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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
ShaperDreaming said: I've worked with a few swabs, I'd say half worked easy no problem, the others were a mixed bag.
A trick someone on here recommended, which worked on a stubborn swab that wasn't germing: just stick the fucker in the damn agar and twist it. I tried 3 attempts getting more and more agressive with that swab on agar but nothing! Then I stuck that shit in deep and twisted it around and it germinated. The swab is likely fucked as a result, but it worked 
Id stick the fucker in there and leave it lol.
-------------------- Trade List ๐๐๐ 6 hole Mini Monos
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AtlantaBl66dG6ng
Stranger
Registered: 07/17/19
Posts: 1
Last seen: 4 years, 5 months
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Re: Cultivation General Discussion [Re: Inocuole]
#26114493 - 07/17/19 04:47 PM (4 years, 6 months ago) |
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About to do my first large grow and log it on here post grow. Doing ten 58qt Unmod Bod Tubs. Coir\verm\gypsum.
Which strain should I pick? Thinking Ecuadorian or Mazatapec. Just looking for the fastest easiest growing strain.
Any other tips? How many dry Ozs do you normally get off 1 tub by the time it's done?
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McMitch
Stranger

Registered: 05/17/19
Posts: 6
Last seen: 4 years, 4 months
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How's everyone doing? So I'm looking for some insight, and any advice is appreciated. I'm new to this sight so I am still trying to become familiar with it. If I'm in the wrong forum for my topic just let me know where I can be redirected and that too will be appreciated.
So I borrowed some mycelium from a naturalized Psilocybe Ovoideocystidiata patch that I have been tending to, obviously twigs and things also came along with that which already had a great relationship with the ovoids. And yes, I did replenish them with a new food source. Anyway,the "TEK" that I'm using I actually found on mycotopia. Here is the link if anyone's interested. https://mycotopia.net/topic/8912-psilocybe-ovoideocystidiata-indoor-fruiting/
Sorry, I'll get to it. I rewinterized the mycelium by letting it sit in my freezer for 5 days. Then I let it thaw a little and introduced store bought hardwood chips. I then placed my "substrate" in a Tupperware dish that had holes put in bottom so water can drain properly. I chose to case it with coffee grounds, and more wood chips. The only difference being they were small enough that they worked somewhat as a filler, not quite sawdust though. It was then soaked to simulate a brief flooding, and drained.Then I layed out a layer of sphagnum moss so it covered the entire surface area of 66 QT storage tub. Tupperware was then placed in tub and lid was shut. Occasionally opening lid to introduce a little fresh air by fanning. I'm not misting all that much because it is doing it's intended job with controlling humidity level, along with temperature and everything seems to be going well so far.
I apologize for the length, but here is where I need some input. The mycelium growth in my dub tub is doing well, it has actually been running pretty aggressive. It's been 7 days today and it is on through the top of my casing, and it grew through 2 inches in my wood chip/coffee concoction. It's not totally covered, but it's breaking through in more areas than was expected. The thing is I have a peanut butter container that I also put my substrate mixture in and also a 2 liter bottle 3/4 full of it. I opened the lid of PB container and the wood chips are being consumed very fast, and it's so rhizomorphic it's just reaching out in every direction trying to get a hold of whatever it can grab. It's fascinating how fast it's taking over. Damn it, I'm sorry again for the length. I'm trying to ask if it would be ok if I introduce that to the tub, or just get something else going with that on it's own? Is it possible that I will jeopardize what's already happening in my tub if I did introduce more of this mycelium growth or should I do that, and is that beneficial in any way?It honestly looks like it's getting ready to start fruiting, inside the PB container especially. I just wanna be smart about this and not get too excited and make an irrational decision that leads to a situation I won't be able to fix. Any suggestions?
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: McMitch]
#26114714 - 07/17/19 06:40 PM (4 years, 6 months ago) |
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If it doesnโt work Iโll try dropping spore solution on a swab and sending it or just a syringe.
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 8 hours
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Re: Cultivation General Discussion [Re: McMitch]
#26114772 - 07/17/19 07:03 PM (4 years, 6 months ago) |
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Quote:
McMitch said: I'm trying to ask if it would be ok if I introduce that [Peanut Butter jar] to the tub, or just get something else going with that on it's own? Is it possible that I will jeopardize what's already happening in my tub if I did introduce more of this mycelium growth or should I do that, and is that beneficial in any way?
tl;dr version - McMitch wants to combine two containers of substrate, in which both are not fully colonized. Any issues with doing so?
I don't have an answer for you, but wanted to help by making the question concise!
Edited by MLPismyOPSEC (07/17/19 07:04 PM)
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Yesum
Furry as Fuc



Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Re: Cultivation General Discussion [Re: Legend21]
#26115369 - 07/18/19 05:40 AM (4 years, 6 months ago) |
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Quote:
Legend21 said: If it doesnโt work Iโll try dropping spore solution on a swab and sending it or just a syringe.
Even that is harder then it sounds. Try it
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Yesum]
#26115748 - 07/18/19 09:57 AM (4 years, 6 months ago) |
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How do you grow out clones without further isolating sectors from it? I'd like to take a clone and just fill new monotubs tubs from it but as soon as I take a small piece of agar from the first clone plate I imagine I'm isolating sectors or a sector from it and can no longer count on it growing like the mushroom I took the clone from. Do you have to isolate the clone down to single sectors and test them individually? or just not worry about it and it tends to work out?
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 34 minutes
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I just don't worry, be happy...
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Failboat]
#26115920 - 07/18/19 11:57 AM (4 years, 6 months ago) |
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Have you ever taken a nice looking potent clone and cut a small piece of agar from the clone dish and got a poor fruiter or super weak potency? Like a shit isolate from the abundance that were present in the initial clone
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (07/18/19 11:59 AM)
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 34 minutes
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Not that I know of, but I don't bioassay anything. If my quinea pigs don't complain then I don't have a clue.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 8 days
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Quote:
Sherlock Shrooms said: Have you ever taken a nice looking potent clone and cut a small piece of agar from the clone dish and got a poor fruiter or super weak potency? Like a shit isolate from the abundance that were present in the initial clone
I dont have any numbers to back it up, but ime the worse you'll ever experience is mediocre potency. Bunk shrooms occur from improper drying and storage.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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I've seen weak fresh mush, and fresh off the dehydrator... 40g was underwhelming AF... dried, it took nearly a half oz dose to get through the static to the next frequency lol
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TheOtherOtherBarry
Noob
Registered: 07/13/19
Posts: 76
Last seen: 4 years, 26 days
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Re: Cultivation General Discussion [Re: Caps McGee]
#26116263 - 07/18/19 03:07 PM (4 years, 6 months ago) |
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How many petri dishes/ agar jars do yโall make from a syringe when you get a new variety?
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Yesum
Furry as Fuc



Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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1 or 2. Usually 2 just incase.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 1 hour, 41 minutes
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Re: Cultivation General Discussion [Re: Yesum]
#26116296 - 07/18/19 03:26 PM (4 years, 6 months ago) |
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I'll do 4 just to have more options
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Crackatoa]
#26116341 - 07/18/19 03:56 PM (4 years, 6 months ago) |
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How the hell do folks have such luck with syringes on agar? I make soft agar for spore germination and have tried everything with syringes. Drops on the plate, a drop on the plate, a drop on the plate and streaked, syringe into a sterile shotglass then dip the loop in and streak the plate. Nothing nada. I've gone through dozens of syringes of different varieties from different sources and nothing has once germed on agar. wtf. Spore prints I have no problem with. I've gotten like 1 out of 10 syringes to take when shot into grains then I pull a grain out and drop it on agar. Maybe my profound fear of needles is killing the spores dead before they land on the agar
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Thanks yall, I'll take some clones and run with it
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Caps McGee]
#26116413 - 07/18/19 04:44 PM (4 years, 6 months ago) |
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Quote:
Caps McGee said: I've seen weak fresh mush, and fresh off the dehydrator... 40g was underwhelming AF... dried, it took nearly a half oz dose to get through the static to the next frequency lol
I've had the same experience. There was one batch of South African MS that was entirely inactive. 10g dried didn't do shit. Some of other batches from that same variety were alright. Most of the time I've only ended up with mediocre potency at worst, some rare times though I've gotten anything from profoundly weak to inactive
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Are you shaking the syringe before use?
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Caps McGee]
#26116579 - 07/18/19 05:59 PM (4 years, 6 months ago) |
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Yea shaking the shizz out of it. Then after flame sterilizing the tip cooling the tip of the syringe a bit by squirting some liquid through before I drop some on the plate. I'm fucking stumped with syringes
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (07/18/19 05:59 PM)
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