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OfflineFerather
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Re: Cultivation General Discussion [Re: bodhisatta]
    #26136734 - 08/15/19 07:32 AM (4 years, 5 months ago)

"Contrary to "conventional" growth by cell division, hyphae grow nearly infinitely (similar to human nerve cells) by extension at their tips." -- Source
"The fruit-body is a specific fungal organ that differentiates in order to produce spores and further ensure spore dispersal ..." -- Source

Fruit tissue (especially caps) can also act like lungs, and perform other chemical reactions (example: light, uv).


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OfflineMike O.Rrhizal
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Re: Cultivation General Discussion [Re: bodhisatta]
    #26136738 - 08/15/19 07:38 AM (4 years, 5 months ago)

Quote:

bodhisatta said:
Quote:

Mike O.Rrhizal said:
As fa as ive understood mushrooms need so mush water to fruit because the mushroom itself because they grow through cell expansion not through cell division. So the mycelium
will form the pin will all the cells that the mushroom needs to mature then it will fill these cells with water to grow the mushroom. And the mycelium cannot store water so can only use the water directly available to it.



There's cell division in a growing mushroom




Hey bod. you're right mushrooms do grow with cell division.

Ive just gone and read the info got this from and realised I should be saying cell elongation not cell expansion.

I was just pointing out that the mycelium uses a lot of water when fruiting because some of the cells especially In the stipe use cell elongation to grow too. And they elongate by filling with water, making the fruit mostly water.

Heres someone explaining it better, I don't understand it 100% so am probably wrong somewhere.


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OfflineFerather
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Re: Cultivation General Discussion [Re: Mike O.Rrhizal]
    #26136747 - 08/15/19 07:44 AM (4 years, 5 months ago)

You would have been correct if the subject was mycelium, not fruit tissue.


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Ferather]
    #26136753 - 08/15/19 07:52 AM (4 years, 5 months ago)

Mycelium and fruits grow by elongation and division. Not mutually exclusive of each other


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OfflineFishLevelMidnight
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Re: Cultivation General Discussion [Re: bodhisatta]
    #26136812 - 08/15/19 08:37 AM (4 years, 5 months ago)

Think of it this way, you have to divide to produce all those cells that will then elongate after filling with water and by increasing cell size or whatever

Plants are like this too, that is why a small bud can become a leaf so quickly.

Or also kinda similar to how fly wings are produced (imaginal disks are formed in the larvae)


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OfflineMike O.Rrhizal
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Re: Cultivation General Discussion [Re: bodhisatta]
    #26136842 - 08/15/19 09:00 AM (4 years, 5 months ago)

I see. I had understood there was a difference.

Learning everyday  :drinkcoffee: :takingnotes:


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OfflineTight Lunchbox
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Re: Cultivation General Discussion [Re: Mike O.Rrhizal]
    #26137325 - 08/15/19 03:51 PM (4 years, 5 months ago)

Does anyone on here use the plastic lids from fungi perfecti?

Mine showed up recently and I checked to see if they would seal just out of curiosity. I tightened a lid on to a jar of water and it seems the lids seal perfectly, like I couldnt get a drop of water to leak out by shaking the fuck out of it or turning the jar upside down.

Im going to pc a jar of water this weekend  and see if it warps from the heat.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: Tight Lunchbox]
    #26137330 - 08/15/19 03:55 PM (4 years, 5 months ago)

They’re probably just marked up ball lids. Seems to be paullies style. Slap a FP sticker on it and add 25-50%


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OfflineTight Lunchbox
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Re: Cultivation General Discussion [Re: AyePlus]
    #26137355 - 08/15/19 04:13 PM (4 years, 5 months ago)

They're for sure not the same as ball lids but I know where you are coming from.


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: Ferather]
    #26137359 - 08/15/19 04:17 PM (4 years, 5 months ago)

Quote:

Ferather said:
It might be the light but the two T3's look more visible than the T2.




Right and that's why i was asking. Bottom right is the most visible and the most rhizo, with bottom left being equally visible but more tomentose, and then T2 being highly faint. With a light behind it, it looks rather rhizo, just quite transparent, hence the question. If i keep transferring the T2, it should start to get more visible, correct?


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OfflineFerather
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #26138042 - 08/16/19 06:31 AM (4 years, 5 months ago)

Yes that sounds like adaption. You would be better off transferring to grain water agar, ideally the grain you intend to use as spawn.
Any adaption that takes place can be isolated (if possible), either way it should do very well transferred to grain.

Malt extract is made by starch (grain) + enzymes, ideally the mycelium should do this it's self.

====

More info on mycelial cell expansion:

Mycelium (Note: Fungal Growth on Solid Substrates) **
Fungal cell wall chitinases and glucanases

** Cubensis can also synthesize polysaccharides.

====

Found some interesting data in relation to the calcium at the tip of mycelium, in some cases it's to do with fungal oxalates.
In other cases it might be to do with the formose reaction, which uses formaldehyde to produce simple sugars.

"Among the Basidiomycota, two strains produced oxalic acid and one strain produced gluconic and formic acid." -- source

Analysis of Formose Reaction Products by LC–UV (ultraviolet).

https://en.wikipedia.org/wiki/Chemosynthesis

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Cubensis burma, on T-Gel (tea phenols), no sugar, no starch, no cellulose (no glucose sources), see here.

           

It finalized oxidation, and absorbed-converted materials, into sugars-other.

====

http://docsdrive.com/images/ansinet/ja/2010/tab1-2k10-92-101.gif


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Edited by Ferather (08/16/19 09:05 AM)


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: Ferather]
    #26139113 - 08/16/19 08:49 PM (4 years, 5 months ago)

Quote:

Ferather said:
Yes that sounds like adaption. You would be better off transferring to grain water agar, ideally the grain you intend to use as spawn.
Any adaption that takes place can be isolated (if possible), either way it should do very well transferred to grain.




Really? Even after just one/two transfers from a spore swab? Seems nuts to me but i suppose the swab could have been from mushies grown on MEA in the past, and the mycelium "remembers" that (for lack of better words).


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #26139580 - 08/17/19 07:21 AM (4 years, 5 months ago)

Genetic memory lasts much longer than that.


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OfflineFerather
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26139642 - 08/17/19 08:24 AM (4 years, 5 months ago)

ELME - Extra light malt extract (still malt extract), same sugars so on, made from grain + enzymes.

https://www.homebrewersassociation.org/how-to-brew/how-malt-extract-is-made/


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #26139645 - 08/17/19 08:26 AM (4 years, 5 months ago)

I'll be damned. Guess i'll have to try another recipe! Shame i just prepped grain a week ago, didn't even consider switching from MEA yet since i'm still so early on in my myco career. I'll probably go with PDA, seems to be one of the easier switches.

This tells me it's not strictly media based, but i will try new media and see how it goes.


Edited by MLPismyOPSEC (08/17/19 08:27 AM)


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OfflineFerather
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #26139680 - 08/17/19 08:47 AM (4 years, 5 months ago)

How many transfers after the spore plate is the top 1, and is the top one also ELME?
Looks like multi-genetics, with adaption to media (better genetics).

Uniform genetics, should produce consistent growth.

----

The downside at the moment is the genetic adaption will be ELME based.


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Offlinedmppb
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Re: Cultivation General Discussion [Re: Ferather]
    #26139862 - 08/17/19 11:20 AM (4 years, 5 months ago)

i've been getting this bright orange mold contamination on my straw bags , only in the slit parts , wtf is this ?



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InvisibleMycolorado
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Re: Cultivation General Discussion [Re: dmppb]
    #26140067 - 08/17/19 02:21 PM (4 years, 5 months ago)

Ha, quit putting pinks in them bags!  :lol:


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: Ferather]
    #26140091 - 08/17/19 02:43 PM (4 years, 5 months ago)

Quote:

Ferather said:
How many transfers after the spore plate is the top 1, and is the top one also ELME?
Looks like multi-genetics, with adaption to media (better genetics).




Top one is first transfer, bottom two are transfered from the top plate. All are ELME, same recipe as in my last post.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #26140130 - 08/17/19 03:05 PM (4 years, 5 months ago)

Dropped a jar and tore the cloth fins on my HEPA, will this be a problem?




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