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InvisibleYesum
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #26173303 - 09/06/19 09:15 PM (4 years, 4 months ago)

:lol:

I can usually get a pretty nice looking t.1 plate from a clone like that.


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InvisibleFeasoghorm

Registered: 10/24/18
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Re: Cultivation General Discussion [Re: Yesum]
    #26173362 - 09/06/19 09:59 PM (4 years, 4 months ago)

Yesum you furry bastard, it's timbo, pm me. We have jerky to discuss.


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: Yesum]
    #26173371 - 09/06/19 10:10 PM (4 years, 4 months ago)

Quote:

Yesum said:
There just not fruits taken from a tub but rather a fruiting petri




Same and this is what I had in mind when I was asking the question. It was more of a hypothetical nature, it sort of just occured to me that my clone cultures were actually all different, since I'm not working with an isolate, and I was wondering if there was a way to to transfer a clone culture so that the resultant plate would contain an identical set of genetics.. Not that I would even care to do that, i was just blabbing.


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InvisibleYesum
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Re: Cultivation General Discussion [Re: junk_f00d]
    #26173591 - 09/07/19 04:33 AM (4 years, 4 months ago)

Mafukin gangster ๐Ÿ‘‡๐Ÿ‘‡๐Ÿ‘‡


This is an old pic but what I've been thinking about is when a culture looks like this yoda plate what to do.

Been wondering if I should also take a wedge from the section thats not leading incase characteristics  change for the worse.

I'm not even sure if that makes sense but that's what's been kicking around up there.


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Edited by Yesum (09/07/19 04:40 AM)


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: Yesum]
    #26173795 - 09/07/19 07:58 AM (4 years, 4 months ago)

Hypptheticals? You got time for demz?
:hyperlol:
I mean... an agar pin clone is one thing, and not exactly what I'd consider a "tissue sample"... idk, but I look at APC's as confirmation of at least a MOSTLY clean culture already... and typically, APC plates are clean... I still ALWAYS transfer out: even if I use the sample plate ... Whatever works for you


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26173800 - 09/07/19 08:05 AM (4 years, 4 months ago)

Are understanding what I'm asking? What's APC?


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OfflineLeftfield420
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Re: Cultivation General Discussion [Re: Yesum]
    #26173808 - 09/07/19 08:08 AM (4 years, 4 months ago)

Agar Pin Clone?


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: Yesum]
    #26173819 - 09/07/19 08:13 AM (4 years, 4 months ago)

Agar pin clone, yes... I  think like you said, taking the trunk of the split likely carries with it ALL genetics expressed further from that point, and that transfering from there rather than the leading edge would result in a wider array of genetics than said leading edge transfer: certainly... I'm just trying to figure out the utility of preserving less than ideal genetics, when one of the major purposes of transfering  (outside of cleaning the culture)is the isolation  of better performing genetics? I'm having a problem understanding what the purpose of trying to keep a clone as genetically variant as possible would be: just continue to run MS  :shrug:

An isolate and a clone are nowhere near the same thing guys... a clone from an MS fruit will still have likely hundreds, potentially even THOUSANDS of individual strain genetics... I understand the nature of the exercise, not it's utility


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Leftfield420]
    #26173822 - 09/07/19 08:14 AM (4 years, 4 months ago)

No. See how that yoda plate is showing 2 sectors. The majority of it, then that sector that's faster then the rest.
My question is should I take wedges from both areas, the leading edge and also from the edge that's not running fast.
Know what I mean? Or is this redundant

That's the thing though. Just because you take from the leading edge doesn't necessarily mean your getting better genetics. Fewer yes but not necessarily better.

We already know clones and isolates are different. I'm not sure where that came from.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: Yesum]
    #26173833 - 09/07/19 08:23 AM (4 years, 4 months ago)

I'd take both for observation personally... redundancy aside... just for science and my own knowledge... I like being thrown: discovering something to be completely counter to my initial belief... may be the case that the slower sector on agar is a beast on grain, or takes longer bc it establishes stronger, resulting in better yields... anything lol...  almost feel like I've ended up with something hidden when they do that... I always take them just in case, but haven't personally noticed discernable difference between performances of sectors like that vs the majority/remainder of the plate... :shrug:
Sure is fun though, and you NEVER know what you may run into!


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26173840 - 09/07/19 08:25 AM (4 years, 4 months ago)

Dude that was a lot of work just to find out if it makes sense to take wedges from both sectors

I love ya. But a lot of work. :lol:


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Invisiblerickyswamps
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Re: Cultivation General Discussion [Re: Yesum] * 1
    #26173847 - 09/07/19 08:33 AM (4 years, 4 months ago)

You hurt my feelings caps.  Gonna need some apology spores :cool:

Oh, and please donโ€™t worry about my panties.


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26173854 - 09/07/19 08:37 AM (4 years, 4 months ago)

Quote:

Caps McGee said:
An isolate and a clone are nowhere near the same thing guys... a clone from an MS fruit will still have likely hundreds, potentially even THOUSANDS of individual strain genetics...




Just learned something!  Thanks, Caps.  I had figured a clone was a single genetic isolate! 


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: Cthulhu23]
    #26173885 - 09/07/19 08:57 AM (4 years, 4 months ago)

Could I get your guys' opinion on this plate?



I'm planning on taking a transfer (after it grows out a teeny bit more) from the 3:00-9:00 areas, cuz that looks most like what I'm used to with myc.  I'm curious as to what you all think the big blobby white area is, as I have that on a number of the other transfer plates I have.  I've never grown PESA before, and the blobby thick area is how it looked on my first streak plate.  These are all GWA plates, for the record.

Also, do any of you have issues with major condensation on plates?  I have been running into this ever since I transferred my grow op from a walk-in closet to my basement.  I suspect it's due to the temperature differential (cool in the basement, warmer in the plate), and the fact I'm using pyrex plates?


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Cthulhu23]
    #26173896 - 09/07/19 09:04 AM (4 years, 4 months ago)

Condensation can be a problem. Loose water rolling over your plate will carry and contams present with it.

When i see a plate like that, i can never decide if that white spot is contamed, or just stronger. I would let it go another 2 days or so and see what your leading edges develop into to find a strong healthier section. Right now everything just looks weak and trying to get going to me.


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: LtLurker]
    #26173903 - 09/07/19 09:11 AM (4 years, 4 months ago)

Any ideas on how to mitigate the condensation?  Maybe just have my plates in the closet vs the basement?


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InvisibleMycolorado
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Re: Cultivation General Discussion [Re: Cthulhu23]
    #26173906 - 09/07/19 09:15 AM (4 years, 4 months ago)

Stacking helps.


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Invisiblerickyswamps
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Re: Cultivation General Discussion [Re: Cthulhu23]
    #26173914 - 09/07/19 09:22 AM (4 years, 4 months ago)

Quote:

Cthulhu23 said:
Could I get your guys' opinion on this plate?



I'm planning on taking a transfer (after it grows out a teeny bit more) from the 3:00-9:00 areas, cuz that looks most like what I'm used to with myc.  I'm curious as to what you all think the big blobby white area is, as I have that on a number of the other transfer plates I have.  I've never grown PESA before, and the blobby thick area is how it looked on my first streak plate.  These are all GWA plates, for the record.

Also, do any of you have issues with major condensation on plates?  I have been running into this ever since I transferred my grow op from a walk-in closet to my basement.  I suspect it's due to the temperature differential (cool in the basement, warmer in the plate), and the fact I'm using pyrex plates?




If the condensation is from when you poured them, then the agar was too hot.  Could be what your saying about temp diff, but takes quite a swing.

Iโ€™ve noticed if I transfer from a plate like that , itโ€™ll sort itself out and look a lot better.  Guess you could take 2 transfers from those different looks.


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: rickyswamps]
    #26173966 - 09/07/19 10:04 AM (4 years, 4 months ago)

They were super clear after pouring; it was after the transfers and moving them to the basement when the condensation really occurred.  Itโ€™s probably 5-10 degrees F cooler in the basement than in the closet.  Plus theyโ€™re Pyrex dishes which could be part of the problem (glass seems to condense more than plastic IME - more heat transfer I would assume).

Iโ€™ll try storing them in the closet and see if that makes a difference.

Thanks for the advice , guys!


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: Cthulhu23]
    #26174032 - 09/07/19 10:43 AM (4 years, 4 months ago)

temp swings like that will absolutely cause condensation. I'm not sure about glass doing it more than plastic though.

Yesum, I don't think any living soul knows the actual truth about some aspects of this shit. Too many variables and too few documented tests. I've often wondered about this and have often taken from as many parts of the culture as look appealing to me. Of course, this is how you end up with an ungodly wall of petris. I've never found the truth though.

Best and simplest solution, take from as many places as you want and slam them into the same plate. I've done that a time or ten.

I've run the tests on each sector also a time or ten but have nothing to report. Too many variables. Not enough attention payed to the details.


Edited by verum subsequentis (09/07/19 10:45 AM)


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