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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
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Re: Cultivation General Discussion [Re: BigSkyMind] 1
#26134204 - 08/13/19 05:19 PM (4 years, 5 months ago) |
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Every single page has a banner on top with the words "support our sponsors". could not be easier.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: BigSkyMind]
#26134213 - 08/13/19 05:25 PM (4 years, 5 months ago) |
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Quote:
BigSkyMind said: Can someone refer me to a source where I can buy spore syringes and/or spore prints? I have spent an inordinate time on this site without finding such. One page refers to the site's "vendors" and "sticky" threads, but I can find no vendors or any way to make spore syringes without already having mushrooms on hand. Thank you!
Come on now. The sticky post is the only post stuck to the top of the list of threads
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Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ


Registered: 06/13/09
Posts: 4,825
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Re: Cultivation General Discussion [Re: bodhisatta]
#26134543 - 08/13/19 09:27 PM (4 years, 5 months ago) |
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Anybody think that this is mycelium growing in this spore syringe?? Looks like a white fluffy cloud surrounding spores? I made these syringes over a month ago and I just noticed this inside?
-------------------- ~ I used to get high on life, until I realized life was cut with morons ~ * You need 2 wake up and smell the music! * -We are all computer data in a materialistic world- |Sometimes you have to lose yourself, to find anything|
 
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tryptkaloids
Learner



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Re: Cultivation General Discussion [Re: Blazer420]
#26134563 - 08/13/19 09:41 PM (4 years, 5 months ago) |
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Not impossible
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Failboat
Fuck Up

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Re: Cultivation General Discussion [Re: tryptkaloids]
#26134913 - 08/14/19 06:56 AM (4 years, 5 months ago) |
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I have found that alot with suringes left for too long, actually almost 100% if left long enough IME.
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Ferather
Mycological



Registered: 03/19/15
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Re: Cultivation General Discussion [Re: Failboat]
#26134940 - 08/14/19 07:21 AM (4 years, 5 months ago) |
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Extra research data (see previous posts, here and here):
Note: oxidative enzymes can decay more materials than just lignin.
Link - Theanine, serotonin, dopamine and more data on the oxidation of tea phenols and other compounds. Link - Nutritional composition of tea (ready to drink, agar), contains trace serotonin. Link - Enzymatic degradation of lignin in soil (extra info link here).
https://en.wikipedia.org/wiki/Theanine (psychoactive).
====
Cubensis burma, on T-Gel (tea phenols), no sugar, no starch, no cellulose (no glucose sources).

It finalized oxidation, and absorbed-converted materials, into sugars-other.
Edited by Ferather (08/14/19 07:43 AM)
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MLPismyOPSEC
That One Ponyfucker


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Re: Cultivation General Discussion [Re: Ferather]
#26135237 - 08/14/19 10:12 AM (4 years, 5 months ago) |
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n00bie question that i'm pretty sure i know the answer to, but after some searching i didn't find much conclusive talk. Why are some of my plates getting really thin myc but some are highly white and rhizo? Bottom two plates are T3, top is T2, parent plates all relatively looked the same, all are the same recipe 500mL/7.5g agar/7.5g ELME.

I believe the answer is "just keep swimming transferring, it'll come." Yes, no, maybe so?
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tryptkaloids
Learner



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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26135665 - 08/14/19 01:58 PM (4 years, 5 months ago) |
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Some cultures perform better on different types and concentrations of media. A culture can also get "lazy" on one media after awhile. Some argue that would take longer than we work with them like senescence. But we don't really know this organism the way we like to think we do, the variation of genetics is vast like humanity.
I would try lowering the nutes and if that doesn't work try a different recipe like grain water or pda.
Those cultures look good tho, I'd drop them, maybe transfer the bottom left one more time. Just always make sure you transfer from a culture before using the whole plate.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
Edited by tryptkaloids (08/14/19 01:59 PM)
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tryptkaloids
Learner



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Re: Cultivation General Discussion [Re: tryptkaloids]
#26135705 - 08/14/19 02:18 PM (4 years, 5 months ago) |
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Am I the only one baffled and amazed how water pushes final dry weight so far?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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verum subsequentis
seeker of truth



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Re: Cultivation General Discussion [Re: tryptkaloids]
#26135725 - 08/14/19 02:27 PM (4 years, 5 months ago) |
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meaning? i don't understand.
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Failboat
Fuck Up

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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26135828 - 08/14/19 03:12 PM (4 years, 5 months ago) |
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Quote:
MLPismyOPSEC said: n00bie question that i'm pretty sure i know the answer to, but after some searching i didn't find much conclusive talk. Why are some of my plates getting really thin myc but some are highly white and rhizo? Bottom two plates are T3, top is T2, parent plates all relatively looked the same, all are the same recipe 500mL/7.5g agar/7.5g ELME.

I believe the answer is "just keep swimming transferring, it'll come." Yes, no, maybe so?
It might be the light but the two T3's look more visible than the T2. If growth has changed via transfer, it's probably adapted to the media and-or setup.
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tryptkaloids
Learner



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Quote:
verum subsequentis said: meaning? i don't understand.
I mean that sometimes access to water can make or break overall yield.
I just don't understand the mechanism that allows water to increase the mass of the mushroom, even after the water is removed.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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AyePlus
Stony Danza



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Re: Cultivation General Discussion [Re: tryptkaloids]
#26136040 - 08/14/19 05:24 PM (4 years, 5 months ago) |
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Think about an animal with limited access to water but all the food in the world. Just because the food is there doesnt mean it can be digested properly. No water will kill anything faster than no food. Mushrooms are essentialy an inside out stomach with a decentralized nervous system, they need the right moisture to digest the grain/substrate.
My biggest issue has been too much water in my high humidity climate. Constant 70+% means very little fae even with a fan in the room. Been running a dehumidifier 24/7 to keep it closer to 55% but it fills up in about 12 hours. Left for a few days and came back to a bunch of bacterial blotch.
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verum subsequentis
seeker of truth



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Re: Cultivation General Discussion [Re: tryptkaloids]
#26136050 - 08/14/19 05:29 PM (4 years, 5 months ago) |
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i see. Well, given that they are roughly 90 percent water and given that conditions are so important to proper pin formation and fruit body growth... It makes a lot of sense to me
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tryptkaloids
Learner



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Aye, thanks for the wonderful explaination! Makes plenty of sense.
@verum, it makes sense, i just didn't understand how water effected dry weight if we just take it back out, but knowing it's about digestion makes even more sense
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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verum subsequentis
seeker of truth



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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#26136271 - 08/14/19 07:34 PM (4 years, 5 months ago) |
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In my opinion, these little fellers just really really do best in the proper climate. Like a fish that'll only grow as big as it's environment allows. These guys just love water.
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Mike O.Rrhizal
Tree


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As fa as ive understood mushrooms need so mush water to fruit because the mushroom itself because they grow through cell expansion not through cell division. So the mycelium will form the pin will all the cells that the mushroom needs to mature then it will fill these cells with water to grow the mushroom. And the mycelium cannot store water so can only use the water directly available to it.
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Ferather
Mycological



Registered: 03/19/15
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Rain also triggers fruiting, which means the mycelium reacts to higher water content (availability), and probably additional humidity (from evaporation). Also note, solubles require a media to be soluble, usually water. Enzymes also need water to disperse and produce solubles.
Mycelium also use water to transport solubles and other materials around, like blood for animals.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
Mike O.Rrhizal said: As fa as ive understood mushrooms need so mush water to fruit because the mushroom itself because they grow through cell expansion not through cell division. So the mycelium will form the pin will all the cells that the mushroom needs to mature then it will fill these cells with water to grow the mushroom. And the mycelium cannot store water so can only use the water directly available to it.
There's cell division in a growing mushroom
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