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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Is it possible to breed genetics? [Re: tryptkaloids]
#26194665 - 09/17/19 08:11 PM (4 years, 4 months ago) |
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Hehe god damnit. For some reason my mind intermittently clears and im not a total dumb dumb for a short period. That was the best i cud do on such short intellectual notice. And there is only one question written there.
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SunnyDayze
Finder of shrubberies



Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
#26194681 - 09/17/19 08:22 PM (4 years, 4 months ago) |
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kirkland mixed nuts plastic jar. haven't had it cake on me yet and probably had it a year (just started using LME mainly this summer, preferred PDA)
Bonus, kirkland brand mixed nuts are awesome!
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Is it possible to breed genetics? [Re: SunnyDayze]
#26194701 - 09/17/19 08:41 PM (4 years, 4 months ago) |
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i'm not convinced nutrient specificity training is a thing. the assertion being that putting rinse/soak/cook water from the grain you intend to use into the agar media on which you're developing your cultures will somehow force the fungi to take to that grain more readily seems pretty thin. does the organism have to adapt to different grains by producing different enzymes or something? if it was switching between grass and wood, i'd believe it. between oats and rye, i'm doubting it.
however, i am not a smart mofo. i'm just a guy in a suit.
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the j stands for jesus.2020 new years grow along
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Is it possible to breed genetics? [Re: J. Jack Flash]
#26194719 - 09/17/19 08:57 PM (4 years, 4 months ago) |
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Right. You can temporarily change some growth characteristics of plated myc, and you can select a group of strains that on their own do well on a specific nutrient source, but training the myc to a particular nutrient source is futile.
Actually explains a lot on stuff happening with my cultures atm.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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ShaperDreaming said:
Caps: You got pics? I have this Golden Halo culture that has weird sectoring every time no matter what I do, or how small I transfer. It kinda drives me nuts.
I've really stopped taking pix... but I'll get some comparison pix when I do checks again: next couple days
One question with absolutely NO punctuation technically... outside of the needed "?"
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Is it possible to breed genetics? [Re: Caps McGee]
#26195009 - 09/18/19 04:54 AM (4 years, 4 months ago) |
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I think of it more like one source of nutes for too long causes an issue. Like when i eat mushrooms for a week and my stomach starts to produce a bunch of acid to break it down causing indigestion
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Is it possible to breed genetics? [Re: tryptkaloids]
#26195024 - 09/18/19 05:09 AM (4 years, 4 months ago) |
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Like it's good to swap it up. I wonder if it is indeed beneficial. In what way, and to what end.
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Yesum
Furry as Fuc



Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
#26195087 - 09/18/19 06:20 AM (4 years, 4 months ago) |
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Mines in a plastic Folgers coffee can. 
Still no chunking
Agar I keep more properly. More air tight
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Gen Disc [Re: Yesum]
#26195543 - 09/18/19 10:58 AM (4 years, 4 months ago) |
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Would slightly different malt extracts be enough to "change the nutes" for agar? Like a dark beer ME versus a light beer ME?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Bod told me once you can get the same results by changing nute %... He didnt seem bery sure of himself tho
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
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Re: Is it possible to breed genetics? [Re: tryptkaloids]
#26195575 - 09/18/19 11:18 AM (4 years, 4 months ago) |
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Yeah, I do that regularly, but I'm more thinking about this:
Quote:
tryptkaloids said: I think of it more like one source of nutes for too long causes an issue.
It's still the same nutes if you change the percentage, right?
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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I run MEA at 4g agar, 6g LME... don't change much, but I do a LOT of agar... I couldn't tell you if it's any faster or slower...
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Yes it is, but sometimes your culture just needs less nutes. That being said all any of us have is a guess. Ove read some people refer to their cultures getting lazy... That's likely not what's happening but people look for the best explaination
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Is it possible to breed genetics? [Re: tryptkaloids]
#26195591 - 09/18/19 11:24 AM (4 years, 4 months ago) |
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Seems like too much or too little either way will cause them to slow... but therein lies the operative: "seems like"
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tryptkaloids
Learner



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Re: Is it possible to breed genetics? [Re: Caps McGee]
#26195617 - 09/18/19 11:41 AM (4 years, 4 months ago) |
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Exactly, none of us know because each culture is different and diverse. Maybe verum, master of the isolates can lend a thought
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
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Re: Is it possible to breed genetics? [Re: Caps McGee]
#26195681 - 09/18/19 12:23 PM (4 years, 4 months ago) |
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Quote:
Caps McGee said: I run MEA at 4g agar, 6g LME
Per 1L or 500ml?
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jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
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Quote:
ShaperDreaming said:
Quote:
natedawgnow said: once a year or so I'll make a batch of PDA 
The first ever agar I made I completely winged it. I bought agar then looked up a recipe and was like "I don't have any of that shit..." so I boiled some potatoes for a half hour and used that water + molasses + agar
It totally worked too, but was hideous and the myc wasn't a huge fan, but it worked.
Speaking of LME: What the hell do you all store yours in? Mine keeps turning into a taffy-like substance before I even finish a bag of the shit. I keep it stored in a baggie, but I have that baggie in 3 other baggies at this point because the outside keeps getting coated in taffy-lme.
I want to put it into a container but not sure what would be best. I'm worried that anything with a seal to it might get some lme inbetween the gasket or something and it'll never open again.
Caps: You got pics? I have this Golden Halo culture that has weird sectoring every time no matter what I do, or how small I transfer. It kinda drives me nuts.
I know you have answers for the storing malt extract thing, but i have a really good one
Literally just vaccuum seal it. I put the whole pound from a fresh bag and put it on the vacuum sealer and its fucking perfect. When i need to use it, just cut the seal, weigh the amount, and reseal. Dummy simple. i store my agar in vacuum sealed bags too. Not that the vacuum part matters really, but the sealing is nice as hell
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 14 hours
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DO NOT i repeat DO NOT use dark malt extract. Its the worst shit in the world. I used to make agar form it and it only grew very weak transparent like mycelium.
I havent had a succesfull grow in my whole life (waiting some fruits within next week though) but from what i read on your agar recipe i believe you are using too much nutrients.
There are a a large amount of old threads of bodhisatta having arguments with another user Violet, about the amount of nutrients used in agar and how too much nutrition would appear to lead to tometose growth. That is why in the cases that people want to isolate mycelium from contamination they use low nutrient agar. Because it leads to a more rhizo growth. Your agar recipe has 4% nutrients, and almost every Trusted cultivator appear to recomend 2% from what i read. Thats my inexperienced advice based on everything i learned around here.
-------------------- How would you rate the quality of my answer?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
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Re: Is it possible to breed genetics? [Re: Sankhara]
#26195732 - 09/18/19 12:49 PM (4 years, 4 months ago) |
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i'm not the master of isolates, but The ultimate and final truth is, wait, what are we talking about. And who is changing the fucking thread titles? It's bothersome.
oh yeah, changing nutes and nute ratios. I've fooled around with it enough to have decided it's not worth fooling around with for cube cultivation. That being said, i still don't understand it. I just use 2% malt in all my agar nowadays though.
It's always the same with mycology on the hobby level. Peop
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Quote:
jbgtaa said:
Quote:
ShaperDreaming said:
Quote:
natedawgnow said: once a year or so I'll make a batch of PDA 
The first ever agar I made I completely winged it. I bought agar then looked up a recipe and was like "I don't have any of that shit..." so I boiled some potatoes for a half hour and used that water molasses agar
It totally worked too, but was hideous and the myc wasn't a huge fan, but it worked.
Speaking of LME: What the hell do you all store yours in? Mine keeps turning into a taffy-like substance before I even finish a bag of the shit. I keep it stored in a baggie, but I have that baggie in 3 other baggies at this point because the outside keeps getting coated in taffy-lme.
I want to put it into a container but not sure what would be best. I'm worried that anything with a seal to it might get some lme inbetween the gasket or something and it'll never open again.
Caps: You got pics? I have this Golden Halo culture that has weird sectoring every time no matter what I do, or how small I transfer. It kinda drives me nuts.
I know you have answers for the storing malt extract thing, but i have a really good one
Literally just vaccuum seal it. I put the whole pound from a fresh bag and put it on the vacuum sealer and its fucking perfect. When i need to use it, just cut the seal, weigh the amount, and reseal. Dummy simple. i store my agar in vacuum sealed bags too. Not that the vacuum part matters really, but the sealing is nice as hell
i bet you could weigh your powder out and seal the bag in sections so you can just cut a pouch out of the bag and avoid revealing it
Also, good catch on the % sankhara. 2% is even a little high for my taste. I didn't even think about looking at her recipe
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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