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Offlinestares
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Registered: 07/13/19
Posts: 18
Last seen: 4 years, 3 months
Re: Cultivation General Discussion [Re: Sherlock Shrooms]
    #26116741 - 07/18/19 07:33 PM (4 years, 6 months ago)

Rapid-fire newbie questions:

1. I did the first test run with my PC and water to learn about getting it to run smoothly, and after 30 minutes of use the area at the water line stained to dark brownish color (Aluminum material PC + tap water). What does that mean?

2. Is the purpose of Sterilzation to just clean the outside of the jars? Doesn't sound right to me, but when you cover the jars with tin foil [to prevent moisture getting in] I think it's sealing the inside right?

3. Is it ok to transfer jars from PC to SAB morning after sterilizing (no cleaning or extra step)?

4. Should I wipe down every surface (table, SAB, arms/gloves) with only 70% alcohol? Saw something about soap?

5. Syringe appears completely clear, cannot see any spore. Did I get toilet water?

6. Flame sterilize between each jar (4 holes)?

7. After I'm done innoculating, I just put the jars on a shelf?

8. Is there any big mistake that most newbies make that you can warn me about?


Edited by stares (07/18/19 07:35 PM)


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InvisibleFunky Monkey
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Re: Cultivation General Discussion [Re: Sherlock Shrooms]
    #26116744 - 07/18/19 07:34 PM (4 years, 6 months ago)



I was just getting some agar ready and went outside to find a second case of 500 celltreat petri dishes had just been delivered.

I ordered a case of 500 that got here last week, and tonight I randomly got a second case of 500 apparently by accident. Like my order was double fulfilled.

I checked my order history and I only paid for one, but a second case showed up from a different address than the first.

Weird. I kind of feel bad, but what am I gonna mail a big ass box of dishes back?


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
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Re: Cultivation General Discussion [Re: Funky Monkey]
    #26116757 - 07/18/19 07:43 PM (4 years, 6 months ago)

:score:


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InvisibleFunky Monkey
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Re: Cultivation General Discussion [Re: stares]
    #26116763 - 07/18/19 07:47 PM (4 years, 6 months ago)

Quote:

stares said:
Rapid-fire newbie questions:

1. I did the first test run with my PC and water to learn about getting it to run smoothly, and after 30 minutes of use the area at the water line stained to dark brownish color (Aluminum material PC + tap water). What does that mean?

2. Is the purpose of Sterilzation to just clean the outside of the jars? Doesn't sound right to me, but when you cover the jars with tin foil [to prevent moisture getting in] I think it's sealing the inside right?

3. Is it ok to transfer jars from PC to SAB morning after sterilizing (no cleaning or extra step)?

4. Should I wipe down every surface (table, SAB, arms/gloves) with only 70% alcohol? Saw something about soap?

5. Syringe appears completely clear, cannot see any spore. Did I get toilet water?

6. Flame sterilize between each jar (4 holes)?

7. After I'm done innoculating, I just put the jars on a shelf?

8. Is there any big mistake that most newbies make that you can warn me about?




1. Don't worry about it, PCs get discolored. You should see mine.

2. No, the purpose is to kill the contamination causing endospores in your grain before innoculating with XYZ. No, tin foil seals nothing. It keeps water from getting into your jar.

3. Yes

4. Wash first, then 70% ISO is good. Better than 91% because the water content in the 70% allows for slower evaporation causing the alcohol to hang around on the surface longer, thereby sanitizing more effectively. 91% evaporates too quick.

5. No way to know until you try it, if you got it from a reputable vendor then you are probably fine. If you were all willy-nilly on your sourcing it might be toilet water. However, sometimes you can't see them, shake it very well between every squirt. PE is notorious for this type of thing.

6. Yes. Between every jar.

7. That is fine.

8. YES! NOT USING THE SEARCH ENGINE!

As always, the surest route to success is hours upon hours of reading the extensive amount of resources all of us shroomery members have available to us through the search function. :cheers:


BODs easy AF search engine tek

Welcome to the Shroomery.


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OfflineSherlock Shrooms
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Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
Re: Cultivation General Discussion [Re: Funky Monkey]
    #26116808 - 07/18/19 08:17 PM (4 years, 6 months ago)

Hey guys. After growing for a few years now I've still never gotten a full canopy of shrooms. I have some tubs with really nice pinsets that could lead to a full canopy if I don't fuck it up. I must be going wrong somewhere along the journey by giving to much or little FAE and/or misting too much or too little, or something else entirely I'm missing. I created a thread for it here https://www.shroomery.org/forums/showflat.php/Number/26116804/vc/1#26116804 I'd love any feedback if you are willing to come check it out, thanks!


--------------------
"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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InvisibleFunky Monkey
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Re: Cultivation General Discussion [Re: Sherlock Shrooms]
    #26116841 - 07/18/19 08:33 PM (4 years, 6 months ago)

Well for starters, are you using clones, or MS?


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OfflineSherlock Shrooms
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Re: Cultivation General Discussion [Re: Funky Monkey]
    #26116870 - 07/18/19 08:49 PM (4 years, 6 months ago)

An isolate. Others suggest otherwise but I received it as a supposed isolate. It's always had the same organized growth and after taking small sections of it for about 2 years it hasn't changed. I keep it in the fridge so it hasn't been constantly aging or at least running that whole time. I have some new MS stuff about to go into jars so I'll see how new genes do. But ya, this is an isolate


--------------------
"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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OfflineSherlock Shrooms
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Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
Re: Cultivation General Discussion [Re: Sherlock Shrooms]
    #26116872 - 07/18/19 08:51 PM (4 years, 6 months ago)

Here it is  if you want to just check it out here


I have a desription of its setup and what I'm doing to it in the thread I linked above


--------------------
"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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OfflineMr. Funguy
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Registered: 01/19/19
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Re: Cultivation General Discussion [Re: Funky Monkey]
    #26116887 - 07/18/19 08:59 PM (4 years, 6 months ago)

Quote:

Funky Monkey said:


I was just getting some agar ready and went outside to find a second case of 500 celltreat petri dishes had just been delivered.

I ordered a case of 500 that got here last week, and tonight I randomly got a second case of 500 apparently by accident. Like my order was double fulfilled.

I checked my order history and I only paid for one, but a second case showed up from a different address than the first.

Weird. I kind of feel bad, but what am I gonna mail a big ass box of dishes back?






Score!!!
I'll take em off your hands... :hehehe:


--------------------


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InvisibleFunky Monkey
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Posts: 1,099
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Re: Cultivation General Discussion [Re: Sherlock Shrooms]
    #26116909 - 07/18/19 09:10 PM (4 years, 6 months ago)

We should carry this over to the thread you made for this. See you over there.


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,224
Last seen: 12 hours, 15 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Funky Monkey]
    #26117374 - 07/19/19 06:37 AM (4 years, 6 months ago)

how do you guys/gals like to organize your cult supplies?
I'm sure i am being OCD about it, but I HATE that I have things spread across the apartment.
I fruit in my room, and keep my SAB and "lab" equip in there (iso, scaple,paper towels, ect)

and in a big tote in another room is my coir, poly, grain, filter lids and odds and ends.

and in the kitchen is the agar supplies, scale, empty no pours, unused shoeboxes under the counter, PC, stock pot, and 5 gal bucket.


I cant wait to have 1 room dedicated to this. OR eventually have the space to put a nice outdoor shed I can myco pimp out


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: Funky Monkey]
    #26117426 - 07/19/19 07:08 AM (4 years, 6 months ago)

Quote:

Funky Monkey said:
Quote:

stares said:
Rapid-fire newbie questions:

1. I did the first test run with my PC and water to learn about getting it to run smoothly, and after 30 minutes of use the area at the water line stained to dark brownish color (Aluminum material PC + tap water). What does that mean?

2. Is the purpose of Sterilzation to just clean the outside of the jars? Doesn't sound right to me, but when you cover the jars with tin foil [to prevent moisture getting in] I think it's sealing the inside right?

3. Is it ok to transfer jars from PC to SAB morning after sterilizing (no cleaning or extra step)?

4. Should I wipe down every surface (table, SAB, arms/gloves) with only 70% alcohol? Saw something about soap?

5. Syringe appears completely clear, cannot see any spore. Did I get toilet water?

6. Flame sterilize between each jar (4 holes)?

7. After I'm done innoculating, I just put the jars on a shelf?

8. Is there any big mistake that most newbies make that you can warn me about?




1. Don't worry about it, PCs get discolored. You should see mine.

2. No, the purpose is to kill the contamination causing endospores in your grain before innoculating with XYZ. No, tin foil seals nothing. It keeps water from getting into your jar.

3. Yes

4. Wash first, then 70% ISO is good. Better than 91% because the water content in the 70% allows for slower evaporation causing the alcohol to hang around on the surface longer, thereby sanitizing more effectively. 91% evaporates too quick.

5. No way to know until you try it, if you got it from a reputable vendor then you are probably fine. If you were all willy-nilly on your sourcing it might be toilet water. However, sometimes you can't see them, shake it very well between every squirt. PE is notorious for this type of thing.

6. Yes. Between every jar.

7. That is fine.

8. YES! NOT USING THE SEARCH ENGINE!

As always, the surest route to success is hours upon hours of reading the extensive amount of resources all of us shroomery members have available to us through the search function. :cheers:


BODs easy AF search engine tek

Welcome to the Shroomery.




I agree with all of this except #4... I worked a dry SAB without gloves for MONTHS without issue: it's all in your sterile technique

NEVER put an explosive chemical (alcohol) into a closed space (SAB)... ESPECIALLY if you flame inside the SAB  (don't do that either...)


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InvisiblemushboyMDiscord
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Registered: 04/24/05
Posts: 32,281
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26117483 - 07/19/19 07:48 AM (4 years, 6 months ago)

Quote:

Funky Monkey said:
4. Wash first, then 70% ISO is good. Better than 91% because the water content in the 70% allows for slower evaporation





:nerd:

Quote:

bodhisatta said:
70% kills better
It has the right osmotic pressure and tonicity to penetrate cells and denature their insides. It may denature surface proteins on cell walls and bacterial envelopes but not to the extent higher percentage alcohol(both iso and ethanol) will. Alcohol is able to permeate cells and then go to work on intracellular components

A secondary benefit is it evaporates slower which potentiates it's killing ability




and yah no need to wipe out the sab with iso.


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 13 hours, 2 minutes
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26117484 - 07/19/19 07:49 AM (4 years, 6 months ago)

Read The Fucking Manual(RTFM)
      *vent your PC
:costanza:


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,224
Last seen: 12 hours, 15 minutes
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Re: Cultivation General Discussion [Re: Failboat]
    #26118435 - 07/19/19 05:13 PM (4 years, 6 months ago)

Well something germinated from the swab, but outside the streak pattern...
Maybe i knocked some spores lose.... Or maybe its mold



--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleR.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time Flag
Re: Cultivation General Discussion [Re: Camera93]
    #26118574 - 07/19/19 06:51 PM (4 years, 6 months ago)

Judgeing by where it is ( on the edge) (off the streak pattern) and how fluffy it looks on the 3rd pic, probably mold.
The reason we streak plates is so we know. if it is on the streak pattern; it is most likely suppose to be there. :thumbup:


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners


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InvisibleFunky Monkey
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Registered: 05/14/19
Posts: 1,099
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Re: Cultivation General Discussion [Re: mushboy]
    #26118590 - 07/19/19 07:04 PM (4 years, 6 months ago)

Quote:

Caps McGee said:
Quote:

Funky Monkey said:
Quote:

stares said:
Rapid-fire newbie questions:

1. I did the first test run with my PC and water to learn about getting it to run smoothly, and after 30 minutes of use the area at the water line stained to dark brownish color (Aluminum material PC + tap water). What does that mean?

2. Is the purpose of Sterilzation to just clean the outside of the jars? Doesn't sound right to me, but when you cover the jars with tin foil [to prevent moisture getting in] I think it's sealing the inside right?

3. Is it ok to transfer jars from PC to SAB morning after sterilizing (no cleaning or extra step)?

4. Should I wipe down every surface (table, SAB, arms/gloves) with only 70% alcohol? Saw something about soap?

5. Syringe appears completely clear, cannot see any spore. Did I get toilet water?

6. Flame sterilize between each jar (4 holes)?

7. After I'm done innoculating, I just put the jars on a shelf?

8. Is there any big mistake that most newbies make that you can warn me about?




1. Don't worry about it, PCs get discolored. You should see mine.

2. No, the purpose is to kill the contamination causing endospores in your grain before innoculating with XYZ. No, tin foil seals nothing. It keeps water from getting into your jar.

3. Yes

4. Wash first, then 70% ISO is good. Better than 91% because the water content in the 70% allows for slower evaporation causing the alcohol to hang around on the surface longer, thereby sanitizing more effectively. 91% evaporates too quick.

5. No way to know until you try it, if you got it from a reputable vendor then you are probably fine. If you were all willy-nilly on your sourcing it might be toilet water. However, sometimes you can't see them, shake it very well between every squirt. PE is notorious for this type of thing.

6. Yes. Between every jar.

7. That is fine.

8. YES! NOT USING THE SEARCH ENGINE!

As always, the surest route to success is hours upon hours of reading the extensive amount of resources all of us shroomery members have available to us through the search function. :cheers:


BODs easy AF search engine tek

Welcome to the Shroomery.




I agree with all of this except #4... I worked a dry SAB without gloves for MONTHS without issue: it's all in your sterile technique

NEVER put an explosive chemical (alcohol) into a closed space (SAB)... ESPECIALLY if you flame inside the SAB  (don't do that either...)



Quote:

mushboy said:
:nerd: (lol)

and yah no need to wipe out the sab with iso.





I totally agree.

In my rapid-fire responses to the "rapid-fire noobie questions" I was visualizing in my minds' eye the order of his question, like talking about wiping it out with iso, and then going from that to "...something about soap?"

I was more saying that he should WASH it out first, and more IF he was going to really want to go the extra mile and want to iso the inside out that that would be good to do then, because washing it by hand after iso application would kind of defeat that purpose. I admittedly got a little mentally turned around by the phrasing I guess.

THEN :facepalm: once I had iso in mind... I thought I would share the tidbit that 70% iso is better than 91% because I know it was counter-intuitive to me at first as well, and it all came out sounding like it was my recommendation. I should have reviewed my answer better. Woops!

As it stands, I don't iso my SAB I just make sure it's clean. Nor do I light my big-boy torch anywhere near said iso. Be careful folks!

Thanks for pointing out my mistake boys! :cheers:


Edited by Funky Monkey (07/19/19 09:28 PM)


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Offlinedmppb
On course

Registered: 11/11/11
Posts: 1,043
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Re: Cultivation General Discussion [Re: Funky Monkey]
    #26119008 - 07/20/19 12:23 AM (4 years, 6 months ago)

question

if i was to place an exhaust fan on a large greenhouse kinda place , around 3000 sq feet ; what place would you recommend placing it  , bottom ot top , and why ?


gracias


--------------------
...and when you loose control ; you'll reap the harvest you have sown.


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OfflineCasperMarbrand
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Registered: 08/24/14
Posts: 13
Last seen: 2 years, 9 months
Re: Cultivation General Discussion [Re: Funky Monkey]
    #26119090 - 07/20/19 02:44 AM (4 years, 6 months ago)

Quote:

Funky Monkey said:
Quote:

Caps McGee said:
Quote:

Funky Monkey said:
Quote:

stares said:
Rapid-fire newbie questions:

1. I did the first test run with my PC and water to learn about getting it to run smoothly, and after 30 minutes of use the area at the water line stained to dark brownish color (Aluminum material PC + tap water). What does that mean?

2. Is the purpose of Sterilzation to just clean the outside of the jars? Doesn't sound right to me, but when you cover the jars with tin foil [to prevent moisture getting in] I think it's sealing the inside right?

3. Is it ok to transfer jars from PC to SAB morning after sterilizing (no cleaning or extra step)?

4. Should I wipe down every surface (table, SAB, arms/gloves) with only 70% alcohol? Saw something about soap?

5. Syringe appears completely clear, cannot see any spore. Did I get toilet water?

6. Flame sterilize between each jar (4 holes)?

7. After I'm done innoculating, I just put the jars on a shelf?

8. Is there any big mistake that most newbies make that you can warn me about?




1. Don't worry about it, PCs get discolored. You should see mine.

2. No, the purpose is to kill the contamination causing endospores in your grain before innoculating with XYZ. No, tin foil seals nothing. It keeps water from getting into your jar.

3. Yes

4. Wash first, then 70% ISO is good. Better than 91% because the water content in the 70% allows for slower evaporation causing the alcohol to hang around on the surface longer, thereby sanitizing more effectively. 91% evaporates too quick.

5. No way to know until you try it, if you got it from a reputable vendor then you are probably fine. If you were all willy-nilly on your sourcing it might be toilet water. However, sometimes you can't see them, shake it very well between every squirt. PE is notorious for this type of thing.

6. Yes. Between every jar.

7. That is fine.

8. YES! NOT USING THE SEARCH ENGINE!

As always, the surest route to success is hours upon hours of reading the extensive amount of resources all of us shroomery members have available to us through the search function. :cheers:


BODs easy AF search engine tek

Welcome to the Shroomery.




I agree with all of this except #4... I worked a dry SAB without gloves for MONTHS without issue: it's all in your sterile technique

NEVER put an explosive chemical (alcohol) into a closed space (SAB)... ESPECIALLY if you flame inside the SAB  (don't do that either...)



Quote:

mushboy said:
:nerd: (lol)

and yah no need to wipe out the sab with iso.





I totally agree.

In my rapid-fire responses to the "rapid-fire noobie questions" I was visualizing in my minds' eye the order of his question, like talking about wiping it out with iso, and then going from that to "...something about soap?"

I was more saying that he should WASH it out first, and more IF he was going to really want to go the extra mile and want to iso the inside out that that would be good to do then, because washing it by hand after iso application would kind of defeat that purpose. I admittedly got a little mentally turned around by the phrasing I guess.

THEN :facepalm: once I had iso in mind... I thought I would share the tidbit that 70% iso is better than 91% because I know it was counter-intuitive to me at first as well, and it all came out sounding like it was my recommendation. I should have reviewed my answer better. Woops!

As it stands, I don't iso my SAB I just make sure it's clean. Nor do I light my big-boy torch anywhere near said iso. Be careful folks!

Thanks for pointing out my mistake boys! :cheers:




I'm also new and looking for clarification. When you say you don't iso your SAB but you just make sure it's clean are we talking about a visual inspection to make sure there isn't debris or are you cleaning it with something besides iso?

Thanks in advance!


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Offlinek5hd2y
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Registered: 04/13/17
Posts: 675
Last seen: 4 years, 2 months
Re: Cultivation General Discussion [Re: CasperMarbrand]
    #26119205 - 07/20/19 05:20 AM (4 years, 6 months ago)

Casper, clean your sab like you would your kitchen counter before making a meal. Same idea...clean not sterile bro! Your actions should be sterile, but it's all technique and practice.


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