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InvisibleYesum
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Re: Is it possible to breed genetics? [Re: Feasoghorm] * 1
    #26193137 - 09/17/19 02:46 AM (4 years, 4 months ago)

No you shouldn't. I suppose you could.  But I'd say this is going above and beyond.

Keep it simple. Simple is good :super:


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InvisibleFeasoghorm

Registered: 10/24/18
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Re: Is it possible to breed genetics? [Re: Yesum]
    #26193146 - 09/17/19 03:03 AM (4 years, 4 months ago)

I thought i had jst seen myc that is not responding well at all to a change in food, which leads me to believe that consistancy in nutrient type wud be more favorable.

Are you simply meaning to express concern for over complication?


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InvisibleYesum
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
    #26193148 - 09/17/19 03:05 AM (4 years, 4 months ago)

Yes

I'm sure what you guys are referring to is true. What I'm saying is the difference its gonna make if any is negligible

And the mess and time your gonna spend adding the step of soaking/wringing out coir could be spent doing something fun or whatever

If you can implement into your schedule somehow so that it's not creating more work then I say go for it


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Edited by Yesum (09/17/19 03:14 AM)


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InvisibleFeasoghorm

Registered: 10/24/18
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Re: Is it possible to breed genetics? [Re: Yesum]
    #26193179 - 09/17/19 04:10 AM (4 years, 4 months ago)

Very proper of you bud. Thank you for clarifying.


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InvisibleYesum
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
    #26193182 - 09/17/19 04:16 AM (4 years, 4 months ago)

I aim to please

:puddingpop:


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OfflineFailboat
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Re: Is it possible to breed genetics? [Re: Yesum]
    #26193276 - 09/17/19 06:38 AM (4 years, 4 months ago)

I wouldn't worry about finding genetics that like to grow on coir, but selecting a culture that grows vigorously on your grain media would be ideal. Use grain water in your agar and you will be able to separate strains that will grow best on your grain choice as opposed to strains that just grow well on sugar.


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InvisiblebodhisattaMDiscordReddit
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
    #26193324 - 09/17/19 07:39 AM (4 years, 4 months ago)

Quote:

GypsyCurse said:
Rad dude, thnx for enligjtening me.

I suppose while we're at it... I was "speaking" with Ferather and he mentioned in terms that escape me atm, that it's more than reasonable to assume that myc propogated on some amount of whatever the myc wud be fruiting on wud greatly assist in the overall health of the grow, and/or wud to some degree produce a more favorable outcome. This true? Shud i be making MEA with coir tea?



Its a theory based in a little bit of facts. All biological systems regulate themselves based on their environment. You drink a lot you'll have more alcohol dehydrogenase. Mycelium doesn't make a bunch of enzymes if the substrate doesn't require them. But the idea that you make mycelium used to food to make it faster is bunk. The upregulation happens in minutes as a response to new foods/substrates or even stressors.


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Offlineverum subsequentis
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Re: Is it possible to breed genetics? [Re: bodhisatta]
    #26193866 - 09/17/19 12:45 PM (4 years, 4 months ago)

And, even if that were not true, you wouldn't make "coir tea". You'd want to make the tea from whatever the spawn was going to be. Remember the purpose of coir is to hold and supply water. The spawn has the nutes.

That being said, It's and exercise in futility, in my opinion


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OfflineShaperDreaming
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Re: Is it possible to breed genetics? [Re: verum subsequentis]
    #26193884 - 09/17/19 01:02 PM (4 years, 4 months ago)

Hey there ya'll. All this talk about agar has me thinking:

I haven't seen ropey myc growth on my plates in forever. I adjust the nutes and agar rates pretty regularly, everything from 5g agar + 5g LME + 500ml water all the way to 10g agar + 10g LME + 500ml water.

My current recipe is 7g agar + 8g LME + 500ml water.

I get great growth  of my myc that is usually 98%+ tomentose growth, occasionally I'll see ropey tips on my tomentose on the leading edge, but that's about it. The myc almost always goes rhyzo on grains and gets ropey then, but not on agar.

So I basically stopped giving a fuck about what type of myc I'm getting on agar about 6+ months ago... but I was thinking recently that it's a bit weird that I almost never see it any more. I still don't care mostly, but thought I'd get other people's input.


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Invisiblenatedawgnow
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Re: Is it possible to breed genetics? [Re: ShaperDreaming]
    #26193892 - 09/17/19 01:10 PM (4 years, 4 months ago)

Add something different to your agar.

I like to use lme and black strap molasses. 5-6g LME to 2-3g BSM is what I usually
use. Sometimes I use just malt, and once a year or so I'll make a batch of PDA :lol:

Changing up the nutes can help but ya super ropey myc doesn't really matter


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InvisibleCaps McGee
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Cultivation General Discussion [Re: Inocuole]
    #26193903 - 09/17/19 01:17 PM (4 years, 4 months ago)

Wish yall quit changing titles of shit... Post the comment, aint nobody reading the headers but me lol... Fucks with me; clickon one thing, read the title of another, go back bc its the wrong thread... Except naw, someone just changed the title again LMMFAO

Anyways... Question for yuns... I've been working with the peu white fairly extensively, and continue to get sectoring, despite some of them being clones of clones of cloned clones already... Are we certain a single strain is a thing? Am I seeing mutation occur after transfer? Some of them will lok mono acros 2-3 plates, and then branch again... Very peculiar; almost as if to unpack genetic code

I hardly ever see rhizo anymore... I speculate its bc the majority of the cubes I run are luecistic/albino varieties... Which outside of one APE culture I play with, are typically tomentose IME


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InvisibleStygianKnight
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26193927 - 09/17/19 01:31 PM (4 years, 4 months ago)

I don’t think anyone knows yet how exactly they determine which hyphae should be fussed with and who should be helped but not joined, and so on.

It’s entirely possible you’re seeing a set of cultures that are so inbred or otherwise related that they have a hard time telling each other apart from one another and only occasionally do.  On the opposite side of things it’s also possible the signaling system that causes fusion is unstable and prone to mutations or breakages and you have a monoculture that occasionally thinks itself as a different entity.


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OfflineShaperDreaming
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Re: Is it possible to breed genetics? [Re: natedawgnow]
    #26193991 - 09/17/19 02:14 PM (4 years, 4 months ago)

Quote:

natedawgnow said:
once a year or so I'll make a batch of PDA :lol:




The first ever agar I made I completely winged it. I bought agar then looked up a recipe and was like "I don't have any of that shit..." so I boiled some potatoes for a half hour and used that water + molasses + agar :rofl:

It totally worked too, but was hideous and the myc wasn't a huge fan, but it worked.

Speaking of LME: What the hell do you all store yours in? Mine keeps turning into a taffy-like substance before I even finish a bag of the shit. I keep it stored in a baggie, but I have that baggie in 3 other baggies at this point because the outside keeps getting coated in taffy-lme.

I want to put it into a container but not sure what would be best. I'm worried that anything with a seal to it might get some lme inbetween the gasket or something and it'll never open again.

Caps: You got pics? I have this Golden Halo culture that has weird sectoring every time no matter what I do, or how small I transfer. It kinda drives me nuts.


Edited by ShaperDreaming (09/17/19 02:17 PM)


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Offlinebadsponge
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Re: Is it possible to breed genetics? [Re: ShaperDreaming]
    #26193999 - 09/17/19 02:20 PM (4 years, 4 months ago)

Quote:

ShaperDreaming said:
Speaking of LME: What the hell do you all store yours in?




A plastic Gladware container. Mine develops kind of a hard surface that I just break up with a spoon, but doesn't get like taffy.


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Invisiblenatedawgnow
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Re: Is it possible to breed genetics? [Re: badsponge]
    #26194015 - 09/17/19 02:27 PM (4 years, 4 months ago)

Mason jar with a seal. I have some that I've had for a year in a jar and it's still powder.

If it gets gunked up pry it open with a butter knife or needle nose and put a
new lid on it. Never had it gunk up so bad that I couldn't open it though


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OfflineShaperDreaming
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Re: Is it possible to breed genetics? [Re: natedawgnow]
    #26194055 - 09/17/19 02:55 PM (4 years, 4 months ago)

So ... what I'm seeing points towards ... there's nothing that going to stop this monster material from becoming not-powder at least a little :lol: ok, got it. Sometimes I can't even get a spoon into the bag without it getting stuck on something going in and collecting a ton of new powder on the harder solid parts


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Offlineverum subsequentis
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Re: Is it possible to breed genetics? [Re: natedawgnow]
    #26194076 - 09/17/19 03:07 PM (4 years, 4 months ago)

Quote:

natedawgnow said:
Mason jar with a seal. I have some that I've had for a year in a jar and it's still powder.

If it gets gunked up pry it open with a butter knife or needle nose and put a
new lid on it. Never had it gunk up so bad that I couldn't open it though



:whathesaid:

i keep mine in a mason jar with two piece lid. When i open it to use it i use it quickly and close it back up. Also, never dip a wet spoon in it. Also, use a spoon and don't pour out of the jar. Following these basic principles, my malt lasts forever without issue.


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Offlinetryptkaloids
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Re: Is it possible to breed genetics? [Re: verum subsequentis]
    #26194108 - 09/17/19 03:29 PM (4 years, 4 months ago)

I don't think using a spoon matters as long as you wipe the rim off after. Ime anyway.

@caps. Im sure a single strain exists, i just think the only worthwhile way to go about it is with 2 spores


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleFeasoghorm

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Re: Is it possible to breed genetics? [Re: natedawgnow]
    #26194182 - 09/17/19 04:27 PM (4 years, 4 months ago)

Quote:

Quirkmeister92 said:
...selecting a culture that grows vigorously on your grain media would be ideal. Use grain water in your agar and you will be able to separate strains that will grow best on your grain choice...



Quote:

bodhisatta said:
The upregulation happens in minutes as a response to new foods/substrates or even stressors.



Quote:

ShaperDreaming said:
I haven't seen ropey myc growth on my plates in forever. I adjust the nutes and agar rates pretty regularly...



Quote:

natedawgnow said:
Add something different to your agar.





I really enjoy this thread. Thers some smart mofos in here.

I feel like i can reasonable deduce that while a change in nutrient type and/or strength may cause a temporary change in growth characteristics, it doesnt "train" the myc.
However there is such a thing as happening upon a set of genetics that do exceptionally well under specific nutrient conditions, to which there may be some benefit.

So "in real life" if a plated culture ends up being selected through several transfers for specific plate growth characteristics on a consistent nutrient type, changing the nutrient type may disrupt those characteristics but unless the plated media contained nutrients from the grain that will ultimately be the cultures main nutrient source, the futility arises when said culture is put to grain. Do i have that correct?


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Offlinetryptkaloids
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Re: Is it possible to breed genetics? [Re: Feasoghorm]
    #26194627 - 09/17/19 07:43 PM (4 years, 4 months ago)

Can you rewrite that last question? I need more commas and periods to make sense of that.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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