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Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ


Registered: 06/13/09
Posts: 4,825
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Re: Cultivation General Discussion [Re: vikingsc]
#26141976 - 08/18/19 07:14 PM (4 years, 5 months ago) |
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colonizing grain jars are sitting at 60f during the day, probably 55f during the night. These temps too low for proper colonization?
-------------------- ~ I used to get high on life, until I realized life was cut with morons ~ * You need 2 wake up and smell the music! * -We are all computer data in a materialistic world- |Sometimes you have to lose yourself, to find anything|
 
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verum subsequentis
seeker of truth



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Re: Cultivation General Discussion [Re: Blazer420]
#26141985 - 08/18/19 07:25 PM (4 years, 5 months ago) |
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not perfect but it'll work. just a little slower
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Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ


Registered: 06/13/09
Posts: 4,825
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Alright thanks. hot water cupboard is sitting @ 65f. but in darkness... I put 1 jar in there just to test the waters.
-------------------- ~ I used to get high on life, until I realized life was cut with morons ~ * You need 2 wake up and smell the music! * -We are all computer data in a materialistic world- |Sometimes you have to lose yourself, to find anything|
 
Edited by Blazer420 (08/18/19 08:49 PM)
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
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Loc: Equestria? Mordor? Wester...
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26142120 - 08/18/19 09:26 PM (4 years, 5 months ago) |
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Quote:
tryptkaloids said:
Quote:
mushboy said:
Quote:
MLPismyOPSEC said:

Imo agar is too soft
density of agar definately effects the availability of nutes. That's why i don't like premixed agar, that way i can tweak it to make it richer or softer batch by batch
Its not one size fits all, each set of genes has different "preferences"
I didn't get any thick, rhizo growth like PMyst-5-1-1 when i was using 10g agar/500mL, mostly had growth more like Arg-5-2. Switching to 7.5g/500mL (which all three in that pic are) seemed to help get thicker growth, and faster growth. That, along with the other comments about adaptation to MEA, i'm ready to switch over to PDA/PDY or some other recipe and see what happens.
Edited by MLPismyOPSEC (08/18/19 09:28 PM)
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Subfinder
Wildlife enthusiast


Registered: 07/01/18
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Re: Cultivation General Discussion [Re: vikingsc] 1
#26142372 - 08/19/19 05:18 AM (4 years, 5 months ago) |
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Quote:
vikingsc said: Okay, so I got a couple dozen prints in the mail last month. One of them the two letter acronym on it is 'BC'. I can't think of what strain that would stand for. Any idea?
Brazil Corumba
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: Subfinder]
#26142442 - 08/19/19 07:10 AM (4 years, 5 months ago) |
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Quote:
Quirkmeister92 said: Ok so what I said stands, right? You recommend agar containing starch and cellulose.
Yes, when it comes to multiple genetics (spores). Sugar is fine as long as it's being spent to decay more complex materials, such as cellulose. You want gene expression on a media (in this case agar), that contains nutrients from your end media (substrate).
This will allow you to visibly select strains that do well on your agar media (end substrate).
Quote:
Quirkmeister92 said: As for the coco coir you're saying it is more efficiently consumed by mycelium if you adjust the pH? The maximum yield page was kinda iffy.
Not quite what I was saying, coir is a composition of lignin, phenols and cellulose (carbon sources), it's basically wood.
Cubensis normally fruits from dung (manure), which is neutral to alkaline, not acidic (or mildly). Since phenols are acidic, adding calcium increases the pH (normally already done).
Ultimately pH effects enzyme stability, pH and can also separate strains.
http://docsdrive.com/images/ansinet/ja/2010/tab1-2k10-92-101.gif http://www.scielo.br/img/fbpe/bjm/v32n3/7732f1.gif
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Manure is up to 40% cellulose, most of the starch and sugar has gone (if ever present).
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@ MLPismyOPSEC
Good luck.
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Failboat
Fuck Up

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Re: Cultivation General Discussion [Re: Ferather]
#26142547 - 08/19/19 09:05 AM (4 years, 5 months ago) |
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Oh ok so a good coir has already been neutralized/alkalized as Max Yield explained. It mentioned that this increases tensile strength. Is that relevant for our use, or just for growing plants? You still reccomend increasing the pH regardless, or just in some coir?
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ShaperDreaming
Weirdo



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Re: Cultivation General Discussion [Re: natedawgnow]
#26142667 - 08/19/19 10:37 AM (4 years, 5 months ago) |
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Quote:
natedawgnow said:

Usually I like to do wbs to wheat bags but I like the way the mixed grains perform so I been doin it like that the past few runs
Hey, what grain prep techniques do you use? I just switched to millet and rye, and my first few attempts to do rye have had about <5% burst grains, but that still seems like too much. I tried the same technique twice, second time using lower temps and a slower boil, same results. I'm worried that my millet will do the same thing, and burst millet sounds like a fucking mess.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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@ Quirkmeister92
You should check the pH if you can, and make your own adjustments. The tensile strength will mostly be due to solid calcium particles interweaving with the coir fiber. The pH will depend on your strain, ideally you should know what pH is better suited to your strain (like my L.edodes examples).
If for example, best growth was observed at pH 7, then ideally the coir should also have a pH of 7.
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I've grown cubensis on 100% calcium carbonate rocks, which have a pH of 9.9. Manure is generally not acidic, so I'd say the range is pH 6-9.


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Extract from my journal:
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Enzymes (General):
Enzymes are a type of protein, they are composed of C-H-N-O-(Other), the composition of enzymes varies. Disclaimer: Optimal temperature and pH varies with the organism, and it's enzymes.
Cellulase > Cellulose: 4.5-8.5. Amylase > Starch: 3.0-7.0.
Protease > Protein: 2.5-11.
Laccase > Phenols: varies. Peroxidase > More here.
For cellulase there are four types:
Alkaline cellulase: 7.2-8.5. Neutral cellulase: 6.0-8.0. Hybrid cellulase: 4.5-7.0. Acid cellulase: 4.5-5.0.
For amylase there are three types:
α-Amylase: 6.7-7.0. < Any amount of glucose, from any location. β-Amylase: 4.0-5.0. < Cleaves two units at a time, location specific. γ-Amylase: 3.0. < Cleaves up to two units?, location specific.
Laccase remains stable at pH 5.0-10.0. < Has a high pH tolerance.
For protease there are three types:
Alkaline protease: 7.0-11.0. Neutral protease: 6.0-9.0. Acid protease: 2.5-4.0.
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Cultivation General Discussion [Re: Ferather] 1
#26142781 - 08/19/19 12:03 PM (4 years, 5 months ago) |
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Quote:
ShaperDreaming said:
Quote:
natedawgnow said:

Usually I like to do wbs to wheat bags but I like the way the mixed grains perform so I been doin it like that the past few runs
Hey, what grain prep techniques do you use? I just switched to millet and rye, and my first few attempts to do rye have had about <5% burst grains, but that still seems like too much. I tried the same technique twice, second time using lower temps and a slower boil, same results. I'm worried that my millet will do the same thing, and burst millet sounds like a fucking mess.
For millet (white) I soak it over night for 12-14 hours then load it wet. Got this from mushboys method. There is a simmer method that if I remember correctly hamloaf posted but I found the soak and wet load from mushboy to be the most consistent on results. Millet also expands around 75% of it's original size after the PC cycle so be mindfull on how much you load your jar with it.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Cultivation General Discussion [Re: R.I.P.Zappa] 1
#26142937 - 08/19/19 01:58 PM (4 years, 5 months ago) |
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 50lb Rye, 50lb Winter Wheat, 50lb White Millet, 38lb Milo, 1 bale of straw. Lucked out on the milo as the only full bag was apparently dumpster grade so she gave me the opened bag plus the straw.
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ShaperDreaming
Weirdo



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Re: Cultivation General Discussion [Re: R.I.P.Zappa]
#26143039 - 08/19/19 02:54 PM (4 years, 5 months ago) |
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Quote:
Quirkmeister92 said:
 50lb Rye, 50lb Winter Wheat, 50lb White Millet, 38lb Milo, 1 bale of straw. Lucked out on the milo as the only full bag was apparently dumpster grade so she gave me the opened bag plus the straw.
   
Kick ass. I can't wait to hear your reports. I've heard that Milo is a little slower to colonize. I have a 25# bag sitting around that I can't bring myself to use as I'm all about speed these days. I'm excited to see what you come up with.
Did you see how I break down my bags? Just an idea
Also Last time I bought a bale of straw it ended up going to seed on me before I could use/break it down Unusable to me at that point since it was totally nasty in the center of bale when I got to it.
There's a place here in the city that sells it in a huge contractor bag w/o any baling so it's easier to keep inside your house. I'm going to go there when it's closer to oyster season.
Quote:
R.I.P.Zappa said: Millet also expands around 75% of it's original size after the PC cycle so be mindfull on how much you load your jar with it. 
You mean 175%? Also is that 175% from when you take it out of the soak or total from dry? Thanks!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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That's a really nice mix of grain and seed. Good luck, I like small bird seed, but I'm all for DIY mixes.
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Crackatoa
Stranger in a strange land



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Re: Cultivation General Discussion [Re: Ferather]
#26143121 - 08/19/19 03:48 PM (4 years, 5 months ago) |
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My oats have been drying for 48 hours in my fridge. I hope they're good, PCing after work.
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Here is a visual and it expands a little over double in volume from the start. This is with white millet, never worked with just millet but have been told the prep is relatively the same between the two. Should have gotten a more level shot of the jar but I'm sure you'll get the relative idea. The black line is were it would be at dry. Red line after 12-14 hour soak. The blue line after pc run. (mabye just a tad under the blue line)
 
Here is the Tek from mushboy that I've stuck with. Mushboy's bird seed tek
This is what I do to stay consistent with measurement after trail and error. If I'm making pint masters I use a 1/2 pint jars and fill it a 1/2 inch below where the threads start. Quart masters: then I use a pint and fill that below the shoulder before it angles up to the threads. Than I throw it in a full pint/quart jar with water to soak, next day, empty the water and grain into a strainer then reload the pint/quart jars and pc. I use 2 pints for 7 oat jars and the next two millet masters. Also the reason for 2 pints instead of a quart is just incase I get a ride along mold or if I get bacteria, I still have the other to noc up the next two masters and a couple oat jars.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Cultivation General Discussion [Re: R.I.P.Zappa]
#26143508 - 08/19/19 08:08 PM (4 years, 5 months ago) |
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Just tried boiling milo and I think I'm gonna just throw it out so much exploded. This whole bag was not only dirty but had bugs. The triple rinse hamloaf recommends took out the bugs well enough, but unfortunately they left behind half eaten grain that will certainly starch the rest. As for the other 20+lb i am soaking it overnight so as to not fuck it up any worse. Shit's so cheap I'm not gonna sweat it. It was the very last of their supply so I'm not suprised by the infestation. Hate to think of how bad the bag she wouldn't sell me was lol I guess I'll be running bags tomorrow
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Quote:
ShaperDreaming said:
Quote:
natedawgnow said:

Usually I like to do wbs to wheat bags but I like the way the mixed grains perform so I been doin it like that the past few runs
Hey, what grain prep techniques do you use? I just switched to millet and rye, and my first few attempts to do rye have had about <5% burst grains, but that still seems like too much. I tried the same technique twice, second time using lower temps and a slower boil, same results. I'm worried that my millet will do the same thing, and burst millet sounds like a fucking mess.
Millet is probably the hardest grain to fully hydrate. Wheat and rye absorb water really easily, but I've had millet soak 12 hrs and still be hard.
I prep all my grains via foomans method. Basically just dump boiling water over my grains and let them soak for a few hours, sometimes overnight if using pure wbs. Before loading I dump out the water, and pour in some more boiling water, let soak for a literal minute, then dump out to steam dry.
If I'm doing bags I let it dry til dry on the outside and for jars I load with just a teeny bit of moisture.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: natedawgnow]
#26143841 - 08/20/19 02:12 AM (4 years, 5 months ago) |
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That's exactly how I do my grain as well, nate, but I have them in HD buckets inside mesh paint strainers. That way I can lift them out and set them into strainers without dumping out, spilling some, etc. It's much easier.
I have a bucket with tons of holes drilled in the bottom that I use to strain my WBS ahead of time, leaving behind the sunflower seeds and corn. Overall my oats see about an 82% increase in weight after fan drying and my WBS (millet, sorghum) is around 38%.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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----
Here is some info, seeds have a shell, unlike 'naked' grain. The composition of seed shells varies, however they contain:
Lignin (and other phenols), holocellulose, hemicellulose and cellulose (all carbon sources).
If we compare brown, and white rice (more here), brown does much better.
Note: Brown rice contains roughly the same amount of nitrogen as composed manure.
Edited by Ferather (08/20/19 05:58 AM)
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