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InvisibleR.I.P.Zappa
Myco Melyco


Registered: 03/30/18
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Re: Cultivation General Discussion [Re: StygianKnight]
    #26164276 - 09/01/19 06:47 PM (4 years, 4 months ago)

Been hypothesizing that if the more blue they stain when cutting,they have more Psilocin present that gets lost in the drying process, as to were the ones that barely blue have more of the stable psilocybin and retain there original potency in the end.

Meaning the ones that blue more easy/quickly may be good fresh eating candidates and ones that blue less hold there original content after the drying process.

I don't see blueing as a indicator of how potent they will be after drying but more so how potent they may be fresh. It's nothing I rack my brain over or get concerned about but just one of those passing thoughts.  :whistling:


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

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OfflineLtLurker
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Re: Cultivation General Discussion [Re: R.I.P.Zappa]
    #26164294 - 09/01/19 07:00 PM (4 years, 4 months ago)

I can't wrap my head around how that'd be related at all. Blueing is some kind unrelated compound found in non-psychs as well. How would it indicate something different about the psilocybin inside? What could be different that removal of water removes additional psilocybin along with it?

More at the root, are you just high homey?


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Offlinetryptkaloids
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I'm a teapot


Registered: 02/08/15
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Re: Cultivation General Discussion [Re: StygianKnight]
    #26164307 - 09/01/19 07:05 PM (4 years, 4 months ago)

Quote:

StygianKnight said:
There have only been a few studies of potency degradation and none have been followed up last I heard.

There are a few things we can assume could be an issue though.  Psilocin, the dephosphorylated version of psilocybin is pretty unstable in comparison and in ambient or above temperature atmosphere will degrade over time.

Dephosphorelating enzymes exist in mushroom cells for a variety of reasons including converting ATP into ADP to do work similar to what happens in your own cells, it’s also only active in water.  So for unknown reasons, maybe cell rupture, these enzymes chill in a wet bath with your psilocybin, they will eventually eat some, convert it to psilocin and that will then further degrade when dried, losing you potency.  Though this is all a hypothetical as there’s no evidence this is what’s happening, just that this is a potential issue around left damp shrooms.

(While alive mushrooms have an enzyme that will spend ATP to phosphorylate tryptamines it finds, so any accidentally converted psilocin will get turned back into the more stable psilocybin.)



Mushrooms can stay alive for awhile after picked, does this mean degregation is negated.?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26164343 - 09/01/19 07:32 PM (4 years, 4 months ago)

In theory yes, for a time, refrigeration can also slow or stop a lot of enzymatic action, so there’s certainly a buffer window.

These enzymes not only don’t work when dry but may denature with warm heat.  Mushrooms that appear to rehydrate after being dried could be losing a large amount of potency by bursting out these still active enzymes and letting them slowly work through the mushy.  Making sure that last bit of water is gone before storage might be crucial to long lasting potency.


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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
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Re: Cultivation General Discussion [Re: StygianKnight]
    #26164353 - 09/01/19 07:38 PM (4 years, 4 months ago)

Oxidation requires oxygen, and could be minimized by placing them in a paper bag in the fridge... that said, fresh mush is 90% water, and water is 33% oxygen... not sure if it plays a role, or would even be accessible to lend to oxidation (not a chemist)... Heated dehydration is thought to destroy the enzymes in the mushrooms that breakdown psilocybin and psilocin over time... why I always dry at 165F for a full 24hrs... exposure is minimal, and covers all the bases for evem the largest of fruit...like quirk said,  generally anecdotal evidence at best

Properly store your cracker dry mushrooms in a vac seal bag, or unmodified canning jar


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26164360 - 09/01/19 07:46 PM (4 years, 4 months ago)

Quote:

Oxidation requires oxygen


Not necessarily. Oxidation is the loss of electrons and can be caused by lots of things, the hydrogen in the water is probably more harmful


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinePreparationH
apply daily

Registered: 03/28/05
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26164386 - 09/01/19 08:06 PM (4 years, 4 months ago)

OILRIG

Oxidation is Loss
Reduction is Gain

of electrons


I will never forget that shit REEEEEEEEEEEEEEE


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: PreparationH]
    #26164409 - 09/01/19 08:31 PM (4 years, 4 months ago)

Fluorine is a stronger oxidiser than oxygen


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Offlinevinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26164479 - 09/01/19 09:07 PM (4 years, 4 months ago)



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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: vinnie boombotz]
    #26164821 - 09/02/19 07:18 AM (4 years, 4 months ago)

the H2 concentration of fresh HRW was 1.0 mM in a hermetic canister. When the canister was opened, the H2 concentration fell to 0.8 mM in 30 min. The H2 concentration was maintained at 0.4 mM in 25 °C for at least 12 h.

So the HRW comes in at 1nm. Hydrogen is 2g/mole (H2)
So all calculated out that's 2ppm hydrogen
But it falls to 1.6ppm in 30m
And they're able to keep it at 0.4nm or 800ppB for the experiment
(Hydrogen is extremely poorly soluble in water)
The 25% HRW was considered to work well which would have had at most 200ppb hydrogen available but hydrogen being the smallest molecule possible effuses extremely readily (even right through plastic containers)

Then the sample size was too small to achieve results you could call significant.

So idk there is nothing compelling about that article to me until more people do it


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: bodhisatta]
    #26168631 - 09/04/19 11:27 AM (4 years, 4 months ago)

Does anyone use straight sterilized coir as a substrate?


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OfflineEl Chupacabra
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Registered: 01/07/19
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Re: Cultivation General Discussion [Re: Edmunter]
    #26168638 - 09/04/19 11:31 AM (4 years, 4 months ago)

Probably most people do. Maybe not necessarily sterilized tho.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: El Chupacabra]
    #26168666 - 09/04/19 11:44 AM (4 years, 4 months ago)

Quote:

El Chupacabra said:
Probably most people do. Maybe not necessarily sterilized tho.




I use pasteurized but sterilised would be allot easier.  Im so busy that even those few minutes checking the thermometer could be used elsewhere


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OfflineFailboat
Fuck Up

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Re: Cultivation General Discussion [Re: Edmunter]
    #26168670 - 09/04/19 11:46 AM (4 years, 4 months ago)

That's what bucket tek is for


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: Failboat]
    #26168709 - 09/04/19 12:11 PM (4 years, 4 months ago)

Sterilized is unnecessary.  And yes, many of us use straight coir that’s been pasteurized (I use bucket tek).


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Edmunter]
    #26168710 - 09/04/19 12:12 PM (4 years, 4 months ago)

Quote:

Edmunter said:
Does anyone use straight sterilized coir as a substrate?




i made a tek about doing that
:smuglook:


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Offlineverum subsequentis
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Re: Cultivation General Discussion [Re: mushboy]
    #26168724 - 09/04/19 12:24 PM (4 years, 4 months ago)

How in the balls could it be easier than dumping boiling water on coir? Thermometer checking is not needed. I personally like to do ten or so at a time in a big ass "tough tote". I've done the Thermo checking. The stays way above temp for way over an hour.

Dump, lid, and walk away. Pretty easy


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OfflineCthulhu23
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26168768 - 09/04/19 12:55 PM (4 years, 4 months ago)

Quote:

verum subsequentis said:
How in the balls could it be easier than dumping boiling water on coir? Thermometer checking is not needed. I personally like to do ten or so at a time in a big ass "tough tote". I've done the Thermo checking. The stays way above temp for way over an hour.

Dump, lid, and walk away. Pretty easy




:whathesaid:


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: verum subsequentis]
    #26168980 - 09/04/19 03:23 PM (4 years, 4 months ago)

Quote:

verum subsequentis said:
How in the balls could it be easier than dumping boiling water on coir? Thermometer checking is not needed. I personally like to do ten or so at a time in a big ass "tough tote". I've done the Thermo checking. The stays way above temp for way over an hour.

Dump, lid, and walk away. Pretty easy




But thats sterilising it to a certain extent or am I just stoned again?


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Edmunter]
    #26169001 - 09/04/19 03:34 PM (4 years, 4 months ago)

That's partial sterilization i think.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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