what we do. Also, you can order a nice scope and really nerd out BUT, it's not necessary.

Best answer, without pictures, is this: Take a few tiny transfers to new plates. Take from the leading edge of the fastest growth available.


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OfflineSankhara
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26195771 - 09/18/19 01:11 PM (4 years, 4 months ago)

Thanks verum! will be reading up a bit on those topics for sure! This mycology shit is really interesting for sure.

jbgtaa i believe you may be right, i bought a very clear print that happened to be on sale, so probably contamiantion may be playing its role. Once it shows a more pronounced and healthy grow i will transfer until it looks nice. Thanks for the advice aswell!

:sun:


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OfflineCamera93
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Re: Cultivation General Discussion [Re: Sankhara]
    #26195774 - 09/18/19 01:11 PM (4 years, 4 months ago)

Quote:

Sankhara said:
Thank you for the examples!! Your plates look incredibly transparent also, thats incredible, you poured your agar really thin right?




These were PP5 deli cup container (more on that) and I used grain soak water for the nutes.
Yes I poured relatively thin, enough to cover the bottom and rise above the little inner lip in the center.


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinejbgtaa
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Re: Cultivation General Discussion [Re: Sankhara]
    #26195777 - 09/18/19 01:15 PM (4 years, 4 months ago)

Quote:

Sankhara said:
Thanks verum! will be reading up a bit on those topics for sure! This mycology shit is really interesting for sure.

jbgtaa i believe you may be right, i bought a very clear print that happened to be on sale, so probably contamiantion may be playing its role. Once it shows a more pronounced and healthy grow i will transfer until it looks nice. Thanks for the advice aswell!

:sun:



I was more saying that germed myc can LOOK like contam, not that its present on your plate. You should really post a pic like verum said, so we can see. 
I was also saying at the same time that germ plates almost always contam, because spores arent clean. Sometimes you just gotta let em play out.


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If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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OfflineEdmunter
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26195782 - 09/18/19 01:19 PM (4 years, 4 months ago)

Quote:

verum subsequentis said:
Not sure about the time scale. I don't keep that close of track on my plates. I usually have a whole pile of plates that I'm working on and just check on them from time to time. It's roughly a week to a week and a half but it entirely depends on the culture. I don't trip on letting them grow a certain amount though. I just use them up whenever their are enough that need worked on. Sometimes this involves plates at every stage of growth.

Sankhara, Without pictures i can't tell you exactly what I think but it is true that freshly germed spores can produce translucent myc. If you really want to nerd out you can start studying Monokaryotic and dikaryotic mycelium, anastomosis and all of the more advanced aspects of what we do. Also, you can order a nice scope and really nerd out BUT, it's not necessary.

Best answer, without pictures, is this: Take a few tiny transfers to new plates. Take from the leading edge of the fastest growth available.




Im totally with you.  I need more structure in my work as one minute its all going fine and the next thing I have 50 plates working on 5 different projects and my head hurts.


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Edmunter]
    #26195791 - 09/18/19 01:24 PM (4 years, 4 months ago)

i know the feeling very well


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: verum subsequentis]
    #26195826 - 09/18/19 01:39 PM (4 years, 4 months ago)

I tried separating things into batches. Meaning if i start this many plates, in this number of days they go to grandmaster jars, then mutiplied into masters, again into slaves, and by the time they ready to spawn i wud need to have already put new transfers on new plates for next "batch". Then i realized, why the hell am i doing this like the main goal is production? I have no need to, that is in no manner my "goal". In fact i thnk my goal is to learn and have fun doing it...and them BAM! Life got exponentially better.

It seems like life in our society had over time trained me to be productive, and that the highest level of production possible is success. That shit was just stuck in there. What a racket.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #26195878 - 09/18/19 02:12 PM (4 years, 4 months ago)

Quote:

GypsyCurse said:
I tried separating things into batches. Meaning if i start this many plates, in this number of days they go to grandmaster jars, then mutiplied into masters, again into slaves, and by the time they ready to spawn i wud need to have already put new transfers on new plates for next "batch". Then i realized, why the hell am i doing this like the main goal is production? I have no need to, that is in no manner my "goal". In fact i thnk my goal is to learn and have fun doing it...and them BAM! Life got exponentially better.

It seems like life in our society had over time trained me to be productive, and that the highest level of production possible is success. That shit was just stuck in there. What a racket.




I mean yeah, its all about having clean master jars really.  However if you dont back your shit up you can be easily out of action for  a month.  Im working too many mushrooms atm and need to cut back.  Id love it if agar was like a 5 day thing then u could be back in 2 weeks.


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: Edmunter]
    #26195914 - 09/18/19 02:39 PM (4 years, 4 months ago)

Quote:

Edmunter said:
Im working too many mushrooms atm and need to cut back.




I've been trying to work 5 strains at once and it's killing me.  But I can't cut back yet bc I only have prints for 2 of them.  I need to finish grows of the 3 other strains, get prints, then cut back to only working maybe 2 at a time.


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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: badsponge]
    #26195917 - 09/18/19 02:42 PM (4 years, 4 months ago)

Yeah, ya'll this multi-variety life is killing me. I feel you on this driving me nuts. Here's what I'm tracking right now (and this is just as a hobby, I have a day job)

On agar: 14 varieties across 3 species
On grains: 6 varieties across 2 species
In bins: 3 varieties across 2 species

:blowmybrainsout:


Edited by ShaperDreaming (09/18/19 02:43 PM)


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Offlinebadsponge
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26195922 - 09/18/19 02:45 PM (4 years, 4 months ago)

Holy shit, Shaper wins! :crazy2:


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: badsponge]
    #26195926 - 09/18/19 02:49 PM (4 years, 4 months ago)

No shit, makes me feel like I ain't doing nothing.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: ShaperDreaming]
    #26195927 - 09/18/19 02:49 PM (4 years, 4 months ago)

:carlinorgasm:  :brainscream:

Good to know im not alone!!!!

8 varieties - 4 species
4 varieties on grains - 2 species
2 varieties on dowels - 2 species
2 grows on chips - 2 varies - 2 species
1 variety on straw - 1 species



Edited by Edmunter (09/18/19 02:56 PM)


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Edmunter]
    #26195993 - 09/18/19 03:34 PM (4 years, 4 months ago)

I have.... quite a few projects going too


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: verum subsequentis]
    #26196048 - 09/18/19 04:10 PM (4 years, 4 months ago)

:tantrika:
God damn Sharper...
You dicked with black kow at all? I mixed a 5lb bag in after cooking the coir and am jst now seeing results from those shoeboxes. Holy kow! There is indeed a difference. RW & LGT with bK spawned later is pinning earlier. Growth coverage on surface signifigantly increased. Almost tossed a box cuz i thought there were patches of trich popping up, turned out to be pins.


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